BR112012018956A2 - processo para produção de uma proteína interferon alfa 5 - Google Patents

processo para produção de uma proteína interferon alfa 5

Info

Publication number
BR112012018956A2
BR112012018956A2 BR112012018956A BR112012018956A BR112012018956A2 BR 112012018956 A2 BR112012018956 A2 BR 112012018956A2 BR 112012018956 A BR112012018956 A BR 112012018956A BR 112012018956 A BR112012018956 A BR 112012018956A BR 112012018956 A2 BR112012018956 A2 BR 112012018956A2
Authority
BR
Brazil
Prior art keywords
protein
producing
interferon alfa
ifna5
infa5
Prior art date
Application number
BR112012018956A
Other languages
English (en)
Other versions
BR112012018956B1 (pt
Inventor
Vladas Algridas Bumelis
Original Assignee
Digna Biotech Sl
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Digna Biotech Sl filed Critical Digna Biotech Sl
Publication of BR112012018956A2 publication Critical patent/BR112012018956A2/pt
Publication of BR112012018956B1 publication Critical patent/BR112012018956B1/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/555—Interferons [IFN]
    • C07K14/56—IFN-alpha
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/38—Chemical stimulation of growth or activity by addition of chemical compounds which are not essential growth factors; Stimulation of growth by removal of a chemical compound

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Microbiology (AREA)
  • Toxicology (AREA)
  • General Engineering & Computer Science (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)

Abstract

processo para produção de uma proteína interferon alfa 5 é descrito um processo para produção de uma proteína interefron alfa 5 (infa5) através da expressão em uma ifna5 que produz célula hospedeira escherichia coli, em que incorporação de um resíduo de metionina extra na extremidade terminal-n da cadeia de polipeptídio é minimizada, como também a geração de suas espécies oxidades. a proteína ifna5 pode ser purificada através de um processo eficiente para render uma ifna5 biologicamente ativa.
BR112012018956-5A 2010-02-01 2011-01-31 Processo para produção de uma proteína interferon alfa 5 BR112012018956B1 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP10382019.7 2010-02-01
EP10382019 2010-02-01
PCT/ES2011/070057 WO2011092367A1 (es) 2010-02-01 2011-01-31 Procedimiento para la producción de interferón alfa 5

Publications (2)

Publication Number Publication Date
BR112012018956A2 true BR112012018956A2 (pt) 2015-09-15
BR112012018956B1 BR112012018956B1 (pt) 2022-05-31

Family

ID=43920729

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112012018956-5A BR112012018956B1 (pt) 2010-02-01 2011-01-31 Processo para produção de uma proteína interferon alfa 5

Country Status (12)

Country Link
US (1) US8709757B2 (pt)
EP (1) EP2532734B1 (pt)
JP (1) JP5830032B2 (pt)
KR (1) KR101858598B1 (pt)
CN (1) CN102869767B (pt)
AU (1) AU2011209506A1 (pt)
BR (1) BR112012018956B1 (pt)
CA (1) CA2788607C (pt)
MX (1) MX2012008893A (pt)
NZ (1) NZ601616A (pt)
RU (1) RU2575598C9 (pt)
WO (1) WO2011092367A1 (pt)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103864915B (zh) * 2012-12-07 2016-06-08 天津未名生物医药有限公司 纯化重组人干扰素α2b的方法和试剂盒
CN104356223B (zh) * 2014-11-26 2017-11-10 安徽安科生物工程（集团）股份有限公司 高纯度重组人干扰素α2b的制备方法
LT6861B (lt) 2021-04-02 2021-11-10 Kauno technologijos universitetas Maitinimo profilių, skirtų valdyti pusiau-periodinius rekombinantinių E. coli kultivavimo procesus, formavimo ir adaptavimo būdas

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4973479A (en) 1982-01-15 1990-11-27 Cetus Corporation Interferon-α61
IT1167610B (it) * 1982-01-19 1987-05-13 Cetus Corp Interfreron ibrido multiclasse, composizione farmaceutica che lo contiene e procedimento di produzione
US4511502A (en) 1982-12-22 1985-04-16 Genentech, Inc. Purification and activity assurance of precipitated heterologous proteins
US4855238A (en) * 1983-12-16 1989-08-08 Genentech, Inc. Recombinant gamma interferons having enhanced stability and methods therefor
DE3511011A1 (de) 1985-03-27 1986-10-02 Hoechst Ag, 6230 Frankfurt Verfahren zur isolierung und reinigung von (alpha)-interferonen
US4765903A (en) 1987-10-06 1988-08-23 Interferon Sciences, Inc. Purification of monomeric interferon
US5814485A (en) * 1995-06-06 1998-09-29 Chiron Corporation Production of interferon-β (IFN-β) in E. coli
AR005035A1 (es) * 1995-12-11 1999-04-07 Merck Patent Ges Mit Beschränkter Haftung Procedimiento para preparar proteinas recombinantes en e. coli, mediante fermentacion con gran concentracion de celulas.
ES2138565B1 (es) * 1998-05-13 2000-08-16 Inst Cientifico Tecnol Navarra Uso del interferon alfa 5 en el tratamiento de las hepatopatias viral es.
ITMI20012345A1 (it) 2001-11-08 2003-05-08 Internat Ct For Genetic En Gin Procedimento per la produzione di interferone alfa di grado terapeutico
ZA200504990B (en) * 2003-01-09 2006-08-30 Genentech Inc Purification of polypeptides
WO2005087940A1 (en) * 2004-03-11 2005-09-22 Wisconsin Alumni Research Foundation Genetically altered microorganisms with modified metabolism
ATE529442T1 (de) * 2005-06-03 2011-11-15 Ambrx Inc Verbesserte humane interferon-moleküle und ihre verwendungen
EP1990349A1 (en) 2007-05-09 2008-11-12 Institut Pasteur Method for the production of high-level soluble human recombinant interferon alpha in E. coli and vectors useful for such a production

