BR112014025519B1 - Escherichia coli recombinante e método para produção de uma amida graxa - Google Patents

Escherichia coli recombinante e método para produção de uma amida graxa Download PDF

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Publication number
BR112014025519B1
BR112014025519B1 BR112014025519-9A BR112014025519A BR112014025519B1 BR 112014025519 B1 BR112014025519 B1 BR 112014025519B1 BR 112014025519 A BR112014025519 A BR 112014025519A BR 112014025519 B1 BR112014025519 B1 BR 112014025519B1
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Brazil
Prior art keywords
fatty
polypeptide
coli
less
acyl
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BR112014025519-9A
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English (en)
Portuguese (pt)
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BR112014025519A2 (OSRAM
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Jason J. Lutes
Stephen delCardayre
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Genomatica, Inc
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Priority to BR122019025755-8A priority Critical patent/BR122019025755B1/pt
Publication of BR112014025519A2 publication Critical patent/BR112014025519A2/pt
Publication of BR112014025519B1 publication Critical patent/BR112014025519B1/pt

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/02—Amides, e.g. chloramphenicol or polyamides; Imides or polyimides; Urethanes, i.e. compounds comprising N-C=O structural element or polyurethanes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y203/00—Acyltransferases (2.3)
    • C12Y203/01—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C12Y203/01086—Fatty-acyl-CoA synthase (2.3.1.86)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/01—Carboxylic ester hydrolases (3.1.1)
    • C12Y301/01005—Lysophospholipase (3.1.1.5)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/02—Thioester hydrolases (3.1.2)
    • C12Y301/02014—Oleoyl-[acyl-carrier-protein] hydrolase (3.1.2.14), i.e. ACP-thioesterase
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/52—Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
BR112014025519-9A 2012-04-13 2013-03-12 Escherichia coli recombinante e método para produção de uma amida graxa BR112014025519B1 (pt)

Priority Applications (1)

Application Number Priority Date Filing Date Title
BR122019025755-8A BR122019025755B1 (pt) 2012-04-13 2013-03-12 Escherichia coli recombinante e método para produção de uma amida graxa

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201261623711P 2012-04-13 2012-04-13
US61/623,711 2012-04-13
PCT/US2013/030502 WO2013154721A1 (en) 2012-04-13 2013-03-12 Microbial production of alkanolamides and amidoamines and uses thereof

Publications (2)

Publication Number Publication Date
BR112014025519A2 BR112014025519A2 (OSRAM) 2017-08-15
BR112014025519B1 true BR112014025519B1 (pt) 2022-01-11

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ID=47913634

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Application Number Title Priority Date Filing Date
BR112014025519-9A BR112014025519B1 (pt) 2012-04-13 2013-03-12 Escherichia coli recombinante e método para produção de uma amida graxa

Country Status (13)

