AU2020102730A4 - Large-scale artificial breeding method for neoseiulu californicus - Google Patents
Large-scale artificial breeding method for neoseiulu californicus Download PDFInfo
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- AU2020102730A4 AU2020102730A4 AU2020102730A AU2020102730A AU2020102730A4 AU 2020102730 A4 AU2020102730 A4 AU 2020102730A4 AU 2020102730 A AU2020102730 A AU 2020102730A AU 2020102730 A AU2020102730 A AU 2020102730A AU 2020102730 A4 AU2020102730 A4 AU 2020102730A4
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- Australia
- Prior art keywords
- neoseiulu
- californicus
- breeding
- prey
- room
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- 238000009395 breeding Methods 0.000 title claims abstract description 54
- 241001658758 Neoseiulus Species 0.000 title claims abstract description 51
- 230000001488 breeding effect Effects 0.000 claims abstract description 41
- 241000916142 Tetranychus turkestani Species 0.000 claims abstract description 15
- 239000011521 glass Substances 0.000 claims abstract description 10
- 239000001963 growth medium Substances 0.000 claims abstract description 10
- 150000001875 compounds Chemical class 0.000 claims abstract description 8
- 238000011081 inoculation Methods 0.000 claims abstract description 6
- 244000062804 prey Species 0.000 claims abstract description 6
- 238000005286 illumination Methods 0.000 claims abstract description 5
- 238000003892 spreading Methods 0.000 claims abstract description 4
- 241000196324 Embryophyta Species 0.000 claims description 27
- 241000219977 Vigna Species 0.000 claims description 24
- 235000010726 Vigna sinensis Nutrition 0.000 claims description 22
- 241000238876 Acari Species 0.000 claims description 15
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 14
- 239000012153 distilled water Substances 0.000 claims description 6
- 238000011010 flushing procedure Methods 0.000 claims description 6
- 235000013305 food Nutrition 0.000 claims description 4
- 238000002791 soaking Methods 0.000 claims description 4
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 3
- QSHDDOUJBYECFT-UHFFFAOYSA-N mercury Chemical compound [Hg] QSHDDOUJBYECFT-UHFFFAOYSA-N 0.000 claims description 3
- 229910052753 mercury Inorganic materials 0.000 claims description 3
- 238000009331 sowing Methods 0.000 claims description 2
- 238000012136 culture method Methods 0.000 claims 1
- 238000002955 isolation Methods 0.000 claims 1
- 238000000034 method Methods 0.000 abstract description 4
- 238000012258 culturing Methods 0.000 abstract description 3
- 230000007774 longterm Effects 0.000 abstract description 2
- 241000607479 Yersinia pestis Species 0.000 description 4
- 241001658757 Neoseiulus californicus Species 0.000 description 3
- 241000819999 Nymphes Species 0.000 description 2
- 241001454295 Tetranychidae Species 0.000 description 2
- 241000344246 Tetranychus cinnabarinus Species 0.000 description 2
- 238000012271 agricultural production Methods 0.000 description 2
- 230000003247 decreasing effect Effects 0.000 description 2
- 239000010451 perlite Substances 0.000 description 2
- 235000019362 perlite Nutrition 0.000 description 2
- 239000010455 vermiculite Substances 0.000 description 2
- 235000019354 vermiculite Nutrition 0.000 description 2
- 229910052902 vermiculite Inorganic materials 0.000 description 2
- 241001619980 Acaphylla theae Species 0.000 description 1
- 241000207199 Citrus Species 0.000 description 1
- 101100186820 Drosophila melanogaster sicily gene Proteins 0.000 description 1
- 241000488562 Eotetranychus Species 0.000 description 1
- 235000016623 Fragaria vesca Nutrition 0.000 description 1
- 240000009088 Fragaria x ananassa Species 0.000 description 1
- 235000011363 Fragaria x ananassa Nutrition 0.000 description 1
- 240000003183 Manihot esculenta Species 0.000 description 1
- 235000016735 Manihot esculenta subsp esculenta Nutrition 0.000 description 1
- 241000488581 Panonychus citri Species 0.000 description 1
- 241000488583 Panonychus ulmi Species 0.000 description 1
- 244000025272 Persea americana Species 0.000 description 1
- 235000008673 Persea americana Nutrition 0.000 description 1
- 241001481699 Phytoseiidae Species 0.000 description 1