Also Published As

Publication number Publication date
JP2013517788A (ja) 2013-05-20
MX2012008893A (es) 2013-02-27
NZ601616A (en) 2013-04-26
WO2011092367A1 (es) 2011-08-04
BR112012018956B1 (pt) 2022-05-31
US20120309057A1 (en) 2012-12-06
RU2012137428A (ru) 2014-03-10
KR20120125641A (ko) 2012-11-16
EP2532734A1 (en) 2012-12-12
CN102869767B (zh) 2016-03-09
EP2532734B1 (en) 2017-03-15
RU2575598C9 (ru) 2016-04-27
KR101858598B1 (ko) 2018-05-16
CA2788607A1 (en) 2011-08-04
RU2575598C2 (ru) 2016-02-20
CN102869767A (zh) 2013-01-09
CA2788607C (en) 2018-03-20
JP5830032B2 (ja) 2015-12-09
US8709757B2 (en) 2014-04-29
AU2011209506A1 (en) 2012-08-23

Similar Documents

Publication Publication Date Title
BRPI0800097A (pt) método de produção de xilitol através do emprego de hidrolisato contendo xilose e arabinose preparado a partir do subproduto da biomassa de frutas tropicais
AR086790A1 (es) Un microorganismo para la produccion de metionina con importacion de glucosa mejorada
PA8802301A1 (es) Compuestos que exhiben actividad antagonista del glucagon y agonistas del glp-1
PE20170950A1 (es) Polipeptidos del factor de crecimiento de fibroblastos 2 (fgf-21) modificados y usos de los mismos
BR112014016461A2 (pt) método para a estabilização de fibra de lignina para a posterior conversão em fibra de carbono
BR112017017525A2 (pt) produção de fdca catalisada por desidrogenase
BR112015004089A2 (pt) micro-organismos e métodos para intensificar a disponibilidade de equivalentes de redução na presença de metanol e para produzir 1,4-butanodiol relacionado com os mesmos
CO7170149A2 (es) Producción mejorada de derivados de ácidos grasos
CR11511A (es) Nuevos derivados de insulina con perfil tiempo/accion extremadamente retardado
BR112015012918A2 (pt) composições para cuidado oral contendo zinco e fluoreto estável
ES2575906T3 (es) Composiciones y métodos de producir metionina
CO6670566A2 (es) Derivados de tetrahidro- pirido-pirimidina
AR084377A1 (es) Metodos y composiciones relacionados con enzimas de alcohol graso biosintetico
BR112012013847A2 (pt) novos derivados de indolinona ciclopropano
CL2010001189A1 (es) Metodo para la preparacion del sulfato de calcio del residuo acido
GT201300252A (es) Alfa glucosidasa ácida modificada con procesamiento acelerado
EA201491155A1 (ru) Новые мутантные про-нрф и их применение для получения бета-нрф
BR112018007174A2 (pt) instrumento para higiene bucal com saliências condutoras
AR087093A1 (es) Composicion que mejora la palatabilidad que comprende aminoacidos libre para usar en alimentos para mascotas
BRPI0922066A8 (pt) “processos para a fabricação de metilmercaptopropionaldeído, bem como da metionina ou do hidroxianálogo da metionina”
BR112013010522A2 (pt) método para purificação de fator estimulador de colônias de granulócitos humano a partir recombinantes de e. coli
BR112016016539A2 (pt) micro-organismos recombinantes enriquecidos com carboidratos
BR112013027130A2 (pt) fitase, sequência de ácido nucleico isolada, célula hospedeira recombinante, organismo de produção recombinante, aditivo de alimentação animal, alimentação animal, e, uso de uma fitase
BR112015018259A2 (pt) Redução de resposta pró-inflamatória
MY164641A (en) Use of sulphur-containing heteroaromatic acid analogues as bactericides

Legal Events

Date Code Title Description
B06T Formal requirements before examination [chapter 6.20 patent gazette]
B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B06A Patent application procedure suspended [chapter 6.1 patent gazette]
B06G Technical and formal requirements: other requirements [chapter 6.7 patent gazette]
B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B06G Technical and formal requirements: other requirements [chapter 6.7 patent gazette]
B09A Decision: intention to grant [chapter 9.1 patent gazette]
B16A Patent or certificate of addition of invention granted [chapter 16.1 patent gazette]

Free format text: PRAZO DE VALIDADE: 20 (VINTE) ANOS CONTADOS A PARTIR DE 31/01/2011, OBSERVADAS AS CONDICOES LEGAIS. PATENTE CONCEDIDA CONFORME ADI 5.529/DF, QUE DETERMINA A ALTERACAO DO PRAZO DE CONCESSAO.