AU2016357567B2 - Media and fermentation methods for producing polysaccharides in bacterial cell culture - Google Patents

Media and fermentation methods for producing polysaccharides in bacterial cell culture Download PDF

Info

Publication number
AU2016357567B2
AU2016357567B2 AU2016357567A AU2016357567A AU2016357567B2 AU 2016357567 B2 AU2016357567 B2 AU 2016357567B2 AU 2016357567 A AU2016357567 A AU 2016357567A AU 2016357567 A AU2016357567 A AU 2016357567A AU 2016357567 B2 AU2016357567 B2 AU 2016357567B2
Authority
AU
Australia
Prior art keywords
medium
polysaccharide
concentration
vvh
perfusion
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
AU2016357567A
Other languages
English (en)
Other versions
AU2016357567A1 (en
Inventor
Sunil Gururao DESAI
Michael Allen Hanson
Jonathan Patrick KINROSS
Daniel R. Lasko
Scott Ellis LOMBERK
Jason Arnold Lotvin
Sujata Kaushikbhai PATEL-BROWN
Weiqiang SUN
Peter Anthony TOMASELLO
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Pfizer Inc
Original Assignee
Pfizer Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pfizer Inc filed Critical Pfizer Inc
Publication of AU2016357567A1 publication Critical patent/AU2016357567A1/en
Application granted granted Critical
Publication of AU2016357567B2 publication Critical patent/AU2016357567B2/en
Ceased legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/46—Streptococcus ; Enterococcus; Lactococcus
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
AU2016357567A 2015-11-17 2016-11-10 Media and fermentation methods for producing polysaccharides in bacterial cell culture Ceased AU2016357567B2 (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201562256347P 2015-11-17 2015-11-17
US62/256,347 2015-11-17
US201662413051P 2016-10-26 2016-10-26
US62/413,051 2016-10-26
PCT/IB2016/056780 WO2017085602A1 (en) 2015-11-17 2016-11-10 Media and fermentation methods for producing polysaccharides in bacterial cell culture

Publications (2)

Publication Number Publication Date
AU2016357567A1 AU2016357567A1 (en) 2018-05-17
AU2016357567B2 true AU2016357567B2 (en) 2020-05-07

Family

ID=57394621

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2016357567A Ceased AU2016357567B2 (en) 2015-11-17 2016-11-10 Media and fermentation methods for producing polysaccharides in bacterial cell culture

Country Status (13)

Country Link
US (3) US10947494B2 (enExample)
EP (1) EP3377613B1 (enExample)
JP (3) JP7311966B2 (enExample)
KR (1) KR102069988B1 (enExample)
CN (1) CN108473945A (enExample)
AU (1) AU2016357567B2 (enExample)
BR (1) BR112018008864A8 (enExample)
CA (2) CA3138763A1 (enExample)
HK (1) HK1259103A1 (enExample)
IL (2) IL259404B2 (enExample)
MX (2) MX385736B (enExample)
RU (2) RU2021135165A (enExample)
WO (1) WO2017085602A1 (enExample)