- 241000952063 Polyphagotarsonemus latus Species 0.000 description 1
- 241000344254 Tetranychus truncatus Species 0.000 description 1
- 241000219094 Vitaceae Species 0.000 description 1
- 240000008042 Zea mays Species 0.000 description 1
- 235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 description 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 1
- 230000006978 adaptation Effects 0.000 description 1
- 235000019789 appetite Nutrition 0.000 description 1
- 230000036528 appetite Effects 0.000 description 1
- 239000012681 biocontrol agent Substances 0.000 description 1
- 235000020971 citrus fruits Nutrition 0.000 description 1
- 238000005520 cutting process Methods 0.000 description 1
- -1 dult Species 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 235000013601 eggs Nutrition 0.000 description 1
- YYJNOYZRYGDPNH-MFKUBSTISA-N fenpyroximate Chemical compound C=1C=C(C(=O)OC(C)(C)C)C=CC=1CO/N=C/C=1C(C)=NN(C)C=1OC1=CC=CC=C1 YYJNOYZRYGDPNH-MFKUBSTISA-N 0.000 description 1
- 235000021021 grapes Nutrition 0.000 description 1
- 235000009973 maize Nutrition 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 239000000575 pesticide Substances 0.000 description 1
- 239000000447 pesticide residue Substances 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/10—Animals; Substances produced thereby or obtained therefrom
- A01N63/16—Arachnids
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Catching Or Destruction (AREA)
Abstract
Austracy
The present invention provides a large-scale artificial breeding method for
Neoseiulu californicus of a drought-enduring strain. The method includes the
following steps: building a plant culture room, a prey breeding room and a predatory
mite breeding room isolated from one another; placing an automatic thermostatic
glass culture box in each room, and maintaining a temperature to be 25°C1°C,
wherein relative humidity is 40-50%, an illumination period is 16L:8D, and trays are
placed in the culture boxes; placing 16 incubators in the trays, and spreading culture
media of 5-7 cm thick in the incubators; culturing prey host plants in the glass
culture box of the plant culture room, and germinating the prey host plants and
enabling the plants to grow a first compound leaf for later use; transferring the
cultured prey host plants into the prey breeding room, and inoculating Tetranychus
turkestani for breeding the preys; transferring the culture trays into the predatory
mite breeding room for performing inoculation breeding on the Neoseiulu
californicus within about 15 days after the Tetranychus turkestani is inoculated to
the prey host plants; and collecting the Neoseiulu californicus within 10-15 days
after the Neoseiulu californicus is inoculated. Therefore, long-term supply of the
Neoseiulu californicus may be ensured.
1
Description
Austracy
The present invention provides a large-scale artificial breeding method for Neoseiulu californicus of a drought-enduring strain. The method includes the following steps: building a plant culture room, a prey breeding room and a predatory mite breeding room isolated from one another; placing an automatic thermostatic glass culture box in each room, and maintaining a temperature to be 25°C1°C, wherein relative humidity is 40-50%, an illumination period is 16L:8D, and trays are placed in the culture boxes; placing 16 incubators in the trays, and spreading culture media of 5-7 cm thick in the incubators; culturing prey host plants in the glass culture box of the plant culture room, and germinating the prey host plants and enabling the plants to grow a first compound leaf for later use; transferring the cultured prey host plants into the prey breeding room, and inoculating Tetranychus turkestani for breeding the preys; transferring the culture trays into the predatory mite breeding room for performing inoculation breeding on the Neoseiulu californicus within about 15 days after the Tetranychus turkestani is inoculated to the prey host plants; and collecting the Neoseiulu californicus within 10-15 days after the Neoseiulu californicus is inoculated. Therefore, long-term supply of the Neoseiulu californicus may be ensured.
