BR112016029572A2 - variantes de exoglucanases que possuem atividade melhorada e utilizações das mesmas - Google Patents

variantes de exoglucanases que possuem atividade melhorada e utilizações das mesmas

Info

Publication number
BR112016029572A2
BR112016029572A2 BR112016029572A BR112016029572A BR112016029572A2 BR 112016029572 A2 BR112016029572 A2 BR 112016029572A2 BR 112016029572 A BR112016029572 A BR 112016029572A BR 112016029572 A BR112016029572 A BR 112016029572A BR 112016029572 A2 BR112016029572 A2 BR 112016029572A2
Authority
BR
Brazil
Prior art keywords
variants
exoglucanase
enhanced activity
present
relates
Prior art date
Application number
BR112016029572A
Other languages
English (en)
Other versions
BR112016029572B1 (pt
Inventor
Margeot Antoine
Ullmann Christophe
Persillon Cécile
Ayrinhac Céline
Le Roux Françoise
Rousseau Lionel
Bonzom Olivier
Pradeau Stéphanie
Armand Sylvie
Fort Sébastien
Original Assignee
Centre Nat Rech Scient
Ifp Energies Now
Proteus
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Centre Nat Rech Scient, Ifp Energies Now, Proteus filed Critical Centre Nat Rech Scient
Publication of BR112016029572A2 publication Critical patent/BR112016029572A2/pt
Publication of BR112016029572B1 publication Critical patent/BR112016029572B1/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C12P7/08—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate
    • C12P7/10—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate substrate containing cellulosic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01091—Cellulose 1,4-beta-cellobiosidase (3.2.1.91)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Detergent Compositions (AREA)

Abstract

a presente invenção refere-se à expressão e otimização de enzimas envolvidas na degradação da biomassa lignocelulósica. a presente invenção refere-se especificamente a variantes da exoglucanase 1 de trichoderma reesei, bem como à utilização das referidas variantes com uma eficiência melhorada em métodos para quebrar a celulose e para produzir biocombustíveis.
BR112016029572-2A 2014-06-20 2015-06-12 Variantes de exoglucanases que possuem atividade melhorada e utilizações das mesmas BR112016029572B1 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
FR1455700 2014-06-20
FR1455700A FR3022557B1 (fr) 2014-06-20 2014-06-20 Variants d'exoglucanases a activite amelioree et leurs utilisations
PCT/FR2015/051556 WO2015193587A1 (fr) 2014-06-20 2015-06-12 Variants d'exoglucanases a activite amelioree et leurs utilisations

Publications (2)

Publication Number Publication Date
BR112016029572A2 true BR112016029572A2 (pt) 2017-10-24
BR112016029572B1 BR112016029572B1 (pt) 2023-11-07

Family

ID=51570606

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112016029572-2A BR112016029572B1 (pt) 2014-06-20 2015-06-12 Variantes de exoglucanases que possuem atividade melhorada e utilizações das mesmas

Country Status (13)

Country Link
US (2) US10745679B2 (pt)
EP (1) EP3158071B1 (pt)
CN (1) CN106459942B (pt)
BR (1) BR112016029572B1 (pt)
CA (1) CA2950077C (pt)
DK (1) DK3158071T3 (pt)
ES (1) ES2876268T3 (pt)
FR (2) FR3022557B1 (pt)
HR (1) HRP20211237T1 (pt)
HU (1) HUE055330T2 (pt)
PL (1) PL3158071T3 (pt)
PT (1) PT3158071T (pt)
WO (1) WO2015193587A1 (pt)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK3433358T3 (da) * 2016-03-24 2022-09-26 Novozymes As Cellobiohydrolasevarianter og polynukleotider, der koder for dem
CN107988127B (zh) * 2017-11-02 2021-09-03 南京农业大学 里氏木霉木质纤维素酶基因工程乳酸菌组合在调制优质苜蓿青贮饲料中的应用
FR3113291A1 (fr) 2020-08-06 2022-02-11 IFP Energies Nouvelles Procédé de production d’alcool par hydrolyse enzymatique et fermentation de biomasse lignocellulosique
FR3134103A1 (fr) 2022-03-30 2023-10-06 IFP Energies Nouvelles Procédé de production d’enzymes cellulolytiques et/ou hémicellulytiques

