AU2016335698B2 - Multiplexed genome editing - Google Patents

Multiplexed genome editing Download PDF

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Publication number
AU2016335698B2
AU2016335698B2 AU2016335698A AU2016335698A AU2016335698B2 AU 2016335698 B2 AU2016335698 B2 AU 2016335698B2 AU 2016335698 A AU2016335698 A AU 2016335698A AU 2016335698 A AU2016335698 A AU 2016335698A AU 2016335698 B2 AU2016335698 B2 AU 2016335698B2
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cell
perv
porcine
genetically engineered
sequence
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AU2016335698A1 (en
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George M. Church
Marc Guell
Luhan Yang
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Harvard University
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Harvard University
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    • A—HUMAN NECESSITIES
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    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0275—Genetically modified vertebrates, e.g. transgenic
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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    • A01K67/0273—Cloned vertebrates
    • A—HUMAN NECESSITIES
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    • A01K67/027—New or modified breeds of vertebrates
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    • A01K67/0276—Knock-out vertebrates
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    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/02—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with ribosyl as saccharide radical
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    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
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    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
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    • C12N15/09—Recombinant DNA-technology
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    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C12N15/1131—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against viruses
    • C12N15/1132—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against viruses against retroviridae, e.g. HIV
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
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    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
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    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
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    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C12N9/222—Clustered regularly interspaced short palindromic repeats [CRISPR]-associated [CAS] enzymes
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    • C12N2310/00—Structure or type of the nucleic acid
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    • C12N2740/00011—Details
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AU2016335698A 2015-10-08 2016-10-07 Multiplexed genome editing Active AU2016335698B2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2023201178A AU2023201178B2 (en) 2015-10-08 2023-02-28 Multiplexed genome editing

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201562239239P 2015-10-08 2015-10-08
US62/239,239 2015-10-08
PCT/US2016/055916 WO2017062723A1 (en) 2015-10-08 2016-10-07 Multiplexed genome editing

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AU2016335698A1 AU2016335698A1 (en) 2018-05-10
AU2016335698B2 true AU2016335698B2 (en) 2022-12-01

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US (6) US10959413B2 (https=)
EP (2) EP3359661B1 (https=)
JP (4) JP7059468B2 (https=)
KR (2) KR102729669B1 (https=)
CN (2) CN108513580A (https=)
AU (2) AU2016335698B2 (https=)
CA (1) CA3001314A1 (https=)
CL (1) CL2018000902A1 (https=)
CO (1) CO2018004763A2 (https=)
IL (2) IL297017A (https=)
MX (2) MX2018004263A (https=)
NZ (1) NZ741778A (https=)
SA (2) SA523441205B1 (https=)
WO (1) WO2017062723A1 (https=)
ZA (1) ZA201802461B (https=)

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KR20240116572A (ko) 2017-03-23 2024-07-29 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 핵산 프로그램가능한 dna 결합 단백질을 포함하는 핵염기 편집제
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