BR112014025946B8 - Molécula de ácido nucleico e proteína quimérica compreendendo um peptídeo de trânsito de cloroplasto - Google Patents

Molécula de ácido nucleico e proteína quimérica compreendendo um peptídeo de trânsito de cloroplasto

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Publication number
BR112014025946B8
BR112014025946B8 BR112014025946A BR112014025946A BR112014025946B8 BR 112014025946 B8 BR112014025946 B8 BR 112014025946B8 BR 112014025946 A BR112014025946 A BR 112014025946A BR 112014025946 A BR112014025946 A BR 112014025946A BR 112014025946 B8 BR112014025946 B8 BR 112014025946B8
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BR
Brazil
Prior art keywords
nucleic acid
transit peptide
methods
chloroplast transit
transgenic plant
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Application number
BR112014025946A
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English (en)
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BR112014025946A2 (pt
BR112014025946B1 (pt
Inventor
M Lira Justin
M Cicchillo Robert
e robinson Andrew
Yerkes Carla
Original Assignee
Dow Agrosciences Llc
Corteva Agriscience Llc
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Publication date
Application filed by Dow Agrosciences Llc, Corteva Agriscience Llc filed Critical Dow Agrosciences Llc
Publication of BR112014025946A2 publication Critical patent/BR112014025946A2/pt
Publication of BR112014025946B1 publication Critical patent/BR112014025946B1/pt
Publication of BR112014025946B8 publication Critical patent/BR112014025946B8/pt

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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C12N15/8275Glyphosate
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8214Plastid transformation
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8221Transit peptides
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Botany (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Physiology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

POLIPEPTÍDEOS QUIMÉRICOS COMPREENDENDO UM PEPTÍDEO DE TRÂNSITO DE CLAOROPLASTO, MOLÉCULAS DE ÁCIDO NUCLEICO CODIFICANDO OS MESMOS, VETORES DE EXPRESSÃO EM PLANTA, MÉTODOS PARA PRODUÇÃO DE UMA PLANTA TRANSGÊNICA E UM MATERIAL DA MESMA, SEUS USOS, BEM COMO PRODUTOS DE CONSUMO DE PLANTA TRANSGÊNICA. A presente invenção refere-se a composições e métodos para direcionamento de peptídeos, polipeptídeos e proteínas a plastídeos de células contendo plastídeo. Em algumas modalidades, a invenção refere-se a peptídeos de trânsito de cloroplasto que podem direcionar um polipeptídeo a um plastídeo, e moléculas de ácido nucleico codificando os mesmos. Em algumas modalidades, a invenção refere-se a métodos para produção de um material de planta transgênica (por exemplo, uma planta transgênica) compreendendo um peptídeo de trânsito de cloroplasto, bem como materiais de planta produzidos através de tais métodos, e produtos de consumo de planta produzidos a partir dos mesmos.
BR112014025946A 2012-04-17 2013-04-17 Molécula de ácido nucleico e proteína quimérica compreendendo um peptídeo de trânsito de cloroplasto BR112014025946B8 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201261625222P 2012-04-17 2012-04-17
US61/625,222 2012-04-17
PCT/US2013/036980 WO2013158766A1 (en) 2012-04-17 2013-04-17 Synthetic brassica-derived chloroplast transit peptides

Publications (3)

Publication Number Publication Date
BR112014025946A2 BR112014025946A2 (pt) 2017-07-11
BR112014025946B1 BR112014025946B1 (pt) 2022-09-13
BR112014025946B8 true BR112014025946B8 (pt) 2023-05-16

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BR112014025946A BR112014025946B8 (pt) 2012-04-17 2013-04-17 Molécula de ácido nucleico e proteína quimérica compreendendo um peptídeo de trânsito de cloroplasto

Country Status (19)

Country Link
EP (1) EP2846623B1 (pt)
JP (2) JP6652383B2 (pt)
KR (1) KR102061107B1 (pt)
CN (1) CN104394686B (pt)
AP (1) AP2014008051A0 (pt)
AU (2) AU2013205557B2 (pt)
BR (1) BR112014025946B8 (pt)
CA (1) CA2870538C (pt)
CL (2) CL2014002774A1 (pt)
ES (1) ES2738585T3 (pt)
IL (1) IL235201B (pt)
IN (1) IN2014DN09468A (pt)
MX (2) MX346574B (pt)
NZ (1) NZ701694A (pt)
PH (1) PH12014502316A1 (pt)
RU (1) RU2636035C2 (pt)
UA (1) UA116983C2 (pt)
WO (1) WO2013158766A1 (pt)
ZA (1) ZA201408351B (pt)

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US20170233758A1 (en) * 2016-02-15 2017-08-17 Daniyal Jawed Qureshi Herbicide tolerant triple gene insecticidal cotton and other plants
CN109475100A (zh) * 2016-07-14 2019-03-15 瑞克斯旺种苗集团公司 产生具有新型颜色的种子的茄子
CN109387580A (zh) * 2017-08-09 2019-02-26 江苏省农业科学院 一种监测甜玉米汁热加工中玉米黄质含量变化的方法
CN114085278B (zh) * 2022-01-20 2022-04-26 北京市农林科学院 Lsa25711转运肽在叶绿体遗传转化中的应用

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IN2014DN09468A (pt) 2015-07-17
EP2846623A1 (en) 2015-03-18
NZ701694A (en) 2016-09-30
CN104394686B (zh) 2018-02-06
ZA201408351B (en) 2016-09-28
AU2013205557B2 (en) 2016-04-21
WO2013158766A1 (en) 2013-10-24
CL2014002774A1 (es) 2015-04-10
AP2014008051A0 (en) 2014-11-30
CN104394686A (zh) 2015-03-04
BR112014025946A2 (pt) 2017-07-11
IL235201A0 (en) 2014-12-31
UA116983C2 (uk) 2018-06-11
BR112014025946B1 (pt) 2022-09-13
CA2870538C (en) 2021-08-03
EP2846623A4 (en) 2015-12-16
WO2013158766A9 (en) 2014-02-27
CL2018000837A1 (es) 2018-07-06
EP2846623B1 (en) 2019-06-19
IL235201B (en) 2019-11-28
MX2014012629A (es) 2015-05-12
JP2015514426A (ja) 2015-05-21
AU2013205557A1 (en) 2013-10-31
JP2020018305A (ja) 2020-02-06
KR102061107B1 (ko) 2019-12-31
PH12014502316A1 (en) 2015-01-12
KR20150008120A (ko) 2015-01-21
RU2636035C2 (ru) 2017-11-17
CA2870538A1 (en) 2013-10-24
JP6652383B2 (ja) 2020-02-19
AU2016206393B2 (en) 2017-12-07
ES2738585T3 (es) 2020-01-23
RU2014145864A (ru) 2016-06-10
MX346574B (es) 2017-03-24
AU2016206393A1 (en) 2016-08-11
MX355199B (es) 2018-04-09

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