AU2014278537B2 - Methods, kits, and systems for multiplexed detection of target molecules and uses thereof - Google Patents

Methods, kits, and systems for multiplexed detection of target molecules and uses thereof Download PDF

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AU2014278537B2
AU2014278537B2 AU2014278537A AU2014278537A AU2014278537B2 AU 2014278537 B2 AU2014278537 B2 AU 2014278537B2 AU 2014278537 A AU2014278537 A AU 2014278537A AU 2014278537 A AU2014278537 A AU 2014278537A AU 2014278537 B2 AU2014278537 B2 AU 2014278537B2
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pct
cells
probes
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AU2014278537A1 (en
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Sarit S. AGASTI
Vanessa M. PETERSON
Adeeti ULLAL
Ralph Weissleder
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General Hospital Corp
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6804Nucleic acid analysis using immunogens
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6816Hybridisation assays characterised by the detection means
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6816Hybridisation assays characterised by the detection means
    • C12Q1/6823Release of bound markers
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2458/00Labels used in chemical analysis of biological material
    • G01N2458/10Oligonucleotides as tagging agents for labelling antibodies
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Chemical Kinetics & Catalysis (AREA)
AU2014278537A 2013-06-12 2014-06-03 Methods, kits, and systems for multiplexed detection of target molecules and uses thereof Active AU2014278537B2 (en)

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AU2018204228A AU2018204228B2 (en) 2013-06-12 2018-06-14 Methods, kits, and systems for multiplexed detection of target molecules and uses thereof
AU2020204460A AU2020204460B2 (en) 2013-06-12 2020-07-03 Methods, kits, and systems for multiplexed detection of target molecules and uses thereof

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US201361834111P 2013-06-12 2013-06-12
US61/834,111 2013-06-12
US201361912054P 2013-12-05 2013-12-05
US61/912,054 2013-12-05
US201461972940P 2014-03-31 2014-03-31
US61/972,940 2014-03-31
PCT/US2014/040731 WO2014200767A1 (en) 2013-06-12 2014-06-03 Methods, kits, and systems for multiplexed detection of target molecules and uses thereof

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US (2) US10266874B2 (cg-RX-API-DMAC7.html)
EP (2) EP3008201B1 (cg-RX-API-DMAC7.html)
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CA (1) CA2915033C (cg-RX-API-DMAC7.html)
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Families Citing this family (140)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
US10787701B2 (en) 2010-04-05 2020-09-29 Prognosys Biosciences, Inc. Spatially encoded biological assays
US20190300945A1 (en) 2010-04-05 2019-10-03 Prognosys Biosciences, Inc. Spatially Encoded Biological Assays
RS54482B1 (sr) 2010-04-05 2016-06-30 Prognosys Biosciences, Inc. Biološka testiranja sa prostornim kodiranjem
US10941396B2 (en) 2012-02-27 2021-03-09 Becton, Dickinson And Company Compositions and kits for molecular counting
BR112015003354A8 (pt) 2012-08-14 2018-01-16 10X Genomics Inc métodos e composições de microcápsula
US10584381B2 (en) 2012-08-14 2020-03-10 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9951386B2 (en) 2014-06-26 2018-04-24 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10273541B2 (en) 2012-08-14 2019-04-30 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10323279B2 (en) 2012-08-14 2019-06-18 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10752949B2 (en) 2012-08-14 2020-08-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9701998B2 (en) 2012-12-14 2017-07-11 10X Genomics, Inc. Methods and systems for processing polynucleotides
