HK1244036A1 - Pcr方法 - Google Patents

Pcr方法

Info

Publication number
HK1244036A1
HK1244036A1 HK18103563.1A HK18103563A HK1244036A1 HK 1244036 A1 HK1244036 A1 HK 1244036A1 HK 18103563 A HK18103563 A HK 18103563A HK 1244036 A1 HK1244036 A1 HK 1244036A1
Authority
HK
Hong Kong
Prior art keywords
pcr method
pcr
Prior art date
Application number
HK18103563.1A
Other languages
English (en)
Inventor
D‧L‧沃德
C‧J‧馬托克斯
Original Assignee
索爾茲伯裡Nhs信托基金會
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 索爾茲伯裡Nhs信托基金會 filed Critical 索爾茲伯裡Nhs信托基金會
Publication of HK1244036A1 publication Critical patent/HK1244036A1/zh

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6853Nucleic acid amplification reactions using modified primers or templates
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/686Polymerase chain reaction [PCR]
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10Modifications characterised by
    • C12Q2525/161Modifications characterised by incorporating target specific and non-target specific sites
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10Modifications characterised by
    • C12Q2525/186Modifications characterised by incorporating a non-extendable or blocking moiety
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2537/00Reactions characterised by the reaction format or use of a specific feature
    • C12Q2537/10Reactions characterised by the reaction format or use of a specific feature the purpose or use of
    • C12Q2537/101Homogeneous assay format, e.g. one pot reaction

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
HK18103563.1A 2015-03-17 2018-03-14 Pcr方法 HK1244036A1 (zh)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB1504464.7A GB2536446B (en) 2015-03-17 2015-03-17 PCR method
PCT/GB2016/050558 WO2016146968A1 (en) 2015-03-17 2016-03-03 Pcr method

Publications (1)

Publication Number Publication Date
HK1244036A1 true HK1244036A1 (zh) 2018-07-27

Family

ID=53016243

Family Applications (1)

Application Number Title Priority Date Filing Date
HK18103563.1A HK1244036A1 (zh) 2015-03-17 2018-03-14 Pcr方法

Country Status (9)

Country Link
US (1) US11499182B2 (zh)
EP (1) EP3271475A1 (zh)
JP (1) JP6940412B2 (zh)
CN (1) CN107406891B (zh)
AU (1) AU2016231995B2 (zh)
CA (1) CA3017443A1 (zh)
GB (1) GB2536446B (zh)
HK (1) HK1244036A1 (zh)
WO (1) WO2016146968A1 (zh)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108486101B (zh) * 2018-04-03 2021-01-08 艾吉泰康生物科技(北京)有限公司 一种快速捕获建库的方法
CN108774639B (zh) * 2018-05-31 2023-05-30 澳門帝傑數碼基因有限公司 一种定向聚合的荧光探针pcr
JP2022548000A (ja) 2019-09-11 2022-11-16 パランカ・リミテッド シーケンシングライブラリーの多重製造方法

Family Cites Families (20)

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US6207242B1 (en) * 1995-12-28 2001-03-27 Hoffman Environmental System, Inc. Laminated package with enhanced interior and exterior
CA2379132C (en) * 1999-07-14 2011-05-31 Packard Bioscience Company Method for multiplexed analysis of a plurality of target nucleic acid sequences in a sample
GB0013260D0 (en) * 2000-05-31 2000-07-19 Neuronova Ab Method
GB0019179D0 (en) * 2000-08-07 2000-09-27 Potter Colin G Genetic analysis with a universal probe system
AUPS204502A0 (en) * 2002-05-01 2002-06-06 Nucleics Pty Ltd A method for increasing the affinity of an oligonucleotide for a target nucleic acid
US7176002B2 (en) * 2002-05-16 2007-02-13 Applera Corporation Universal-tagged oligonucleotide primers and methods of use
EP1602733A1 (en) * 2004-06-02 2005-12-07 Keygene N.V. Detection of target nucleotide sequences using an asymmetric oligonucleotide ligation assay
US9657347B2 (en) * 2004-04-20 2017-05-23 University of Utah Research Foundation and BioFire Defense, LLC Nucleic acid melting analysis with saturation dyes
CA2639819C (en) * 2005-11-30 2012-10-23 Epicentre Technologies Corporation Selective terminal tagging of nucleic acids
JP5237126B2 (ja) * 2006-03-01 2013-07-17 キージーン ナムローゼ フェンノートシャップ ライゲーションアッセイを用いてハイスループットシークエンスに基づき遺伝子関連配列を検出する方法
EP2013366B1 (en) * 2006-05-01 2016-11-30 Siemens Healthcare Diagnostics Inc. Sequencing of the L10 codon of the HIV gag gene
ES2399318T3 (es) * 2008-10-29 2013-03-27 Check-Points Holding B.V. Método para la detección e identificación rápidas de microorganismos
EP2356253A1 (en) * 2008-11-05 2011-08-17 Stichting Sanquin Bloedvoorziening Means and methods for investigating nucleic acid sequences
WO2010057525A1 (en) * 2008-11-19 2010-05-27 Fondazione Parco Tecnologico Padano Oligonucleotide primers for nucleotide indexing of polymorphic pcr products and methods for their use
US9353406B2 (en) * 2010-10-22 2016-05-31 Fluidigm Corporation Universal probe assay methods
US9901310B2 (en) * 2011-04-22 2018-02-27 Koninklijke Philips N.V. Patient localization system
DK2729580T3 (en) * 2011-07-08 2015-12-14 Keygene Nv SEQUENCE BASED genotyping BASED ON OLIGONUKLEOTIDLIGERINGSASSAYS
US8987174B2 (en) * 2011-10-28 2015-03-24 Prognosys Biosciences, Inc. Methods for manufacturing molecular arrays
EP2770065B1 (en) * 2013-02-25 2017-12-13 Seegene, Inc. Detection of nucleotide variation on target nucleic acid sequence
EP2971095B1 (en) * 2013-03-12 2019-11-20 Life Technologies Corporation Universal reporter-based genotyping methods, reaction mixture and kit

Also Published As

Publication number Publication date
US11499182B2 (en) 2022-11-15
JP2018507706A (ja) 2018-03-22
WO2016146968A1 (en) 2016-09-22
EP3271475A1 (en) 2018-01-24
JP6940412B2 (ja) 2021-09-29
NZ735738A (en) 2024-01-26
CN107406891B (zh) 2022-06-24
CN107406891A (zh) 2017-11-28
GB2536446B (en) 2020-06-03
GB201504464D0 (en) 2015-04-29
US20180073053A1 (en) 2018-03-15
GB2536446A (en) 2016-09-21
AU2016231995B2 (en) 2021-07-15
AU2016231995A1 (en) 2017-10-12
CA3017443A1 (en) 2016-09-22

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