AU2017209041B2 - Methods and systems for rapid detection of microorganisms using infectious agents - Google Patents

Methods and systems for rapid detection of microorganisms using infectious agents Download PDF

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Publication number
AU2017209041B2
AU2017209041B2 AU2017209041A AU2017209041A AU2017209041B2 AU 2017209041 B2 AU2017209041 B2 AU 2017209041B2 AU 2017209041 A AU2017209041 A AU 2017209041A AU 2017209041 A AU2017209041 A AU 2017209041A AU 2017209041 B2 AU2017209041 B2 AU 2017209041B2
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Australia
Prior art keywords
bacteriophage
indicator
sample
hours
phage
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AU2017209041A
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AU2017209041A1 (en
Inventor
Dwight Lyman ANDERSON
Stephen Erickson
Jose S. GIL
Ben Barrett HOPKINS
Minh Mindy Bao NGUYEN
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Laboratory Corp of America Holdings
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Laboratory Corp of America Holdings
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Priority claimed from US15/263,619 external-priority patent/US20170131275A1/en
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70Vectors or expression systems specially adapted for E. coli
    • C12N15/73Expression systems using phage (lambda) regulatory sequences
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C12Q1/10Enterobacteria
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Virology (AREA)
  • General Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Toxicology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
AU2017209041A 2016-01-18 2017-01-18 Methods and systems for rapid detection of microorganisms using infectious agents Active AU2017209041B2 (en)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US201662280043P 2016-01-18 2016-01-18
US62/280,043 2016-01-18
US201662280465P 2016-01-19 2016-01-19
US62/280,465 2016-01-19
US15/263,619 2016-09-13
US15/263,619 US20170131275A1 (en) 2012-02-21 2016-09-13 Methods and systems for detection of microorganisms
PCT/US2017/013955 WO2017127434A1 (en) 2016-01-18 2017-01-18 Methods and systems for rapid detection of microorganisms using infectious agents

Publications (2)

Publication Number Publication Date
AU2017209041A1 AU2017209041A1 (en) 2018-07-26
AU2017209041B2 true AU2017209041B2 (en) 2023-03-30

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
AU2017209041A Active AU2017209041B2 (en) 2016-01-18 2017-01-18 Methods and systems for rapid detection of microorganisms using infectious agents

Country Status (8)

Country Link
EP (1) EP3405590A1 (es)
JP (2) JP2019509020A (es)
CN (2) CN108884498A (es)
AU (1) AU2017209041B2 (es)
BR (1) BR112018014166A2 (es)
CA (1) CA3011704A1 (es)
MX (1) MX2018008778A (es)
WO (1) WO2017127434A1 (es)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10519483B2 (en) 2012-02-21 2019-12-31 Laboratory Corporation Of America Holdings Methods and systems for rapid detection of microorganisms using infectious agents
EP3737746A1 (en) 2018-01-12 2020-11-18 Laboratory Corporation of America Holdings Methods and systems for the rapid detection of salmonella using infectious agents
CN112313337A (zh) * 2018-04-24 2021-02-02 美国控股实验室公司 选择和监测治疗功效的指示物噬菌体及其使用方法
BR112021013769A2 (pt) * 2019-01-29 2021-09-21 Laboratory Corporation Of America Holdings Métodos e sistemas para a detecção rápida de listeria usando agentes infecciosos.
EP3987015A1 (en) * 2019-06-21 2022-04-27 Laboratory Corporation of America Holdings Methods for producing mutant bacteriophages for the detection of listeria
CN114729332A (zh) 2019-08-26 2022-07-08 美国控股实验室公司 用于使用重组繁殖缺陷性指示噬菌体检测微生物的装置和方法
MX2022003092A (es) 2019-09-11 2022-06-08 Laboratory Corp America Holdings Metodos y sistemas para la deteccion rapida de microorganismos utilizando agentes infecciosos recombinantes para expresar una subunidad indicadora.
WO2022094393A1 (en) * 2020-10-30 2022-05-05 Albert Einstein College Of Medicine Reporter mycobacteriophage, assays and methods comprising the reporter mycobacteriophage
EP4252005A1 (en) * 2020-11-25 2023-10-04 Laboratory Corporation of America Holdings Methods and systems for the detection of microorganisms using infectious agents