Family Cites Families (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6951719B1 (en) 1999-08-11 2005-10-04 Proteus S.A. Process for obtaining recombined nucleotide sequences in vitro, libraries of sequences and sequences thus obtained
FR2782323B1 (fr) 1998-08-12 2002-01-11 Proteus Procede de production in vitro de sequences polynucleotidiques recombinees, banques de sequences et sequences ainsi obtenues
US8637293B2 (en) * 1999-07-13 2014-01-28 Alliance For Sustainable Energy, Llc Cellobiohydrolase I enzymes
DK1578943T3 (da) * 2002-08-16 2012-01-09 Danisco Us Inc Nye variant-Hypocrea jecorina-CBH1-cellulaser
DK2617826T3 (en) * 2003-08-25 2015-10-12 Novozymes Inc Variants of glucosidhydrolase
FR2871170B1 (fr) 2004-06-07 2006-08-11 Proteus Sa Procede de determination de la charge mutationnelle d'une banque de genes obtenue par mutagenese aleatoire d'un gene d'interet et les moyens pour sa mise en oeuvre
BRPI0708949B1 (pt) 2006-03-20 2017-04-04 Novozymes As métodos para degradar ou converter um material celulósico, para produzir uma susbtância, e para produzir um polipeptídeo tendo atividade endoglucanase, e, célula microbiana recombinante
CN102482656A (zh) 2008-08-29 2012-05-30 埃欧金能源公司 具有提高的稳定性的经修饰的β-糖苷酶
FR2935986B1 (fr) 2008-09-12 2012-11-23 Inst Francais Du Petrole Variants de beta-glucosidase a activite amelioree et leurs utilisations.
EP2626421B1 (en) 2008-12-10 2014-03-19 Direvo Industrial Biotechnology GmbH Improved enzymes for biomass conversion
FI122029B (fi) 2008-12-30 2011-07-29 Ab Enzymes Oy Sieniperäiset endoglukanaasit, niiden tuotto ja käyttö
US20100304464A1 (en) 2009-04-06 2010-12-02 California Institute Of Technology Stable, functional chimeric cellobiohydrolases
CA2763836A1 (en) 2009-05-29 2010-12-02 Iogen Energy Corporation Novel beta-glucosidase enzymes
EP2491122A1 (en) * 2009-10-23 2012-08-29 Novozymes Inc. Cellobiohydrolase variants and polynucleotides encoding same
BR112013010129A2 (pt) * 2010-11-18 2016-07-05 Novozymes Inc polipeptídeo gh61 quimérico isolado, polinucleotídeo isolado, célula hospedeira, métodos para produzir um polipeptídeo gh61 quimérico, para degradas ou converter um material celulóstico, para sintetizar um produto de fermentação, para fermentar um material celulósico e para limpar ou lavar uma superfície dura ou roupa para lavar, planta transgênica, parte da planta ou célula de planta, uso do polipeptídeo gh61 quimérico, composição detergente, e, formulação de caldo ou composição de cultura de células completas
WO2012135659A2 (en) 2011-03-31 2012-10-04 Novozymes A/S Methods for enhancing the degradation or conversion of cellulosic material
CN103649308A (zh) 2011-04-28 2014-03-19 诺维信股份有限公司 具有内切葡聚糖酶活性的多肽和编码该多肽的多核苷酸
CN103797126A (zh) 2011-04-29 2014-05-14 诺维信股份有限公司 用于增强纤维素材料的降解或转化的方法
US9279163B2 (en) 2011-08-31 2016-03-08 Iogen Energy Corporation Cellobiohydrolase enzymes
FR2989385B1 (fr) 2012-04-13 2014-05-23 IFP Energies Nouvelles Procede de selection de clones de souches microbiennes ou de cellules animales, notamment pour la production de proteines
US20150315560A1 (en) * 2012-12-12 2015-11-05 Danisco Us Inc. Variants of cellobiohydrolases
BR112015013759A2 (pt) * 2012-12-12 2017-11-14 Danisco Us Inc variante isolada de uma enzima celobio-hidrolase (cbh) parental, polinucleotídeo isolado, vetor, célula hospedeira, composição detergente, aditivo alimentar, método para hidrolisar um substrato celulósico, sobrenadante de cultura celular, métodos de produção de um polipeptídeo de variante de cbh e sobrenadante de cultura celular

