BR112016029572A2 - variantes de exoglucanases que possuem atividade melhorada e utilizações das mesmas - Google Patents

variantes de exoglucanases que possuem atividade melhorada e utilizações das mesmas

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Publication number
BR112016029572A2
BR112016029572A2 BR112016029572A BR112016029572A BR112016029572A2 BR 112016029572 A2 BR112016029572 A2 BR 112016029572A2 BR 112016029572 A BR112016029572 A BR 112016029572A BR 112016029572 A BR112016029572 A BR 112016029572A BR 112016029572 A2 BR112016029572 A2 BR 112016029572A2
Authority
BR
Brazil
Prior art keywords
variants
exoglucanase
enhanced activity
present
relates
Prior art date
Application number
BR112016029572A
Other languages
English (en)
Other versions
BR112016029572B1 (pt
Inventor
Margeot Antoine
Ullmann Christophe
Persillon Cécile
Ayrinhac Céline
Le Roux Françoise
Rousseau Lionel
Bonzom Olivier
Pradeau Stéphanie
Armand Sylvie
Fort Sébastien
Original Assignee
Centre Nat Rech Scient
Ifp Energies Now
Proteus
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Centre Nat Rech Scient, Ifp Energies Now, Proteus filed Critical Centre Nat Rech Scient
Publication of BR112016029572A2 publication Critical patent/BR112016029572A2/pt
Publication of BR112016029572B1 publication Critical patent/BR112016029572B1/pt

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C12P7/08—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate
    • C12P7/10—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate substrate containing cellulosic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01091—Cellulose 1,4-beta-cellobiosidase (3.2.1.91)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Detergent Compositions (AREA)

Abstract

a presente invenção refere-se à expressão e otimização de enzimas envolvidas na degradação da biomassa lignocelulósica. a presente invenção refere-se especificamente a variantes da exoglucanase 1 de trichoderma reesei, bem como à utilização das referidas variantes com uma eficiência melhorada em métodos para quebrar a celulose e para produzir biocombustíveis.
BR112016029572-2A 2014-06-20 2015-06-12 Variantes de exoglucanases que possuem atividade melhorada e utilizações das mesmas BR112016029572B1 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
FR1455700 2014-06-20
FR1455700A FR3022557B1 (fr) 2014-06-20 2014-06-20 Variants d'exoglucanases a activite amelioree et leurs utilisations
PCT/FR2015/051556 WO2015193587A1 (fr) 2014-06-20 2015-06-12 Variants d'exoglucanases a activite amelioree et leurs utilisations

Publications (2)

Publication Number Publication Date
BR112016029572A2 true BR112016029572A2 (pt) 2017-10-24
BR112016029572B1 BR112016029572B1 (pt) 2023-11-07

Family

ID=51570606

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112016029572-2A BR112016029572B1 (pt) 2014-06-20 2015-06-12 Variantes de exoglucanases que possuem atividade melhorada e utilizações das mesmas

Country Status (13)

Country Link
US (2) US10745679B2 (pt)
EP (1) EP3158071B1 (pt)
CN (1) CN106459942B (pt)
BR (1) BR112016029572B1 (pt)
CA (1) CA2950077C (pt)
DK (1) DK3158071T3 (pt)
ES (1) ES2876268T3 (pt)
FR (2) FR3022557B1 (pt)
HR (1) HRP20211237T1 (pt)
HU (1) HUE055330T2 (pt)
PL (1) PL3158071T3 (pt)
PT (1) PT3158071T (pt)
WO (1) WO2015193587A1 (pt)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11965189B2 (en) 2016-03-24 2024-04-23 Novozymes A/S Cellobiohydrolase variants and polynucleotides encoding same
CN107988127B (zh) * 2017-11-02 2021-09-03 南京农业大学 里氏木霉木质纤维素酶基因工程乳酸菌组合在调制优质苜蓿青贮饲料中的应用
FR3113291A1 (fr) 2020-08-06 2022-02-11 IFP Energies Nouvelles Procédé de production d’alcool par hydrolyse enzymatique et fermentation de biomasse lignocellulosique
FR3134103A1 (fr) 2022-03-30 2023-10-06 IFP Energies Nouvelles Procédé de production d’enzymes cellulolytiques et/ou hémicellulytiques

