WO2012129548A3 - In situ expression of lipase for enzymatic production of alcohol esters during fermentation - Google Patents

In situ expression of lipase for enzymatic production of alcohol esters during fermentation Download PDF

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Publication number
WO2012129548A3
WO2012129548A3 PCT/US2012/030468 US2012030468W WO2012129548A3 WO 2012129548 A3 WO2012129548 A3 WO 2012129548A3 US 2012030468 W US2012030468 W US 2012030468W WO 2012129548 A3 WO2012129548 A3 WO 2012129548A3
Authority
WO
WIPO (PCT)
Prior art keywords
lipase
alcohol esters
during fermentation
enzymatic production
esters during
Prior art date
Application number
PCT/US2012/030468
Other languages
French (fr)
Other versions
WO2012129548A2 (en
Inventor
Robert Dicosimo
Arthur Leo Kruckeberg
Thomas Edwin VAN AKEN
Original Assignee
Butamax(Tm) Advanced Biofuels Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Butamax(Tm) Advanced Biofuels Llc filed Critical Butamax(Tm) Advanced Biofuels Llc
Priority to CN2012800146568A priority Critical patent/CN103459587A/en
Priority to EP12760739.8A priority patent/EP2689006A4/en
Priority to BR112013024329A priority patent/BR112013024329A2/en
Priority to JP2014501290A priority patent/JP2014509869A/en
Priority to AU2012230730A priority patent/AU2012230730A1/en
Priority to CA2829941A priority patent/CA2829941A1/en
Publication of WO2012129548A2 publication Critical patent/WO2012129548A2/en
Publication of WO2012129548A3 publication Critical patent/WO2012129548A3/en
Priority to ZA2013/06647A priority patent/ZA201306647B/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C12N9/20—Triglyceride splitting, e.g. by means of lipase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/649—Biodiesel, i.e. fatty acid alkyl esters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/16—Butanols
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Abstract

Disclosed herein are methods of producing alcohol esters during a fermentation by providing alcohol-producing microorganisms which further comprise an engineered polynucleotide encoding a polypeptide having lipase activity.
PCT/US2012/030468 2011-03-23 2012-03-23 In situ expression of lipase for enzymatic production of alcohol esters during fermentation WO2012129548A2 (en)

Priority Applications (7)

Application Number Priority Date Filing Date Title
CN2012800146568A CN103459587A (en) 2011-03-23 2012-03-23 In situ expression of lipase for enzymatic production of alcohol esters during fermentation
EP12760739.8A EP2689006A4 (en) 2011-03-23 2012-03-23 In situ expression of lipase for enzymatic production of alcohol esters during fermentation
BR112013024329A BR112013024329A2 (en) 2011-03-23 2012-03-23 methods, recombinant host cell, method of increasing tolerance of a microorganism producing alcohol to produced alcohol, butyl ester pressure during a fermentation, fermentation medium and animal feed product
JP2014501290A JP2014509869A (en) 2011-03-23 2012-03-23 In situ expression of lipase for enzymatic production of alcohol esters in fermentation
AU2012230730A AU2012230730A1 (en) 2011-03-23 2012-03-23 In situ expression of lipase for enzymatic production of alcohol esters during fermentation
CA2829941A CA2829941A1 (en) 2011-03-23 2012-03-23 In situ expression of lipase for enzymatic production of alcohol esters during fermentation
ZA2013/06647A ZA201306647B (en) 2011-03-23 2013-09-04 In situ expression of lipase for enzymatic production of alcohol esters during fermentation

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US201161466712P 2011-03-23 2011-03-23
US61/466,712 2011-03-23
US201161498292P 2011-06-17 2011-06-17
US61/498,292 2011-06-17

Publications (2)

Publication Number Publication Date
WO2012129548A2 WO2012129548A2 (en) 2012-09-27
WO2012129548A3 true WO2012129548A3 (en) 2013-03-14

Family

ID=46880081

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2012/030468 WO2012129548A2 (en) 2011-03-23 2012-03-23 In situ expression of lipase for enzymatic production of alcohol esters during fermentation

Country Status (8)