Description
Technical Field
The present invention belongs to the field of biological control of agricultural pests, and particularly relates to a large-scale artificial breeding method for Neoseiulu ca/fornicus of a drought-enduring strain.
Background
Neoseiulu ca/ifornicus (McGregor) belongs to Neoseiulu of Phytoseiidae in Acari. The natural population of the Neoseiulu cahifornicus is widely distributed in Argentina, Chile, California, Florida, Texas, Japan, South Africa, southern European region and the Mediterranean coast. The Neoseiulu caifornicus is extensively perched on multiple crops such as avocado, citrus and other fruit trees, as well as cassava, maize, grapes, strawberry, and some vegetable crops and ornamental plants, and is an important natural enemy that has an excellent natural control effect on pest mites.
The Neoseiulu cahifornicus is wide in scope of predation and can catch and feed on multiple pest mites that mainly include Tetranychus cinnabarinus, Tetranychus truncatus Ehara, Panonychus citri Me Gregor, Eotetranychus kankitus, Polyphagotarsonemus latus Banks, Panonychus Ulmi and Acaphylla theae.
There are many commercial predatory mites in the current market, but it is difficult to control agricultural spider mites on rain fed crops. The Neoseiulu cahifornicus is wide in humidity adaptation range (relative humidity of 25-90%). Through intraspecific variation caused by humidity sensibility, drought-enduring strains of the Neoseiulu ca/fornicus can be screened. A. Walzer, P. Schausberger
Description
(2008)1 et al. and E. Palevsky (2010)2 et al. researched survival rates, development and reproduction of the Neoseiulu californicus of eight different strains (FR of a Herault producing area in France, FL of a Tuscany producing area in Italy, SP of a Valencia producing area in Spain, CH of a LaCruz producing area in Chile, SI of a Sicily producing area in Italy and C-BOKU, C-ISZA and C-ARO in California). It was discovered that, the strain BOKU was the drought-enduring strain of the Neoseiulu californicus that is the most applicable to an arid environment.
The N. californicus has been taken as a very effective biocontrol agent for controlling red spider mites in foreign countries. Due to short developmental duration, high reproduction speed, long service life of female adult mites, wide scope of predation and great appetite, the N. californicus is used for biologically controlling the pest mites, thereby significantly decreasing consumption of chemical pesticides, decreasing pesticide residues in agricultural products and improving yield and quality of the agricultural products. Controlling the agricultural spider mites by using the artificially bred N. californicusis a biological control method friendly to the ecological environment. The N. californicus has wide application prospects in agricultural production, particularly production of green organic agricultural products.
Summary
A purpose of the present invention is to provide a large-scale artificial breeding method for Neoseiulu californicus of a drought-enduring strain. The method can ensure that a food source needed by breeding of the Neoseiulu californicus can be continuously supplied to produce lots of Neoseiulu californicus for a long time, thereby meeting agricultural production needs. The present invention is realized as follows: the large-scale artificial breeding method for Neoseiulu californicus includes the following steps: building a plant culture room, a prey breeding room and a predatory mite breeding room isolated
Description
from one another, wherein peripheries of the rooms are isolated by water tanks, and the water tanks are periodically flushed for preventing escape of prey and predatory mites; maintaining a temperature to be 25°C1°C, wherein humidity is -50%; an illumination period is 16L:8D; temperature, light and humidity control appliances include computers, air conditioners, lighting equipment, humidifiers, temperature and humidity sensors, automatic thermostatic glass culture boxes and refrigerated cabinets; and trays are placed in the glass culture boxes; placing 16 incubators in each tray, and spreading culture media of 5-7 cm thick in the incubators; culturing prey host plants in the incubators of the plant culture room, and germinating the prey host plants and enabling the plants to grow a first compound leaf for later use; transferring the culture trays of the prey host plants into the prey breeding room, and inoculating Tetranychus turkestani for breeding the preys; transferring the culture trays into the predatory mite breeding room for performing inoculation breeding on the Neoseiulu californicus within about 15 days after the