BR112015002918B8 - Construção de ácido nucleico compreendeendo uma região não traduzida, vetor e métodos para produção de um peptídeo ou uma proteína e para a expressão de um gene em uma planta ou células vegetais - Google Patents
Construção de ácido nucleico compreendeendo uma região não traduzida, vetor e métodos para produção de um peptídeo ou uma proteína e para a expressão de um gene em uma planta ou células vegetaisInfo
- Publication number
- BR112015002918B8 BR112015002918B8 BR112015002918A BR112015002918A BR112015002918B8 BR 112015002918 B8 BR112015002918 B8 BR 112015002918B8 BR 112015002918 A BR112015002918 A BR 112015002918A BR 112015002918 A BR112015002918 A BR 112015002918A BR 112015002918 B8 BR112015002918 B8 BR 112015002918B8
- Authority
- BR
- Brazil
- Prior art keywords
- plant
- gene
- nucleic acid
- vector
- peptide
- Prior art date
Links
- 108090000623 proteins and genes Proteins 0.000 title abstract 9
- 239000013598 vector Substances 0.000 title abstract 5
- 238000000034 method Methods 0.000 title abstract 4
- 150000007523 nucleic acids Chemical class 0.000 title abstract 4
- 102000004169 proteins and genes Human genes 0.000 title abstract 3
- 108091023045 Untranslated Region Proteins 0.000 title abstract 2
- 108020004707 nucleic acids Proteins 0.000 title abstract 2
- 102000039446 nucleic acids Human genes 0.000 title abstract 2
- 108090000765 processed proteins & peptides Proteins 0.000 title abstract 2
- 238000010276 construction Methods 0.000 title 1
- 108091026890 Coding region Proteins 0.000 abstract 3
- 230000009261 transgenic effect Effects 0.000 abstract 3
- 108091028043 Nucleic acid sequence Proteins 0.000 abstract 1
- 102000044159 Ubiquitin Human genes 0.000 abstract 1
- 108090000848 Ubiquitin Proteins 0.000 abstract 1
- 239000012634 fragment Substances 0.000 abstract 1
- 238000010363 gene targeting Methods 0.000 abstract 1
- 230000000749 insecticidal effect Effects 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
- C12N15/8218—Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
- C12N15/821—Non-antibiotic resistance markers, e.g. morphogenetic, metabolic markers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
- C12N15/8286—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for insect resistance
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0069—Oxidoreductases (1.) acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Cell Biology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Virology (AREA)
- Insects & Arthropods (AREA)
- Pest Control & Pesticides (AREA)
- Medicinal Chemistry (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201261684255P | 2012-08-17 | 2012-08-17 | |
| US61/684,255 | 2012-08-17 | ||
| PCT/US2013/054102 WO2014028295A2 (en) | 2012-08-17 | 2013-08-08 | Use of a maize untranslated region for transgene expression in plants |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| BR112015002918A2 BR112015002918A2 (pt) | 2018-04-17 |
| BR112015002918B1 BR112015002918B1 (pt) | 2022-09-27 |
| BR112015002918B8 true BR112015002918B8 (pt) | 2023-05-16 |
Family
ID=49029217
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BR112015002918A BR112015002918B8 (pt) | 2012-08-17 | 2013-08-08 | Construção de ácido nucleico compreendeendo uma região não traduzida, vetor e métodos para produção de um peptídeo ou uma proteína e para a expressão de um gene em uma planta ou células vegetais |
Country Status (14)
| Country | Link |
|---|---|
| US (1) | US9688996B2 (https=) |
| EP (1) | EP2885412B1 (https=) |
| JP (1) | JP2015524672A (https=) |
