BR112012020277A2 - "enzimas celulase otimizadas". - Google Patents

"enzimas celulase otimizadas".

Info

Publication number
BR112012020277A2
BR112012020277A2 BR112012020277A BR112012020277A BR112012020277A2 BR 112012020277 A2 BR112012020277 A2 BR 112012020277A2 BR 112012020277 A BR112012020277 A BR 112012020277A BR 112012020277 A BR112012020277 A BR 112012020277A BR 112012020277 A2 BR112012020277 A2 BR 112012020277A2
Authority
BR
Brazil
Prior art keywords
enzymes
optimized
cellulase enzymes
disclosed
cellulose
Prior art date
Application number
BR112012020277A
Other languages
English (en)
Inventor
Andre Koltermann
Andreas Kohl
Christoph Reisinger
Dominik Schlosser
Isabel Unterstrasser
Jan Carsten Pieck
Jochen Gerlach
Jörg Claren
Lutz RÖCHER
Markus Rarbach
Thomas Brück
Ulrich Kettling
Original Assignee
Süd Chemie Ip Gmbh & Co Kg
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Süd Chemie Ip Gmbh & Co Kg filed Critical Süd Chemie Ip Gmbh & Co Kg
Publication of BR112012020277A2 publication Critical patent/BR112012020277A2/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01091—Cellulose 1,4-beta-cellobiosidase (3.2.1.91)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Abstract

enzimas celulase otimizadas. a invenção revela enzimas celulase com propriedades otimizadas para o processamento de substratos contendo celulose e lignocelulose. são reveladas, em particular, enzimas celobiohidrolase com características preferenciais. a presente invenção proporciona variantes de tais enzimas por fusão, inserção, deleção e/ou substituição. as enzimas variantes têm termoestabilidade reforçada, estabilidade proteolítica, atividade específica e/ou estabilidade em ph extremo. são divulgadas moléculas de ácido nucleico que codificam as referidas enzimas, uma composição compreendendo as referidas enzimas, um método para a preparação, e a utilização para o processamento de celulose e/ou para a produção de biocombustíveis.
BR112012020277A 2010-02-11 2011-02-11 "enzimas celulase otimizadas". BR112012020277A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP10153355.2A EP2357227B1 (en) 2010-02-11 2010-02-11 Optimized cellulase enzymes
PCT/EP2011/052023 WO2011098551A2 (en) 2010-02-11 2011-02-11 Optimized cellulase enzymes

Publications (1)

Publication Number Publication Date
BR112012020277A2 true BR112012020277A2 (pt) 2015-09-15

Family

ID=42027636

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112012020277A BR112012020277A2 (pt) 2010-02-11 2011-02-11 "enzimas celulase otimizadas".

Country Status (14)

Country Link
US (1) US9458443B2 (pt)
EP (2) EP2357227B1 (pt)
CN (1) CN102844430A (pt)
AU (1) AU2011214298B2 (pt)
BR (1) BR112012020277A2 (pt)
CA (1) CA2789423C (pt)
DK (2) DK2357227T3 (pt)
ES (2) ES2549328T3 (pt)
HR (2) HRP20151043T1 (pt)
MX (1) MX344963B (pt)
PL (2) PL2357227T3 (pt)
RS (1) RS57695B1 (pt)
SI (2) SI2357227T1 (pt)
WO (1) WO2011098551A2 (pt)

