MX2012009357A - Enzimas celulasas optimizadas. - Google Patents

Enzimas celulasas optimizadas.

Info

Publication number
MX2012009357A
MX2012009357A MX2012009357A MX2012009357A MX2012009357A MX 2012009357 A MX2012009357 A MX 2012009357A MX 2012009357 A MX2012009357 A MX 2012009357A MX 2012009357 A MX2012009357 A MX 2012009357A MX 2012009357 A MX2012009357 A MX 2012009357A
Authority
MX
Mexico
Prior art keywords
enzymes
disclosed
cellulase enzymes
cellulose
processing
Prior art date
Application number
MX2012009357A
Other languages
English (en)
Other versions
MX344963B (es
Inventor
Ulrich Kettling
Andre Koltermann
Christoph Reisinger
Brueck Thomas
Jochen Gerlach
Isabel Unterstrasser
Lutz Roecher
Markus Rarbach
Joerg Claren
Andreas Kohl
Jan Carsten Pieck
Dominik Schlosser
Original Assignee
Sued Chemie Ip Gmbh & Co Kg
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sued Chemie Ip Gmbh & Co Kg filed Critical Sued Chemie Ip Gmbh & Co Kg
Publication of MX2012009357A publication Critical patent/MX2012009357A/es
Publication of MX344963B publication Critical patent/MX344963B/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01091—Cellulose 1,4-beta-cellobiosidase (3.2.1.91)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Abstract

La invención desvela enzimas celulasa con propiedades optimizadas para procesamiento de sustratos que contienen celulosa y lignocelulosa. En particular, se desvelan enzimas celobiohidrolasa con características preferidas. La presente invención proporciona variantes de fusión, inserción, deleción y/o sustitución de tales enzimas. Las variantes enzimáticas tienen termoestabilidad, estabilidad proteolítica, actividad específica y/o estabilidad potenciadas a pH extremo. Se desvelan moléculas de ácido nucleico que codifican dichas enzimas, una composición que comprende dichas enzimas, un procedimiento para preparación y el uso para procesamiento de celulosa y/o para la producción de biocarburantes.
MX2012009357A 2010-02-11 2011-02-11 Enzimas celulasas optimizadas. MX344963B (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP10153355.2A EP2357227B1 (en) 2010-02-11 2010-02-11 Optimized cellulase enzymes
PCT/EP2011/052023 WO2011098551A2 (en) 2010-02-11 2011-02-11 Optimized cellulase enzymes

Publications (2)

Publication Number Publication Date
MX2012009357A true MX2012009357A (es) 2012-12-17
MX344963B MX344963B (es) 2017-01-11

Family

ID=42027636

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2012009357A MX344963B (es) 2010-02-11 2011-02-11 Enzimas celulasas optimizadas.

Country Status (14)

Country Link
US (1) US9458443B2 (es)
EP (2) EP2357227B1 (es)
CN (1) CN102844430A (es)
AU (1) AU2011214298B2 (es)
BR (1) BR112012020277A2 (es)
CA (1) CA2789423C (es)
DK (2) DK2357227T3 (es)
ES (2) ES2549328T3 (es)
HR (2) HRP20151043T1 (es)
MX (1) MX344963B (es)
PL (2) PL2357227T3 (es)
RS (1) RS57695B1 (es)
SI (2) SI2357227T1 (es)
WO (1) WO2011098551A2 (es)