US11591637B2 (en) 2012-08-14 2023-02-28 10X Genomics, Inc. Compositions and methods for sample processing
EP3901280B1 (en) 2012-10-17 2025-03-12 10x Genomics Sweden AB Methods and product for optimising localised or spatial detection of gene expression in a tissue sample
US10533221B2 (en) 2012-12-14 2020-01-14 10X Genomics, Inc. Methods and systems for processing polynucleotides
EP3567116A1 (en) 2012-12-14 2019-11-13 10X Genomics, Inc. Methods and systems for processing polynucleotides
CA2900481A1 (en) 2013-02-08 2014-08-14 10X Genomics, Inc. Polynucleotide barcode generation
US10130711B2 (en) 2013-06-19 2018-11-20 The Regents Of The University Of California Chemical structures for localized delivery of therapeutic agents
LT3013983T (lt) 2013-06-25 2023-05-10 Prognosys Biosciences, Inc. Erdviniai koduoti biologiniai tyrimai, naudojant mikrofluidinį įrenginį
EP3039158B1 (en) 2013-08-28 2018-11-14 Cellular Research, Inc. Massively parallel single cell analysis
EP3116525B1 (en) 2014-03-14 2021-06-23 The Regents of the University of California Tco conjugates and methods for delivery of therapeutic agents
KR102596508B1 (ko) 2014-04-10 2023-10-30 10엑스 제노믹스, 인크. 시약을 캡슐화 및 구획화하기 위한 유체 디바이스, 시스템, 및 방법, 및 이의 응용
US9909167B2 (en) 2014-06-23 2018-03-06 The Board Of Trustees Of The Leland Stanford Junior University On-slide staining by primer extension
US12312640B2 (en) 2014-06-26 2025-05-27 10X Genomics, Inc. Analysis of nucleic acid sequences
EP4574974A3 (en) 2014-06-26 2025-10-08 10x Genomics, Inc. Methods of analyzing nucleic acids from individual cells or cell populations
KR20170026383A (ko) 2014-06-26 2017-03-08 10엑스 제노믹스, 인크. 핵산 서열의 분석
SG11201705615UA (en) 2015-01-12 2017-08-30 10X Genomics Inc Processes and systems for preparing nucleic acid sequencing libraries and libraries prepared using same
US10697000B2 (en) 2015-02-24 2020-06-30 10X Genomics, Inc. Partition processing methods and systems
US9727810B2 (en) 2015-02-27 2017-08-08 Cellular Research, Inc. Spatially addressable molecular barcoding
JP7508191B2 (ja) 2015-03-30 2024-07-01 ベクトン・ディキンソン・アンド・カンパニー コンビナトリアルバーコーディングのための方法および組成物
HK1250245A1 (zh) 2015-04-10 2018-12-07 Spatial Transcriptomics Ab 生物样本的空间区别、多重核酸分析
WO2016172373A1 (en) 2015-04-23 2016-10-27 Cellular Research, Inc. Methods and compositions for whole transcriptome amplification
AU2016297513B2 (en) * 2015-07-17 2021-02-25 Board Of Regents, The University Of Texas System Simultaneous quantification of a plurality of proteins in a user-defined region of a cross-sectioned tissue
ES2975362T3 (es) 2015-07-17 2024-07-04 Nanostring Technologies Inc Cuantificación simultánea de la expresión génica en una región definida por el usuario de un tejido seccionado transversalmente
US10828373B2 (en) 2015-09-10 2020-11-10 Tambo, Inc. Bioorthogonal compositions
KR102395450B1 (ko) 2015-09-11 2022-05-09 셀룰러 리서치, 인크. 핵산 라이브러리 정규화를 위한 방법 및 조성물
US11371094B2 (en) 2015-11-19 2022-06-28 10X Genomics, Inc. Systems and methods for nucleic acid processing using degenerate nucleotides
EP4144861B1 (en) 2015-12-04 2024-09-11 10X Genomics, Inc. Methods and compositions for nucleic acid analysis
JP6920334B2 (ja) * 2016-03-25 2021-08-18 ザ ジェネラル ホスピタル コーポレイション 偏光に基づく蛍光核酸検出
US10301677B2 (en) 2016-05-25 2019-05-28 Cellular Research, Inc. Normalization of nucleic acid libraries
US10202641B2 (en) 2016-05-31 2019-02-12 Cellular Research, Inc. Error correction in amplification of samples
US10640763B2 (en) 2016-05-31 2020-05-05 Cellular Research, Inc. Molecular indexing of internal sequences
JP6940532B2 (ja) * 2016-06-30 2021-09-29 バイオイズ カンパニー リミテッド 二本鎖核酸信号プローブおよびこれを用いた標的分子の検出方法