Country Link
US (3) US9683247B2 (OSRAM)
EP (2) EP4170026A1 (OSRAM)
JP (1) JP6251241B2 (OSRAM)
KR (3) KR102278343B1 (OSRAM)
CN (3) CN104379735A (OSRAM)
AU (1) AU2013246405B2 (OSRAM)
BR (1) BR112014025519B1 (OSRAM)
CA (2) CA2870257C (OSRAM)
CO (1) CO7200250A2 (OSRAM)
IN (1) IN2014DN09217A (OSRAM)
MX (2) MX354883B (OSRAM)
MY (1) MY167227A (OSRAM)
WO (1) WO2013154721A1 (OSRAM)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104379735A (zh) 2012-04-13 2015-02-25 Reg生命科学有限责任公司 烷醇酰胺及酰胺胺的微生物生产及其用途
EP3647430B1 (en) * 2013-12-05 2022-10-26 Genomatica, Inc. Microbial production of fatty amines
CN105884643B (zh) * 2016-05-12 2018-03-27 浙江海正药业股份有限公司 一种不饱和脂肪酸酰胺类化合物及其制备方法和用途
CN107022513A (zh) * 2017-03-17 2017-08-08 河北美邦工程科技股份有限公司 一种重组大肠杆菌菌株生产长链二元酸的方法
CN109082387B (zh) * 2018-03-14 2022-03-18 重庆理工大学 一种可低温去除高氨氮的异养硝化-好氧反硝化复合菌剂及其应用
EP3844269A4 (en) 2018-08-31 2022-07-27 Genomatica, Inc. PRODUCTION OF NON-NATIVE MONO-UNSATURATED FATTY ACIDS IN BACTERIA
CA3130763A1 (en) 2019-02-25 2020-09-03 Ginkgo Bioworks, Inc. Biosynthesis of cannabinoids and cannabinoid precursors
US11441162B2 (en) * 2019-03-08 2022-09-13 Massachusetts Institute Of Technology Human gut microbiome-derived biosynthetic enzymes for production of fatty acid amides
CN110272839B (zh) * 2019-05-08 2020-10-30 江南大学 一株不动杆菌及其在生产手性3-环己烯-1-甲酸中的应用
GB202019175D0 (en) * 2020-12-04 2021-01-20 Univ Tartu Biosynthesis method
US11286467B1 (en) 2020-12-14 2022-03-29 United Arab Emirates University System and method for production of fatty acid ethanolamides
US20250207159A1 (en) 2022-03-16 2025-06-26 Genomatica, Inc. Microorganisms And Methods For Production Of Fatty Acid Derivatives With Reduced Levels Of Byproducts
WO2023178193A1 (en) 2022-03-16 2023-09-21 Genomatica, Inc. Acyl-acp thioesterase variants and uses thereof

Family Cites Families (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5028539A (en) 1988-08-31 1991-07-02 The University Of Florida Ethanol production using engineered mutant E. coli
US5482846A (en) 1988-08-31 1996-01-09 University Of Florida Ethanol production in Gram-positive microbes
US5000000A (en) 1988-08-31 1991-03-19 University Of Florida Ethanol production by Escherichia coli strains co-expressing Zymomonas PDC and ADH genes
US5424202A (en) 1988-08-31 1995-06-13 The University Of Florida Ethanol production by recombinant hosts
US5602030A (en) 1994-03-28 1997-02-11 University Of Florida Research Foundation Recombinant glucose uptake system
US6428767B1 (en) 1995-05-12 2002-08-06 E. I. Du Pont De Nemours And Company Method for identifying the source of carbon in 1,3-propanediol
US5576282A (en) 1995-09-11 1996-11-19 The Procter & Gamble Company Color-safe bleach boosters, compositions and laundry methods employing same
MA24136A1 (fr) 1996-04-16 1997-12-31 Procter & Gamble Fabrication d'agents de surface .
US6306812B1 (en) 1997-03-07 2001-10-23 Procter & Gamble Company, The Bleach compositions containing metal bleach catalyst, and bleach activators and/or organic percarboxylic acids
DE19731881C1 (de) 1997-07-24 1998-10-22 Henkel Kgaa Verwendung von Elektrolytgemischen als Sequestriermittel
JP4817495B2 (ja) 1998-05-29 2011-11-16 ニューロサイエンシーズ・リサーチ・ファウンデーション・インコーポレーテッド 外因性カンナビノイドでの痛みの制御
WO2008100251A1 (en) 2007-02-13 2008-08-21 Ls9, Inc. Modified microorganism uses therefor
US20100242345A1 (en) 2006-05-19 2010-09-30 LS9, Inc Production of fatty acids & derivatives thereof
CA2650773C (en) 2006-05-19 2014-12-02 Jay D. Keasling Microorganisms transformed with acetyl-coa carboxylase and wax synthase for production of fatty acid derivatives
CN101490241A (zh) * 2006-05-19 2009-07-22 Ls9公司 脂肪酸及其衍生物的制备
BRPI0809345A2 (pt) 2007-03-28 2014-10-07 Ls9 Inc Células recombinantes, composições produzidas por estas, métodos para produzir derivados de ácidos graxos em uma célula recombinante, para aumentar a produção de derivados de ácidos graxos em uma célula recombinante, construto recombinante, vetores, métodos para aumentar a produção de derivados de ácidos graxos em uma célula hospedeira de produção, para produzir derivados de ácidos graxos, derivados de ácidos graxos, composições de biocombustível.
US8048654B2 (en) * 2010-06-09 2011-11-01 Joule Unlimited Technologies, Inc. Methods and compositions for the recombinant biosynthesis of fatty acids and esters
CA3038491A1 (en) 2008-10-07 2010-04-15 REG Life Sciences, LLC Bacterial host cells engineered to express a carboxylic acid reductase and a thioesterase
CA2740037C (en) 2008-10-28 2019-06-18 Ls9, Inc. Methods for producing a fatty alcohol in a host cell
EP2382285B1 (en) 2008-12-23 2019-05-01 REG Life Sciences, LLC Methods and compositions related to thioesterase enzymes
CA2758298A1 (en) 2009-04-10 2010-10-14 Ls9, Inc. Production of commercial biodiesel from genetically modified microorganisms
CA3056664A1 (en) 2009-09-25 2011-03-31 Alfred Gaertner Production of fatty acid derivatives in recombinant bacterial cells expressing an ester synthase variant
US8859259B2 (en) 2010-02-14 2014-10-14 Ls9, Inc. Surfactant and cleaning compositions comprising microbially produced branched fatty alcohols
CN104379735A (zh) 2012-04-13 2015-02-25 Reg生命科学有限责任公司 烷醇酰胺及酰胺胺的微生物生产及其用途

