BR112016023814A2 - mutações visadas - Google Patents

mutações visadas

Info

Publication number
BR112016023814A2
BR112016023814A2 BR112016023814A BR112016023814A BR112016023814A2 BR 112016023814 A2 BR112016023814 A2 BR 112016023814A2 BR 112016023814 A BR112016023814 A BR 112016023814A BR 112016023814 A BR112016023814 A BR 112016023814A BR 112016023814 A2 BR112016023814 A2 BR 112016023814A2
Authority
BR
Brazil
Prior art keywords
targeted mutations
desired mutation
bacteria
relates
recombination
Prior art date
Application number
BR112016023814A
Other languages
English (en)
Other versions
BR112016023814B1 (pt
Inventor
Jenkinson Elizabeth
Krabben Preben
Original Assignee
Green Biologics Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Green Biologics Ltd filed Critical Green Biologics Ltd
Publication of BR112016023814A2 publication Critical patent/BR112016023814A2/pt
Publication of BR112016023814B1 publication Critical patent/BR112016023814B1/pt

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/10—Plasmid DNA
    • C12N2800/101—Plasmid DNA for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Mycology (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

a presente invenção refere-se a um processo para a produção e a seleção de mutações visadas em genomas bacterianos. em particular, o processo refere-se à transformação das bactérias com um elemento de recombinação que compreende a mutação desejada seguida pela recombinação homóloga do elemento de recombinação no genoma bacteriano; o sistema crispr/cas é usado então para eliminar as bactérias que não têm a mutação desejada.
BR112016023814-1A 2014-04-17 2015-04-16 Processos para a produção de uma mutação em uma região de mutagênese pretendida (imr) dentro de um genoma bacteriano e para a produção de uma bactéria mutada BR112016023814B1 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB1406970.2 2014-04-17
GBGB1406970.2A GB201406970D0 (en) 2014-04-17 2014-04-17 Targeted mutations
PCT/GB2015/051153 WO2015159087A1 (en) 2014-04-17 2015-04-16 Targeted mutations

Publications (2)

Publication Number Publication Date
BR112016023814A2 true BR112016023814A2 (pt) 2017-10-17
BR112016023814B1 BR112016023814B1 (pt) 2023-02-07

Family

ID=50928941

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112016023814-1A BR112016023814B1 (pt) 2014-04-17 2015-04-16 Processos para a produção de uma mutação em uma região de mutagênese pretendida (imr) dentro de um genoma bacteriano e para a produção de uma bactéria mutada

Country Status (11)

Country Link
US (1) US10508271B2 (pt)
EP (1) EP3132036B8 (pt)
JP (1) JP6621465B2 (pt)
KR (1) KR102272684B1 (pt)
CN (1) CN106460000B (pt)
BR (1) BR112016023814B1 (pt)
CA (1) CA2945574C (pt)
DK (1) DK3132036T3 (pt)
GB (2) GB201406970D0 (pt)
MY (1) MY176328A (pt)
WO (1) WO2015159087A1 (pt)

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SG11201903089RA (en) 2016-10-14 2019-05-30 Harvard College Aav delivery of nucleobase editors
WO2018119359A1 (en) 2016-12-23 2018-06-28 President And Fellows Of Harvard College Editing of ccr5 receptor gene to protect against hiv infection
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Also Published As

Publication number Publication date
GB201406970D0 (en) 2014-06-04
EP3132036B1 (en) 2020-01-15
EP3132036A1 (en) 2017-02-22
BR112016023814B1 (pt) 2023-02-07
GB2530831A (en) 2016-04-06
GB2530831B (en) 2016-11-30
JP6621465B2 (ja) 2019-12-18
US10508271B2 (en) 2019-12-17
DK3132036T3 (da) 2020-03-16
WO2015159087A1 (en) 2015-10-22
CN106460000B (zh) 2019-12-27
CA2945574C (en) 2022-10-25
JP2017511156A (ja) 2017-04-20
EP3132036B8 (en) 2020-04-22
GB201506460D0 (en) 2015-06-03
KR102272684B1 (ko) 2021-07-02
KR20160147844A (ko) 2016-12-23
CA2945574A1 (en) 2015-10-22
MY176328A (en) 2020-07-29
US20170037393A1 (en) 2017-02-09
CN106460000A (zh) 2017-02-22

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Owner name: GREEN BIOLOGICS LIMITED (GB)

B06U Preliminary requirement: requests with searches performed by other patent offices: procedure suspended [chapter 6.21 patent gazette]
B350 Update of information on the portal [chapter 15.35 patent gazette]
B09A Decision: intention to grant [chapter 9.1 patent gazette]
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Free format text: PRAZO DE VALIDADE: 20 (VINTE) ANOS CONTADOS A PARTIR DE 16/04/2015, OBSERVADAS AS CONDICOES LEGAIS