BR112015010138A2 - engenharia bacteriana - Google Patents

engenharia bacteriana

Info

Publication number
BR112015010138A2
BR112015010138A2 BR112015010138A BR112015010138A BR112015010138A2 BR 112015010138 A2 BR112015010138 A2 BR 112015010138A2 BR 112015010138 A BR112015010138 A BR 112015010138A BR 112015010138 A BR112015010138 A BR 112015010138A BR 112015010138 A2 BR112015010138 A2 BR 112015010138A2
Authority
BR
Brazil
Prior art keywords
growth
under
tna
condition
survival
Prior art date
Application number
BR112015010138A
Other languages
English (en)
Inventor
Keith Turner Arthur
Hugh Williams David
Richard Wain John
Original Assignee
Bactevo Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bactevo Ltd filed Critical Bactevo Ltd
Publication of BR112015010138A2 publication Critical patent/BR112015010138A2/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1058—Directional evolution of libraries, e.g. evolution of libraries is achieved by mutagenesis and screening or selection of mixed population of organisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1082—Preparation or screening gene libraries by chromosomal integration of polynucleotide sequences, HR-, site-specific-recombination, transposons, viral vectors

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Virology (AREA)
  • Ecology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

resumo da patente de invenã§ã£o para: “engenharia bacteriana”. descreve-se um processo para a produã§ã£o de uma bactã©ria mutante que exibe uma sobrevivãªncia e/ou crescimento melhorados sob uma condiã§ã£o de crescimento selecionado, o processo compreendendo as etapas de: (a) geraã§ã£o de um pool de bactã©rias mutantes por mutagãªnese de transpã³son com um transpã³son de ativaã§ã£o (tna), em que o tna compreende um promotor capaz de aumentar a transcriã§ã£o de um gene no prã³ximo ou ao local de inserã§ã£o; (b) crescimento de bactã©rias do pool mutante sob a condiã§ã£o de crescimento selecionada, e com uma ou mais condiã§ãµes de referãªncia para produzir duas ou mais culturas de ensaio; e (c) comparaã§ã£o da distribuiã§ã£o das inserã§ãµes tna entre as culturas de ensaio para identificar uma primeira classe de genes que sã£o desvantajosos para o crescimento e/ou sobrevivãªncia sob a condiã§ã£o de crescimento selecionadas, e uma segunda classe de genes que sã£o vantajosps para o crescimento e/ou sobrevivãªncia sob a condiã§ã£o de crescimento selecionada.
BR112015010138A 2012-11-06 2013-11-05 engenharia bacteriana BR112015010138A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB1219989.9A GB201219989D0 (en) 2012-11-06 2012-11-06 Bacterial engineering
PCT/GB2013/052893 WO2014072697A1 (en) 2012-11-06 2013-11-05 Bacterial engineering

Publications (1)

Publication Number Publication Date
BR112015010138A2 true BR112015010138A2 (pt) 2017-07-11

Family

ID=47429262

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112015010138A BR112015010138A2 (pt) 2012-11-06 2013-11-05 engenharia bacteriana

Country Status (12)

Country Link
US (3) US20150307873A1 (pt)
EP (1) EP2917346B1 (pt)
JP (1) JP2015533289A (pt)
KR (1) KR20150083895A (pt)
CN (1) CN104903446A (pt)
AU (1) AU2013343274A1 (pt)
BR (1) BR112015010138A2 (pt)
CA (1) CA2890104A1 (pt)
GB (1) GB201219989D0 (pt)
RU (1) RU2015119379A (pt)
SG (2) SG11201503233UA (pt)
WO (1) WO2014072697A1 (pt)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB201107515D0 (en) 2011-05-05 2011-06-22 Discuva Ltd Method for identifying antibiotic targets
GB201413202D0 (en) * 2014-07-25 2014-09-10 Discuva Ltd Process for producing bacterial mutants
GB201413198D0 (en) * 2014-07-25 2014-09-10 Discuva Ltd Methods For Characterizing Bacterial Mutants
GB201421850D0 (en) * 2014-12-09 2015-01-21 Bactevo Ltd Method for screening for bioactive natural products
US10280348B2 (en) 2017-01-31 2019-05-07 Prc-Desoto International, Inc. Low density aerospace compositions and sealants
CA3064607A1 (en) * 2017-06-06 2018-12-13 Zymergen Inc. High throughput transposon mutagenesis

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9917531D0 (en) 1999-07-26 1999-09-29 Arrow Therapeutics Ltd Transposon mediated differential hybridisation
KR100459870B1 (ko) * 2002-02-22 2004-12-04 한국과학기술원 트랜스포존과 Cre/loxP 부위 특이적 재조합 방법을 이용하는 염색체의 특정부위가 제거된 미생물 변이주 제조방법
EP1968994B1 (en) 2005-12-06 2013-07-03 Synthetic Genomics, Inc. Synthetic genomes
GB201107515D0 (en) * 2011-05-05 2011-06-22 Discuva Ltd Method for identifying antibiotic targets

Also Published As

Publication number Publication date
EP2917346A1 (en) 2015-09-16
SG11201503233UA (en) 2015-05-28
US20180135041A1 (en) 2018-05-17
AU2013343274A1 (en) 2015-06-11
EP2917346B1 (en) 2018-06-06
GB201219989D0 (en) 2012-12-19
RU2015119379A (ru) 2016-12-27
WO2014072697A1 (en) 2014-05-15
CA2890104A1 (en) 2014-05-15
JP2015533289A (ja) 2015-11-24
KR20150083895A (ko) 2015-07-20
SG10201703558XA (en) 2017-06-29
US20220403373A1 (en) 2022-12-22
CN104903446A (zh) 2015-09-09
US20150307873A1 (en) 2015-10-29

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Legal Events

Date Code Title Description
B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B06I Publication of requirement cancelled [chapter 6.9 patent gazette]

Free format text: ANULADA A PUBLICACAO CODIGO 6.6.1 NA RPI NO 2462 DE 13/03/2018 POR TER SIDO INDEVIDA.

B08F Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette]

Free format text: REFERENTE A 6A ANUIDADE.

B08K Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette]

Free format text: EM VIRTUDE DO ARQUIVAMENTO PUBLICADO NA RPI 2539 DE 03-09-2019 E CONSIDERANDO AUSENCIA DE MANIFESTACAO DENTRO DOS PRAZOS LEGAIS, INFORMO QUE CABE SER MANTIDO O ARQUIVAMENTO DO PEDIDO DE PATENTE, CONFORME O DISPOSTO NO ARTIGO 12, DA RESOLUCAO 113/2013.