CN107736251B - Lily lilium test tube bulblet propagation method - Google Patents
Lily lilium test tube bulblet propagation method Download PDFInfo
- Publication number
- CN107736251B CN107736251B CN201711148992.2A CN201711148992A CN107736251B CN 107736251 B CN107736251 B CN 107736251B CN 201711148992 A CN201711148992 A CN 201711148992A CN 107736251 B CN107736251 B CN 107736251B
- Authority
- CN
- China
- Prior art keywords
- bulblet
- bulblets
- culture medium
- scales
- test tube
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Developmental Biology & Embryology (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention discloses a lilium brownii test tube bulblet propagation method, belongs to the field of biology, and aims to provide a lilium brownii test tube bulblet propagation method to solve the problem that seedling growth is slow for lilium brownii propagation by a method commonly used in existing lily tissue culture. The method comprises the steps of disinfection of scale explants, induction of test tube bulblets, expansion and rooting of the test tube bulblets and accelerating germination and growth of the bulblets. The bulblet induction culture medium consists of a basic culture medium and hormone, wherein the basic culture medium consists of MS basic culture solution, 60g/L of cane sugar, 0.5g/L of arginine, 0.5g/L of glutamine, 2g/L of activated carbon, 6g/L of agar, 500mg/L of dithiothreitol and 800 mg/L of butylparaben. The invention has the advantages of high propagation coefficient, short culture period, high efficiency, high transplanting survival rate, convenient transportation, simple and convenient operation and low cost.
Description
Technical Field
The invention belongs to the field of biology, and particularly relates to a method for cultivating lilium brownii test-tube bulblets.
Background
Yichang lily is a special species in China, is mainly distributed in Hubei and Sichuan, has trumpet-shaped flowers, has slight fragrance, can extract essential oil, has higher ornamental value, can be used in the landscaping industry, has the functions of easing pain, diminishing inflammation, drawing out toxin, inhibiting bacteria, resisting oxidation and the like, and can be used for developing functional foods and medicines. The wild lilium brownii resources are mainly distributed along the Qinba mountain areas such as Longnan and Gannan in Gansu province; the lilium brownii is used as a variety of the lilium brownii, has similarity with the lilium brownii in function and application, and is different from the lilium brownii in form that the outer surface of a quilt sheet is purple or brown ridge zone, the altitude of the lilium brownii is 2500m, which is obviously higher than the altitude range of the lilium brownii, which is 450 m and 1500m, so the lilium brownii has stronger cold resistance and drought resistance than the lilium brownii, and the annual growth period and the growth amount are less than the lilium brownii. The special habitat molds the special biological characteristics of lilium brownii, and according to field investigation of penmen and introduction of local farmers: lilium brownii is difficult to reproduce and slow in growth, has a habit of digging and digging up bulbs for viewing and eating in folk, is limited by the reproductive performance, and is not planted in a large scale at present.
The lilium brownii has few reports in documents, but has great significance in systematically researching the lilium brownii as a wild resource with potential huge development value. Regarding the breeding problem of lilium brownii, the pen workers have conducted related studies using the traditional breeding method of lilium brownii and summarized as follows: the lilium brownii is mainly subjected to seed propagation and scale cutting propagation, and no plant division propagation and plant bud propagation are observed. The lilium brownii has dual dormancy of shape and physiology, low seed germination rate (the germination rate in the first growth period is about 10 percent, the germination rate in the second growth period is about 50 percent), slow seedling growth and long seedling growing period; the scale cuttage propagation is affected by high content of secondary metabolites, the propagation efficiency is low (the outer-layer scales are seriously infected with bacteria, the inner-layer scales are not easy to differentiate, only the middle-layer scales are differentiated into 1-2 bulblets), the period is long (the scale matrix cuttage can begin to differentiate the bulblets after 6 months of dormancy, and the scale field spring cuttage and summer cuttage need to wait until autumn or spring of the next year to grow).
