BR112012015005A2 - Proteínas cry inseticidas cry1ca modificadas - Google Patents

Proteínas cry inseticidas cry1ca modificadas

Info

Publication number
BR112012015005A2
BR112012015005A2 BRBR112012015005-7A BR112012015005A BR112012015005A2 BR 112012015005 A2 BR112012015005 A2 BR 112012015005A2 BR 112012015005 A BR112012015005 A BR 112012015005A BR 112012015005 A2 BR112012015005 A2 BR 112012015005A2
Authority
BR
Brazil
Prior art keywords
dig
proteins
modified
cry proteins
insecticidal cry
Prior art date
Application number
BRBR112012015005-7A
Other languages
English (en)
Inventor
Thomas Meade
Kenneth Narva
Timothy Hey
Ignacio Larrinua
Aaron T Woosley
Stephanie L Burton
Original Assignee
Dow Agrosciences Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dow Agrosciences Llc filed Critical Dow Agrosciences Llc
Publication of BR112012015005A2 publication Critical patent/BR112012015005A2/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/32—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
    • C07K14/325—Bacillus thuringiensis crystal peptides, i.e. delta-endotoxins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8286—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for insect resistance
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Plant Pathology (AREA)
  • Pest Control & Pesticides (AREA)
  • Insects & Arthropods (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Agronomy & Crop Science (AREA)
  • Toxicology (AREA)
  • Dentistry (AREA)
  • Environmental Sciences (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

Proteínas cry inseticidas cry1ca modificadas. A presente invenção refere-se a proteínas inseticidas cry1ca de b.t. Modificadas, icnluindo as proteínas designadas aqui como dig-109 e dig-152, bem como variantes de dig-109 e dig-152, ácidos nucleicos que codificam essas proteínas, métodos de controle de pestes usando as proteínas, métodos de produção das proteínas em células hospedeiras transgênicas, e plantas transgênicas que produzem as proteínas. As proteínas dig-109e dig-152 compreendem peptídeos quiméricos compostos de um segmento de toxina central cry1ca de b.t. E um segmento de pró-toxina cry1ab. Variantes inseticidamente ativas das proteínas dig-109 e dig-152 são também descritas.
BRBR112012015005-7A 2009-12-16 2010-12-16 Proteínas cry inseticidas cry1ca modificadas BR112012015005A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US28427509P 2009-12-16 2009-12-16
PCT/US2010/060826 WO2011084627A2 (en) 2009-12-16 2010-12-16 Modified cry1ca insecticidal cry proteins

Publications (1)

Publication Number Publication Date
BR112012015005A2 true BR112012015005A2 (pt) 2015-09-01

Family

ID=44306062

Family Applications (1)

Application Number Title Priority Date Filing Date
BRBR112012015005-7A BR112012015005A2 (pt) 2009-12-16 2010-12-16 Proteínas cry inseticidas cry1ca modificadas

Country Status (18)

Country Link
US (1) US9284573B2 (pt)
EP (1) EP2512224A4 (pt)
JP (1) JP2013514772A (pt)
KR (1) KR101854478B1 (pt)
CN (1) CN102762096A (pt)
AR (1) AR079502A1 (pt)
AU (1) AU2010339916B2 (pt)
BR (1) BR112012015005A2 (pt)
CA (1) CA2782554A1 (pt)
CL (1) CL2012001631A1 (pt)
CO (1) CO6592053A2 (pt)
IL (1) IL220335A (pt)
MX (1) MX345224B (pt)
NZ (1) NZ601094A (pt)
RU (1) RU2012130019A (pt)
UA (1) UA112286C2 (pt)
WO (1) WO2011084627A2 (pt)
ZA (1) ZA201204918B (pt)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AR092905A1 (es) * 2012-10-05 2015-05-06 Dow Agrosciences Llc Uso de cry1ea en combinaciones para el control del insecto resistente gusano del ejercito del otoño
CN103145814B (zh) * 2013-02-25 2014-07-09 北京大北农科技集团股份有限公司 杀虫蛋白质、其编码基因及用途
CN117903266A (zh) * 2014-02-07 2024-04-19 先锋国际良种公司 杀昆虫蛋白及其使用方法
TW201542093A (zh) * 2014-03-21 2015-11-16 艾格里遺傳學股份有限公司 用於控制玉米穗蟲之Ｃｒｙ１Ｄ
US9738705B2 (en) * 2014-04-29 2017-08-22 Dow Agrosciences Llc Monoclonal antibodies specific for Cry1Ca and related detection methods
EP3207144B1 (en) * 2014-10-16 2019-05-01 Monsanto Technology LLC Lepidopteran-active cry1da1 amino acid sequence variant proteins
KR20170099907A (ko) * 2014-12-30 2017-09-01 다우 아그로사이언시즈 엘엘씨 곤충 해충의 방제에 유용한 변형된 Cry1Ca 독소
WO2016186986A1 (en) * 2015-05-19 2016-11-24 Pioneer Hi Bred International Inc Insecticidal proteins and methods for their use
US10412369B2 (en) 2015-07-31 2019-09-10 Dell Products, Lp Method and apparatus for compensating for camera error in a multi-camera stereo camera system
US11129906B1 (en) 2016-12-07 2021-09-28 David Gordon Bermudes Chimeric protein toxins for expression by therapeutic bacteria
CN109781993B (zh) * 2018-12-27 2021-04-06 中国农业科学院生物技术研究所 一种定量检测抗虫蛋白Cry1C的酶联免疫试剂盒
EP3927168A4 (en) * 2019-02-20 2023-02-08 Syngenta Crop Protection AG ENGINEERED PESTICIDE PROTEINS AND METHODS TO CONTROL PLANT PESTS
CN111100208A (zh) * 2020-01-16 2020-05-05 黑龙江大鹏农业有限公司 一种人工合成的抗虫蛋白mCry1Ia2及其制备方法和应用
CN111574599B (zh) * 2020-05-18 2022-10-28 福建农林大学 一种解决杀虫毒素被昆虫肠道消化酶过度酶解的毒素改造方法

