AU752174B2 - Fast measuring device of enzymatic activity - Google Patents
Fast measuring device of enzymatic activity Download PDFInfo
- Publication number
- AU752174B2 AU752174B2 AU51727/99A AU5172799A AU752174B2 AU 752174 B2 AU752174 B2 AU 752174B2 AU 51727/99 A AU51727/99 A AU 51727/99A AU 5172799 A AU5172799 A AU 5172799A AU 752174 B2 AU752174 B2 AU 752174B2
- Authority
- AU
- Australia
- Prior art keywords
- enzyme
- enzymatic activity
- activity
- solid
- measure
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 230000002255 enzymatic effect Effects 0.000 title claims abstract description 15
- 108090000790 Enzymes Proteins 0.000 claims abstract description 25
- 102000004190 Enzymes Human genes 0.000 claims abstract description 25
- 239000000872 buffer Substances 0.000 claims abstract description 15
- 230000000694 effects Effects 0.000 claims abstract description 14
- 239000007787 solid Substances 0.000 claims abstract description 14
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 12
- 239000000758 substrate Substances 0.000 claims abstract description 9
- 239000007788 liquid Substances 0.000 claims abstract description 6
- 239000007791 liquid phase Substances 0.000 claims abstract description 6
- 238000000034 method Methods 0.000 claims abstract description 6
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims description 9
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 9
- BDAGIHXWWSANSR-UHFFFAOYSA-N methanoic acid Natural products OC=O BDAGIHXWWSANSR-UHFFFAOYSA-N 0.000 claims description 6
- 239000001124 (E)-prop-1-ene-1,2,3-tricarboxylic acid Substances 0.000 claims description 3
- IVLXQGJVBGMLRR-UHFFFAOYSA-N 2-aminoacetic acid;hydron;chloride Chemical compound Cl.NCC(O)=O IVLXQGJVBGMLRR-UHFFFAOYSA-N 0.000 claims description 3
- OSWFIVFLDKOXQC-UHFFFAOYSA-N 4-(3-methoxyphenyl)aniline Chemical compound COC1=CC=CC(C=2C=CC(N)=CC=2)=C1 OSWFIVFLDKOXQC-UHFFFAOYSA-N 0.000 claims description 3
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 claims description 3
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 claims description 3
- 239000004280 Sodium formate Substances 0.000 claims description 3
- 229940091181 aconitic acid Drugs 0.000 claims description 3
- GTZCVFVGUGFEME-IWQZZHSRSA-N cis-aconitic acid Chemical compound OC(=O)C\C(C(O)=O)=C\C(O)=O GTZCVFVGUGFEME-IWQZZHSRSA-N 0.000 claims description 3
- 235000019253 formic acid Nutrition 0.000 claims description 3
- 229960001269 glycine hydrochloride Drugs 0.000 claims description 3
- 239000001632 sodium acetate Substances 0.000 claims description 3
- 235000017281 sodium acetate Nutrition 0.000 claims description 3
- 235000019254 sodium formate Nutrition 0.000 claims description 3
- HLBBKKJFGFRGMU-UHFFFAOYSA-M sodium formate Chemical compound [Na+].[O-]C=O HLBBKKJFGFRGMU-UHFFFAOYSA-M 0.000 claims description 3
- GTZCVFVGUGFEME-UHFFFAOYSA-N trans-aconitic acid Natural products OC(=O)CC(C(O)=O)=CC(O)=O GTZCVFVGUGFEME-UHFFFAOYSA-N 0.000 claims description 3
- 239000011324 bead Substances 0.000 claims description 2
- 239000007790 solid phase Substances 0.000 claims description 2
- 235000019728 animal nutrition Nutrition 0.000 claims 1
- 229940088598 enzyme Drugs 0.000 description 17
- 101710121765 Endo-1,4-beta-xylanase Proteins 0.000 description 13
- 238000005259 measurement Methods 0.000 description 10
- 239000008187 granular material Substances 0.000 description 7
- 238000006243 chemical reaction Methods 0.000 description 6
- 241001465754 Metazoa Species 0.000 description 3
- DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 description 2
- 229920002678 cellulose Polymers 0.000 description 2
- 239000001913 cellulose Substances 0.000 description 2
- 238000006047 enzymatic hydrolysis reaction Methods 0.000 description 2
- 239000007937 lozenge Substances 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 239000000843 powder Substances 0.000 description 2