The commonly used method for lily tissue culture is to culture lily seedlings by means of adventitious bud or somatic embryo induction, so as to promote seedling rooting and bulblet expansion, and the method has the disadvantages that the seedlings and the leaves consume a large amount of nutrition, labor and time are wasted, the cost is increased, and the method is limited by the problems of illumination intensity and carbon dioxide concentration under the tissue culture condition, the photosynthetic efficiency of the leaves generated in the tissue culture is very low, part of the leaves wither during seedling exercising and transplanting, new leaves need to be grown again, and the nutrition of the bulblet is consumed, so that the seedlings grow slowly, and therefore, the method is not an efficient culture strategy.
Disclosure of Invention
The invention aims to provide a lilium brownii test tube bulblet propagation method, which solves the problem that the seedling growth is slow for lilium brownii propagation by the commonly used method for lily tissue culture in the prior art.
The technical scheme of the invention is as follows: a lilium brownii test tube bulblet propagation method comprises the following steps:
(1) disinfection of scale explants
Digging a flowering adult lilium brownii bulb, removing the outermost layer of scales with disease spots, washing, breaking off the outer layer and middle layer of scales along a bulb disc, soaking, washing, cutting off 1/3 parts of tips of the scales, and longitudinally cutting the rest scales into strips of 1.5-2 cm for later use;
(2) induction of test tube bulblets
Inoculating the above scales as explants in bulblet induction culture medium, culturing in dark at 25-27 deg.C,
the bulblet induction culture medium consists of a basic culture medium and hormone, wherein the basic culture medium consists of MS basic culture solution, 60g/L of cane sugar, 0.5g/L of arginine, 0.5g/L of glutamine, 2g/L of active carbon, 6g/L of agar, 500mg/L of dithiothreitol and 800 mg/L of butylparaben;
the hormones consisted of: indole acetic acid 3mg/L, cytokinin 3mg/L, brassinolide 0.3-0.7 mg/L, methyl jasmonate 5-13 mg/L;
(3) enlargement and rooting of test tube bulblets
Transferring the scales with the bulblets into a bulblet expansion and rooting culture medium, and carrying out dark culture;
(4) accelerating germination and growth of bulblet
And taking the rooted bulblets out of the culture medium, washing the rooted bulblets clean by running water, sowing the rooted bulblets in the mixed matrix, and growing the rooted bulblets under natural conditions.
Preferably, in the step of disinfecting the scale explants: soaking the scales in the outer layer and the middle layer in 0.2% mercuric chloride for 15-20 min.
Preferably, the cytokinin of step (2) is trans-zeatin or 6-benzyladenine.
Preferably, the concentration of the butylparaben in the step (2) is 600 mg/L.
Preferably, the concentration of the brassinolide in the step (2) is 0.5mg/L,
preferably, the concentration of the methyl jasmonate in the step (2) is 8 mg/L.
Preferably, the bulblet expansion and rooting culture medium in the step (3) is composed of a minimal medium and hormones, wherein the minimal medium is composed of: MS basic culture solution, 60g/L of cane sugar, 1g/L of acid hydrolyzed casein, 0.5g/L of arginine, 0.5g/L of glutamine, 2g/L of activated carbon and 6g/L of agar, wherein the hormone is indoleacetic acid 0.5mg/L, strigolactone 0.3-0.8mg/L and plant growth regulator 5-20 mg/L.
Preferably, the plant growth regulator is uniconazole or methyl jasmonate.
Preferably, the concentration of the strigolactone is 0.5mg/L, the concentration of the uniconazole is 8mg/L, and the concentration of the methyl jasmonate is 20 mg/L.
Preferably, in the step of accelerating the germination and growing of the bulblets, the rooted bulblets are soaked in 3-10 mg/L karrikinolide solution for 60 minutes, and the volume ratio of the mixed matrix is 1:1 vermiculite and perlite.
