AU8871891A - Direct cloning of pcr amplified nucleic acids - Google Patents

Direct cloning of pcr amplified nucleic acids

Info

Publication number
AU8871891A
AU8871891A AU88718/91A AU8871891A AU8871891A AU 8871891 A AU8871891 A AU 8871891A AU 88718/91 A AU88718/91 A AU 88718/91A AU 8871891 A AU8871891 A AU 8871891A AU 8871891 A AU8871891 A AU 8871891A
Authority
AU
Australia
Prior art keywords
nucleic acids
pcr amplified
amplified nucleic
direct cloning
cloning
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU88718/91A
Other languages
English (en)
Inventor
Joseph M. Fernandez
Corinna Hernstadt
David A Mead
Lloyd Smith
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
MOLECULAR CHIMERICS Corp
Life Technologies Corp
Original Assignee
MOLECULAR CHIMERICS CORP
Invitrogen Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by MOLECULAR CHIMERICS CORP, Invitrogen Corp filed Critical MOLECULAR CHIMERICS CORP
Publication of AU8871891A publication Critical patent/AU8871891A/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
AU88718/91A 1990-09-27 1991-09-27 Direct cloning of pcr amplified nucleic acids Abandoned AU8871891A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US589817 1984-03-15
US58981790A 1990-09-27 1990-09-27

Publications (1)

Publication Number Publication Date
AU8871891A true AU8871891A (en) 1992-04-28

Family

ID=24359662

Family Applications (1)

Application Number Title Priority Date Filing Date
AU88718/91A Abandoned AU8871891A (en) 1990-09-27 1991-09-27 Direct cloning of pcr amplified nucleic acids

Country Status (6)

Country Link
US (2) US5487993A (fr)
EP (4) EP1396540B1 (fr)
AU (1) AU8871891A (fr)
DE (3) DE69133559T2 (fr)
HK (1) HK1064124A1 (fr)
WO (1) WO1992006189A1 (fr)

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US5856144A (en) * 1997-06-18 1999-01-05 Novagen, Inc. Direct cloning of DNA fragments

Also Published As

Publication number Publication date
EP1396540A3 (fr) 2004-06-09
EP0550693A4 (fr) 1994-04-27
EP0738779A2 (fr) 1996-10-23
WO1992006189A1 (fr) 1992-04-16
EP1396540B1 (fr) 2007-02-07
HK1064124A1 (en) 2005-01-21
EP0738779B1 (fr) 2004-05-19
US5827657A (en) 1998-10-27
EP0738779A3 (fr) 1997-05-07
DE69133389T2 (de) 2005-06-02
DE69133559D1 (de) 2007-03-22
DE69133389D1 (de) 2004-06-24
EP1790723A3 (fr) 2008-10-22
DE69119083T2 (de) 1996-10-17
US5487993A (en) 1996-01-30
DE69133559T2 (de) 2007-11-22
EP0550693A1 (fr) 1993-07-14
EP1396540A2 (fr) 2004-03-10
DE69119083D1 (de) 1996-05-30
EP1790723A2 (fr) 2007-05-30
EP0550693B1 (fr) 1996-04-24

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