BR112016026869A2 - nitrila hidratase melhorada - Google Patents

nitrila hidratase melhorada

Info

Publication number
BR112016026869A2
BR112016026869A2 BR112016026869A BR112016026869A BR112016026869A2 BR 112016026869 A2 BR112016026869 A2 BR 112016026869A2 BR 112016026869 A BR112016026869 A BR 112016026869A BR 112016026869 A BR112016026869 A BR 112016026869A BR 112016026869 A2 BR112016026869 A2 BR 112016026869A2
Authority
BR
Brazil
Prior art keywords
amino acid
valine
threonine
leucine
nitrile hydratase
Prior art date
Application number
BR112016026869A
Other languages
English (en)
Other versions
BR112016026869B1 (pt
Inventor
Kitahara Aya
Watanabe Fumiaki
Wakamatsu Miki
Akiyama Takanori
Original Assignee
Mitsubishi Rayon Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Mitsubishi Rayon Co filed Critical Mitsubishi Rayon Co
Publication of BR112016026869A2 publication Critical patent/BR112016026869A2/pt
Publication of BR112016026869B1 publication Critical patent/BR112016026869B1/pt

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/02—Amides, e.g. chloramphenicol or polyamides; Imides or polyimides; Urethanes, i.e. compounds comprising N-C=O structural element or polyurethanes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y402/00—Carbon-oxygen lyases (4.2)
    • C12Y402/01—Hydro-lyases (4.2.1)
    • C12Y402/01084—Nitrile hydratase (4.2.1.84)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

a presente invenção refere-se a nova nitrila hidratase melhorada com maior resistência a compostos de amida sob altas temperaturas. especificamente é fornecida uma nitrila hidratase tendo pelo menos um aminoácido selecionado de (a) a (d) abaixo, na sequência de aminoácidos expressada em seq id no:50 (x1 a x27 representam resíduos de aminoácidos arbitrariamente definidos independentes). (a) x1 é valina ou glicina, (b) x9 é valina ou treonina, (c) x23 é um aminoácido selecionado a partir de um grupo composto de isoleucina, leucina, metionina e treonina, (d) x24 é leucina.
BR112016026869-5A 2014-06-06 2015-05-12 Nitrila hidratase melhorada e método para a produção de um composto de amida BR112016026869B1 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2014-118041 2014-06-06
JP2014118041 2014-06-06
PCT/JP2015/002396 WO2015186298A1 (ja) 2014-06-06 2015-05-12 改良型ニトリルヒドラターゼ

Publications (2)

Publication Number Publication Date
BR112016026869A2 true BR112016026869A2 (pt) 2017-12-12
BR112016026869B1 BR112016026869B1 (pt) 2023-06-06

Family

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Also Published As

Publication number Publication date
RU2689606C2 (ru) 2019-05-28
US10093912B2 (en) 2018-10-09
CN116536293A (zh) 2023-08-04
CN106459962A (zh) 2017-02-22
JP2019088327A (ja) 2019-06-13
CN112941060A (zh) 2021-06-11
EP3153582A4 (en) 2017-06-14
EP3153582B1 (en) 2020-06-17
WO2015186298A1 (ja) 2015-12-10
CN116656657A (zh) 2023-08-29
US20170283784A1 (en) 2017-10-05
AU2015270032A1 (en) 2017-01-12
JP6690747B2 (ja) 2020-04-28
JP6489013B2 (ja) 2019-03-27
JPWO2015186298A1 (ja) 2017-04-20
RU2016152249A3 (pt) 2018-07-10
CN110079516B (zh) 2023-02-24
AU2015270032B2 (en) 2018-11-29
JP6741093B2 (ja) 2020-08-19
KR20160145183A (ko) 2016-12-19
JP2019088326A (ja) 2019-06-13
KR101995103B1 (ko) 2019-07-03
EP3153582A1 (en) 2017-04-12
CN110079516A (zh) 2019-08-02
RU2016152249A (ru) 2018-07-10

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Legal Events

Date Code Title Description
B25D Requested change of name of applicant approved

Owner name: MITSUBISHI CHEMICAL CORPORATION (JP)

B06U Preliminary requirement: requests with searches performed by other patent offices: procedure suspended [chapter 6.21 patent gazette]
B350 Update of information on the portal [chapter 15.35 patent gazette]
B350 Update of information on the portal [chapter 15.35 patent gazette]
B06A Patent application procedure suspended [chapter 6.1 patent gazette]
B09A Decision: intention to grant [chapter 9.1 patent gazette]
B16A Patent or certificate of addition of invention granted [chapter 16.1 patent gazette]

Free format text: PRAZO DE VALIDADE: 20 (VINTE) ANOS CONTADOS A PARTIR DE 12/05/2015, OBSERVADAS AS CONDICOES LEGAIS