The components MS basic culture solution and 60g/L sucrose in the bulblet induction basic culture medium provide basic mineral nutrition and carbon source for the culture of explant materials; the addition of 0.5g/L arginine and 0.5g/L glutamine increases the content of organic amine in the culture medium, and is beneficial to the formation of bulblet; the addition of 2g/L of activated carbon not only provides a dark environment, but also can adsorb harmful substances such as quinones and the like generated in the culture process, the addition of 500mg/L of dithiothreitol can effectively inhibit browning, and the aim of inducing test tube bulblet can be finally achieved by adding butylparaben and different hormones on the basis of the basic conditions. The butyl paraben has the beneficial effects of obviously reducing the contamination rate of the explant, inhibiting the growth of endophytic fungi of the explant and having no obvious inhibiting effect on the growth and development of the explant.
Scale explants were cultured in bulblet induction medium for 25 days, tube bulblets started to occur, and at 60 days an average of 2-3 tube bulblets per explant were produced, 0.5-0.8 cm in diameter, and started to have a tendency to germinate and leave. The test tube bulblet directly takes place on the scale, which is different from the way that adventitious bud and somatic embryo approach firstly leaves and then the base expands to generate bulblet.
The components in the basic culture medium for the expansion and rooting of the bulblets, namely MS basic culture solution and 60g/L sucrose, provide basic mineral nutrition and carbon source for the expansion and rooting of the bulblets; the addition of 1g/L acid hydrolyzed casein, 0.5g/L arginine and 0.5g/L glutamine increases the content of micromolecule amino acid in the culture medium, and the addition of micromolecule organic nitrogen is beneficial to the growth and expansion of lilium brownii which is a nitrogen-rich crop; the addition of 2g/L activated carbon not only provides a dark environment, but also can adsorb harmful substances such as quinones generated in the culture process. On the basis, the purpose of promoting the expansion and rooting of the bulblets can be achieved by adding different hormones.
The addition of the strigolactone GR24 and the uniconazole S-3307 or the addition of the methyl jasmonate MEJA in the bulblet expansion and rooting basal medium effectively inhibits the growth of leaves, reduces the consumption of nutrition of the bulblet by the growth of the leaves with low photosynthetic efficiency in the tissue culture process, does not influence the generation of leaf buds, promotes the rooting and expansion of the bulblet, inhibits the generation of a multiheaded phenomenon, and provides guarantee for improving the transplanting survival rate and the germination rate of the test-tube bulblet.
The invention provides a method for directly inducing test-tube bulblet generation by an explant, further inducing the bulblet to expand and root and then accelerating germination and transplanting of the rooted bulblet aiming at various difficulties in lilium brownii propagation. The method has the advantages of high propagation coefficient, short culture period, high efficiency, high transplanting survival rate, convenient transportation, simple and convenient operation and low cost, can effectively improve the propagation efficiency from the test tube to the field, and has important significance for lilium violaceum resource protection and seedball production. Lays a solid foundation for the industrialized breeding of lilium brownii.
Detailed Description
The following examples further illustrate the invention but are not intended to limit the invention in any way.
In the examples, the sources of the raw materials are as follows: indoleacetic acid (IAA), Brassinolide (BR), strigolactone (GR 24), methyl jasmonate (MEJA), uniconazole (S-3307), trans-Zeatin (ZT), 6-benzyladenine (6-BA), Dithiothreitol (DTT), butylparaben were purchased from Shanghai-derived leaf Biotech, Inc.; karrikinolide was purchased from carbofuran technologies.
Water-soluble fertilizer (nitrogen phosphorus potassium content ratio is 15-15-30+ microelement TE) is purchased from Moore chemical industry.
The lilium brownii bulbs used in the following experiments are all collected from Shang county of Longnan City at an altitude of more than 1800 m, and are identified as lilium brownii, and the bulbs are flowering lilium brownii and are stored for later use at a fresh or 4 ℃.
Example 1-example 12: induction of bulblet
Firstly, scale explant disinfection: removing the scales with disease spots on the outermost layer of lilium brownii, breaking off the scales on the outer layer and the middle layer along the bulb disc, washing the scales clean with running water, soaking the scales for 15 to 20 minutes with 0.2 percent mercuric chloride, washing the scales clean with sterilized distilled water, cutting off 1/3 parts of the tips of the scales, and longitudinally cutting the remaining scales into strips of 1.5 to 2 centimeters for later use.