Tetranychus turkestani is inoculated to the prey host plants; and collecting the Neoseiulu calfornicus within 10-15 days after the Neoseiulu calfornicus is inoculated. The food-derived host plant is cowpea; and culture of the prey host plants specifically includes: flushing cowpea seeds with running water for 10 minutes; soaking the seeds with alcohol for 30 s; flushing the seeds with distilled water twice; soaking the seeds with 0.1% of mercury arsenide for 8-10 minutes; flushing the seeds with the distilled water for three times; sowing 5-8 cowpea seeds in each incubator, wherein a depth of the cowpea seeds is 1-2 cm; maintaining humidity of a culture medium in the incubator; and germinating the seeds and enabling the seeds to grow a first compound leaf within 7-10 days for later use. Each incubator is a 10cmx10cm black nutrition package; and the culture medium is prepared from vermiculite and perlite according to a ratio of 1:1 and sterilized at a high temperature. Breeding of the preys specifically includes: transferring the cultured host plants into the prey culture room; placing leaves with Tetranychus turkestani on
Description
the cowpea leaves; and growing and reproducing the Tetranychus turkestani for later use. The inoculation breeding of the Neoseiulu cahfornicus specifically includes: transferring the culture trays into the predatory mite breeding room when the total adult mites and nymph mites on the cowpea leaves reproduce to averagely 150-300 mites per leaf within about 15 days after the Tetranychus turkestani is inoculated onto the cowpea leaves; cutting the leaves with the Neoseiulu cahfornicus into small pieces; placing the small pieces on the leaves of cowpea plants bred with the Tetranychus turkestani, wherein the number of the Neoseiulu cahfornicus adults on each cowpea leaf is 5-10; and collecting the Neoseiulu cafornicus within -20 days after the Neoseiulu cahfornicus is inoculated. In the present invention, the vermiculite and perlite are taken as the culture media and can be recycled after sterilization; the cowpea serves as the prey host plant and is easy to culture; and the Tetranychus turkestani serves as the prey, and then serves as a food source of the Neoseiulu cahfornicus.
Detailed Description
Embodiments of the present invention: an artificial breeding method for Neoseiulu californicus includes the following steps: a plant culture room, a prey breeding room and a predatory mite breeding room isolated from one another were built, wherein peripheries of the prey breeding room and the predatory mite breeding room were isolated by water tanks; automatic thermostatic glass culture boxes were placed in the rooms; a temperature was maintained to be 25°C1°C, wherein humidity was 40-50%; an illumination period was 16L:8D; trays were placed in the glass culture boxes; 16 incubators were placed in each tray, and culture media of 6-7 cm thick were spread in the incubators; cowpeas were cultured in the incubators; specifically, cowpea seeds were flushed with running water for 10 minutes; the seeds were soaked with alcohol for 30 s; the seeds were flushed with distilled water twice; the seeds were soaked with 0.1% of mercury
Description
arsenide for 8-10 minutes; the seeds were flushed with the distilled water for three times; 5-8 cowpea seeds were sowed in each incubator, wherein a depth of the cowpea seeds was 1-2 cm; humidity of a culture medium in the incubator was maintained; and the seeds were germinated and enabled to grow a first compound leaf within 7-10 days for later use; the cultured cowpea seedlings were transferred into the prey breeding room, and Tetranychus turkestani was inoculated for breeding the preys; specifically, when the first compound leaf of the cowpea grew, the cowpea seedlings were transferred into the prey breeding room; cowpea leaves containing pure leaf mites were inoculated; then the leaf mites automatically crawled up the host plants to randomly grow and reproduce on the plants; lots of leaf mite eggs, nymph mites and adult mites are reproduced on the back of the leaves within about half a month; then the culture trays were transferred into the predatory mite breeding room for performing inoculation breeding on the Neoseiulu californicus; specifically, the culture trays were transferred into the predatory mite breeding room; the leaves with the Neoseiulu californicus were cut into small pieces; the small pieces were placed on the leaves of cowpea plants fed with the Tetranychus turkestani, wherein the number of the Neoseiulu californicus adults on each cowpea leaf was 5; and the Neoseiulu californicus was collected within 15-20 days after the Neoseiulu californicus was inoculated.