WO2018017469A1 (en) * 2016-07-18 2018-01-25 Yale University Multiplex detection of intracellular or surface molecular targets in single cells
CA3031586A1 (en) 2016-07-27 2018-02-01 The Board Of Trustees Of The Leland Stanford Junior University Highly-multiplexed fluorescent imaging
EP3516400B1 (en) * 2016-09-26 2023-08-16 Becton, Dickinson and Company Measurement of protein expression using reagents with barcoded oligonucleotide sequences
US11667951B2 (en) 2016-10-24 2023-06-06 Geneinfosec, Inc. Concealing information present within nucleic acids
CN110121364A (zh) * 2016-12-19 2019-08-13 文塔纳医疗系统公司 肽核酸缀合物
US10011872B1 (en) 2016-12-22 2018-07-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10550429B2 (en) 2016-12-22 2020-02-04 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10815525B2 (en) 2016-12-22 2020-10-27 10X Genomics, Inc. Methods and systems for processing polynucleotides
EP4310183B1 (en) 2017-01-30 2025-07-09 10X Genomics, Inc. Methods and systems for droplet-based single cell barcoding
US11319583B2 (en) 2017-02-01 2022-05-03 Becton, Dickinson And Company Selective amplification using blocking oligonucleotides
US10995333B2 (en) 2017-02-06 2021-05-04 10X Genomics, Inc. Systems and methods for nucleic acid preparation
WO2018148700A1 (en) 2017-02-13 2018-08-16 Yale University High-throughput single-cell polyomics
EP3606559B1 (en) 2017-04-07 2024-06-05 Tambo, Inc. Bioorthogonal compositions
EP3620529A4 (en) * 2017-05-02 2021-02-24 The University of Tokyo PROCESS FOR MONITORING DYNAMIC CHANGES IN CELLS OR A SUBSTANCE DERIVED THEREOF, AND PROCESS FOR CELL CLASSIFICATION USING THIS MONITORING PROCEDURE
US20180340169A1 (en) 2017-05-26 2018-11-29 10X Genomics, Inc. Single cell analysis of transposase accessible chromatin
CN109526228B (zh) 2017-05-26 2022-11-25 10X基因组学有限公司 转座酶可接近性染色质的单细胞分析
JP7536450B2 (ja) 2017-06-05 2024-08-20 ベクトン・ディキンソン・アンド・カンパニー 単一細胞用のサンプルインデックス付加
WO2019060857A1 (en) * 2017-09-25 2019-03-28 Plexium, Inc. CHEMICAL LIBRARIES CODED BY OLIGONUCLEOTIDES
US20200256862A1 (en) * 2017-10-23 2020-08-13 Massachusetts Institute Of Technology Functionalized solid support
WO2019099751A1 (en) 2017-11-15 2019-05-23 10X Genomics, Inc. Functionalized gel beads
US10829815B2 (en) 2017-11-17 2020-11-10 10X Genomics, Inc. Methods and systems for associating physical and genetic properties of biological particles
WO2019118355A1 (en) * 2017-12-12 2019-06-20 10X Genomics, Inc. Systems and methods for single cell processing
WO2019120635A1 (en) 2017-12-18 2019-06-27 Ventana Medical Systems, Inc. Peptide nucleic acid conjugates
JP7158642B2 (ja) * 2018-01-16 2022-10-24 国立大学法人山梨大学 質量分析装置及び質量分析システム
KR101911859B1 (ko) * 2018-02-02 2018-12-28 주식회사 바이오이즈 이중가닥 핵산 신호 프로브 및 이를 이용한 표적분자의 검출방법
KR102879375B1 (ko) 2018-02-12 2025-10-30 브루커 스페이셜 바이올로지, 인크. 유전자 및 단백질 발현을 검출하는 생체 분자 프로브 및 방법
CN112005115A (zh) 2018-02-12 2020-11-27 10X基因组学有限公司 表征来自单个细胞或细胞群体的多种分析物的方法
SG11202009889VA (en) 2018-04-06 2020-11-27 10X Genomics Inc Systems and methods for quality control in single cell processing
CN118910215A (zh) 2018-05-03 2024-11-08 贝克顿迪金森公司 在相对的转录物末端进行分子条形码化
CN112272710A (zh) 2018-05-03 2021-01-26 贝克顿迪金森公司 高通量多组学样品分析
CN110456034B (zh) * 2018-05-07 2022-07-22 上海市第十人民医院 一种循环肿瘤细胞的检测方法
WO2019217758A1 (en) 2018-05-10 2019-11-14 10X Genomics, Inc. Methods and systems for molecular library generation
EP3797171B1 (en) * 2018-05-21 2025-08-27 The Regents of The University of California Single cell mapping and transcriptome analysis
US20200020419A1 (en) * 2018-07-16 2020-01-16 Flagship Pioneering Innovations Vi, Llc. Methods of analyzing cells
US20200032335A1 (en) 2018-07-27 2020-01-30 10X Genomics, Inc. Systems and methods for metabolome analysis
US11639517B2 (en) 2018-10-01 2023-05-02 Becton, Dickinson And Company Determining 5′ transcript sequences