Also Published As

Publication number Publication date
EP3158071A1 (fr) 2017-04-26
BR112016029572B1 (pt) 2023-11-07
HUE055330T2 (hu) 2021-11-29
US10745679B2 (en) 2020-08-18
US20170152497A1 (en) 2017-06-01
DK3158071T3 (da) 2021-08-16
CA2950077A1 (fr) 2015-12-23
CN106459942B (zh) 2020-04-03
HRP20211237T1 (hr) 2022-01-07
CA2950077C (fr) 2023-07-18
EP3158071B1 (fr) 2021-05-19
ES2876268T3 (es) 2021-11-12
CN106459942A (zh) 2017-02-22
FR3022557A1 (fr) 2015-12-25
US11286472B2 (en) 2022-03-29
PL3158071T3 (pl) 2022-01-03
FR3073230A1 (fr) 2019-05-10
PT3158071T (pt) 2021-06-28
FR3022557B1 (fr) 2019-01-25
WO2015193587A1 (fr) 2015-12-23
US20200347371A1 (en) 2020-11-05

Similar Documents

Publication Publication Date Title
BR112017023560A2 (pt) composição de polímero reforçada com fibra, e, artigo.
BR112017023516A2 (pt) composição de polímero reforçado com fibra, e, artigo.
CO6331367A2 (es) Composiciones y metodos que comprenden variantes de celulasa con afinidad reducida para materiales no celulosicos
BR112017008132A2 (pt) variantes de endoglucanase fúngica, produção e uso das mesmas
BR112016015105A2 (pt) Conjugados var2csa-droga
BR112017005997A2 (pt) método
IN2014MN00989A (pt)
GT201300252A (es) Alfa glucosidasa ácida modificada con procesamiento acelerado
BR112016016539A2 (pt) micro-organismos recombinantes enriquecidos com carboidratos
BR112016000954A2 (pt) processo para fracionamento da biomassa lignocelulósica; líquor, polpa ou hidrolisado enzimático de organossolve; e uso de um líquido de tratamento para fracionamento de biomasssa lignocelulósica
BR112013031307A2 (pt) métodos para tratamento de material lignocelulósico
BR112016029572A2 (pt) variantes de exoglucanases que possuem atividade melhorada e utilizações das mesmas
BR112013022638A2 (pt) processo para a produção de açúcares hemicelulósicos fermentáveis e biomassa com baixo teor de cinzas a partir de biomassa celulósica; processo para a produção de açúcares hemicelulósicos fermentáveis e potência a partir de biomassa celulósica; e processo para a produção de açúcares hemicelulósicos fermentáveis e péletes de biomassa a partir de biomassa celulósica
BR112017008540A2 (pt) fibras de polipropileno aprimoradas, métodos para produzir as mesmas e seus usos para a produção de produtos de fibrocimento
CL2014000720A1 (es) Metodo de produccion de celulosa carboxilada fibrosa; y celulosa fibrosa, carboxilada y mercerizada.
BR112015030655A2 (pt) Variantes de xilanase e polinucleótidos que codificam as mesmas
BR112015007843A2 (pt) método para a preparação de um polímero, polímero, artigo e método de preparação de um artigo
BR112016012371A2 (pt) produção microbial de aminas graxas
BR112017008786A2 (pt) um método para fabricar polissacarídeo microfibrilado
BR112012023623A2 (pt) agentes osmóticos de polissacarídeos esterificados
BR112017028065A2 (pt) sistema de bioacabamento
BR102012023812B8 (pt) Método para a fabricação de um material absorvente fibroso
BR112018013079A2 (pt) cepas mutantes de trichoderma reesei
BR112015027968A2 (pt) expressão seletiva melhorada de transgenes em plantas produtoras de fibras
BR112015017830A2 (pt) processos e meios para aumentar a tolerância ao estresse e a biomassa em plantas

Legal Events

Date Code Title Description
B06U Preliminary requirement: requests with searches performed by other patent offices: procedure suspended [chapter 6.21 patent gazette]
B06A Patent application procedure suspended [chapter 6.1 patent gazette]
B06A Patent application procedure suspended [chapter 6.1 patent gazette]
B09A Decision: intention to grant [chapter 9.1 patent gazette]
B16A Patent or certificate of addition of invention granted [chapter 16.1 patent gazette]

Free format text: PRAZO DE VALIDADE: 20 (VINTE) ANOS CONTADOS A PARTIR DE 12/06/2015, OBSERVADAS AS CONDICOES LEGAIS