Claims (5)
1. A large-scale artificial breeding method for Neoseiulu californicus of a drought-enduring strain, comprising: building a plant culture room, a prey breeding room and a predatory mite breeding room isolated from one another; and ensuring that a food source needed by breeding of the Neoseiulu californicus is continuously supplied to produce lots of Neoseiulu californicusin batches.
2. The large-scale artificial breeding method for Neoseiulu californicus according to claim 1, wherein specific isolation measures are as follows: peripheries of the prey breeding room and the predatory mite breeding room are isolated by water tanks, and the water tanks are periodically flushed for preventing escape of prey and predatory mites.
3. The large-scale artificial breeding method for Neoseiulu californicus according to claim 1, wherein a temperature of each room is maintained to be °C1C; relative humidity is 40-50%; an illumination period is 16L:8D; and glass culture boxes are placed in the rooms.
4. The large-scale artificial breeding method for Neoseiulu californicus according to claim 1, wherein a plant cultured in the plant culture room is cowpea; and a culture method comprises steps: placing trays on the glass culture boxes; placing 16 incubators on each tray; spreading culture media of 5-7 cm thick in the incubators; flushing cowpea seeds with running water for 10 minutes; soaking the seeds with alcohol for 30 s; flushing the seeds with distilled water twice; soaking the seeds with 0.1% of mercury arsenide for 8-10 minutes; flushing the seeds with the distilled water for three times; sowing 5-8 cowpea seeds in each incubator, wherein a depth of the cowpea seeds is 1-2 cm; maintaining humidity of a culture medium in the incubator; and germinating the seeds and enabling the seeds to grow a first compound leaf within 7-10 days for later use.
5. The large-scale artificial breeding method for Neoseiulu californicus according to claim 1, further comprising: transferring the culture trays of the prey host plants that grow the first compound leaf into the prey breeding room, and
Claims
inoculating Tetranychus turkestani for breeding the preys; transferring the culture trays into the predatory mite breeding room for performing inoculation breeding on the Neoseiulu californicus within about 15 days after the Tetranychus turkestani is inoculated to the prey host plants; and collecting the Neoseiulu californicus within -15 days after the Neoseiulu californicus is inoculated.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| AU2020102730A AU2020102730A4 (en) | 2020-10-15 | 2020-10-15 | Large-scale artificial breeding method for neoseiulu californicus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| AU2020102730A AU2020102730A4 (en) | 2020-10-15 | 2020-10-15 | Large-scale artificial breeding method for neoseiulu californicus |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AU2020102730A4 true AU2020102730A4 (en) | 2020-12-03 |
Family
ID=73551691
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AU2020102730A Ceased AU2020102730A4 (en) | 2020-10-15 | 2020-10-15 | Large-scale artificial breeding method for neoseiulu californicus |
Country Status (1)
| Country | Link |
|---|---|
| AU (1) | AU2020102730A4 (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112586453A (en) * | 2020-12-05 | 2021-04-02 | 中国农业科学院郑州果树研究所 | Simple feeding method for predatory mites |
| CN115281158A (en) * | 2022-08-15 | 2022-11-04 | 绵阳市农业科学研究院 | Method for efficient large-scale propagation of phytoseiulus persimilis |
-
2020
- 2020-10-15 AU AU2020102730A patent/AU2020102730A4/en not_active Ceased
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112586453A (en) * | 2020-12-05 | 2021-04-02 | 中国农业科学院郑州果树研究所 | Simple feeding method for predatory mites |
| CN115281158A (en) * | 2022-08-15 | 2022-11-04 | 绵阳市农业科学研究院 | Method for efficient large-scale propagation of phytoseiulus persimilis |
| CN115281158B (en) * | 2022-08-15 | 2023-09-15 | 绵阳市农业科学研究院 | Method for large-scale propagation of phytoseiid chile |
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