Second, induction of test tube bulblet
Taking the scales as explants, inoculating the scales in a bulblet induction culture medium under the dark condition of 25-27 ℃, wherein the bulblet induction culture medium comprises a basic culture medium and is added with different hormones for culture, and the basic culture medium comprises the following components: MS basic culture solution, 60g/L of cane sugar, 0.5g/L of arginine, 0.5g/L of glutamine, 2g/L of activated carbon, 6g/L of agar, 500mg/L of dithiothreitol and butyl paraben, wherein the pH value of the culture medium is 6, and the same culture medium is replaced once when the culture medium is cultured for 60 days in total (30 days). The proportions of the different hormones are shown in table 2.
The dosage of butylparaben for different examples is shown in Table 1, and the concentration of butylparaben in examples 4-12 is 600 mg/L.
The protocol of the example shows that as mercuric chloride soak time is extended, the rate of contamination decreases, but the scale explant activity also decreases.
Bacteriostasis test
The bacteriostatic test and results of butylparaben are shown in Table 1, and the conditions of comparative example 1 are shown in example 1, except that butylparaben is not added.
Test results show that the butylparaben can obviously inhibit infectious microbes, the microbial contamination rate is gradually reduced along with the increase of the concentration of the butylparaben, but the microbial contamination cannot be completely inhibited, and the concentration of the butylparaben of 600mg/L is the optimal concentration in consideration of cost factors.
Test of hormone Effect at different ratios
The test results of the effect of the hormones in different ratios in the induction formula of the test tube bulblet are shown in tables 2-1 and 2-2, the dosage of the hormones in comparative examples 2-5 is shown in tables 2-2, and the rest conditions are the same as in example 1. Note: the data below are the average of 3 replicates.
As can be seen from Table 2, when the concentration ratio of auxin IAA to cytokinin (ZT or 6-BA) is 1:1, test-tube bulblet cannot be induced, but as the time is prolonged, the explant begins to expand before and after 60 days, and there is a sign that callus is generated; at a concentration ratio of 1:10, 50% of explants started to produce adventitious buds around 60 days. When brassinolide BR and methyl jasmonate MEJA are added, the induction of test tube bulblets is effectively promoted, the time required for inducing the bulblet generation tends to be shortened along with the increase of BR concentration, the number of test tube bulblets generated per explant on average tends to increase, but high-concentration BR (0.7mg/L) shows the tendency of inducing callus. With increasing MEJA concentration, there is a tendency for the time required to induce bulblet development to shorten, and on average there is a tendency for the number of tube bulblets produced per explant to increase, but with higher MEJA (13mg/L) concentrations there is an increasing tendency for the explants to brown. Preferably, the optimal formula comprises Indole Acetic Acid (IAA) concentration of 3mg/L, trans-Zeatin (ZT) concentration of 3mg/L, Brassinolide (BR) concentration of 0.5mg/L, methyl jasmonate (MEJA) concentration of 8mg/L, Indole Acetic Acid (IAA) concentration of 3mg/L, 6-benzyladenine (6-BA) concentration of 3mg/L, Brassinolide (BR) concentration of 0.5mg/L, and methyl jasmonate (MEJA) concentration of 8 mg/L.
Example 13-example 22: test tube bulblet expansion and rooting
Transferring the scales with the bulblets into a bulblet expansion and rooting culture medium, culturing in dark at a variable temperature of 22 ℃ for 10 hours/16 ℃ for 14 hours each day, separating the bulblets from the scales at 20 days, transferring into the same culture medium, and continuously culturing for 60 days under the same conditions. At this point, the test tube bulblet roots are approximately 1-3 cm long and approximately 1-1.3 cm in diameter, and the bulblets are carefully harvested for matrix transplantation.
The test tube bulblet expansion and rooting culture medium is formed by adding hormones with different combinations and concentrations into a basic culture medium, and the basic culture medium comprises the following components: MS basic culture solution, 60g/L of cane sugar, 1g/L of acid hydrolyzed casein, 0.5g/L of arginine, 0.5g/L of glutamine, 2g/L of activated carbon, 6g/L of agar and pH value of 6.0. The proportions of the different hormones are shown in table 3.