- KUIXZSYWBHSYCN-UHFFFAOYSA-L remazol brilliant blue r Chemical compound [Na+].[Na+].C1=C(S([O-])(=O)=O)C(N)=C2C(=O)C3=CC=CC=C3C(=O)C2=C1NC1=CC=CC(S(=O)(=O)CCOS([O-])(=O)=O)=C1 KUIXZSYWBHSYCN-UHFFFAOYSA-L 0.000 description 2
- 235000019333 sodium laurylsulphate Nutrition 0.000 description 2
- 238000005507 spraying Methods 0.000 description 2
- 239000004094 surface-active agent Substances 0.000 description 2
- 229920001221 xylan Polymers 0.000 description 2
- 150000004823 xylans Chemical class 0.000 description 2
- FYGDTMLNYKFZSV-URKRLVJHSA-N (2s,3r,4s,5s,6r)-2-[(2r,4r,5r,6s)-4,5-dihydroxy-2-(hydroxymethyl)-6-[(2r,4r,5r,6s)-4,5,6-trihydroxy-2-(hydroxymethyl)oxan-3-yl]oxyoxan-3-yl]oxy-6-(hydroxymethyl)oxane-3,4,5-triol Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1OC1[C@@H](CO)O[C@@H](OC2[C@H](O[C@H](O)[C@H](O)[C@H]2O)CO)[C@H](O)[C@H]1O FYGDTMLNYKFZSV-URKRLVJHSA-N 0.000 description 1
- 229920002498 Beta-glucan Polymers 0.000 description 1
- 101710130006 Beta-glucanase Proteins 0.000 description 1
- 108010059892 Cellulase Proteins 0.000 description 1
- 229920001503 Glucan Polymers 0.000 description 1
- 239000004141 Sodium laurylsulphate Substances 0.000 description 1
- 241001136494 Talaromyces funiculosus Species 0.000 description 1
- 241000499912 Trichoderma reesei Species 0.000 description 1
- 241000209140 Triticum Species 0.000 description 1
- 235000021307 Triticum Nutrition 0.000 description 1
- 235000004240 Triticum spelta Nutrition 0.000 description 1
- 229920002000 Xyloglucan Polymers 0.000 description 1
- 239000008351 acetate buffer Substances 0.000 description 1
- 238000011088 calibration curve Methods 0.000 description 1
- 229940106157 cellulase Drugs 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 230000001934 delay Effects 0.000 description 1
- 235000019621 digestibility Nutrition 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- 230000007071 enzymatic hydrolysis Effects 0.000 description 1
- 230000007717 exclusion Effects 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 238000005469 granulation Methods 0.000 description 1
- 230000003179 granulation Effects 0.000 description 1
- 235000019239 indanthrene blue RS Nutrition 0.000 description 1
- UHOKSCJSTAHBSO-UHFFFAOYSA-N indanthrone blue Chemical compound C1=CC=C2C(=O)C3=CC=C4NC5=C6C(=O)C7=CC=CC=C7C(=O)C6=CC=C5NC4=C3C(=O)C2=C1 UHOKSCJSTAHBSO-UHFFFAOYSA-N 0.000 description 1
- 238000000691 measurement method Methods 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 230000035484 reaction time Effects 0.000 description 1
- 238000002798 spectrophotometry method Methods 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 229920002994 synthetic fiber Polymers 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| FR98/10533 | 1998-08-19 | ||
| FR9810533A FR2782523B1 (fr) | 1998-08-19 | 1998-08-19 | Dispositif de mesure rapide de l'activite enzymatique |
| PCT/FR1999/001990 WO2000011136A1 (fr) | 1998-08-19 | 1999-08-16 | Dispositif de mesure rapide de l'activite enzymatique |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| AU5172799A AU5172799A (en) | 2000-03-14 |
| AU752174B2 true AU752174B2 (en) | 2002-09-05 |
Family
ID=9529765
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AU51727/99A Expired AU752174B2 (en) | 1998-08-19 | 1999-08-16 | Fast measuring device of enzymatic activity |
Country Status (19)
| Country | Link |
|---|---|
| US (1) | US6821722B1 (ru) |
| EP (1) | EP1105456B1 (ru) |
| JP (1) | JP4531258B2 (ru) |
| KR (1) | KR20010072751A (ru) |
| CN (1) | CN1181182C (ru) |
| AT (1) | ATE254169T1 (ru) |
| AU (1) | AU752174B2 (ru) |
| BR (1) | BR9914294B1 (ru) |
| CA (1) | CA2341581C (ru) |
| DE (1) | DE69912806T2 (ru) |
| DK (1) | DK1105456T3 (ru) |
| EA (1) | EA002734B1 (ru) |
| ES (1) | ES2209474T3 (ru) |
| FR (1) | FR2782523B1 (ru) |
| ID (1) | ID28921A (ru) |
| MX (1) | MXPA01001628A (ru) |
| PT (1) | PT1105456E (ru) |
| SA (1) | SA00200919B1 (ru) |
