BR0101294A - Métodos de triagem de um composto que modula a atividade do canal de cálcio, e para bloqueadores e para estimuladores de icrac, kit para uso em um método, célula sanguìnea ou célula derivada do sangue, linfócito ou célula derivada de linfócito, ou mastócito ou célula derivada de mastócito, e, população de células imunes de roedores - Google Patents
Métodos de triagem de um composto que modula a atividade do canal de cálcio, e para bloqueadores e para estimuladores de icrac, kit para uso em um método, célula sanguìnea ou célula derivada do sangue, linfócito ou célula derivada de linfócito, ou mastócito ou célula derivada de mastócito, e, população de células imunes de roedoresInfo
- Publication number
- BR0101294A BR0101294A BR0101294-0A BR0101294A BR0101294A BR 0101294 A BR0101294 A BR 0101294A BR 0101294 A BR0101294 A BR 0101294A BR 0101294 A BR0101294 A BR 0101294A
- Authority
- BR
- Brazil
- Prior art keywords
- cell
- cells
- derived
- blood
- lymphocyte
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6897—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6872—Intracellular protein regulatory factors and their receptors, e.g. including ion channels
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2500/00—Screening for compounds of potential therapeutic value
- G01N2500/10—Screening for compounds of potential therapeutic value involving cells
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Organic Chemistry (AREA)
- Immunology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Wood Science & Technology (AREA)
- General Health & Medical Sciences (AREA)
- Urology & Nephrology (AREA)
- Microbiology (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Biochemistry (AREA)
- Hematology (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biophysics (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
"MéTODOS DE TRIAGEM DE UM COMPOSTO QUE MODULA A ATIVIDADE DO CANAL DE CáLCIO, E PARA BLOQUEADORES E PARA ESTIMULADORES DE ICRAC, KIT PARA USO EM UM MéTODO, CéLULA SANGuìNEA OU CéLULA DERIVADA DO SANGUE, LINFóCITO OU CéLULA DERIVADA DE LINFóCITO, OU MASTóCITO OU CéLULA DERIVADA DE MASTóCITO, POPULAçãO DE CéLULAS IMUNES DE ROEDORES". A presente invenção diz respeito a composições e métodos direcionados à triagem ou caracterização de compostos que modulam a atividade de canais de cálcio em células, preferivelmente canais ativados por liberação de cálcio nas células. As composições e métodos podem ser usados para produzir inibidores ou ativadores do dito canal, que representa condutores ou medicamentos terapêuticos candidatos para tratar várias condições patológicas. Mais especificamente, o método compreende (a) colocar em contato um composto de teste e um ativador de canal de cálcio, preferivelmente um ativador de Icrac com uma população de células que expressam canais de cálcio, de preferência células que expressam Icrac contendo uma construção repórter compreendendo um gene repórter sob o controle de um promotor indutível por NFAT, e (b) determinar a atividade do composto de teste no canal ativado por liberação de cálcio, mediante avaliação da expressão do gene repórter nas ditas células.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP00400923A EP1143013A1 (en) | 2000-04-03 | 2000-04-03 | Methods and compositions for screening Icrac modulators |
Publications (1)
Publication Number | Publication Date |
---|---|
BR0101294A true BR0101294A (pt) | 2001-11-06 |
Family
ID=8173631
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
BR0101294-0A BR0101294A (pt) | 2000-04-03 | 2001-04-02 | Métodos de triagem de um composto que modula a atividade do canal de cálcio, e para bloqueadores e para estimuladores de icrac, kit para uso em um método, célula sanguìnea ou célula derivada do sangue, linfócito ou célula derivada de linfócito, ou mastócito ou célula derivada de mastócito, e, população de células imunes de roedores |
Country Status (6)
Country | Link |
---|---|
US (2) | US6696267B2 (pt) |
EP (1) | EP1143013A1 (pt) |
JP (1) | JP2001314193A (pt) |
BR (1) | BR0101294A (pt) |
CA (1) | CA2342677A1 (pt) |
MX (1) | MXPA01003405A (pt) |
Families Citing this family (22)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP1511843B1 (en) * | 2002-06-07 | 2006-03-29 | Sophion Bioscience A/S | Screening methods |