Experimental on the Effect of enlargement and rooting of bulblet
Table 3 shows the results of the effect of the enlargement and rooting of the bulblets, and the amount of the hormone used in comparative example 6 is shown in Table 3, and the other conditions are the same as in example 13. Note: the data below are the average of 3 replicates.
As can be seen from Table 3, the addition of GR24 and S-3307 in scheme 1, and the addition of GR24 and MEJA in scheme 2 effectively inhibited the growth of leaf blades, but did not affect the occurrence of leaf buds; remarkably promotes the rooting and the expansion of the bulblet and inhibits the occurrence of the multiheaded phenomenon. With increasing GR24 concentration, the time required for rooting tends to decrease, the diameter of the bulblet tends to increase, but the root length tends to decrease. As the concentration of S-3307 increases, there is a tendency for the diameter of the bulblet to increase and for the root length to shorten. As the concentration of MEJA increased, there was a tendency for the root length to shorten, and the use of high concentration MEJA (25 mg/L) showed a tendency for bulblet aging. Overall analysis, each treatment of protocol 2 was longer and stronger than each treatment of protocol 1. In general, the best formulation is 0.5mg/L of Indole Acetic Acid (IAA), 0.5mg/L of strigolactone (GR 24), 8mg/L of uniconazole (S-3307), 0.5mg/L of Indole Acetic Acid (IAA), 0.5mg/L of strigolactone (GR 24) and 20mg/L of methyl jasmonate (MEJA).
Example 23-example 26: accelerating germination and growth of bulblet
Taking out the rooted bulblet root when the root is 2 cm long from the culture medium, washing the rooted bulblet root clean by running water, soaking the rooted bulblet root in 3-10 mg/L karrikinolide solution, wherein the soaking concentration is shown in the table 4, soaking the rooted bulblet root for 60 minutes, and sowing the root in a seed culture medium with a volume ratio of 1:1, placing the mixed substrate of vermiculite and perlite in a natural condition for growing. 1g/L of water-soluble fertilizer is irrigated for 1 time every 7 days, and the type of the water-soluble fertilizer is (15-15-30 + TE). After sowing, leaves grow out about 20 days, and after the seedlings are naturally poured out, the seed balls are harvested for field cultivation in the next year.
Test for growth of bulblet by Germination
The treatment for accelerating the germination and growth of the bulblet and the results are shown in Table 4, and comparative example 7 was carried out without adding the karrikinolide solution under the same conditions as in example 23.
As can be seen from Table 4, the sprouting time of the bulblets was advanced with the increase in the concentration of Karrikinolide, and the difference in the promoting effects was insignificant with the further increase in the concentration of Karrikinolide (8 mg/L and 10 mg/L). However, Karrikinolide has a remarkable effect of promoting the sprouting and leaf growth of the bulblet compared with the comparative example. The application of the water-soluble fertilizer provides basic nutritional conditions for the culture of the matrix.
Claims (6)
1. A lilium brownii test tube bulblet propagation method is characterized by comprising the following steps:
(1) disinfection of scale explants
Digging a flowering adult lilium brownii bulb, removing the outermost layer of scales with disease spots, washing, breaking off the outer layer and middle layer of scales along a bulb disc, soaking, washing, cutting off 1/3 parts of tips of the scales, and longitudinally cutting the rest scales into strips of 1.5-2 cm for later use;
(2) induction of test tube bulblets
Inoculating the above scales as explants in bulblet induction culture medium, culturing in dark at 25-27 deg.C,
the bulblet induction culture medium consists of a basic culture medium and hormone, wherein the basic culture medium consists of MS basic culture solution, 60g/L of cane sugar, 0.5g/L of arginine, 0.5g/L of glutamine, 2g/L of active carbon, 6g/L of agar, 500mg/L of dithiothreitol and 800 mg/L of butylparaben;
the hormones consisted of: indole acetic acid 3mg/L, cytokinin 3mg/L, brassinolide 0.5mg/L, methyl jasmonate 8 mg/L;
(3) enlargement and rooting of test tube bulblets
Transferring the scales with the bulblets into a bulblet expansion and rooting culture medium, and carrying out dark culture;
the bulblet expansion and rooting culture medium consists of a basic culture medium and hormone, wherein the basic culture medium comprises the following components: MS basic culture solution, 60g/L of cane sugar, 1g/L of acid hydrolyzed casein, 0.5g/L of arginine, 0.5g/L of glutamine, 2g/L of activated carbon and 6g/L of agar, wherein the hormone is indoleacetic acid 0.5mg/L, strigolactone 0.3-0.8mg/L and plant growth regulator 5-20 mg/L;
the plant growth regulator is uniconazole or methyl jasmonate;
(4) accelerating germination and growth of bulblet
And taking the rooted bulblets out of the culture medium, washing the rooted bulblets clean by running water, sowing the rooted bulblets in the mixed matrix, and growing the rooted bulblets under natural conditions.
