DK3158071T3 - Varianter af exoglucanaser med forbedret aktivitet og anvendelser deraf - Google Patents

Varianter af exoglucanaser med forbedret aktivitet og anvendelser deraf Download PDF

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Publication number
DK3158071T3
DK3158071T3 DK15732868.3T DK15732868T DK3158071T3 DK 3158071 T3 DK3158071 T3 DK 3158071T3 DK 15732868 T DK15732868 T DK 15732868T DK 3158071 T3 DK3158071 T3 DK 3158071T3
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DK
Denmark
Prior art keywords
exoglucanases
variants
improved activity
activity
improved
Prior art date
Application number
DK15732868.3T
Other languages
English (en)
Inventor
Céline Ayrinhac
Sébastien FORT
Stéphanie Pradeau
Antoine Margeot
Lionel Rousseau
Sylvie Armand
Olivier Bonzom
Roux Françoise Le
Cécile Persillon
Christophe Ullmann
Original Assignee
Ifp Energies Now
Proteus
Centre Nat Rech Scient
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ifp Energies Now, Proteus, Centre Nat Rech Scient filed Critical Ifp Energies Now
Application granted granted Critical
Publication of DK3158071T3 publication Critical patent/DK3158071T3/da

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/24Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405Glucanases
    • C12N9/2434Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/24Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405Glucanases
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00Preparation of compounds containing saccharide radicals
    • C12P19/14Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00Preparation of oxygen-containing organic compounds
    • C12P7/02Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06Ethanol, i.e. non-beverage
    • C12P7/08Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate
    • C12P7/10Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate substrate containing cellulosic material
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y302/00Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01091Cellulose 1,4-beta-cellobiosidase (3.2.1.91)
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02EREDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00Technologies for the production of fuel of non-fossil origin
    • Y02E50/10Biofuels, e.g. bio-diesel
DK15732868.3T 2014-06-20 2015-06-12 Varianter af exoglucanaser med forbedret aktivitet og anvendelser deraf DK3158071T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR1455700A FR3022557B1 (fr) 2014-06-20 2014-06-20 Variants d'exoglucanases a activite amelioree et leurs utilisations
PCT/FR2015/051556 WO2015193587A1 (fr) 2014-06-20 2015-06-12 Variants d'exoglucanases a activite amelioree et leurs utilisations

Publications (1)

Publication Number Publication Date
DK3158071T3 true DK3158071T3 (da) 2021-08-16

Family

ID=51570606

Family Applications (1)

Application Number Title Priority Date Filing Date
DK15732868.3T DK3158071T3 (da) 2014-06-20 2015-06-12 Varianter af exoglucanaser med forbedret aktivitet og anvendelser deraf

Country Status (13)

Country Link
US (2) US10745679B2 (da)
EP (1) EP3158071B1 (da)
CN (1) CN106459942B (da)
BR (1) BR112016029572B1 (da)
CA (1) CA2950077C (da)
DK (1) DK3158071T3 (da)
ES (1) ES2876268T3 (da)
FR (2) FR3022557B1 (da)
HR (1) HRP20211237T1 (da)
HU (1) HUE055330T2 (da)
PL (1) PL3158071T3 (da)
PT (1) PT3158071T (da)
WO (1) WO2015193587A1 (da)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK3433358T3 (da) * 2016-03-24 2022-09-26 Novozymes As Cellobiohydrolasevarianter og polynukleotider, der koder for dem
CN107988127B (zh) * 2017-11-02 2021-09-03 南京农业大学 里氏木霉木质纤维素酶基因工程乳酸菌组合在调制优质苜蓿青贮饲料中的应用
FR3113291A1 (fr) 2020-08-06 2022-02-11 IFP Energies Nouvelles Procédé de production d’alcool par hydrolyse enzymatique et fermentation de biomasse lignocellulosique
FR3134103A1 (fr) 2022-03-30 2023-10-06 IFP Energies Nouvelles Procédé de production d’enzymes cellulolytiques et/ou hémicellulytiques