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EP2576626B1 (en) * 2010-06-01 2018-08-01 California Institute of Technology Stable, functional chimeric cellobiohydrolase class i enzymes
AR083355A1 (es) 2010-10-06 2013-02-21 Bp Corp North America Inc Polipeptidos variantes cbh i
WO2012135719A1 (en) * 2011-03-31 2012-10-04 Novozymes, Inc. Cellulose binding domain variants and polynucleotides encoding same
CA2856083A1 (en) * 2011-11-15 2013-05-23 Novozymes, Inc. Polypeptides having cellobiohydrolase activity and polynucleotides encoding same
DK2794664T3 (en) 2011-12-22 2017-08-21 Novozymes Inc HYBRID POLYPEPTIDES WITH CELLOBIO HYDROLASE ACTIVITY AND POLYNUCLEOTIDES CODING THEM
KR20150093729A (ko) * 2012-12-12 2015-08-18 다니스코 유에스 인크. 셀로바이오하이드롤라제의 변이체
CA2892786A1 (en) 2012-12-12 2014-06-19 Danisco Us Inc. Variants of cellobiohydrolases
US10221406B2 (en) 2013-03-08 2019-03-05 Novozymes, Inc. Cellobiohydrolase variants and polynucleotides encoding same
US11390898B2 (en) 2014-09-05 2022-07-19 Novozymes A/S Polypeptides having cellobiohydrolase activity and polynucleotides encoding same
EP3191587B1 (en) 2014-09-08 2019-07-17 Novozymes A/S Cellobiohydrolase variants and polynucleotides encoding same
JP6378622B2 (ja) * 2014-12-04 2018-08-22 本田技研工業株式会社 高活性セロビオヒドロラーゼ
EP3739045A3 (en) 2015-02-24 2021-03-10 Novozymes A/S Cellobiohydrolase variants and polynucleotides encoding same
WO2017177289A1 (en) 2016-04-15 2017-10-19 Vtt International Oy Process for conversion of biomass into fermentable sugars with integrated enzyme
US11299723B2 (en) 2016-06-15 2022-04-12 Codexis, Inc. Engineered beta-glucosidases and glucosylation methods
CN109337888B (zh) * 2018-11-12 2021-08-17 南开大学 纤维二糖水解酶突变体及其生产方法和应用
CN110373402B (zh) * 2019-08-13 2021-07-20 山东农业大学 一种提升耐热纤维素酶酶活力和热稳定性的方法
CN113186175B (zh) * 2021-06-04 2023-08-04 翌圣生物科技(上海)股份有限公司 突变型Taq DNA聚合酶、编码DNA序列、重组载体、重组表达细胞及其应用
CN114426961B (zh) * 2022-03-10 2024-01-26 安徽大学 一种β-葡萄糖苷酶突变体、其编码基因、表达菌株及其应用
CN117802072A (zh) * 2024-02-29 2024-04-02 北京挑战生物技术有限公司 木聚糖酶突变体及其应用

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EP1200574A4 (en) 1999-07-13 2004-03-17 Midwest Research Inst REDUCED GLYCOSYLATION VARIANTS OF CELLOBIOHYDROLASE: CBHIN45A, CBHIN270A AND CBHIN384A
US7375197B2 (en) 2002-01-14 2008-05-20 Midwest Research Institute Cellobiohydrolase I gene and improved variants
US8637293B2 (en) 1999-07-13 2014-01-28 Alliance For Sustainable Energy, Llc Cellobiohydrolase I enzymes
EP2298868B1 (en) 2001-06-26 2015-01-07 Novozymes A/S Polypeptides having cellobiohydrolase I activity and polynucleotides encoding same
DK2295559T3 (en) 2002-08-16 2015-12-14 Danisco Us Inc Hitherto UNKNOWN HYPROCREA JECORINA CBH1 CELLULASE VARIETY WITH INCREASED THERMAL STABILITY COMPREHENSIVE SUBSTITUTION OR DELETION AT POSITION T255
AU2003219956A1 (en) * 2003-02-27 2004-09-28 Midwest Research Institute Superactive cellulase formulation using cellobiohydrolase-1 from penicillium funiculosum
EP1606392A4 (en) 2003-03-21 2007-10-17 Genencor Int NEW CBH1 HOMOLOGO AND CBH1 CELLULASE VARIANTS
JP5427342B2 (ja) 2003-04-01 2014-02-26 ジェネンコー・インターナショナル・インク 変異体フミコーラ・グリセアｃｂｈ１．１
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Also Published As

Publication number Publication date
AU2011214298B2 (en) 2014-11-06
SI2534245T1 (sl) 2019-01-31
BR112012020277A2 (pt) 2015-09-15
US9458443B2 (en) 2016-10-04
HRP20181587T1 (hr) 2018-12-14
ES2549328T3 (es) 2015-10-27
EP2534245A2 (en) 2012-12-19
DK2357227T3 (en) 2015-09-14
CA2789423C (en) 2017-12-12
PL2357227T3 (pl) 2015-12-31
WO2011098551A2 (en) 2011-08-18
RS57695B1 (sr) 2018-11-30
DK2534245T3 (en) 2018-10-01
US20130123115A1 (en) 2013-05-16
CN102844430A (zh) 2012-12-26
WO2011098551A3 (en) 2012-05-31
CA2789423A1 (en) 2011-08-18
ES2689939T3 (es) 2018-11-16
MX344963B (es) 2017-01-11
SI2357227T1 (sl) 2015-11-30
AU2011214298A1 (en) 2012-09-06
EP2534245B1 (en) 2018-07-04
EP2357227A1 (en) 2011-08-17
PL2534245T3 (pl) 2019-02-28
HRP20151043T1 (hr) 2015-11-06
EP2357227B1 (en) 2015-07-15

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