2. The lilium violaceum test-tube bulblet propagation method according to claim 1, characterized in that: the step of disinfecting the scale explants comprises the following steps: soaking the scales in the outer layer and the middle layer in 0.2% mercuric chloride for 15-20 min.
3. The lilium violaceum test-tube bulblet propagation method according to claim 1 or 2, characterized in that: the cytokinin is trans-zeatin or 6-benzyladenine.
4. The lilium brownii test-tube bulblet propagation method according to claim 3, characterized in that: the concentration of the butylparaben in the step (2) is 600 mg/L.
5. The lilium violaceum test-tube bulblet propagation method according to claim 1, characterized in that: the concentration of the strigolactone is 0.5mg/L, the concentration of the uniconazole is 8mg/L, and the concentration of the methyl jasmonate is 20 mg/L.
6. The lilium brownii test-tube bulblet propagation method according to claim 5, characterized in that: in the step of accelerating the sprouting and growing of the bulblets, the rooted bulblets are soaked in 3-10 mg/L karrikinolide solution for 60 minutes, and the volume ratio of a mixed matrix is 1:1 vermiculite and perlite.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711148992.2A CN107736251B (en) | 2017-11-17 | 2017-11-17 | Lily lilium test tube bulblet propagation method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711148992.2A CN107736251B (en) | 2017-11-17 | 2017-11-17 | Lily lilium test tube bulblet propagation method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN107736251A CN107736251A (en) | 2018-02-27 |
| CN107736251B true CN107736251B (en) | 2019-12-20 |
Family
ID=61234899
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711148992.2A Active CN107736251B (en) | 2017-11-17 | 2017-11-17 | Lily lilium test tube bulblet propagation method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107736251B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2022081767A1 (en) * | 2020-10-13 | 2022-04-21 | Phytotech Labs, Inc. | Methods and compositions for axillary shoot micropropagation of cannabis and related plants |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109601540A (en) * | 2018-12-12 | 2019-04-12 | 四川国光农化股份有限公司 | It is a kind of for promoting the composition, preparation and its application of plant root tuber tuber growth |
| CN109380227A (en) * | 2018-12-12 | 2019-02-26 | 四川国光农化股份有限公司 | It is a kind of for promoting the composition, preparation and its application of plant root tuber tuber growth |
| CN110367125A (en) * | 2019-09-03 | 2019-10-25 | 南通大学 | The method and method for resuscitation of lily bulb preservation effect are improved with thermal stimulus |
| CN114711128A (en) * | 2022-03-28 | 2022-07-08 | 北京林业大学 | Water culture method for promoting quick and massive rooting of hyacinth seedballs |
| CN115500263B (en) * | 2022-10-11 | 2023-05-02 | 北京市农林科学院 | Method for rapidly inducing lily scales and expanding test tube bulblets |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103598099A (en) * | 2013-11-27 | 2014-02-26 | 成都大学 | Cultural method for bulbus fritillariae cirrhosae multiploid callus capable of inhibiting brown stain |
| CN104686337A (en) * | 2015-02-22 | 2015-06-10 | 梁仕华 | Tissue culture rapid propagation method of lilium |
| CN104957037A (en) * | 2015-07-07 | 2015-10-07 | 甘肃省科学院生物研究所 | Method for cultivating fritillaria przewalskii test-tube bulblet |
-
2017