US11932849B2 (en) 2018-11-08 2024-03-19 Becton, Dickinson And Company Whole transcriptome analysis of single cells using random priming
US11459607B1 (en) 2018-12-10 2022-10-04 10X Genomics, Inc. Systems and methods for processing-nucleic acid molecules from a single cell using sequential co-partitioning and composite barcodes
EP4567127A3 (en) 2018-12-10 2025-09-03 10x Genomics, Inc. Methods for determining a location of a biological analyte in a biological sample
CN113195717A (zh) 2018-12-13 2021-07-30 贝克顿迪金森公司 单细胞全转录组分析中的选择性延伸
US11926867B2 (en) 2019-01-06 2024-03-12 10X Genomics, Inc. Generating capture probes for spatial analysis
US11649485B2 (en) 2019-01-06 2023-05-16 10X Genomics, Inc. Generating capture probes for spatial analysis
US11845983B1 (en) 2019-01-09 2023-12-19 10X Genomics, Inc. Methods and systems for multiplexing of droplet based assays
EP4242322B1 (en) 2019-01-23 2024-08-21 Becton, Dickinson and Company Oligonucleotides associated with antibodies
WO2020185312A2 (en) 2019-01-25 2020-09-17 President And Fellows Of Harvard College Compositions and method for synthesizing nucleic acids
US11851683B1 (en) 2019-02-12 2023-12-26 10X Genomics, Inc. Methods and systems for selective analysis of cellular samples
US12071617B2 (en) 2019-02-14 2024-08-27 Becton, Dickinson And Company Hybrid targeted and whole transcriptome amplification
CN114174531A (zh) 2019-02-28 2022-03-11 10X基因组学有限公司 用空间条码化寡核苷酸阵列对生物分析物进行概况分析
CN114127309A (zh) 2019-03-15 2022-03-01 10X基因组学有限公司 使用空间阵列进行单细胞测序的方法
EP3887542A1 (en) 2019-03-22 2021-10-06 10X Genomics, Inc. Three-dimensional spatial analysis
JP7183899B2 (ja) * 2019-03-25 2022-12-06 株式会社Jvcケンウッド 分析装置及び分析方法
CN113874727A (zh) * 2019-03-27 2021-12-31 富鲁达加拿大公司 冷冻干燥的抗体组
WO2020214642A1 (en) 2019-04-19 2020-10-22 Becton, Dickinson And Company Methods of associating phenotypical data and single cell sequencing data
EP3976820A1 (en) 2019-05-30 2022-04-06 10X Genomics, Inc. Methods of detecting spatial heterogeneity of a biological sample
US11939622B2 (en) 2019-07-22 2024-03-26 Becton, Dickinson And Company Single cell chromatin immunoprecipitation sequencing assay
KR102406463B1 (ko) * 2019-09-03 2022-06-10 재단법인 오송첨단의료산업진흥재단 단백질 생산성 향상을 위한 개별 바코딩 시스템 도입한 시그널 펩타이드 초고속 선별방법
US12235262B1 (en) 2019-09-09 2025-02-25 10X Genomics, Inc. Methods and systems for single cell protein analysis
JP7522189B2 (ja) 2019-11-08 2024-07-24 ベクトン・ディキンソン・アンド・カンパニー 免疫レパートリーシーケンシングのための完全長v(d)j情報を得るためのランダムプライミングの使用
EP4055185A1 (en) 2019-11-08 2022-09-14 10X Genomics, Inc. Spatially-tagged analyte capture agents for analyte multiplexing
EP4058598B1 (en) 2019-11-13 2025-04-23 10X Genomics, Inc. Generating capture probes for spatial analysis
WO2021119402A1 (en) 2019-12-12 2021-06-17 President And Fellows Of Harvard College Compositions and methods for light-directed biomolecular barcoding
WO2021146207A1 (en) 2020-01-13 2021-07-22 Becton, Dickinson And Company Methods and compositions for quantitation of proteins and rna
ES2993319T3 (en) 2020-01-29 2024-12-27 Becton Dickinson Co Barcoded wells for spatial mapping of single cells through sequencing
US12399123B1 (en) 2020-02-14 2025-08-26 10X Genomics, Inc. Spatial targeting of analytes
JP7647736B2 (ja) * 2020-02-14 2025-03-18 ソニーグループ株式会社 分析方法、分析システム、及び分析用表面
US12281357B1 (en) 2020-02-14 2025-04-22 10X Genomics, Inc. In situ spatial barcoding
CN115151810A (zh) 2020-02-25 2022-10-04 贝克顿迪金森公司 实现使用单细胞样品作为单色补偿对照的双特异性探针
US20240018607A1 (en) * 2020-03-31 2024-01-18 Logicink Corporation Molecular beacons
CN115997030A (zh) 2020-04-30 2023-04-21 德迈逊科技有限公司 用于大分子操作的装置和方法
CN115605614A (zh) 2020-05-14 2023-01-13 贝克顿迪金森公司(Us) 用于免疫组库谱分析的引物
WO2021243285A1 (en) * 2020-05-29 2021-12-02 Seer, Inc. Systems and methods for rapid identification of proteins
CN115803445A (zh) 2020-06-02 2023-03-14 贝克顿迪金森公司 用于5撇基因表达测定的寡核苷酸和珠