Also Published As

Publication number Publication date
JP6251241B2 (ja) 2017-12-20
MX354883B (es) 2018-03-23
CA2870257A1 (en) 2013-10-17
CA2870257C (en) 2020-07-28
US20200232001A1 (en) 2020-07-23
AU2013246405B2 (en) 2018-08-02
AU2013246405A1 (en) 2014-10-30
KR20150031230A (ko) 2015-03-23
KR102190262B1 (ko) 2020-12-14
US10392637B2 (en) 2019-08-27
US9683247B2 (en) 2017-06-20
CN104379735A (zh) 2015-02-25
KR20200105535A (ko) 2020-09-07
CN108949660A (zh) 2018-12-07
US20150079643A1 (en) 2015-03-19
KR20210091353A (ko) 2021-07-21
JP2015516808A (ja) 2015-06-18
CA3081178C (en) 2023-02-21
US10975402B2 (en) 2021-04-13
IN2014DN09217A (OSRAM) 2015-07-10
EP4170026A1 (en) 2023-04-26
MX391947B (es) 2025-03-21
CN112522170A (zh) 2021-03-19
CA3081178A1 (en) 2013-10-17
WO2013154721A1 (en) 2013-10-17
EP2836593A1 (en) 2015-02-18
BR112014025519A2 (OSRAM) 2017-08-15
MY167227A (en) 2018-08-14
CO7200250A2 (es) 2015-02-27
US20170292120A1 (en) 2017-10-12
KR102278343B1 (ko) 2021-07-16
MX2014012350A (es) 2015-06-04

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B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B06U Preliminary requirement: requests with searches performed by other patent offices: procedure suspended [chapter 6.21 patent gazette]
B25A Requested transfer of rights approved

Owner name: GENOMATICA, INC. (US)

B06A Patent application procedure suspended [chapter 6.1 patent gazette]
B350 Update of information on the portal [chapter 15.35 patent gazette]
B350 Update of information on the portal [chapter 15.35 patent gazette]
B09A Decision: intention to grant [chapter 9.1 patent gazette]
B16A Patent or certificate of addition of invention granted [chapter 16.1 patent gazette]

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B21F Lapse acc. art. 78, item iv - on non-payment of the annual fees in time

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B24J Lapse because of non-payment of annual fees (definitively: art 78 iv lpi, resolution 113/2013 art. 12)

Free format text: EM VIRTUDE DA EXTINCAO PUBLICADA NA RPI 2765 DE 02-01-2024 E CONSIDERANDO AUSENCIA DE MANIFESTACAO DENTRO DOS PRAZOS LEGAIS, INFORMO QUE CABE SER MANTIDA A EXTINCAO DA PATENTE E SEUS CERTIFICADOS, CONFORME O DISPOSTO NO ARTIGO 12, DA RESOLUCAO 113/2013.