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AR086199A1 (es) 2011-04-22 2013-11-27 Wyeth Llc Composiciones relacionadas con una toxina de clostridium difficile mutante y sus metodos
SI2726600T1 (sl) 2011-07-01 2017-07-31 Amgen Inc. Sesalska celična kultura
BR122016023101B1 (pt) 2012-10-21 2022-03-22 Pfizer Inc Polipeptídeo, composição imunogênica que o compreende, bem como célula recombinante derivada de clostridium difficile
CN109890413B (zh) 2016-09-02 2024-04-16 赛诺菲巴斯德股份有限公司 脑膜炎奈瑟氏菌疫苗
WO2018144439A1 (en) 2017-01-31 2018-08-09 Merck Sharp & Dohme Corp. Methods for making polysaccharide-protein conjugates
JP7680185B2 (ja) 2017-02-24 2025-05-20 メルク・シャープ・アンド・ドーム・エルエルシー 肺炎連鎖球菌多糖－タンパク質コンジュゲートの免疫原性の増強
CN111065387B (zh) 2017-09-07 2023-08-25 默沙东有限责任公司 肺炎球菌多糖及其在免疫原性多糖-载体蛋白缀合物中的用途
US11395849B2 (en) 2017-09-07 2022-07-26 Merck Sharp & Dohme Llc Pneumococcal polysaccharides and their use in immunogenic polysaccharide-carrier protein conjugates
EP3720483B1 (en) 2017-12-06 2025-11-19 Merck Sharp & Dohme LLC Compositions comprising streptococcus pneumoniae polysaccharide-protein conjugates and methods of use thereof
WO2019212842A1 (en) 2018-04-30 2019-11-07 Merck Sharp & Dohme Corp. Methods for producing streptococcus pneumoniae capsular polysaccharide carrier protein conjugates from lyospheres
WO2019212846A1 (en) 2018-04-30 2019-11-07 Merck Sharp & Dohme Corp. Methods for producing streptococcus pneumoniae capsular polysaccharide carrier protein conjugates
JP7397000B2 (ja) 2018-04-30 2023-12-12 メルク・シャープ・アンド・ドーム・エルエルシー 凍結乾燥変異ジフテリア毒素のジメチルスルホキシド中均一溶液を提供する方法
KR102609289B1 (ko) * 2018-12-06 2023-12-01 인벤트프라이즈 인크. 비-동물성 배지에서의 박테리아 증식
WO2020123524A1 (en) * 2018-12-11 2020-06-18 Erbi Biosystems, Inc. Methods of manufacturing cell based products using small volume perfusion processes
EP3897705A2 (en) 2018-12-19 2021-10-27 Merck Sharp & Dohme Corp. Compositions comprising streptococcus pneumoniae polysaccharide-protein conjugates and methods of use thereof
CN111254103B (zh) * 2019-11-19 2023-06-02 河南省生物工程技术研究中心 一种非洲猪瘟基因工程疫苗高密度发酵补料培养基及发酵工艺
JP2023522059A (ja) * 2020-04-20 2023-05-26 エボニック オペレーションズ ゲーエムベーハー Ｃｙｓ－ペプチドを含む組成物
WO2021255690A2 (en) 2020-06-19 2021-12-23 Pfizer Inc. Immunogenic compositions against clostridioides (clostridium) difficile and methods thereof
CN114015622A (zh) * 2021-12-14 2022-02-08 塔里木大学 无乳链球菌培养基及其制备方法
WO2023131588A1 (en) * 2022-01-05 2023-07-13 Nordmark Pharma Gmbh Culture medium for cultivating hathewaya histolytica (or clostridium histolyticum) and the production of one or more proteases
CN114181872B (zh) * 2022-01-07 2023-11-24 北京大北农科技集团股份有限公司 一种屎肠球菌高密度发酵培养方法
KR102864280B1 (ko) * 2022-12-27 2025-09-26 (주)진성유니텍 포도상구균 증균을 위한 최소배지 및 이를 이용한 포도상구균 증균방법
KR102866798B1 (ko) * 2022-12-27 2025-10-01 (주)진성유니텍 살모넬라균 증균을 위한 최소배지 및 이를 이용한 살모넬라균 증균방법
CN117821342B (zh) * 2024-02-29 2024-06-04 北京民海生物科技有限公司 一种14型肺炎链球菌的发酵方法
KR102894728B1 (ko) 2024-11-27 2025-12-08 한국콜마주식회사 피지 생성 억제 효능, 모공 개선 효능 및 피부 장벽 강화 효능이 우수한 흑효모 발효물 및 이의 제조방법

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998054296A1 (en) * 1997-05-28 1998-12-03 Chiron S.P.A. Culture medium with yeast or soy bean extract as aminoacid source and no protein complexes of animal origin
WO2003012058A2 (en) * 2001-08-02 2003-02-13 North Carolina State University Media and methods for cultivation of microorganisms
WO2011151841A1 (en) * 2010-05-31 2011-12-08 Panacea Biotec Limited Fermentation process for streptococcus pneumoniae

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5801054A (en) * 1996-09-19 1998-09-01 The United States Of America As Represented By The Secretary Of The Air Force Cell culture vessel with self-maintained atmosphere
US7294484B2 (en) 2004-08-27 2007-11-13 Wyeth Research Ireland Limited Production of polypeptides
US7472101B2 (en) 2005-07-12 2008-12-30 Tibco Software Inc. Inferential state machines
GB0522303D0 (en) 2005-11-01 2005-12-07 Chiron Srl Culture method
US7491517B2 (en) * 2006-07-19 2009-02-17 Jeeri R Reddy Method of producing meningococcal meningitis vaccine for Neisseria meningitidis serotypes A,C,Y, and W-135
MX2009004974A (es) 2006-11-08 2009-05-20 Wyeth Corp Medios diseñado racionalmente para cultivo celular.
GB0818453D0 (en) * 2008-10-08 2008-11-12 Novartis Ag Fermentation processes for cultivating streptococci and purification processes for obtaining cps therefrom
WO2012155295A1 (zh) * 2011-05-17 2012-11-22 中国食品发酵工业研究院 一种工业化生产玉米降压活性肽的方法
IN2014MN02530A (enExample) * 2011-12-15 2015-07-24 Serum Inst India Ltd
EP2647715A1 (en) * 2012-04-03 2013-10-09 Nomad Bioscience GmbH Agrobacterium for transient transfection of whole plants
GB201211256D0 (en) * 2012-06-25 2012-08-08 Glaxosmithkline Biolog Sa Fermentation process
NZ708523A (en) * 2012-11-21 2018-09-28 Serum Institute Of India Pvt Ltd Production of high yields of bacterial polysaccharides

