BG102710A - Метод за контролиране на хемотерапия на нiv-положителни пациенти, основан на фенотипна чувствителност към ле- карства на човешки нiv-щамове - Google Patents

Метод за контролиране на хемотерапия на нiv-положителни пациенти, основан на фенотипна чувствителност към ле- карства на човешки нiv-щамове

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Publication number
BG102710A
BG102710A BG102710A BG10271098A BG102710A BG 102710 A BG102710 A BG 102710A BG 102710 A BG102710 A BG 102710A BG 10271098 A BG10271098 A BG 10271098A BG 102710 A BG102710 A BG 102710A
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Bulgaria
Prior art keywords
hiv
chimerical
inhibitor
data
type
Prior art date
Application number
BG102710A
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English (en)
Inventor
Bethune Marie-Pierre De
Kurt Hertogs
Rudi Pauwels
Original Assignee
Virco N.V.
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Publication date
Application filed by Virco N.V. filed Critical Virco N.V.
Publication of BG102710A publication Critical patent/BG102710A/bg

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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
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    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
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    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5014—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing toxicity
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/502—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
    • G01N33/5047—Cells of the immune system
    • G01N33/505—Cells of the immune system involving T-cells
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    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/16011—Human Immunodeficiency Virus, HIV
    • C—CHEMISTRY; METALLURGY
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    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/16011—Human Immunodeficiency Virus, HIV
    • C12N2740/16211—Human Immunodeficiency Virus, HIV concerning HIV gagpol
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A90/00—Technologies having an indirect contribution to adaptation to climate change
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  • Health & Medical Sciences (AREA)
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  • General Physics & Mathematics (AREA)
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  • Food Science & Technology (AREA)
  • Toxicology (AREA)
  • Tropical Medicine & Parasitology (AREA)
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  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Peptides Or Proteins (AREA)

Abstract

Методът осигурява фенотипна информация за индивидуални, заразени с НIV пациенти в широки мащаби икономично и бързо. Той е приложим към всички наличнив момента химиотерапевтични режими и може да бъдееднакво приложим и при бъдещи такива режими. Методът включва трансфекция на клетъчна линия, податлива на инфекция с НIV, с последователност, за предпочитане тази, която кодира RT и протеаза от pol гена на НIV, получен от пациент, и с HIV-ДНК конструкт, от който последователността е делетирана. Трансфектираните клетки се култивират, така че да се създаде резерв от химерични вируси, след което се оценява тяхната фенотипна чувствителност към инхибитор на ензима, кодиран от pol гена на НIV, като в допълнение се определя стойност. След това се конструира набор от данни, състоящ се от стойността зачувствителност на химеричния вирус и съответната стойност за химеричния див тип щам НIV, повтаря се оценката на чувствителността за най-малко още два инхибитора и по такъв начин се конструират като цяло най-малко три такива набора от данни. Наборите от данни се представят в двудименсиална или тридименсиална графична форма, така че разликите между чувствителностите на химеричните и дивите типове за всеки един набор от данни да осигуряват визуално измерване на устойчивостта на химеричния резерв на третиране с въпросния инхибитор. След това се подбира оптималният инхибитор(и) въз основа на графичното представяне на измерената устойчивост.
BG102710A 1996-01-26 1998-08-20 Метод за контролиране на хемотерапия на нiv-положителни пациенти, основан на фенотипна чувствителност към ле- карства на човешки нiv-щамове BG102710A (bg)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP96200175 1996-01-26
PCT/IB1997/000071 WO1997027480A1 (en) 1996-01-26 1997-01-24 Method of managing the chemotherapy of patients who are hiv positive based on the phenotypic drug sensitivity of human hiv strains

Publications (1)

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BG102710A true BG102710A (bg) 1999-03-31

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US (3) US6221578B1 (bg)
EP (1) EP0877937B1 (bg)
KR (1) KR100495690B1 (bg)
CN (2) CN1209875A (bg)
AT (1) ATE217971T1 (bg)
AU (1) AU717755B2 (bg)
BG (1) BG102710A (bg)
BR (1) BR9707204A (bg)
CZ (1) CZ292899B6 (bg)
DE (1) DE69712731T2 (bg)
ES (1) ES2177922T3 (bg)
HU (1) HU226203B1 (bg)
IL (1) IL125442A (bg)
IS (1) IS4799A (bg)
NO (1) NO321329B1 (bg)
NZ (1) NZ325912A (bg)
PL (1) PL186473B1 (bg)
SK (1) SK283878B6 (bg)
TR (1) TR199801443T2 (bg)
WO (1) WO1997027480A1 (bg)
ZA (1) ZA97669B (bg)

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NO321329B1 (no) 2006-04-24
IL125442A (en) 2002-03-10
PL328069A1 (en) 1999-01-04
HUP9902618A3 (en) 2001-04-28
KR100495690B1 (ko) 2005-11-08
CZ233598A3 (cs) 1999-06-16
ATE217971T1 (de) 2002-06-15
CZ292899B6 (cs) 2003-12-17
NO983300L (no) 1998-09-25
US6221578B1 (en) 2001-04-24
AU717755B2 (en) 2000-03-30
ZA97669B (en) 1998-06-25
AU1316897A (en) 1997-08-20
KR19990082027A (ko) 1999-11-15
HUP9902618A2 (hu) 1999-12-28
NO983300D0 (no) 1998-07-16
NZ325912A (en) 1999-01-28
PL186473B1 (pl) 2004-01-30
EP0877937B1 (en) 2002-05-22
US6528251B2 (en) 2003-03-04
SK100298A3 (en) 1999-02-11
US20020042679A1 (en) 2002-04-11
DE69712731D1 (de) 2002-06-27
IL125442A0 (en) 1999-03-12
DE69712731T2 (de) 2003-02-06
CN1991365A (zh) 2007-07-04
IS4799A (is) 1998-07-20
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BR9707204A (pt) 1999-12-28
CN1209875A (zh) 1999-03-03
HU226203B1 (en) 2008-06-30
TR199801443T2 (xx) 1998-10-21
ES2177922T3 (es) 2002-12-16
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US20030152917A1 (en) 2003-08-14
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