AU2017341798B2 - Biosynthetic production of steviol glycosides and processes therefore - Google Patents

Biosynthetic production of steviol glycosides and processes therefore Download PDF

Info

Publication number
AU2017341798B2
AU2017341798B2 AU2017341798A AU2017341798A AU2017341798B2 AU 2017341798 B2 AU2017341798 B2 AU 2017341798B2 AU 2017341798 A AU2017341798 A AU 2017341798A AU 2017341798 A AU2017341798 A AU 2017341798A AU 2017341798 B2 AU2017341798 B2 AU 2017341798B2
Authority
AU
Australia
Prior art keywords
rebaudioside
reb
leu
ser
glu
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
AU2017341798A
Other languages
English (en)
Other versions
AU2017341798A1 (en
Inventor
Michael Batten
David BYUN
Yang Luo
Guohong MAO
Jacob Edward VICK
Yilin Wu
Xiaodan Yu
Beihua ZHANG
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Conagen Inc
Original Assignee
Conagen Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Conagen Inc filed Critical Conagen Inc
Publication of AU2017341798A1 publication Critical patent/AU2017341798A1/en
Application granted granted Critical
Publication of AU2017341798B2 publication Critical patent/AU2017341798B2/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/44—Preparation of O-glycosides, e.g. glucosides
    • C12P19/56—Preparation of O-glycosides, e.g. glucosides having an oxygen atom of the saccharide radical directly bound to a condensed ring system having three or more carbocyclic rings, e.g. daunomycin, adriamycin
    • A—HUMAN NECESSITIES
    • A21—BAKING; EDIBLE DOUGHS
    • A21D—TREATMENT OF FLOUR OR DOUGH FOR BAKING, e.g. BY ADDITION OF MATERIALS; BAKING; BAKERY PRODUCTS
    • A21D13/00—Finished or partly finished bakery products
    • A21D13/06—Products with modified nutritive value, e.g. with modified starch content
    • A21D13/062—Products with modified nutritive value, e.g. with modified starch content with modified sugar content; Sugar-free products
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23G—COCOA; COCOA PRODUCTS, e.g. CHOCOLATE; SUBSTITUTES FOR COCOA OR COCOA PRODUCTS; CONFECTIONERY; CHEWING GUM; ICE-CREAM; PREPARATION THEREOF
    • A23G4/00—Chewing gum
    • A23G4/06—Chewing gum characterised by the composition containing organic or inorganic compounds
    • A23G4/10—Chewing gum characterised by the composition containing organic or inorganic compounds characterised by the carbohydrates used, e.g. polysaccharides
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Preparation or treatment thereof
    • A23L2/52—Adding ingredients
    • A23L2/60—Sweeteners
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L27/00—Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
    • A23L27/30—Artificial sweetening agents
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L27/00—Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
    • A23L27/30—Artificial sweetening agents
    • A23L27/33—Artificial sweetening agents containing sugars or derivatives
    • A23L27/36—Terpene glycosides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H1/00—Processes for the preparation of sugar derivatives
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H15/00—Compounds containing hydrocarbon or substituted hydrocarbon radicals directly attached to hetero atoms of saccharide radicals
    • C07H15/20—Carbocyclic rings
    • C07H15/24—Condensed ring systems having three or more rings
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H15/00—Compounds containing hydrocarbon or substituted hydrocarbon radicals directly attached to hetero atoms of saccharide radicals
    • C07H15/20—Carbocyclic rings
    • C07H15/24—Condensed ring systems having three or more rings
    • C07H15/256—Polyterpene radicals
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8245—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C12N9/1062—Sucrose synthase (2.4.1.13)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1081—Glycosyltransferases (2.4) transferring other glycosyl groups (2.4.99)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2445—Beta-glucosidase (3.2.1.21)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/18—Preparation of compounds containing saccharide radicals produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/44—Preparation of O-glycosides, e.g. glucosides
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/163—Sugars; Polysaccharides
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C12Y204/01013—Sucrose synthase (2.4.1.13)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C12Y204/01028—Glucosyl-DNA beta-glucosyltransferase (2.4.1.28)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01021—Beta-glucosidase (3.2.1.21)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Nutrition Science (AREA)
  • Food Science & Technology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Polymers & Plastics (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Mycology (AREA)
  • Inorganic Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicinal Preparation (AREA)
AU2017341798A 2016-10-14 2017-10-13 Biosynthetic production of steviol glycosides and processes therefore Active AU2017341798B2 (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201662408179P 2016-10-14 2016-10-14
US62/408,179 2016-10-14
US201762555809P 2017-09-08 2017-09-08
US62/555,809 2017-09-08
PCT/US2017/056457 WO2018071744A1 (en) 2016-10-14 2017-10-13 Biosynthetic production of steviol glycosides and processes therefore

Publications (2)

