BR112013001270A2 - celulossoma artificial e o uso do mesmo para a degradação enzimática de substratos resilentes. - Google Patents

celulossoma artificial e o uso do mesmo para a degradação enzimática de substratos resilentes.

Info

Publication number
BR112013001270A2
BR112013001270A2 BR112013001270A BR112013001270A BR112013001270A2 BR 112013001270 A2 BR112013001270 A2 BR 112013001270A2 BR 112013001270 A BR112013001270 A BR 112013001270A BR 112013001270 A BR112013001270 A BR 112013001270A BR 112013001270 A2 BR112013001270 A2 BR 112013001270A2
Authority
BR
Brazil
Prior art keywords
enzymatic degradation
resilient substrates
resilient
substrates
artificial cellulosome
Prior art date
Application number
BR112013001270A
Other languages
English (en)
Inventor
Daniel Hornburg
Daniela Köck
Jan Krauss
Louis-Philipp Schulte
Vladimir V Zverlov
Wolfgang H Schwarz
Original Assignee
Wolfgang H Schwarz
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Wolfgang H Schwarz filed Critical Wolfgang H Schwarz
Publication of BR112013001270A2 publication Critical patent/BR112013001270A2/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/96—Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/244—Endo-1,3(4)-beta-glucanase (3.2.1.6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2445—Beta-glucosidase (3.2.1.21)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01004—Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01006—Endo-1,3(4)-beta-glucanase (3.2.1.6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01021—Beta-glucosidase (3.2.1.21)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Materials For Medical Uses (AREA)
  • Prostheses (AREA)

Abstract

celulossoma artificial e o uso do mesmo para a degradação enzimática de substratos resilientes - a presente invenção refere-se a um celulossoma artificial produzido in vitro para a degradação enzimática de substratos resilientes. em particular, a presente invenção fornece um complexo com uma atividade aumentada em substratos resilientes, tal como, a celulose cristalina. o complexo formado in vitro compreende um arcabouço de cadeia principal tendo ao menos quatro sítios de ligação capazes de ligar os componentes enzimáticos, em que ao menos dois dos sítios de ligação têm essencialmente a mesma especificidade de ligação; e ao menos três diferentes componentes enzimáticos limitados aleatoriamente a ao menos quatro sítios de ligação. um método para preparar o complexo e seus usos para a degradação enzimática de substratos resilientes também são fornecidos.
BR112013001270A 2010-07-20 2011-07-19 celulossoma artificial e o uso do mesmo para a degradação enzimática de substratos resilentes. BR112013001270A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP10007525A EP2410061A1 (en) 2010-07-20 2010-07-20 Artificial cellulosome and the use of the same for enzymatic breakdown of resilient substrates
PCT/EP2011/003617 WO2012010295A1 (en) 2010-07-20 2011-07-19 Artificial cellulosome and the use of the same for enzymatic breakdown of resilient substrates

Publications (1)

Publication Number Publication Date
BR112013001270A2 true BR112013001270A2 (pt) 2016-05-17

Family

ID=42751706

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112013001270A BR112013001270A2 (pt) 2010-07-20 2011-07-19 celulossoma artificial e o uso do mesmo para a degradação enzimática de substratos resilentes.

Country Status (8)

Country Link
US (1) US20130189745A1 (pt)
EP (2) EP2410061A1 (pt)
JP (1) JP5909229B2 (pt)
CN (1) CN103025879B (pt)
BR (1) BR112013001270A2 (pt)
CA (1) CA2805934A1 (pt)
RU (1) RU2580047C2 (pt)
WO (1) WO2012010295A1 (pt)