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998054296A1 (en) * 1997-05-28 1998-12-03 Chiron S.P.A. Culture medium with yeast or soy bean extract as aminoacid source and no protein complexes of animal origin
WO2003012058A2 (en) * 2001-08-02 2003-02-13 North Carolina State University Media and methods for cultivation of microorganisms
WO2011151841A1 (en) * 2010-05-31 2011-12-08 Panacea Biotec Limited Fermentation process for streptococcus pneumoniae

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
MICOLI F ET AL, "Development of a glycoconjugate vaccine to prevent meningitis in Africa caused by meningococcal serogroup X", PNAS, 2013, vol 110(47), 19077-82 *
SMITH LD ET AL, "Growth of Pasteurella pestis on a casein digest medium", JOURNAL OF THE FRANKLIN INSTITUTE, 1943, vol 235, 536-545 *
Thermofisher Scientific - Technical Resources - 11595, Grace's Insect Medium, Unsupplemented [retrieved from internet on 28 January 2020] <URL: https://www.thermofisher.com/au/en/home/technical-resources/media-formulation.71.html> *
VITORIO, MB ET AL, "Capsular polysaccharide production byStreptococcus pneumoniaeserotype 1: from strain selection to fed-batch cultivation", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, vol 99, no 24, doi:10.1007/S00253-015-6928-Z (2015-08-23) *

Also Published As

Publication number Publication date
US10947494B2 (en) 2021-03-16
HK1259103A1 (zh) 2019-11-22
RU2021135165A (ru) 2021-12-29
MX385736B (es) 2025-03-18
KR20180073699A (ko) 2018-07-02
IL259404B1 (en) 2025-04-01
US20190292513A1 (en) 2019-09-26
JP2018533366A (ja) 2018-11-15
JP7311966B2 (ja) 2023-07-20
CA3005308A1 (en) 2017-05-26
MX2018006063A (es) 2018-09-17
JP7814142B2 (ja) 2026-02-16
JP2024037949A (ja) 2024-03-19
RU2018118035A (ru) 2019-12-18
CA3138763A1 (en) 2017-05-26
CN108473945A (zh) 2018-08-31
RU2761633C2 (ru) 2021-12-13
EP3377613A1 (en) 2018-09-26
IL319202A (en) 2025-04-01
US20210180008A1 (en) 2021-06-17
BR112018008864A2 (pt) 2018-11-06
IL259404B2 (en) 2025-08-01
WO2017085602A1 (en) 2017-05-26
KR102069988B1 (ko) 2020-01-23
IL259404A (en) 2018-07-31
EP3377613B1 (en) 2026-07-15
RU2018118035A3 (enExample) 2019-12-18
JP2022017529A (ja) 2022-01-25
MX2021010518A (es) 2021-10-01
CA3005308C (en) 2022-01-04
AU2016357567A1 (en) 2018-05-17
BR112018008864A8 (pt) 2019-02-26
US20260008994A1 (en) 2026-01-08

Similar Documents

Publication Publication Date Title
JP7814142B2 (ja) 細菌細胞培養物中で多糖を生成するための培地および発酵方法
EP2922569B1 (en) Production of high yields of bacterial polysaccharides
US10144940B2 (en) Method for producing Haemophilus influenzae type B antigens
US20080274515A1 (en) Animal component free meningococcal polysaccharide fermentation and seedbank development
WO2011151841A1 (en) Fermentation process for streptococcus pneumoniae
WO2025219908A2 (en) Media and fermentation methods for polysaccharide production in bacterial cell culture

Legal Events

Date Code Title Description
FGA Letters patent sealed or granted (standard patent)
MK14 Patent ceased section 143(a) (annual fees not paid) or expired