US6929923B2 (en) * | 2002-07-23 | 2005-08-16 | Rigel Pharmaceuticals, Inc. | Modulators of leukocyte activation, BIC compositions and methods of use |
JP4383898B2 (ja) * | 2003-02-28 | 2009-12-16 | 株式会社リコー | 現像剤収容器、現像剤供給装置及び画像形成装置 |
US7645588B2 (en) | 2003-03-04 | 2010-01-12 | Calcimedica, Inc. | Composition comprising a cell comprising a STIM1 protein and an agent that modulates intracellular calcium and methods of use |
KR100776010B1 (ko) * | 2005-02-26 | 2007-11-16 | 한국과학기술연구원 | T-형 α1H 칼슘 채널의 고효율 억제제 검색 및 광범위 특성연구용 HEK293 세포주 |
JP2009533062A (ja) * | 2006-04-10 | 2009-09-17 | ザ・クイーンズ・メディカル・センター | Cracモジュレーターおよび創薬のためのその使用 |
US8507269B2 (en) * | 2007-05-24 | 2013-08-13 | Calcimedica, Inc. | Calcium channel proteins and uses thereof |
JP5782377B2 (ja) | 2008-08-27 | 2015-09-24 | カルシメディカ,インク. | 細胞内カルシウムを調節する化合物 |
US8377970B2 (en) | 2009-10-08 | 2013-02-19 | Rhizen Pharmaceuticals Sa | Modulators of calcium release-activated calcium channel |
US8993612B2 (en) | 2009-10-08 | 2015-03-31 | Rhizen Pharmaceuticals Sa | Modulators of calcium release-activated calcium channel and methods for treatment of non-small cell lung cancer |
JP2013511279A (ja) | 2009-11-20 | 2013-04-04 | アムジエン・インコーポレーテツド | 抗Orai1抗原結合蛋白及びその使用 |
US8754219B2 (en) | 2010-04-27 | 2014-06-17 | Calcimedica, Inc. | Compounds that modulate intracellular calcium |
JP6112486B2 (ja) | 2010-04-27 | 2017-04-12 | カルシメディカ,インク. | 細胞内カルシウムを調節する化合物 |
US10703722B2 (en) | 2010-04-27 | 2020-07-07 | Calcimedica, Inc. | Compounds that modulate intracellular calcium |
WO2015054283A1 (en) | 2013-10-08 | 2015-04-16 | Calcimedica, Inc. | Compounds that modulate intracellular calcium |
AR103807A1 (es) | 2015-02-27 | 2017-06-07 | Calcimedica Inc | Tratamiento de la pancreatitis |
US10478435B2 (en) | 2015-08-07 | 2019-11-19 | Calcimedica, Inc. | Use of CRAC channel inhibitors for the treatment of stroke and traumatic brain injury |
TW201902875A (zh) | 2017-03-15 | 2019-01-16 | 美商亞瑟尼克斯公司 | 聯芳基哌啶醯胺化合物及其使用方法 |
GB201713078D0 (en) * | 2017-08-15 | 2017-09-27 | Adaptimmune Ltd | T Cell Modification |
CN113557020A (zh) | 2018-09-14 | 2021-10-26 | 瑞真药业公司 | 包含crac抑制剂和皮质类固醇的组合物及其使用方法 |
CN112442510A (zh) * | 2020-11-29 | 2021-03-05 | 天津理工大学 | 一种耐糖高分泌型基因工程受体菌的构建方法 |
CN115369145B (zh) * | 2022-01-19 | 2024-03-01 | 中国人民解放军军事科学院军事医学研究院 | 一种筛查乙酰胆碱酯酶抑制剂的方法 |
Family Cites Families (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5670113A (en) * | 1991-12-20 | 1997-09-23 | Sibia Neurosciences, Inc. | Automated analysis equipment and assay method for detecting cell surface protein and/or cytoplasmic receptor function using same |
US5741657A (en) * | 1995-03-20 | 1998-04-21 | The Regents Of The University Of California | Fluorogenic substrates for β-lactamase and methods of use |
US6004808A (en) * | 1996-06-21 | 1999-12-21 | Aurora Biosciences Corporation | Promiscuous G-protein compositions and their use |
AU4170597A (en) * | 1996-08-30 | 1998-03-19 | Regents Of The University Of California, The | Method and compounds for controlling capacitative calcium ion entry into mammalian cells |
AU776672B2 (en) * | 1998-12-30 | 2004-09-16 | Beth Israel Deaconess Medical Center | Characterization of a calcium channel family |
-
2000
- 2000-04-03 EP EP00400923A patent/EP1143013A1/en not_active Withdrawn
-
2001
- 2001-03-30 US US09/821,811 patent/US6696267B2/en not_active Expired - Fee Related
- 2001-04-02 BR BR0101294-0A patent/BR0101294A/pt not_active IP Right Cessation
- 2001-04-02 MX MXPA01003405A patent/MXPA01003405A/es unknown
- 2001-04-02 JP JP2001103164A patent/JP2001314193A/ja active Pending
- 2001-04-02 CA CA002342677A patent/CA2342677A1/en not_active Abandoned
-
2003
- 2003-10-24 US US10/691,553 patent/US20040121381A1/en not_active Abandoned
Also Published As
Publication number | Publication date |
---|---|
MXPA01003405A (es) | 2005-06-30 |
CA2342677A1 (en) | 2001-10-03 |
EP1143013A1 (en) | 2001-10-10 |
US20040121381A1 (en) | 2004-06-24 |
US20020034728A1 (en) | 2002-03-21 |
US6696267B2 (en) | 2004-02-24 |
JP2001314193A (ja) | 2001-11-13 |
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Free format text: REFERENTE A 6O, 7O E 8O ANUIDADES. |
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B08K | Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette] |
Free format text: REFERENTE AO DESPACHO 8.6 DA RPI 2012 DE 28/07/2009. |