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MY175253A (en) * 2012-03-10 2020-06-17 Japan International Res Center For Agricultural Sciences Method for producing glucose
JP5935132B2 (ja) * 2012-03-24 2016-06-15 国立研究開発法人国際農林水産業研究センター 蛋白質の整列方法
US8993276B2 (en) * 2012-07-13 2015-03-31 Alliance For Sustainable Energy, Llc Chimeric enzymes with improved cellulase activities
GB201409047D0 (en) * 2014-05-21 2014-07-02 Cellucomp Ltd Cellulose microfibrils
EP3234162B1 (en) 2014-12-16 2019-10-23 Newpek S.A. De C.V. Enzymatic methods for isobutanol production
EP3297442A4 (en) 2015-05-18 2018-11-07 Zymtronix, LLC Magnetically immobilized microbiocidal enzymes
JP6445934B2 (ja) * 2015-06-08 2018-12-26 地方独立行政法人東京都立産業技術研究センター 廃棄汚泥の減少方法
CN108140848B (zh) * 2015-07-15 2022-02-01 齐姆特罗尼克斯公司 自动化生物纳米催化剂生产
US10907140B2 (en) 2015-10-15 2021-02-02 Technische Universitaet Muenchen Mutant beta-glucosidase variants with increased thermostability
CA3006972A1 (en) 2015-12-09 2017-06-15 The Regents Of The University Of California Novel proteins from anaerobic fungi and uses thereof
US10398745B2 (en) 2016-01-26 2019-09-03 Fujifilm Corporation Agent for reducing the number of intestinal bacteria, food, and pharmaceutical product
US11692207B2 (en) 2016-05-05 2023-07-04 Newpek S.A. De C.V. Enzymatic methods for butanol production
US20190174746A1 (en) 2016-08-13 2019-06-13 Zymtronix Catalytic Systems, Inc. Magnetically immobilized biocidal enzymes and biocidal chemicals
IL247569A0 (en) 2016-08-30 2016-11-30 Yeda Res & Dev An enzymatic complex for the breakdown of lignocellulosic material
CN106884010A (zh) * 2017-01-14 2017-06-23 北京化工大学 一种纳米磁球定向比例可控固定多酶的方法
CN108866025B (zh) * 2017-05-10 2021-04-13 中国科学院青岛生物能源与过程研究所 一种纤维素酶制剂及其应用
CN110527640B (zh) * 2018-05-25 2021-01-26 中国农业科学院北京畜牧兽医研究所 一种基于纤维素化磁性纳米颗粒富集和分离厌氧纤维降解菌的方法
AR124748A1 (es) 2018-07-06 2023-05-03 Moselle Tech Llc Métodos y composiciones para la recuperación de litio de soluciones líquidas con nanopartículas
CN110563852B (zh) * 2019-09-27 2021-08-03 北京理工大学 一种高效简便的酶固定化的方法
CN111850007B (zh) * 2020-07-27 2022-03-29 齐鲁工业大学 一种适用于低钙离子浓度的纤维小体对接蛋白组合突变体36864及应用
CN114410505B (zh) * 2021-12-17 2023-10-24 苏州聚维元创生物科技有限公司 热纤梭菌及其在木质纤维素水解中的应用
CN114836404B (zh) * 2022-06-09 2023-04-25 陕西理工大学 一种复合耐热纤维素酶及其制备方法和应用

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Also Published As

Publication number Publication date
US20130189745A1 (en) 2013-07-25
CN103025879A (zh) 2013-04-03
EP2410061A1 (en) 2012-01-25
CN103025879B (zh) 2016-08-03
RU2580047C2 (ru) 2016-04-10
WO2012010295A1 (en) 2012-01-26
EP2596108A1 (en) 2013-05-29
RU2013107377A (ru) 2014-08-27
CA2805934A1 (en) 2012-01-26
JP2013530717A (ja) 2013-08-01
JP5909229B2 (ja) 2016-04-26

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Legal Events

Date Code Title Description
B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B08F Application fees: application dismissed [chapter 8.6 patent gazette]
B08K Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette]

Free format text: EM VIRTUDE DO ARQUIVAMENTO PUBLICADO NA RPI 2471 DE 15-05-2018 E CONSIDERANDO AUSENCIA DE MANIFESTACAO DENTRO DOS PRAZOS LEGAIS, INFORMO QUE CABE SER MANTIDO O ARQUIVAMENTO DO PEDIDO DE PATENTE, CONFORME O DISPOSTO NO ARTIGO 12, DA RESOLUCAO 113/2013.