Publication Number Publication Date
AU2017341798A1 AU2017341798A1 (en) 2019-04-18
AU2017341798B2 true AU2017341798B2 (en) 2021-10-21

Family

ID=61906334

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2017341798A Active AU2017341798B2 (en) 2016-10-14 2017-10-13 Biosynthetic production of steviol glycosides and processes therefore

Country Status (13)

Country Link
US (4) US11098315B2 (enExample)
EP (1) EP3525598B1 (enExample)
JP (2) JP7177048B2 (enExample)
KR (1) KR102540615B1 (enExample)
CN (1) CN110191643B (enExample)
AU (1) AU2017341798B2 (enExample)
BR (1) BR112019007504B1 (enExample)
CA (1) CA3039105C (enExample)
ES (1) ES2964976T3 (enExample)
HU (1) HUE064754T2 (enExample)
MX (1) MX2019004368A (enExample)
RU (1) RU2019114181A (enExample)
WO (1) WO2018071744A1 (enExample)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2015327940B2 (en) * 2014-10-03 2020-04-16 Conagen Inc. Non-caloric sweeteners and methods for synthesizing
BR112019007504B1 (pt) 2016-10-14 2023-05-09 Conagen Inc Método de preparação de uma composição de glicosídeo de esteviol
CA3041123A1 (en) * 2016-10-20 2018-04-26 The Coca-Cola Company Diterpene glycosides isolated from stevia, compositions and methods
MX2019005570A (es) * 2016-11-14 2019-10-15 Purecircle Usa Inc Moleculas derivadas de estevia, metodos para obtener dichas moleculas y uso de las mismas.
JP7194111B2 (ja) 2017-03-06 2022-12-21 コナゲン インコーポレイテッド ステビオール配糖体レバウジオシドｄ４のレバウジオシドｅからの生合成製造
EP3644758A4 (en) * 2017-06-30 2021-03-31 Conagen Inc. Hydrolysis of steviol glycosides by beta-glucosidase
CN111868252B (zh) 2018-03-12 2025-06-03 科纳根公司 甜菊糖苷莱苞迪苷j和莱苞迪苷n的生物合成生产
MX2020009625A (es) * 2018-03-16 2021-02-16 Purecircle Usa Inc Glucosidos de esteviol de alta pureza.
JP7432533B2 (ja) * 2018-03-16 2024-02-16 ピュアサークル ユーエスエー インコーポレイテッド 高純度ステビオール配糖体
CN115867147A (zh) * 2020-05-07 2023-03-28 可口可乐公司 具有增强风味的包含莱鲍迪苷am和莱鲍迪苷m的饮料
WO2021252814A1 (en) * 2020-06-11 2021-12-16 Corn Products Development, Inc. Production and uses of oligosaccharides and steviol glycosides
WO2024068511A1 (en) * 2022-09-28 2024-04-04 Société des Produits Nestlé S.A. Diterpenoid derivatives as inhibitors of sglt2

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20160095338A1 (en) * 2014-10-03 2016-04-07 Conagen Inc. Non-caloric sweeteners and methods for synthesizing
WO2016073740A1 (en) * 2014-11-05 2016-05-12 Manus Biosynthesis, Inc. Microbial production of steviol glycosides
US20160186225A1 (en) * 2013-02-06 2016-06-30 Evolva Sa Methods for improved production of rebaudioside d and rebaudioside m
WO2016120486A1 (en) * 2015-01-30 2016-08-04 Evolva Sa Production of steviol glycosides in recombinant hosts
US20160298159A1 (en) * 2013-08-14 2016-10-13 Pepsico Inc. Enzymatic Method for Preparing Rebaudioside M