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5380831A (en) 1986-04-04 1995-01-10 Mycogen Plant Science, Inc. Synthetic insecticidal crystal protein gene
US6078854A (en) * 1995-06-07 2000-06-20 Automotive Technologies International, Inc. Apparatus and method for adjusting a vehicle component
GB9318207D0 (en) 1993-09-02 1993-10-20 Sandoz Ltd Improvements in or relating to organic compounds
US5593881A (en) * 1994-05-06 1997-01-14 Mycogen Corporation Bacillus thuringiensis delta-endotoxin
US6110668A (en) 1996-10-07 2000-08-29 Max-Planck-Gesellschaft Zur Forderung Der Wissenschaften E.V. Gene synthesis method
US6017534A (en) 1996-11-20 2000-01-25 Ecogen, Inc. Hybrid Bacillus thuringiensis δ-endotoxins with novel broad-spectrum insecticidal activity
US5942664A (en) * 1996-11-27 1999-08-24 Ecogen, Inc. Bacillus thuringiensis Cry1C compositions toxic to lepidopteran insects and methods for making Cry1C mutants
US6218188B1 (en) * 1997-11-12 2001-04-17 Mycogen Corporation Plant-optimized genes encoding pesticidal toxins
EP1676922B1 (en) * 1997-11-12 2008-09-17 Mycogen Corporation Plant-optimized genes encoding pesticidal toxins
FR2795739B1 (fr) 1999-07-01 2003-08-15 Agronomique Inst Nat Rech Gene synthetique cryic et plantes transgeniques exprimant ledit gene
WO2002015701A2 (en) * 2000-08-25 2002-02-28 Syngenta Participations Ag Bacillus thuringiensis crystal protein hybrids
US7053266B2 (en) 2002-03-27 2006-05-30 Council Of Scientfic And Industrial Research Chimeric cry1E δendotoxin and methods of controlling insects
EP1893638B1 (en) 2005-06-08 2011-08-10 Pioneer-Hi-Bred International, Inc. Insect-specific protease recognition sequences

Also Published As

Publication number Publication date
CO6592053A2 (es) 2013-01-02
CA2782554A1 (en) 2011-07-14
KR101854478B1 (ko) 2018-05-03
EP2512224A2 (en) 2012-10-24
MX2012007124A (es) 2012-11-12
CN102762096A (zh) 2012-10-31
JP2013514772A (ja) 2013-05-02
RU2012130019A (ru) 2014-01-27
AR079502A1 (es) 2012-02-01
CL2012001631A1 (es) 2013-01-25
WO2011084627A3 (en) 2011-12-22
AU2010339916B2 (en) 2016-04-21
IL220335A (en) 2017-01-31
WO2011084627A2 (en) 2011-07-14
MX345224B (es) 2017-01-20
EP2512224A4 (en) 2013-04-10
US9284573B2 (en) 2016-03-15
US20130025006A1 (en) 2013-01-24
ZA201204918B (en) 2013-02-27
AU2010339916A1 (en) 2012-07-12
NZ601094A (en) 2014-10-31
KR20120101546A (ko) 2012-09-13
UA112286C2 (uk) 2016-08-25

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Legal Events

Date Code Title Description
B07A Application suspended after technical examination (opinion) [chapter 7.1 patent gazette]
B09B Patent application refused [chapter 9.2 patent gazette]
B12B Appeal against refusal [chapter 12.2 patent gazette]
B08F Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette]

Free format text: REFERENTE A 10A ANUIDADE.

B08K Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette]

Free format text: EM VIRTUDE DO ARQUIVAMENTO PUBLICADO NA RPI 2610 DE 12-01-2021 E CONSIDERANDO AUSENCIA DE MANIFESTACAO DENTRO DOS PRAZOS LEGAIS, INFORMO QUE CABE SER MANTIDO O ARQUIVAMENTO DO PEDIDO DE PATENTE, CONFORME O DISPOSTO NO ARTIGO 12, DA RESOLUCAO 113/2013.