| WO (1) | WO2000011136A1 (ru) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1646725A2 (en) * | 2003-07-07 | 2006-04-19 | Syngenta Participations AG | Reagents, methods and kits for detecting feed enzymes |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4277561A (en) * | 1976-02-19 | 1981-07-07 | Laboratoire De Recherche Api | Support for the determination of enzyme activities and process |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| IT8505253A0 (it) * | 1985-11-15 | 1985-11-15 | Copan Srl | Tampone con serbatoio di terreno di trasporto per il prelievo e il trasferimento in provetta di campioni di sostenze da analizzare. |
| ATE97450T1 (de) * | 1989-02-02 | 1993-12-15 | Abbott Lab | Verfahren und vorrichtung zur quantitativen chromatographierung. |
| JPH04229168A (ja) * | 1990-12-26 | 1992-08-18 | Hoechst Japan Ltd | 酵素の活性と酵素反応阻害物質の活性を測定するための器具及び方法 |
| GB2301103B (en) * | 1995-05-23 | 1999-12-22 | Danisco | An enzyme system comprising ferulic acid esterase |
| US6001587A (en) * | 1997-04-08 | 1999-12-14 | The United States Of America As Represented By The Secretary Of The Navy | Chemically specific patterning on solid surfaces using surface immobilized enzymes |
-
1998
- 1998-08-19 FR FR9810533A patent/FR2782523B1/fr not_active Expired - Fee Related
-
1999
- 1999-08-16 DE DE69912806T patent/DE69912806T2/de not_active Expired - Lifetime
- 1999-08-16 DK DK99936736T patent/DK1105456T3/da active
- 1999-08-16 AU AU51727/99A patent/AU752174B2/en not_active Expired
- 1999-08-16 MX MXPA01001628A patent/MXPA01001628A/es active IP Right Grant
- 1999-08-16 PT PT99936736T patent/PT1105456E/pt unknown
- 1999-08-16 ES ES99936736T patent/ES2209474T3/es not_active Expired - Lifetime
- 1999-08-16 WO PCT/FR1999/001990 patent/WO2000011136A1/fr not_active Application Discontinuation
- 1999-08-16 CA CA2341581A patent/CA2341581C/fr not_active Expired - Lifetime
- 1999-08-16 KR KR1020017002077A patent/KR20010072751A/ko not_active Application Discontinuation
- 1999-08-16 BR BRPI9914294-5A patent/BR9914294B1/pt not_active IP Right Cessation
- 1999-08-16 AT AT99936736T patent/ATE254169T1/de active
- 1999-08-16 EA EA200100247A patent/EA002734B1/ru not_active IP Right Cessation
- 1999-08-16 EP EP99936736A patent/EP1105456B1/fr not_active Expired - Lifetime
- 1999-08-16 JP JP2000566393A patent/JP4531258B2/ja not_active Expired - Fee Related
- 1999-08-16 CN CNB998097853A patent/CN1181182C/zh not_active Expired - Lifetime
- 1999-08-16 US US09/763,018 patent/US6821722B1/en not_active Expired - Lifetime
- 1999-10-16 ID IDW20010613A patent/ID28921A/id unknown
-
2000
- 2000-02-01 SA SA00200919A patent/SA00200919B1/ar unknown
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4277561A (en) * | 1976-02-19 | 1981-07-07 | Laboratoire De Recherche Api | Support for the determination of enzyme activities and process |
Also Published As
| Publication number | Publication date |
|---|---|
| JP2002523038A (ja) | 2002-07-30 |
| EP1105456B1 (fr) | 2003-11-12 |
| EA200100247A1 (ru) | 2001-08-27 |
| EP1105456A1 (fr) | 2001-06-13 |
| ATE254169T1 (de) | 2003-11-15 |
| ES2209474T3 (es) | 2004-06-16 |
| FR2782523A1 (fr) | 2000-02-25 |
| DE69912806D1 (de) | 2003-12-18 |
| CA2341581A1 (fr) | 2000-03-02 |
| MXPA01001628A (es) | 2002-04-08 |
| AU5172799A (en) | 2000-03-14 |
| SA00200919B1 (ar) | 2006-06-10 |
| US6821722B1 (en) | 2004-11-23 |
| WO2000011136A1 (fr) | 2000-03-02 |
| FR2782523B1 (fr) | 2000-10-06 |
| JP4531258B2 (ja) | 2010-08-25 |
| BR9914294A (pt) | 2001-11-06 |
| ID28921A (id) | 2001-07-12 |
| CA2341581C (fr) | 2010-10-12 |
| DK1105456T3 (da) | 2004-02-23 |
| BR9914294B1 (pt) | 2011-01-25 |
| CN1181182C (zh) | 2004-12-22 |
| PT1105456E (pt) | 2004-04-30 |
| CN1312854A (zh) | 2001-09-12 |
| KR20010072751A (ko) | 2001-07-31 |
| DE69912806T2 (de) | 2004-09-23 |
| EA002734B1 (ru) | 2002-08-29 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FGA | Letters patent sealed or granted (standard patent) | ||
| MK14 | Patent ceased section 143(a) (annual fees not paid) or expired |