Family Cites Families (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2782323B1 (fr) 1998-08-12 2002-01-11 Proteus Procede de production in vitro de sequences polynucleotidiques recombinees, banques de sequences et sequences ainsi obtenues
US6951719B1 (en) 1999-08-11 2005-10-04 Proteus S.A. Process for obtaining recombined nucleotide sequences in vitro, libraries of sequences and sequences thus obtained
US8637293B2 (en) * 1999-07-13 2014-01-28 Alliance For Sustainable Energy, Llc Cellobiohydrolase I enzymes
EP2305800B1 (en) * 2002-08-16 2015-05-27 Danisco US Inc. Novel variant hyprocrea jecorina CBH1 cellulases
EP2377931B1 (en) * 2003-08-25 2013-05-08 Novozymes Inc. Variants of glycoside hydrolases
FR2871170B1 (fr) 2004-06-07 2006-08-11 Proteus Sa Procede de determination de la charge mutationnelle d'une banque de genes obtenue par mutagenese aleatoire d'un gene d'interet et les moyens pour sa mise en oeuvre
CN103740674A (zh) 2006-03-20 2014-04-23 诺维信股份有限公司 具有内切葡聚糖酶活性的多肽和编码该多肽的多核苷酸
WO2010022518A1 (en) 2008-08-29 2010-03-04 Iogen Energy Corporation Modified beta-glucosidases with improved stability
FR2935986B1 (fr) 2008-09-12 2012-11-23 Inst Francais Du Petrole Variants de beta-glucosidase a activite amelioree et leurs utilisations.
WO2010066411A2 (en) 2008-12-10 2010-06-17 Direvo Industrial Biotechnology Gmbh Polypeptides having cellobiohydrolase ii activity
FI122029B (fi) 2008-12-30 2011-07-29 Ab Enzymes Oy Sieniperäiset endoglukanaasit, niiden tuotto ja käyttö
US20100304464A1 (en) 2009-04-06 2010-12-02 California Institute Of Technology Stable, functional chimeric cellobiohydrolases
CA2763836A1 (en) 2009-05-29 2010-12-02 Iogen Energy Corporation Novel beta-glucosidase enzymes
WO2011050037A1 (en) * 2009-10-23 2011-04-28 Novozymes, Inc. Cellobiohydrolase variants and polynucleotides encoding same
WO2012068509A1 (en) * 2010-11-18 2012-05-24 Novozymes, Inc. Chimeric polypeptides having cellulolytic enhancing activity and polynucleotides encoding same
WO2012135659A2 (en) 2011-03-31 2012-10-04 Novozymes A/S Methods for enhancing the degradation or conversion of cellulosic material
US9926547B2 (en) 2011-04-28 2018-03-27 Novozymes, Inc. Polypeptides having endoglucanase activity and polynucleotides encoding same
BR112013027463A2 (pt) 2011-04-29 2017-09-26 Novozymes Inc métodos para degradar ou converter, e para fermentar um material celulóliso, para produzir um produto de fermentação, e para limpar ou lavar uma superfície dura ou roupa suja, e, composição de detergente
US9279163B2 (en) 2011-08-31 2016-03-08 Iogen Energy Corporation Cellobiohydrolase enzymes
FR2989385B1 (fr) 2012-04-13 2014-05-23 IFP Energies Nouvelles Procede de selection de clones de souches microbiennes ou de cellules animales, notamment pour la production de proteines
KR20150093729A (ko) * 2012-12-12 2015-08-18 다니스코 유에스 인크. 셀로바이오하이드롤라제의 변이체
US20160122735A1 (en) * 2012-12-12 2016-05-05 Danisco Us Inc. Variants of cellobiohydrolases

Also Published As

Publication number Publication date
HRP20211237T1 (hr) 2022-01-07
BR112016029572B1 (pt) 2023-11-07
CN106459942B (zh) 2020-04-03
US20170152497A1 (en) 2017-06-01
HUE055330T2 (hu) 2021-11-29
US11286472B2 (en) 2022-03-29
EP3158071B1 (fr) 2021-05-19
FR3073230A1 (fr) 2019-05-10
CN106459942A (zh) 2017-02-22
PL3158071T3 (pl) 2022-01-03
CA2950077C (fr) 2023-07-18
ES2876268T3 (es) 2021-11-12
US10745679B2 (en) 2020-08-18
FR3022557A1 (fr) 2015-12-25
CA2950077A1 (fr) 2015-12-23
US20200347371A1 (en) 2020-11-05
EP3158071A1 (fr) 2017-04-26
PT3158071T (pt) 2021-06-28
BR112016029572A2 (pt) 2017-10-24
FR3022557B1 (fr) 2019-01-25
WO2015193587A1 (fr) 2015-12-23

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