Family Cites Families (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8916138B2 (en) * 2009-10-15 2014-12-23 Purecircle Sdn Bhd High-purity rebaudioside D and low-calorie tooth paste composition containing the same
US8507022B2 (en) * 2009-10-15 2013-08-13 Purecircle Sdn Bhd High-purity rebaudioside D and low-calorie carbonated lemon-flavored beverage containing the same
JP6262653B2 (ja) 2011-08-08 2018-01-17 エヴォルヴァ エスアー．Ｅｖｏｌｖａ Ｓａ． ステビオール配糖体の組換え生産
WO2013026151A1 (en) * 2011-08-19 2013-02-28 Justbio Inc. Improved stevia rebaudiana extract and formulation, and uses thereof
US9752174B2 (en) * 2013-05-28 2017-09-05 Purecircle Sdn Bhd High-purity steviol glycosides
EP2852296B1 (en) * 2012-05-22 2021-12-15 PureCircle SDN BHD Process for producing a high-purity steviol glycoside
AU2014281353B2 (en) 2013-06-19 2017-06-29 Conagen, Inc. Rebaudioside E and food products sweetened with rebaudioside E
JP6530760B2 (ja) 2013-11-01 2019-06-12 コナゲン インコーポレーテッド ステビオール配糖体の組換え製造
US9522929B2 (en) 2014-05-05 2016-12-20 Conagen Inc. Non-caloric sweetener
EP2957182A1 (en) 2014-06-18 2015-12-23 Technische Hochschule Mittelhessen Improved natural sweetener compositions
CN113549664B (zh) * 2014-09-19 2025-10-28 谱赛科有限责任公司 高纯度甜菊醇糖苷
US10058112B2 (en) 2014-11-21 2018-08-28 Eco Sweeteners Llc Sweetener composition including enzymatically processed stevia and method of manufacturing
SG11201804542YA (en) * 2015-11-30 2018-06-28 Cargill Inc Steviol glycoside compositions for oral ingestion or use
WO2017198681A1 (en) * 2016-05-16 2017-11-23 Evolva Sa Production of steviol glycosides in recombinant hosts
SG11201811273RA (en) * 2016-06-17 2019-01-30 Cargill Inc Steviol glycoside compositions for oral ingestion or use
BR112019007504B1 (pt) 2016-10-14 2023-05-09 Conagen Inc Método de preparação de uma composição de glicosídeo de esteviol
JP7194111B2 (ja) 2017-03-06 2022-12-21 コナゲン インコーポレイテッド ステビオール配糖体レバウジオシドｄ４のレバウジオシドｅからの生合成製造
EP3644758A4 (en) 2017-06-30 2021-03-31 Conagen Inc. Hydrolysis of steviol glycosides by beta-glucosidase
US20220042060A1 (en) 2017-09-08 2022-02-10 Conagen Inc. Biosynthesis production of steviol glycosides and processes therefore

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20160186225A1 (en) * 2013-02-06 2016-06-30 Evolva Sa Methods for improved production of rebaudioside d and rebaudioside m
US20160298159A1 (en) * 2013-08-14 2016-10-13 Pepsico Inc. Enzymatic Method for Preparing Rebaudioside M
US20160095338A1 (en) * 2014-10-03 2016-04-07 Conagen Inc. Non-caloric sweeteners and methods for synthesizing
WO2016073740A1 (en) * 2014-11-05 2016-05-12 Manus Biosynthesis, Inc. Microbial production of steviol glycosides
WO2016120486A1 (en) * 2015-01-30 2016-08-04 Evolva Sa Production of steviol glycosides in recombinant hosts

Also Published As

Publication number Publication date
CN110191643B (zh) 2023-06-06
CA3039105C (en) 2024-03-05
US11680281B2 (en) 2023-06-20
KR102540615B1 (ko) 2023-06-08
JP2022153385A (ja) 2022-10-12
RU2019114181A (ru) 2020-11-16
KR20190064627A (ko) 2019-06-10
US20240229094A9 (en) 2024-07-11
JP7177048B2 (ja) 2022-11-22
US20190078102A1 (en) 2019-03-14
CN110191643A (zh) 2019-08-30
US20240132929A1 (en) 2024-04-25
ES2964976T3 (es) 2024-04-10
EP3525598C0 (en) 2023-11-29
US20210002685A1 (en) 2021-01-07
MX2019004368A (es) 2019-08-12
HUE064754T2 (hu) 2024-04-28
US11098315B2 (en) 2021-08-24
RU2019114181A3 (enExample) 2021-02-20
BR112019007504B1 (pt) 2023-05-09
WO2018071744A1 (en) 2018-04-19
AU2017341798A1 (en) 2019-04-18
JP2019535238A (ja) 2019-12-12
CA3039105A1 (en) 2018-04-19
US20190345528A1 (en) 2019-11-14
BR112019007504A2 (pt) 2019-07-02
EP3525598B1 (en) 2023-11-29
EP3525598A1 (en) 2019-08-21
EP3525598A4 (en) 2020-10-07
US10724062B2 (en) 2020-07-28

Similar Documents

Publication Publication Date Title
US20240229094A9 (en) Biosynthetic production of steviol glycosides and processes therefore
KR102378988B1 (ko) 무칼로리 감미제
JP2022082472A (ja) 高純度ステビオールグリコシド
DK3201212T3 (en) CALORIC-FREE SWEETS AND PROCEDURES FOR SYNTHETIZATION
US20220042060A1 (en) Biosynthesis production of steviol glycosides and processes therefore
US20210381019A1 (en) Biosynthetic production of variant steviol glycosides
WO2021007423A1 (en) Biosynthetic production of steviol glycoside rebaudioside i via variant enzymes
EP4403045A2 (en) Methods for making high intensity sweeteners
HK40009689A (en) Biosynthetic production of steviol glycosides and processes therefore

Legal Events

Date Code Title Description
FGA Letters patent sealed or granted (standard patent)