EP4162074B1 (en) 2020-06-08 2024-04-24 10X Genomics, Inc. Methods of determining a surgical margin and methods of use thereof
US11981960B1 (en) 2020-07-06 2024-05-14 10X Genomics, Inc. Spatial analysis utilizing degradable hydrogels
US11932901B2 (en) 2020-07-13 2024-03-19 Becton, Dickinson And Company Target enrichment using nucleic acid probes for scRNAseq
WO2022026909A1 (en) 2020-07-31 2022-02-03 Becton, Dickinson And Company Single cell assay for transposase-accessible chromatin
WO2022109343A1 (en) 2020-11-20 2022-05-27 Becton, Dickinson And Company Profiling of highly expressed and lowly expressed proteins
JPWO2022118862A1 (cg-RX-API-DMAC7.html) * 2020-12-01 2022-06-09
US12392771B2 (en) 2020-12-15 2025-08-19 Becton, Dickinson And Company Single cell secretome analysis
EP4121555A1 (en) 2020-12-21 2023-01-25 10X Genomics, Inc. Methods, compositions, and systems for capturing probes and/or barcodes
US20240076723A1 (en) 2020-12-30 2024-03-07 10X Genomics, Inc. Cleavage of capture probes for spatial analysis
AU2022238446A1 (en) 2021-03-18 2023-09-07 10X Genomics, Inc. Multiplex capture of gene and protein expression from a biological sample
EP4341848A1 (en) * 2021-05-19 2024-03-27 Leica Microsystems CMS GmbH Marker, method and device for analyzing a biological sample
EP4341847A1 (en) * 2021-05-19 2024-03-27 Leica Microsystems CMS GmbH Marker, method and device for analyzing a biological sample
WO2022256503A1 (en) 2021-06-03 2022-12-08 10X Genomics, Inc. Methods, compositions, kits, and systems for enhancing analyte capture for spatial analysis
EP4396581A1 (en) * 2021-08-31 2024-07-10 Becton, Dickinson and Company Multiplexed proteomics analysis using oligonucleotide-conjugated antibodies
EP4305195A2 (en) 2021-12-01 2024-01-17 10X Genomics, Inc. Methods, compositions, and systems for improved in situ detection of analytes and spatial analysis
US20250144110A1 (en) * 2022-01-28 2025-05-08 Washington University Compositions of autophagy modulating agents and uses thereof
WO2023250077A1 (en) 2022-06-22 2023-12-28 10X Genomics, Inc. Methods, compositions, and systems for capturing probes and/or barcodes
EP4343326B1 (en) * 2022-09-20 2025-03-12 Miltenyi Biotec B.V. & Co. KG Biological conjugates having an photogenerated acidic or basic releasable detection moiety on top of biological tissues
WO2024064866A2 (en) * 2022-09-22 2024-03-28 Astrin Biosciences, Inc. Tumor cell profiling-based therapeutic targeting of cancer
US20240118269A1 (en) * 2022-09-28 2024-04-11 Massachusetts Institute Of Technology High Throughput Stochastic Bio-Molecular Sensor
WO2024145491A1 (en) 2022-12-30 2024-07-04 10X Genomics, Inc. Methods, compositions, and kits for multiple barcoding and/or high-density spatial barcoding
KR102869006B1 (ko) * 2023-03-14 2025-10-14 재단법인 아산사회복지재단 다중면역형광염색을 이용한 세포 시료에서 표적 단백질을 검출하는 방법
WO2025049491A1 (en) * 2023-08-29 2025-03-06 Genopheno, Llc. Novel sampling and genetic comparison and analysis of cannabis components for commercial differentiation
WO2025188805A1 (en) * 2024-03-04 2025-09-12 University Of Denver Blood based rapid diagnostic system for brain hemorrhage detection and associated methods
WO2025226877A1 (en) * 2024-04-23 2025-10-30 Delfi Diagnostics, Inc. Workflows for discovery and deployment of diagnostic assays combining proteomic and genomic information

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011143583A1 (en) * 2010-05-13 2011-11-17 Illumina, Inc. Binding assays for markers

Family Cites Families (49)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NL154598B (nl) 1970-11-10 1977-09-15 Organon Nv Werkwijze voor het aantonen en bepalen van laagmoleculire verbindingen en van eiwitten die deze verbindingen specifiek kunnen binden, alsmede testverpakking.