| KR (1) | KR20150041788A (https=) |
| CN (1) | CN104662156A (https=) |
| AR (1) | AR092152A1 (https=) |
| AU (1) | AU2013302947B2 (https=) |
| BR (1) | BR112015002918B8 (https=) |
| CA (2) | CA3116898C (https=) |
| IL (1) | IL237206A0 (https=) |
| IN (1) | IN2015DN00512A (https=) |
| RU (1) | RU2015109129A (https=) |
| WO (1) | WO2014028295A2 (https=) |
| ZA (1) | ZA201500460B (https=) |
Families Citing this family (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108026149B (zh) | 2015-08-17 | 2022-04-29 | 美国陶氏益农公司 | 工程化的cry6a杀虫蛋白 |
| TW201718861A (zh) * | 2015-09-22 | 2017-06-01 | 道禮責任有限公司 | 用於轉殖基因表現之植物啟動子及3’utr |
| TW201718862A (zh) * | 2015-09-22 | 2017-06-01 | Dow Agrosciences Llc | 用於轉殖基因表現之植物啟動子及3’utr |
| WO2017059902A1 (en) | 2015-10-07 | 2017-04-13 | Biontech Rna Pharmaceuticals Gmbh | 3' utr sequences for stabilization of rna |
| TW201736600A (zh) * | 2016-03-11 | 2017-10-16 | 道禮責任有限公司 | 用於轉殖基因表現之植物啟動子及3’utr |
| US10273494B2 (en) * | 2016-05-02 | 2019-04-30 | Dow Agrosciences Llc | Plant promoter and 3′UTR for transgene expression |
| CN106845154B (zh) * | 2016-12-29 | 2022-04-08 | 浙江安诺优达生物科技有限公司 | 一种用于ffpe样本拷贝数变异检测的装置 |
| US11499159B2 (en) | 2019-01-10 | 2022-11-15 | Monsanto Technology Llc | Plant regulatory elements and uses thereof |
| MX2024005844A (es) * | 2021-11-16 | 2024-05-27 | Pioneer Hi Bred Int | Evento de maiz das-01131-3 y metodos para la deteccion de este. |
| CN116731142B (zh) * | 2023-07-24 | 2024-09-20 | 扬州大学 | ZmSAUR21基因、蛋白及其在调控苗期玉米根系发育中的应用 |
| CN118109503B (zh) * | 2024-04-16 | 2025-04-25 | 福建农林大学 | 大豆生长素酰胺合成酶gh3基因家族及其应用 |
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-
2013
- 2013-08-08 KR KR1020157003826A patent/KR20150041788A/ko not_active Withdrawn
- 2013-08-08 CA CA3116898A patent/CA3116898C/en active Active
- 2013-08-08 CN CN201380043853.7A patent/CN104662156A/zh active Pending
- 2013-08-08 AU AU2013302947A patent/AU2013302947B2/en active Active
- 2013-08-08 US US14/416,811 patent/US9688996B2/en active Active
- 2013-08-08 BR BR112015002918A patent/BR112015002918B8/pt active IP Right Grant
- 2013-08-08 IN IN512DEN2015 patent/IN2015DN00512A/en unknown
- 2013-08-08 CA CA2884256A patent/CA2884256C/en active Active
- 2013-08-08 RU RU2015109129A patent/RU2015109129A/ru not_active Application Discontinuation
- 2013-08-08 JP JP2015527493A patent/JP2015524672A/ja active Pending
- 2013-08-08 EP EP13752757.8A patent/EP2885412B1/en active Active
- 2013-08-08 WO PCT/US2013/054102 patent/WO2014028295A2/en not_active Ceased
- 2013-08-16 AR ARP130102914A patent/AR092152A1/es active IP Right Grant
-
2015
- 2015-01-22 ZA ZA2015/00460A patent/ZA201500460B/en unknown
- 2015-02-12 IL IL237206A patent/IL237206A0/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| BR112015002918B1 (pt) | 2022-09-27 |
| US20150203856A1 (en) | 2015-07-23 |
| KR20150041788A (ko) | 2015-04-17 |
| EP2885412B1 (en) | 2019-05-01 |
| CA3116898A1 (en) | 2014-02-20 |
| WO2014028295A3 (en) | 2014-04-10 |
| BR112015002918A2 (pt) | 2018-04-17 |
| IL237206A0 (en) | 2015-04-30 |
| RU2015109129A (ru) | 2016-10-10 |
| IN2015DN00512A (https=) | 2015-06-26 |
| US9688996B2 (en) | 2017-06-27 |
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| ZA201500460B (en) | 2016-10-26 |
| AU2013302947B2 (en) | 2017-06-01 |
| CN104662156A (zh) | 2015-05-27 |
| WO2014028295A2 (en) | 2014-02-20 |
| CA2884256C (en) | 2021-08-03 |
| JP2015524672A (ja) | 2015-08-27 |
| AU2013302947A1 (en) | 2015-02-05 |
| CA3116898C (en) | 2022-09-27 |
| AR092152A1 (es) | 2015-03-25 |
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