BE899661A - Procede d'augmentation de la production d'antibiotique par recombinaison genetique in vivo. - Google Patents

Procede d'augmentation de la production d'antibiotique par recombinaison genetique in vivo.

Info

Publication number
BE899661A
BE899661A BE1/11023A BE1011023A BE899661A BE 899661 A BE899661 A BE 899661A BE 1/11023 A BE1/11023 A BE 1/11023A BE 1011023 A BE1011023 A BE 1011023A BE 899661 A BE899661 A BE 899661A
Authority
BE
Belgium
Prior art keywords
vivo
antibiotic
production
strains
antibiotics
Prior art date
Application number
BE1/11023A
Other languages
English (en)
Original Assignee
Biogal Gyogyszergyar
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Biogal Gyogyszergyar filed Critical Biogal Gyogyszergyar
Publication of BE899661A publication Critical patent/BE899661A/fr

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/02—Preparation of hybrid cells by fusion of two or more cells, e.g. protoplast fusion
    • C12N15/03—Bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/44—Preparation of O-glycosides, e.g. glucosides
    • C12P19/46—Preparation of O-glycosides, e.g. glucosides having an oxygen atom of the saccharide radical bound to a cyclohexyl radical, e.g. kasugamycin
    • C12P19/48—Preparation of O-glycosides, e.g. glucosides having an oxygen atom of the saccharide radical bound to a cyclohexyl radical, e.g. kasugamycin the cyclohexyl radical being substituted by two or more nitrogen atoms, e.g. destomycin, neamin
    • C12P19/485—Having two saccharide radicals bound through only oxygen to non-adjacent ring carbons of the cyclohexyl radical, e.g. gentamycin, kanamycin, sisomycin, verdamycin, mutamycin, tobramycin, nebramycin, antibiotics 66-40B, 66-40D, XK-62-2, 66-40, G-418, G-52
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/465—Streptomyces
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/822—Microorganisms using bacteria or actinomycetales
    • Y10S435/886—Streptomyces

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Procédé de préparation de bouillons de fermentation contenant des antibiotiques du type aminoglucoside par la recombinaison génétique in vivo de souches productrices d'antibiotique à matériel génétique modifié, procédé caractérisé par le fait qu'après une préculture dans un milieu nutritif contenant du saccharose et des ions calcium et magnésium, on forme des protoplastes que l'on fusionne alors et que l'on régénère. On isole les souches à matériel gnétrique modifié, on vérifie leur capacité de production d'antibiotique, on sélectionne les souches à productivité modifiée et on les cultive. Le procédé selon l'invention convient pour modifier qualitativement et quantitativement la productivité d'antibiotique d'une souche.
BE1/11023A 1983-05-16 1984-05-15 Procede d'augmentation de la production d'antibiotique par recombinaison genetique in vivo. BE899661A (fr)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
HU831678A HU194306B (en) 1983-05-16 1983-05-16 Process for influencing biosynthesis of antibiotics with in vivo genetical recombination

Publications (1)

Publication Number Publication Date
BE899661A true BE899661A (fr) 1984-11-16

Family

ID=10955644

Family Applications (1)

Application Number Title Priority Date Filing Date
BE1/11023A BE899661A (fr) 1983-05-16 1984-05-15 Procede d'augmentation de la production d'antibiotique par recombinaison genetique in vivo.

Country Status (8)

Country Link
US (1) US4729951A (fr)
BE (1) BE899661A (fr)
CH (1) CH662582A5 (fr)
DE (1) DE3418187A1 (fr)
FI (1) FI79551C (fr)
FR (1) FR2546180B1 (fr)
GB (1) GB2146015B (fr)
HU (1) HU194306B (fr)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5149639A (en) * 1986-03-24 1992-09-22 Abbott Laboratories Biologically pure cultures of streptomyces and use thereof in macrolide antibiotic production
US5670350A (en) * 1990-08-20 1997-09-23 Novartis Finance Corporation Genomic DNA encoding a pseudomonas global transcriptional activation element and its use in activating gene expression
US5639949A (en) * 1990-08-20 1997-06-17 Ciba-Geigy Corporation Genes for the synthesis of antipathogenic substances
US5662898A (en) * 1990-08-20 1997-09-02 Ciba-Geigy Corporation Genes for the synthesis of antipathogenic substances
US6117670A (en) * 1994-06-08 2000-09-12 Novartis Finance Corporation Pyrrolnitrin biosynthesis genes and uses thereof
US5888744A (en) * 1995-08-24 1999-03-30 The United States Of America, As Represented By The Secretary Of Agriculture Detection and separation of aminoglycosides by binding to immobilized lysozyme or α-lactalbumin
DK1717322T3 (da) * 1997-01-17 2012-10-22 Codexis Mayflower Holdings Llc Udvikling af hele celler og organismer ved rekursiv sekvensrekombination
US7148054B2 (en) * 1997-01-17 2006-12-12 Maxygen, Inc. Evolution of whole cells and organisms by recursive sequence recombination
US6326204B1 (en) 1997-01-17 2001-12-04 Maxygen, Inc. Evolution of whole cells and organisms by recursive sequence recombination
CN113403299A (zh) * 2021-07-21 2021-09-17 大连工业大学 一种选育高产抗菌脂肽的枯草芽孢杆菌的方法

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4159226A (en) * 1977-07-01 1979-06-26 Eli Lilly And Company Method of obtaining streptomyces protoplasts capable of efficient cell regeneration
GB1602074A (en) * 1977-07-01 1981-11-04 Lilly Co Eli Method of facilitating genetic exchange in streptomyces and nocardia by protoplast fusion
HU184125B (en) * 1978-11-13 1984-07-30 Gyogyszerkutato Intezet New genetic process for preparing antibiotic producing micromono spora strains

Also Published As

Publication number Publication date
FI79551C (fi) 1990-01-10
US4729951A (en) 1988-03-08
FI841951A0 (fi) 1984-05-15
FR2546180A1 (fr) 1984-11-23
DE3418187A1 (de) 1985-03-28
FR2546180B1 (fr) 1987-05-29
CH662582A5 (de) 1987-10-15
FI841951A (fi) 1984-11-17
GB8412364D0 (en) 1984-06-20
HU194306B (en) 1988-01-28
FI79551B (fi) 1989-09-29
DE3418187C2 (fr) 1991-12-19
HUT34235A (en) 1985-02-28
GB2146015B (en) 1987-03-25
GB2146015A (en) 1985-04-11

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Legal Events

Date Code Title Description
RE Patent lapsed

Owner name: BIOGAL GYOGYSZERGYAR

Effective date: 19960531