US3817837A (en) 1971-05-14 1974-06-18 Syva Corp Enzyme amplification assay
US3939350A (en) 1974-04-29 1976-02-17 Board Of Trustees Of The Leland Stanford Junior University Fluorescent immunoassay employing total reflection for activation
US3996345A (en) 1974-08-12 1976-12-07 Syva Company Fluorescence quenching with immunological pairs in immunoassays
US4275149A (en) 1978-11-24 1981-06-23 Syva Company Macromolecular environment control in specific receptor assays
US4277437A (en) 1978-04-05 1981-07-07 Syva Company Kit for carrying out chemically induced fluorescence immunoassay
US4366241A (en) 1980-08-07 1982-12-28 Syva Company Concentrating zone method in heterogeneous immunoassays
US5093268A (en) 1988-04-28 1992-03-03 Igen, Inc. Apparatus for conducting a plurality of simultaneous measurements of electrochemiluminescent phenomena
DE3920358A1 (de) 1989-06-22 1991-01-17 Behringwerke Ag Bispezifische und oligospezifische, mono- und oligovalente antikoerperkonstrukte, ihre herstellung und verwendung
AU663053B2 (en) 1990-06-11 1995-09-28 Gilead Sciences, Inc. Nucleic acid ligands
DK1136556T3 (da) 1991-11-25 2005-10-03 Enzon Inc Fremgangsmåde til fremstilling af multivalente antigen-bindende proteiner
US6136540A (en) 1994-10-03 2000-10-24 Ikonisys Inc. Automated fluorescence in situ hybridization detection of genetic abnormalities
US5430175A (en) 1993-11-30 1995-07-04 Cornell Research Foundation, Inc. Caged carboxyl compounds and use thereof
US5888829A (en) 1994-11-08 1999-03-30 Molecular Probes, Inc. Photolabile caged ionophores and method of using in a membrane separation process
US5635608A (en) 1994-11-08 1997-06-03 Molecular Probes, Inc. α-carboxy caged compounds
CN1661115A (zh) 1995-03-10 2005-08-31 梅索磅秤技术有限公司 多阵列、多特异性的电化学发光检验
US5641870A (en) 1995-04-20 1997-06-24 Genentech, Inc. Low pH hydrophobic interaction chromatography for antibody purification
US5872243A (en) 1995-06-30 1999-02-16 Molecular Probes, Inc. Caged nucleotides
US6043065A (en) 1997-12-23 2000-03-28 University Of Maryland Biotechnology Institute Photosensitive organic compounds that release 2,5,-di(tert-butyl)hydroquinone upon illumination
US6472541B2 (en) 1998-11-20 2002-10-29 The Regents Of The University Of California Protecting groups with increased photosensitivities
US20070009931A1 (en) * 2000-08-04 2007-01-11 Kirsch Wolff M Negative correlation between IRP-2 and Transferrin receptor expression as a diagnostic of Alzheimer's disease
US6958216B2 (en) 2001-01-10 2005-10-25 The Trustees Of Boston College DNA-bridged carbon nanotube arrays
US6960437B2 (en) 2001-04-06 2005-11-01 California Institute Of Technology Nucleic acid amplification utilizing microfluidic devices
WO2003048295A1 (en) 2001-11-30 2003-06-12 Fluidigm Corporation Microfluidic device and methods of using same
US20030194711A1 (en) 2002-04-10 2003-10-16 Matthew Zapala System and method for analyzing gene expression data
JP2006504105A (ja) 2002-10-28 2006-02-02 ユニバーシティ オブ ワシントン 波長調節可能な表面プラズモン共振センサ
US20050079520A1 (en) * 2003-07-21 2005-04-14 Jie Wu Multiplexed analyte detection
AU2005301593A1 (en) * 2004-11-01 2006-05-11 Synthera Technologies Co., Ltd. Method of detecting target substances
