BR112016023814B1 - Processos para a produção de uma mutação em uma região de mutagênese pretendida (imr) dentro de um genoma bacteriano e para a produção de uma bactéria mutada - Google Patents

Processos para a produção de uma mutação em uma região de mutagênese pretendida (imr) dentro de um genoma bacteriano e para a produção de uma bactéria mutada Download PDF

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Publication number
BR112016023814B1
BR112016023814B1 BR112016023814-1A BR112016023814A BR112016023814B1 BR 112016023814 B1 BR112016023814 B1 BR 112016023814B1 BR 112016023814 A BR112016023814 A BR 112016023814A BR 112016023814 B1 BR112016023814 B1 BR 112016023814B1
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bacteria
pam
protospacer
imr
crispr
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BR112016023814-1A
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BR112016023814A2 (pt
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Elizabeth Jenkinson
Preben Krabben
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Green Biologics Limited
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2800/00—Nucleic acids vectors
    • C12N2800/10—Plasmid DNA
    • C12N2800/101—Plasmid DNA for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2830/00—Vector systems having a special element relevant for transcription
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

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  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
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  • Apparatus Associated With Microorganisms And Enzymes (AREA)
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BR112016023814-1A 2014-04-17 2015-04-16 Processos para a produção de uma mutação em uma região de mutagênese pretendida (imr) dentro de um genoma bacteriano e para a produção de uma bactéria mutada BR112016023814B1 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB1406970.2 2014-04-17
GBGB1406970.2A GB201406970D0 (en) 2014-04-17 2014-04-17 Targeted mutations
PCT/GB2015/051153 WO2015159087A1 (en) 2014-04-17 2015-04-16 Targeted mutations

Publications (2)

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BR112016023814A2 BR112016023814A2 (pt) 2017-10-17
BR112016023814B1 true BR112016023814B1 (pt) 2023-02-07

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BR112016023814-1A BR112016023814B1 (pt) 2014-04-17 2015-04-16 Processos para a produção de uma mutação em uma região de mutagênese pretendida (imr) dentro de um genoma bacteriano e para a produção de uma bactéria mutada

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US (1) US10508271B2 (https=)
EP (1) EP3132036B8 (https=)
JP (1) JP6621465B2 (https=)
KR (1) KR102272684B1 (https=)
CN (1) CN106460000B (https=)
BR (1) BR112016023814B1 (https=)
CA (1) CA2945574C (https=)
DK (1) DK3132036T3 (https=)
GB (2) GB201406970D0 (https=)
MY (1) MY176328A (https=)
WO (1) WO2015159087A1 (https=)

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Publication number Publication date
EP3132036B1 (en) 2020-01-15
GB201406970D0 (en) 2014-06-04
CN106460000B (zh) 2019-12-27
EP3132036B8 (en) 2020-04-22
CN106460000A (zh) 2017-02-22
KR102272684B1 (ko) 2021-07-02
CA2945574C (en) 2022-10-25
US20170037393A1 (en) 2017-02-09
US10508271B2 (en) 2019-12-17
GB2530831B (en) 2016-11-30
BR112016023814A2 (pt) 2017-10-17
JP6621465B2 (ja) 2019-12-18
MY176328A (en) 2020-07-29
KR20160147844A (ko) 2016-12-23
DK3132036T3 (da) 2020-03-16
GB201506460D0 (en) 2015-06-03
EP3132036A1 (en) 2017-02-22
CA2945574A1 (en) 2015-10-22
JP2017511156A (ja) 2017-04-20
WO2015159087A1 (en) 2015-10-22
GB2530831A (en) 2016-04-06

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