AU2016231995B2 - PCR method - Google Patents

PCR method Download PDF

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Publication number
AU2016231995B2
AU2016231995B2 AU2016231995A AU2016231995A AU2016231995B2 AU 2016231995 B2 AU2016231995 B2 AU 2016231995B2 AU 2016231995 A AU2016231995 A AU 2016231995A AU 2016231995 A AU2016231995 A AU 2016231995A AU 2016231995 B2 AU2016231995 B2 AU 2016231995B2
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AU
Australia
Prior art keywords
sequence
oligonucleotide probe
target
universal
sequences
Prior art date
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Active
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AU2016231995A
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English (en)
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AU2016231995A1 (en
Inventor
Christopher John MATTOCKS
Daniel Leonard WARD
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Salisbury NHS Foundation Trust
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Salisbury NHS Foundation Trust
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Publication date
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Publication of AU2016231995A1 publication Critical patent/AU2016231995A1/en
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/161—Modifications characterised by incorporating target specific and non-target specific sites
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/186—Modifications characterised by incorporating a non-extendable or blocking moiety
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
    • C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
    • C12Q2537/101—Homogeneous assay format, e.g. one pot reaction

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AU2016231995A 2015-03-17 2016-03-03 PCR method Active AU2016231995B2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB1504464.7A GB2536446B (en) 2015-03-17 2015-03-17 PCR method
GB1504464.7 2015-03-17
PCT/GB2016/050558 WO2016146968A1 (en) 2015-03-17 2016-03-03 Pcr method

Publications (2)

Publication Number Publication Date
AU2016231995A1 AU2016231995A1 (en) 2017-10-12
AU2016231995B2 true AU2016231995B2 (en) 2021-07-15

Family

ID=53016243

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2016231995A Active AU2016231995B2 (en) 2015-03-17 2016-03-03 PCR method

Country Status (10)

Country Link
US (1) US11499182B2 (ja)
EP (1) EP3271475A1 (ja)
JP (1) JP6940412B2 (ja)
CN (1) CN107406891B (ja)
AU (1) AU2016231995B2 (ja)
CA (1) CA3017443A1 (ja)
GB (1) GB2536446B (ja)
HK (1) HK1244036A1 (ja)
NZ (1) NZ735738A (ja)
WO (1) WO2016146968A1 (ja)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108486101B (zh) * 2018-04-03 2021-01-08 艾吉泰康生物科技(北京)有限公司 一种快速捕获建库的方法
CN108774639B (zh) * 2018-05-31 2023-05-30 澳門帝傑數碼基因有限公司 一种定向聚合的荧光探针pcr
US20220411861A1 (en) 2019-09-11 2022-12-29 Parlanca Limited A Multiplex Method of Preparing a Sequencing Library

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007062495A1 (en) * 2005-11-30 2007-06-07 Roy Rabindranauth Sooknanan Selective terminal tagging of nucleic acids
WO2010057525A1 (en) * 2008-11-19 2010-05-27 Fondazione Parco Tecnologico Padano Oligonucleotide primers for nucleotide indexing of polymorphic pcr products and methods for their use
WO2012054933A2 (en) * 2010-10-22 2012-04-26 Fluidigm Corporation Universal probe assay methods

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Publication number Priority date Publication date Assignee Title
US6207242B1 (en) * 1995-12-28 2001-03-27 Hoffman Environmental System, Inc. Laminated package with enhanced interior and exterior
WO2001006012A1 (en) * 1999-07-14 2001-01-25 Packard Bioscience Company Derivative nucleic acids and uses thereof
GB0013260D0 (en) * 2000-05-31 2000-07-19 Neuronova Ab Method
GB0019179D0 (en) * 2000-08-07 2000-09-27 Potter Colin G Genetic analysis with a universal probe system
AUPS204502A0 (en) * 2002-05-01 2002-06-06 Nucleics Pty Ltd A method for increasing the affinity of an oligonucleotide for a target nucleic acid
US7176002B2 (en) * 2002-05-16 2007-02-13 Applera Corporation Universal-tagged oligonucleotide primers and methods of use
EP1602733A1 (en) * 2004-06-02 2005-12-07 Keygene N.V. Detection of target nucleotide sequences using an asymmetric oligonucleotide ligation assay
US9657347B2 (en) * 2004-04-20 2017-05-23 University of Utah Research Foundation and BioFire Defense, LLC Nucleic acid melting analysis with saturation dyes
EP1991698B1 (en) * 2006-03-01 2013-12-18 Keygene N.V. High throughput sequence-based detection of snps using ligation assays
WO2007130967A2 (en) * 2006-05-01 2007-11-15 Siemens Healthcare Diagnostics Inc. Novel oligonucleotide primers and methods for dna replication
US8486638B2 (en) * 2008-10-29 2013-07-16 Check-Points Holding B.V. Method for fast detection and identification of micro-organisms
US9017971B2 (en) * 2008-11-05 2015-04-28 Stichting Sanquin Bloedvoorziening Means and methods for investigating nucleic acid sequences
CN103491871B (zh) * 2011-04-22 2016-04-20 皇家飞利浦有限公司 患者定位系统
EP2729580B1 (en) * 2011-07-08 2015-09-16 Keygene N.V. Sequence based genotyping based on oligonucleotide ligation assays
US8987174B2 (en) * 2011-10-28 2015-03-24 Prognosys Biosciences, Inc. Methods for manufacturing molecular arrays
ES2662825T3 (es) 2013-02-25 2018-04-09 Seegene, Inc. Detección de variación de nucleótido en una secuencia de ácidos nucleicos diana
US11254977B2 (en) * 2013-03-12 2022-02-22 Life Technologies Corporation Universal reporter-based genotyping methods and materials

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007062495A1 (en) * 2005-11-30 2007-06-07 Roy Rabindranauth Sooknanan Selective terminal tagging of nucleic acids
WO2010057525A1 (en) * 2008-11-19 2010-05-27 Fondazione Parco Tecnologico Padano Oligonucleotide primers for nucleotide indexing of polymorphic pcr products and methods for their use
WO2012054933A2 (en) * 2010-10-22 2012-04-26 Fluidigm Corporation Universal probe assay methods

Also Published As

Publication number Publication date
GB2536446A (en) 2016-09-21
AU2016231995A1 (en) 2017-10-12
JP6940412B2 (ja) 2021-09-29
CN107406891A (zh) 2017-11-28
CN107406891B (zh) 2022-06-24
US20180073053A1 (en) 2018-03-15
CA3017443A1 (en) 2016-09-22
NZ735738A (en) 2024-01-26
HK1244036A1 (zh) 2018-07-27
GB201504464D0 (en) 2015-04-29
US11499182B2 (en) 2022-11-15
WO2016146968A1 (en) 2016-09-22
JP2018507706A (ja) 2018-03-22
GB2536446B (en) 2020-06-03
EP3271475A1 (en) 2018-01-24

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