US8986926B2 (en) 2005-12-23 2015-03-24 Nanostring Technologies, Inc. Compositions comprising oriented, immobilized macromolecules and methods for their preparation
EP1963531B1 (en) 2005-12-23 2011-09-21 Nanostring Technologies, Inc. Nanoreporters and methods of manufacturing and use thereof
ES2620398T3 (es) 2006-05-22 2017-06-28 Nanostring Technologies, Inc. Sistemas y métodos para analizar nanoindicadores
JP5555157B2 (ja) * 2007-04-10 2014-07-23 ナノストリング テクノロジーズ, インコーポレイテッド ナノレポーターにおける使用のための標的特異的配列を同定するための方法およびコンピュータシステム
US8865401B2 (en) * 2007-12-14 2014-10-21 The Johns Hopkins University Purification and concentration of proteins and DNA from a complex sample using isotachophoresis and a device to perform the purification
US7923562B2 (en) * 2008-06-16 2011-04-12 The Board Of Trustees Of The Leland Stanford Junior University Photocleavable linker methods and compositions
US9000142B2 (en) 2008-07-07 2015-04-07 Syntrix Biosystems, Inc. Photocleavable sense-antisense complex
JP5836803B2 (ja) 2008-08-14 2015-12-24 ナノストリング テクノロジーズ, インコーポレイテッド 安定したナノレポーター
EP2380025B1 (en) * 2009-01-14 2013-09-11 The United States of America, as represented by The Secretary, Department of Health and Human Services Ratio based biomarkers and methods of use thereof
CA2775613C (en) 2009-10-13 2018-06-12 Nanostring Technologies, Inc. Protein detection via nanoreporters
WO2011097644A2 (en) * 2010-02-08 2011-08-11 Isis Pharmaceuticals, Inc. Selective reduction of allelic variants
EP2516681B1 (en) * 2010-02-11 2017-10-18 Nanostring Technologies, Inc Compositions and methods for the detection of preferably small rnas by bridge hybridisation and ligation
EP2547795B1 (en) 2010-03-16 2015-01-14 Nanostring Technologies, Inc Compositions and methods for the detection of genomic features
US9110079B2 (en) * 2010-09-29 2015-08-18 Biomerieux Method and kit for establishing an in vitro prognosis on a patient exhibiting SIRS
WO2012135340A2 (en) 2011-03-28 2012-10-04 Nanostring Technologies, Inc. Compositions and methods for diagnosing cancer
JP2013027387A (ja) * 2011-06-21 2013-02-07 Tohoku Univ 膵臓癌バイオマーカー
US20130017971A1 (en) 2011-06-24 2013-01-17 Nanostring Technologies, Inc. Multivariate Diagnostic Assays and Methods for Using Same
EP2769225A4 (en) * 2011-10-17 2015-06-10 Nuclea Biotechnologies Inc BIOLOGICAL MARKERS PREDICTIVE FOR BREAST CANCER
WO2013078332A1 (en) 2011-11-23 2013-05-30 The General Hospital Corporation Analyte detection using magnetic hall effect
US20140037620A1 (en) 2012-06-29 2014-02-06 British Columbia Cancer Agency Branch Methods of Treating Breast Cancer with Gemcitabine Therapy
EP2976642A4 (en) * 2013-03-15 2016-09-21 Harvard College METHOD AND COMPOSITIONS FOR IMPROVING THE DETECTION AND / OR RECORDING OF A TARGET UNIT

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011143583A1 (en) * 2010-05-13 2011-11-17 Illumina, Inc. Binding assays for markers

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
AGASTI ET AL., "Photocleavable DNA Barcode-Antibody Conjugates Allow Sensitive and Multiplexed Protein Analysis in Single Cells", JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, (20121023), vol. 134, pages 18499 - 18502 *

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