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5824468A (en) * 1995-05-18 1998-10-20 Merck Patent Gesellschaft Mit Beschrankkter Haftung Detection of listeria by means of recombinant bacteriophages
WO2013126584A1 (en) * 2012-02-21 2013-08-29 Laboratory Corporation Of America Holdings Methods and systems for detection of microorganisms
WO2015126966A1 (en) * 2014-02-18 2015-08-27 Laboratory Corporation Of America Holdings Methods and systems for rapid detection of microorganisms using recombinant bacteriophage

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AU2002361548A1 (en) * 2001-07-13 2003-05-06 Investigen, Inc. Compositions and methods for bacteria detection
JP4235718B2 (ja) * 2003-03-07 2009-03-11 株式会社荏原製作所 大腸菌の検出方法及び大腸菌検出用ファージ
CN1298840C (zh) * 2003-09-24 2007-02-07 冯书章 肠出血性大肠杆菌o157基因缺失疫苗
MX2007014837A (es) * 2005-05-26 2008-02-21 Gangagen Life Sciences Inc Administracion bacterial en sistemas de retencion de animales.
EP2142667B1 (en) * 2007-04-05 2014-12-03 Sequella, Inc. Improved methods and compositions for determining the pathogenic status of infectious agents
WO2008131230A1 (en) * 2007-04-18 2008-10-30 Cornell University Microorganism detection method and apparatus
US8114622B2 (en) * 2008-08-29 2012-02-14 Purdue Research Foundation Methods for generation of reporter phages and immobilization of active bacteriophages on a polymer surface
US10519483B2 (en) * 2012-02-21 2019-12-31 Laboratory Corporation Of America Holdings Methods and systems for rapid detection of microorganisms using infectious agents
KR101299179B1 (ko) * 2012-04-18 2013-08-22 씨제이제일제당 (주) 신규 박테리오파지 및 이를 포함하는 항균 조성물
JP6300222B2 (ja) * 2013-09-25 2018-03-28 国立大学法人東京工業大学 遺伝子組換えウイルスによる微生物の迅速検出法
CA2947441A1 (en) * 2014-05-09 2015-11-12 Sample6 Technologies, Inc. Codon-optimized recombinant phage and methods of using same

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5824468A (en) * 1995-05-18 1998-10-20 Merck Patent Gesellschaft Mit Beschrankkter Haftung Detection of listeria by means of recombinant bacteriophages
WO2013126584A1 (en) * 2012-02-21 2013-08-29 Laboratory Corporation Of America Holdings Methods and systems for detection of microorganisms
WO2015126966A1 (en) * 2014-02-18 2015-08-27 Laboratory Corporation Of America Holdings Methods and systems for rapid detection of microorganisms using recombinant bacteriophage

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
ELIZABETH M KUTTER ET AL, "Characterization of a ViI-like Phage Specific to Escherichia coli O157:H7", VIROLOGY JOURNAL, BIOMED CENTRAL, LONDON, GB, (2011-09-07), vol. 8, no. 1, doi:10.1186/1743-422X-8-430, ISSN 1743-422X, page 430 *
Tanji et al. Escherichia coli detection by GFP-labeled lysozyme-inactivated T4 bacteriophage. Journal of Biotechnology 114 (2004) 11-20. *

Also Published As

Publication number Publication date
WO2017127434A1 (en) 2017-07-27
MX2018008778A (es) 2018-09-12
AU2017209041A1 (en) 2018-07-26
BR112018014166A2 (pt) 2018-12-26
JP2022024167A (ja) 2022-02-08
JP2019509020A (ja) 2019-04-04
CN108884498A (zh) 2018-11-23
CN115960844A (zh) 2023-04-14
EP3405590A1 (en) 2018-11-28
CA3011704A1 (en) 2017-07-27

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