AU2016258980B2 - Potency assay - Google Patents

Potency assay Download PDF

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Publication number
AU2016258980B2
AU2016258980B2 AU2016258980A AU2016258980A AU2016258980B2 AU 2016258980 B2 AU2016258980 B2 AU 2016258980B2 AU 2016258980 A AU2016258980 A AU 2016258980A AU 2016258980 A AU2016258980 A AU 2016258980A AU 2016258980 B2 AU2016258980 B2 AU 2016258980B2
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AU
Australia
Prior art keywords
cells
mpc
culture medium
mpcs
human
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AU2016258980A
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AU2016258980A1 (en
Inventor
Fiona SEE
Paul Simmons
Colby SUIRE
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Mesoblast International SARL
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Mesoblast International SARL
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Priority claimed from AU2015901605A external-priority patent/AU2015901605A0/en
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Publication of AU2016258980B2 publication Critical patent/AU2016258980B2/en
Priority to AU2022203140A priority Critical patent/AU2022203140B2/en
Priority to AU2025205043A priority patent/AU2025205043A1/en
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
    • G01N33/5073—Stem cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/28—Bone marrow; Haematopoietic stem cells; Mesenchymal stem cells of any origin, e.g. adipose-derived stem cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/30—Macromolecular organic or inorganic compounds, e.g. inorganic polyphosphates
    • A61K47/36—Polysaccharides; Derivatives thereof, e.g. gums, starch, alginate, dextrin, hyaluronic acid, chitosan, inulin, agar or pectin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P19/00—Drugs for skeletal disorders
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P19/00—Drugs for skeletal disorders
    • A61P19/04—Drugs for skeletal disorders for non-specific disorders of the connective tissue
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P19/00—Drugs for skeletal disorders
    • A61P19/08—Drugs for skeletal disorders for bone diseases, e.g. rachitism, Paget's disease
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C12N5/0663—Bone marrow mesenchymal stem cells (BM-MSC)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6863—Cytokines, i.e. immune system proteins modifying a biological response such as cell growth proliferation or differentiation, e.g. TNF, CNF, GM-CSF, lymphotoxin, MIF or their receptors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/15—Transforming growth factor beta (TGF-β)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/475—Assays involving growth factors
    • G01N2333/495—Transforming growth factor [TGF]
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2800/00—Detection or diagnosis of diseases
    • G01N2800/10—Musculoskeletal or connective tissue disorders

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Cell Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Urology & Nephrology (AREA)
  • Veterinary Medicine (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Epidemiology (AREA)
  • Physics & Mathematics (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Rheumatology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Physical Education & Sports Medicine (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Virology (AREA)
  • General Engineering & Computer Science (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Toxicology (AREA)
AU2016258980A 2015-05-05 2016-05-04 Potency assay Active AU2016258980B2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
AU2022203140A AU2022203140B2 (en) 2015-05-05 2022-05-11 Potency assay
AU2025205043A AU2025205043A1 (en) 2015-05-05 2025-07-02 Potency assay

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
AU2015901605 2015-05-05
AU2015901605A AU2015901605A0 (en) 2015-05-05 Potency assay
PCT/EP2016/060049 WO2016177805A1 (en) 2015-05-05 2016-05-04 Potency assay

Related Child Applications (1)

Application Number Title Priority Date Filing Date
AU2022203140A Division AU2022203140B2 (en) 2015-05-05 2022-05-11 Potency assay

Publications (2)

Publication Number Publication Date
AU2016258980A1 AU2016258980A1 (en) 2017-11-23
AU2016258980B2 true AU2016258980B2 (en) 2022-02-17

Family

ID=56087237

Family Applications (3)

Application Number Title Priority Date Filing Date
AU2016258980A Active AU2016258980B2 (en) 2015-05-05 2016-05-04 Potency assay
AU2022203140A Active AU2022203140B2 (en) 2015-05-05 2022-05-11 Potency assay
AU2025205043A Pending AU2025205043A1 (en) 2015-05-05 2025-07-02 Potency assay

Family Applications After (2)

Application Number Title Priority Date Filing Date
AU2022203140A Active AU2022203140B2 (en) 2015-05-05 2022-05-11 Potency assay
AU2025205043A Pending AU2025205043A1 (en) 2015-05-05 2025-07-02 Potency assay

Country Status (9)

Country Link
US (3) US11795435B2 (enExample)
EP (2) EP4015624B1 (enExample)
JP (3) JP6797833B2 (enExample)
KR (2) KR102582602B1 (enExample)
CN (2) CN107771220B (enExample)
AU (3) AU2016258980B2 (enExample)
CA (1) CA2984987C (enExample)
ES (2) ES2911266T3 (enExample)
WO (1) WO2016177805A1 (enExample)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
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CN112004923A (zh) 2017-11-22 2020-11-27 迈索布拉斯特国际有限公司 细胞组合物及治疗方法
KR102506822B1 (ko) * 2018-09-20 2023-03-06 시토스핀, 에스.엘. 기능적 중간엽 줄기 세포의 농축된 집단을 수득하는 방법, 이의 수득된 세포 및 이를 포함하는 조성물
WO2020141473A1 (en) * 2019-01-02 2020-07-09 Mesoblast International Sárl Method for treating lower back pain
US20230221303A1 (en) * 2020-04-20 2023-07-13 Longeveron Inc. Potency assay
EP4641205A1 (en) * 2022-12-23 2025-10-29 Kangstem Biotech Co., Ltd. Method for selecting stem cells with enhanced cartilage differentiation capability and cell therapy product comprising same

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Also Published As

Publication number Publication date
CA2984987C (en) 2023-10-10
KR102582602B1 (ko) 2023-09-25
AU2016258980A1 (en) 2017-11-23
JP7754893B2 (ja) 2025-10-15
EP3292199B1 (en) 2022-01-12
JP7335222B2 (ja) 2023-08-29
JP6797833B2 (ja) 2020-12-09
US20240409898A1 (en) 2024-12-12
BR112017023660A2 (pt) 2018-07-17
US20240076621A1 (en) 2024-03-07
KR20230140604A (ko) 2023-10-06
CA2984987A1 (en) 2016-11-10
WO2016177805A1 (en) 2016-11-10
JP2018517900A (ja) 2018-07-05
AU2022203140A1 (en) 2022-06-02
EP4015624B1 (en) 2025-02-19
JP2021036892A (ja) 2021-03-11
US20200140822A1 (en) 2020-05-07
EP4015624A1 (en) 2022-06-22
CN107771220B (zh) 2022-07-05
CN107771220A (zh) 2018-03-06
JP2023159260A (ja) 2023-10-31
HK1247236A1 (zh) 2018-09-21
AU2025205043A1 (en) 2025-07-24
EP3292199A1 (en) 2018-03-14
KR102885856B1 (ko) 2025-11-14
CN115141872A (zh) 2022-10-04
KR20180041621A (ko) 2018-04-24
ES2911266T3 (es) 2022-05-18
US11795435B2 (en) 2023-10-24
ES3018944T3 (en) 2025-05-19
AU2022203140B2 (en) 2025-04-10

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