BR112012031965A8 - uso de promotores induzíveis na produção de ácido glicólico - Google Patents

uso de promotores induzíveis na produção de ácido glicólico Download PDF

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Publication number
BR112012031965A8
BR112012031965A8 BR112012031965A BR112012031965A BR112012031965A8 BR 112012031965 A8 BR112012031965 A8 BR 112012031965A8 BR 112012031965 A BR112012031965 A BR 112012031965A BR 112012031965 A BR112012031965 A BR 112012031965A BR 112012031965 A8 BR112012031965 A8 BR 112012031965A8
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BR
Brazil
Prior art keywords
production
inducible promoters
acid
glycoric acid
glycoric
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BR112012031965A
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English (en)
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BR112012031965A2 (pt
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Soucaille Philippe
Figge Rainer
Dischert Wanda
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Metabolic Explorer Sa
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Application filed by Metabolic Explorer Sa filed Critical Metabolic Explorer Sa
Publication of BR112012031965A2 publication Critical patent/BR112012031965A2/pt
Publication of BR112012031965A8 publication Critical patent/BR112012031965A8/pt

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/42—Hydroxy-carboxylic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/635—Externally inducible repressor mediated regulation of gene expression, e.g. tetR inducible by tetracyline
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

uso de promotores induzíveis na produção de ácido glicórico - a presente invenção se refere ao uso de promotores induzíveis na produção de ácido glicórico através de fermentação. a presente invenção diz respeito a um método para a produção de ácido glicórico em um processo fermentativo que compreende as seguintes etapas: - cultivar um micro-organismo modificado em um meio de cultura apropriado que compreende uma fonte de carbono, - modular, no referido micro-organismo, a expressão de um gene alvo com um estímulo externo, e - recuperar ácido glicórico a partir do meio de cultura, - em que no referido micro-organismo modificado, a expressão de pelo menos um gene envolvido na produção de ácido glicórico está sob o controle de um promotor induzível heterólogo, cuja atividade é modulada com o referido estímulo externo. a invenção também diz respeito ao micro-organismo modificado no método de produção de ácido glicórico.
BR112012031965A 2010-06-15 2011-06-15 uso de promotores induzíveis na produção de ácido glicólico BR112012031965A8 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US35488710P 2010-06-15 2010-06-15
EP10305635 2010-06-15
PCT/EP2011/059884 WO2011157728A1 (en) 2010-06-15 2011-06-15 Use of inducible promoters in the production of glycolic acid

Publications (2)

Publication Number Publication Date
BR112012031965A2 BR112012031965A2 (pt) 2015-11-24
BR112012031965A8 true BR112012031965A8 (pt) 2020-05-19

Family

ID=42490551

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112012031965A BR112012031965A8 (pt) 2010-06-15 2011-06-15 uso de promotores induzíveis na produção de ácido glicólico

Country Status (8)

Country Link
US (1) US9121043B2 (pt)
EP (1) EP2582828B1 (pt)
JP (1) JP5882310B2 (pt)
KR (1) KR101824282B1 (pt)
CN (1) CN103221548B (pt)
BR (1) BR112012031965A8 (pt)
CA (1) CA2801823C (pt)
WO (1) WO2011157728A1 (pt)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3143148B1 (en) * 2014-05-12 2024-01-03 METabolic EXplorer New microorganism and method for the production of 1.2-propanediol based on nadph dependent acetol reductase and improved nadph supply
EP3365427B1 (en) 2015-10-23 2020-08-19 Metabolic Explorer Microorganism modified for the assimilation of levulinic acid
US10975420B2 (en) * 2015-12-18 2021-04-13 California Institute Of Technology Thermal bioswitches and related genetic circuits, vectors, cells, compositions, methods and systems
US10961499B2 (en) 2016-07-08 2021-03-30 Metabolic Explorer Method for the fermentative production of molecules of interest by microorganisms comprising genes coding sugar phosphotransferase system (PTS)
EP3354742A1 (en) 2017-01-26 2018-08-01 Metabolic Explorer Methods and microorganisms for the production of glycolic acid and/or glyoxylic acid
EP3692159A1 (en) 2017-10-02 2020-08-12 Metabolic Explorer Method for producing organic acid salts from fermentation broth
US11965001B2 (en) 2019-07-12 2024-04-23 California Institute Of Technology Modular dimerization thermoswitches and related monomers, dimers, constructs, dimeric complexes, vectors, cells, surfaces, devices compositions, methods and systems

Family Cites Families (15)

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Publication number Priority date Publication date Assignee Title
US5783716A (en) 1996-06-28 1998-07-21 Advanced Technology Materials, Inc. Platinum source compositions for chemical vapor deposition of platinum
WO1998011231A1 (en) 1996-09-10 1998-03-19 The Rockefeller University Highly regulable promoter for heterologous gene expression
EP2259534B1 (en) 1997-11-05 2014-08-06 Sony Deutschland Gmbh Synchronisation of digital communication systems
JP4267101B2 (ja) 1997-11-17 2009-05-27 インターナショナル・ビジネス・マシーンズ・コーポレーション 音声識別装置、発音矯正装置およびこれらの方法
FR2843973B1 (fr) 2002-08-30 2006-07-07 Agronomique Inst Nat Rech Cassettes d'expression procaryotes regulees par le zinc
US7198927B2 (en) 2004-12-22 2007-04-03 E. I. Du Pont De Nemours And Company Enzymatic production of glycolic acid
US7445917B2 (en) 2004-12-22 2008-11-04 E.I. Du Pont De Nemours And Company Process for producing glycolic acid from formaldehyde and hydrogen cyanide
CN102796770B (zh) * 2004-12-22 2014-06-04 纳幕尔杜邦公司 乙醇酸的酶促生产
US8178338B2 (en) 2005-07-01 2012-05-15 The Regents Of The University Of California Inducible expression vectors and methods of use thereof
JP4954985B2 (ja) 2006-05-09 2012-06-20 三井化学株式会社 補酵素合成強化によるグリコール酸の生産方法
EP2025760B1 (en) 2006-05-09 2013-06-05 Mitsui Chemicals, Inc. Method of producing glycolic acid by regenerating coenzyme
EP2027277B1 (en) 2006-06-09 2018-07-18 Metabolic Explorer Glycolic acid production by fermentation from renewable resources
WO2007140816A1 (en) * 2006-06-09 2007-12-13 Metabolic Explorer Glycolic acid production by fermentation from renewable resources
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EP2233562A1 (en) 2009-03-24 2010-09-29 Metabolic Explorer Method for producing high amount of glycolic acid by fermentation

Also Published As

Publication number Publication date
JP5882310B2 (ja) 2016-03-09
KR20130114079A (ko) 2013-10-16
BR112012031965A2 (pt) 2015-11-24
US20130089903A1 (en) 2013-04-11
CN103221548A (zh) 2013-07-24
WO2011157728A8 (en) 2013-01-17
CA2801823A1 (en) 2011-12-22
CA2801823C (en) 2019-07-30
EP2582828A1 (en) 2013-04-24
EP2582828B1 (en) 2015-07-15
US9121043B2 (en) 2015-09-01
WO2011157728A1 (en) 2011-12-22
CN103221548B (zh) 2017-04-12
KR101824282B1 (ko) 2018-01-31
JP2013528390A (ja) 2013-07-11

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