Family Cites Families (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6951719B1 (en) 1999-08-11 2005-10-04 Proteus S.A. Process for obtaining recombined nucleotide sequences in vitro, libraries of sequences and sequences thus obtained
FR2782323B1 (fr) 1998-08-12 2002-01-11 Proteus Procede de production in vitro de sequences polynucleotidiques recombinees, banques de sequences et sequences ainsi obtenues
US8637293B2 (en) * 1999-07-13 2014-01-28 Alliance For Sustainable Energy, Llc Cellobiohydrolase I enzymes
EP2322607B1 (en) * 2002-08-16 2015-09-16 Danisco US Inc. Novel variant Hyprocrea jecorina CBH1 cellulases with increase thermal stability comprising substitution or deletion at position S113
DK2377931T3 (da) * 2003-08-25 2013-07-08 Novozymes Inc Varianter af glycosidhydrolase
FR2871170B1 (fr) 2004-06-07 2006-08-11 Proteus Sa Procede de determination de la charge mutationnelle d'une banque de genes obtenue par mutagenese aleatoire d'un gene d'interet et les moyens pour sa mise en oeuvre
ES2368608T3 (es) 2006-03-20 2011-11-18 Novozymes, Inc. Polipéptidos con actividad endoglucanasa y polinucleótidos codifcando los polipéptidos.
MX2011002142A (es) 2008-08-29 2011-04-26 Iogen Energy Corp Beta-glucosidasas modificadas con estabilidad mejorada.
FR2935986B1 (fr) 2008-09-12 2012-11-23 Inst Francais Du Petrole Variants de beta-glucosidase a activite amelioree et leurs utilisations.
US8409839B2 (en) 2008-12-10 2013-04-02 Direvo Industrial Biotechnology Gmbh Polypeptides having cellobiohydrolase II activity
FI122029B (fi) 2008-12-30 2011-07-29 Ab Enzymes Oy Sieniperäiset endoglukanaasit, niiden tuotto ja käyttö
US20100304464A1 (en) 2009-04-06 2010-12-02 California Institute Of Technology Stable, functional chimeric cellobiohydrolases
US8486683B2 (en) 2009-05-29 2013-07-16 Iogen Energy Corporation Beta-glucosidase enzymes
BR112012006873A2 (pt) * 2009-10-23 2015-09-08 Novozymes Inc variante isolada, polinucleotideo isolado, metodo para produzir uma variante, planta transgenica, parte de planta ou celula d eplanta, e, metodos para degradar ou converter um materialcelulósico, para produzir um produto de fermentação, e, para fermentar um material celulósico.
DK2640833T3 (en) * 2010-11-18 2016-11-28 Novozymes Inc Chimeric polypeptides having cellulolytic enhancing ACTIVITY AND POLYNUCLEOTIDES ENCODING THEM
US9410136B2 (en) 2011-03-31 2016-08-09 Novozymes, Inc. Methods for enhancing the degradation or conversion of cellulosic material
US9926547B2 (en) 2011-04-28 2018-03-27 Novozymes, Inc. Polypeptides having endoglucanase activity and polynucleotides encoding same
US9624518B2 (en) 2011-04-29 2017-04-18 Novozymes, Inc. Methods for enhancing the degradation or conversion of cellulosic material
US9279163B2 (en) 2011-08-31 2016-03-08 Iogen Energy Corporation Cellobiohydrolase enzymes
FR2989385B1 (fr) 2012-04-13 2014-05-23 IFP Energies Nouvelles Procede de selection de clones de souches microbiennes ou de cellules animales, notamment pour la production de proteines
WO2014093282A1 (en) * 2012-12-12 2014-06-19 Danisco Us Inc. Variants of cellobiohydrolases
BR112015013646A2 (pt) * 2012-12-12 2017-11-14 Danisco Us Inc variante isolada de uma enzima celobio-hidrolase (cbh) parental, polinucleotídeo isolado, vetor, célula hospedeira, composição detergente, aditivo alimentar, método para hidrolisar um substrato celulósico, sobrenadante de cultura celular, métodos de produção de um polipeptídeo cbh variante e sobrenadante de cultura celular

Also Published As

Publication number Publication date
PT3158071T (pt) 2021-06-28
PL3158071T3 (pl) 2022-01-03
HUE055330T2 (hu) 2021-11-29
ES2876268T3 (es) 2021-11-12
US20200347371A1 (en) 2020-11-05
FR3022557A1 (fr) 2015-12-25
EP3158071A1 (fr) 2017-04-26
US10745679B2 (en) 2020-08-18
EP3158071B1 (fr) 2021-05-19
FR3073230A1 (fr) 2019-05-10
CA2950077C (fr) 2023-07-18
CN106459942B (zh) 2020-04-03
FR3022557B1 (fr) 2019-01-25
WO2015193587A1 (fr) 2015-12-23
BR112016029572B1 (pt) 2023-11-07
DK3158071T3 (da) 2021-08-16
HRP20211237T1 (hr) 2022-01-07
US20170152497A1 (en) 2017-06-01
CN106459942A (zh) 2017-02-22
CA2950077A1 (fr) 2015-12-23
US11286472B2 (en) 2022-03-29

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Legal Events

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B06U Preliminary requirement: requests with searches performed by other patent offices: procedure suspended [chapter 6.21 patent gazette]
B06A Patent application procedure suspended [chapter 6.1 patent gazette]
B06A Patent application procedure suspended [chapter 6.1 patent gazette]
B09A Decision: intention to grant [chapter 9.1 patent gazette]
B16A Patent or certificate of addition of invention granted [chapter 16.1 patent gazette]

Free format text: PRAZO DE VALIDADE: 20 (VINTE) ANOS CONTADOS A PARTIR DE 12/06/2015, OBSERVADAS AS CONDICOES LEGAIS