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CA2796189A1 (en) 2010-06-01 2011-12-08 California Institute Of Technology Stable, functional chimeric cellobiohydrolase class i enzymes
CA2811789A1 (en) 2010-10-06 2012-04-12 Bp Corporation North America Inc. Variant cbh i polypeptides with reduced product inhibition
US20140080182A1 (en) * 2011-03-31 2014-03-20 Novozymes, Inc. Cellulose Binding Domain Variants and Polynucleotides Encoding Same
CA2856083A1 (en) * 2011-11-15 2013-05-23 Novozymes, Inc. Polypeptides having cellobiohydrolase activity and polynucleotides encoding same
WO2013091577A1 (en) 2011-12-22 2013-06-27 Novozymes, Inc. Hybrid polypeptides having cellobiohydrolase activity and polynucleotides encoding same
MX2015007234A (es) 2012-12-12 2015-10-29 Danisco Us Inc Variantes de celobiohidrolasas.
US20150315560A1 (en) * 2012-12-12 2015-11-05 Danisco Us Inc. Variants of cellobiohydrolases
CN105164254B (zh) 2013-03-08 2019-06-28 诺维信公司 纤维二糖水解酶变体和编码它们的多核苷酸
CN107002056A (zh) 2014-09-05 2017-08-01 诺维信公司 碳水化合物结合模块变体以及编码它们的多核苷酸
DK3191587T3 (da) 2014-09-08 2019-10-14 Novozymes As Cellobiohydrolasevarianter og polynukleotider, som koder for dem
JP6378622B2 (ja) * 2014-12-04 2018-08-22 本田技研工業株式会社 高活性セロビオヒドロラーゼ
US10557127B2 (en) 2015-02-24 2020-02-11 Novozymes A/S Cellobiohydrolase variants and polynucleotides encoding same
WO2017177289A1 (en) 2016-04-15 2017-10-19 Vtt International Oy Process for conversion of biomass into fermentable sugars with integrated enzyme
CN109715182A (zh) * 2016-06-15 2019-05-03 科德克希思公司 工程化β-葡糖苷酶和葡糖基化方法
CN109337888B (zh) * 2018-11-12 2021-08-17 南开大学 纤维二糖水解酶突变体及其生产方法和应用
CN110373402B (zh) * 2019-08-13 2021-07-20 山东农业大学 一种提升耐热纤维素酶酶活力和热稳定性的方法
CN113186175B (zh) * 2021-06-04 2023-08-04 翌圣生物科技(上海)股份有限公司 突变型Taq DNA聚合酶、编码DNA序列、重组载体、重组表达细胞及其应用
CN114426961B (zh) * 2022-03-10 2024-01-26 安徽大学 一种β-葡萄糖苷酶突变体、其编码基因、表达菌株及其应用

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Also Published As

Publication number Publication date
WO2011098551A2 (en) 2011-08-18
CA2789423C (en) 2017-12-12
US20130123115A1 (en) 2013-05-16
EP2534245B1 (en) 2018-07-04
HRP20181587T1 (hr) 2018-12-14
AU2011214298B2 (en) 2014-11-06
CA2789423A1 (en) 2011-08-18
HRP20151043T1 (hr) 2015-11-06
PL2357227T3 (pl) 2015-12-31
EP2357227B1 (en) 2015-07-15
ES2549328T3 (es) 2015-10-27
DK2534245T3 (en) 2018-10-01
DK2357227T3 (en) 2015-09-14
PL2534245T3 (pl) 2019-02-28
ES2689939T3 (es) 2018-11-16
US9458443B2 (en) 2016-10-04
CN102844430A (zh) 2012-12-26
WO2011098551A3 (en) 2012-05-31
AU2011214298A1 (en) 2012-09-06
EP2357227A1 (en) 2011-08-17
SI2357227T1 (sl) 2015-11-30
RS57695B1 (sr) 2018-11-30
SI2534245T1 (sl) 2019-01-31
EP2534245A2 (en) 2012-12-19
MX344963B (es) 2017-01-11
MX2012009357A (es) 2012-12-17

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Owner name: CLARIANT PRODUKTE (DEUTSCHLAND) GMBH (DE)

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Free format text: EM VIRTUDE DO ARQUIVAMENTO PUBLICADO NA RPI 2761 DE 05-12-2023 E CONSIDERANDO AUSENCIA DE MANIFESTACAO DENTRO DOS PRAZOS LEGAIS, INFORMO QUE CABE SER MANTIDO O ARQUIVAMENTO DO PEDIDO DE PATENTE, CONFORME O DISPOSTO NO ARTIGO 12, DA RESOLUCAO 113/2013.