- 2017-11-17 CN CN201711148992.2A patent/CN107736251B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103598099A (en) * | 2013-11-27 | 2014-02-26 | 成都大学 | Cultural method for bulbus fritillariae cirrhosae multiploid callus capable of inhibiting brown stain |
| CN104686337A (en) * | 2015-02-22 | 2015-06-10 | 梁仕华 | Tissue culture rapid propagation method of lilium |
| CN104957037A (en) * | 2015-07-07 | 2015-10-07 | 甘肃省科学院生物研究所 | Method for cultivating fritillaria przewalskii test-tube bulblet |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2022081767A1 (en) * | 2020-10-13 | 2022-04-21 | Phytotech Labs, Inc. | Methods and compositions for axillary shoot micropropagation of cannabis and related plants |
Also Published As
| Publication number | Publication date |
|---|---|
| CN107736251A (en) | 2018-02-27 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN107736251B (en) | Lily lilium test tube bulblet propagation method | |
| CN103651121B (en) | A kind of bletilla differentiation, strong seedling culture base | |
| CN104365482B (en) | Miniature Chinese rose tissue culture breeding method | |
| Zeng et al. | Asymbiotic seed germination, induction of calli and protocorm-like bodies, and in vitro seedling development of the rare and endangered Nothodoritis zhejiangensis Chinese orchid | |
| CN105028209B (en) | A kind of method for improving Vaccinium ashei tissue culture seedling rooting | |
| CN103651122B (en) | A kind of bletilla protocorm induction medium | |
| CN104106468B (en) | The quick breeding method for tissue culture of a kind of radix fici simplicissimae | |
| CN101347099B (en) | Method for quickly breeding Louisiana cypress in-vitro | |
| CN102845313A (en) | Method for quickly in-vitro actinidia kolomikta propagating | |
| CN102612961A (en) | Method for cultivating olive seedlings | |
| CN105191792B (en) | The rapid propagation method of almond ringdove chrysanthemum | |
| CN101622955B (en) | Culture medium composition suitable for germ-free germination of orchid seeds and method thereof | |
| CN102919133A (en) | Technical method for promoting tissue culture rooting of alpine rose amethyst | |
| WO2023155834A1 (en) | Use of 2-amino-3-hydroxy-3-methylbutyric acid in promoting plant growth | |
| KR101204896B1 (en) | Tissue Culture Method of Smilax China | |
| CN103155869A (en) | Sweet cherry rootstock Colt tissue culture method | |
| CN107549018A (en) | Chinese mugwort tissue culture method | |
| CN110024688B (en) | Method for caragana germ proliferation and plant regeneration and culture medium thereof | |
| WO2023155855A1 (en) | Application of 2-amino-3-indole butyric acid in promoting plant growth | |
| CN101965798B (en) | Peanut somatic embryo induction and plant regeneration method | |
| CN109247235B (en) | Rapid breeding and seedling raising method for cymbidium faberi Rolfe | |
| Kane et al. | In vitro propagation of Cryptocoryne wendtii | |
| CN1157107C (en) | Fast propagating method of Dendrobium nobile Lindl | |
| CN105519434A (en) | A tissue cultured seedling micrografting method for acer palmatum 'orange dream' | |
| CN109156350A (en) | A kind of method of the numerous bud of wind resistance paulownia and root media and promotion wind resistance paulownia Vitro Quick Reproduction |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20201210 Address after: Hongdakou, huazansi Town, Tianzhu Tibetan Autonomous County, Wuwei City, Gansu Province Patentee after: GANSU LVNENG AGRICULTURAL SCIENCE AND TECHNOLOGY Co.,Ltd. Address before: 730000 No. 197 South Dingxi Road, Chengguan District, Gansu, Lanzhou Patentee before: Institute of Biology, Gansu Academy of Sciences |