Country Link
EP (1) EP2689006A4 (en)
JP (1) JP2014509869A (en)
CN (1) CN103459587A (en)
AU (1) AU2012230730A1 (en)
BR (1) BR112013024329A2 (en)
CA (1) CA2829941A1 (en)
WO (1) WO2012129548A2 (en)
ZA (1) ZA201306647B (en)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP6093764B2 (en) * 2011-07-28 2017-03-08 ビュータマックス・アドバンスド・バイオフューエルズ・エルエルシー Ketoisovalerate decarboxylase enzyme and method of use thereof
US20160138050A1 (en) * 2013-07-16 2016-05-19 Butamax Advanced Biofuels Llc Yeast with increased butanol tolerance involving cell wall proteins
CN107488647B (en) * 2016-06-10 2019-12-10 广东溢多利生物科技股份有限公司 Lipase TTL mutant TTL-Gly60Glu with improved thermal stability and gene and application thereof
CN107488648B (en) * 2016-06-10 2019-12-10 广东溢多利生物科技股份有限公司 Lipase TTL mutant TTL-Arg59Ser/Gly60Glu/Ser61Asn with improved thermal stability, gene and application
CN107488646B (en) * 2016-06-10 2019-12-10 广东溢多利生物科技股份有限公司 Lipase mutant TTL-Arg59Ser/Gly60Glu/Ser61Asn/Ile62Val and gene and application thereof
CN107488644B (en) * 2016-06-10 2019-12-10 广东溢多利生物科技股份有限公司 Lipase TTL mutant TTL-Gly60Glu/Ser61Asn with improved thermal stability and gene and application thereof
CN107488645B (en) * 2016-06-10 2019-12-10 广东溢多利生物科技股份有限公司 Lipase TTL mutant TTL-Ser61Asn with improved thermal stability and gene and application thereof
CN106399286B (en) * 2016-10-18 2019-12-24 江南大学 Method for promoting pichia pastoris to produce alkaline pectinase
CN106701606B (en) * 2016-12-19 2021-03-26 广东利世康低碳科技有限公司 Genetic engineering candida utilis capable of degrading and utilizing kitchen waste and construction method thereof
WO2018218033A2 (en) 2017-05-24 2018-11-29 Mccurdy Alexander T Use of an esterase to enhance ethyl ester content in fermentation media
CN108277212B (en) * 2017-12-29 2019-12-10 广东溢多利生物科技股份有限公司 Lipase mutant Gly183Cys/Gly212Cys and gene and application thereof
CN108315312B (en) * 2018-04-11 2019-12-10 广东溢多利生物科技股份有限公司 Lipase TTL mutant with improved thermal stability and coding gene and application thereof
CA3182639A1 (en) 2018-06-11 2019-12-19 Poet Research, Inc. Methods of refining a grain oil composition feedstock, and related systems, compositions and uses
JPWO2021045234A1 (en) * 2019-09-06 2021-03-11
EP4192964A1 (en) * 2020-08-06 2023-06-14 POET Research, Inc. Endogenous lipase for metal reduction in distillers corn oil
WO2023225459A2 (en) 2022-05-14 2023-11-23 Novozymes A/S Compositions and methods for preventing, treating, supressing and/or eliminating phytopathogenic infestations and infections

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5780275A (en) * 1995-09-07 1998-07-14 Kansai Paint Co., Ltd. Coupled process of saccharide fermentation and microbial esterification
US20070134780A1 (en) * 2003-06-10 2007-06-14 Novozymes North America, Inc. Fermentation processes and compositions
US20080193991A1 (en) * 2007-02-13 2008-08-14 Water Solutions, Inc. Process for improving the yield and efficiency of an ethanol fermentation plant
WO2009149270A2 (en) * 2008-06-04 2009-12-10 E. I. Du Pont De Nemours And Company A method for producing butanol using two-phase extractive fermentation
US20100180491A1 (en) * 2007-06-29 2010-07-22 The Regents Of The University Of California Host Cells and Methods for Producing Isoprenyl Alkanoates
WO2011159998A2 (en) * 2010-06-18 2011-12-22 Butamax(Tm) Advanced Biofuels Llc Production of alcohol esters and in situ product removal during alcohol fermentation

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011063402A2 (en) * 2009-11-23 2011-05-26 Butamax(Tm) Advanced Biofuels Llc Method for producing butanol using extractive fermentation with osmolyte addition

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5780275A (en) * 1995-09-07 1998-07-14 Kansai Paint Co., Ltd. Coupled process of saccharide fermentation and microbial esterification
US20070134780A1 (en) * 2003-06-10 2007-06-14 Novozymes North America, Inc. Fermentation processes and compositions
US20080193991A1 (en) * 2007-02-13 2008-08-14 Water Solutions, Inc. Process for improving the yield and efficiency of an ethanol fermentation plant
US20100180491A1 (en) * 2007-06-29 2010-07-22 The Regents Of The University Of California Host Cells and Methods for Producing Isoprenyl Alkanoates
WO2009149270A2 (en) * 2008-06-04 2009-12-10 E. I. Du Pont De Nemours And Company A method for producing butanol using two-phase extractive fermentation
WO2011159998A2 (en) * 2010-06-18 2011-12-22 Butamax(Tm) Advanced Biofuels Llc Production of alcohol esters and in situ product removal during alcohol fermentation

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See also references of EP2689006A4 *

Also Published As

Publication number Publication date
EP2689006A4 (en) 2014-10-01
BR112013024329A2 (en) 2017-12-19
WO2012129548A2 (en) 2012-09-27
CA2829941A1 (en) 2012-09-27
EP2689006A2 (en) 2014-01-29
JP2014509869A (en) 2014-04-24
ZA201306647B (en) 2014-11-26
AU2012230730A1 (en) 2013-09-19
CN103459587A (en) 2013-12-18

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