AU2014277874B2 - Methods of producing omega-hydroxylated fatty acid derivatives - Google Patents

Methods of producing omega-hydroxylated fatty acid derivatives Download PDF

Info

Publication number
AU2014277874B2
AU2014277874B2 AU2014277874A AU2014277874A AU2014277874B2 AU 2014277874 B2 AU2014277874 B2 AU 2014277874B2 AU 2014277874 A AU2014277874 A AU 2014277874A AU 2014277874 A AU2014277874 A AU 2014277874A AU 2014277874 B2 AU2014277874 B2 AU 2014277874B2
Authority
AU
Australia
Prior art keywords
fatty acid
hydroxy
seq
recombinant microorganism
cyp153a
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
AU2014277874A
Other languages
English (en)
Other versions
AU2014277874A1 (en
Inventor
Cindy Chang
Stephen B. Del Cardayre
Louis G. HOM
Zhihao Hu
Emanuela E. Popova
Andreas W. Schirmer
Haibo Wang
Baolong Zhu
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Genomatica Inc
Original Assignee
Genomatica Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genomatica Inc filed Critical Genomatica Inc
Publication of AU2014277874A1 publication Critical patent/AU2014277874A1/en
Application granted granted Critical
Publication of AU2014277874B2 publication Critical patent/AU2014277874B2/en
Priority to AU2020294235A priority Critical patent/AU2020294235B2/en
Assigned to GENOMATICA, INC. reassignment GENOMATICA, INC. Request for Assignment Assignors: REG Life Sciences, LLC
Ceased legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0008—Oxidoreductases (1.) acting on the aldehyde or oxo group of donors (1.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0077—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14) with a reduced iron-sulfur protein as one donor (1.14.15)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/18—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic polyhydric
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/42—Hydroxy-carboxylic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y114/00—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14)
    • C12Y114/14—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14) with reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen (1.14.14)
    • C12Y114/14001—Unspecific monooxygenase (1.14.14.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y114/00—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14)
    • C12Y114/15—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14) with reduced iron-sulfur protein as one donor, and incorporation of one atom of oxygen (1.14.15)
    • C12Y114/15003—Alkane 1-monooxygenase (1.14.15.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y203/00—Acyltransferases (2.3)
    • C12Y203/01—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C12Y203/0102—Diacylglycerol O-acyltransferase (2.3.1.20)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y203/00—Acyltransferases (2.3)
    • C12Y203/01—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C12Y203/01075—Long-chain-alcohol O-fatty-acyltransferase (2.3.1.75)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/01—Carboxylic ester hydrolases (3.1.1)
    • C12Y301/01005—Lysophospholipase (3.1.1.5)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/02—Thioester hydrolases (3.1.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/02—Thioester hydrolases (3.1.2)
    • C12Y301/02014—Oleoyl-[acyl-carrier-protein] hydrolase (3.1.2.14), i.e. ACP-thioesterase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y102/00—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2)
    • C12Y102/01—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2) with NAD+ or NADP+ as acceptor (1.2.1)
    • C12Y102/01042—Hexadecanal dehydrogenase (acylating) (1.2.1.42), i.e. fatty acyl-CoA reductase
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
AU2014277874A 2013-06-14 2014-06-16 Methods of producing omega-hydroxylated fatty acid derivatives Ceased AU2014277874B2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2020294235A AU2020294235B2 (en) 2013-06-14 2020-12-23 Methods of producing omega-hydroxylated fatty acid derivatives

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201361835464P 2013-06-14 2013-06-14
US61/835,464 2013-06-14
PCT/US2014/042594 WO2014201474A1 (en) 2013-06-14 2014-06-16 Methods of producing omega-hydroxylated fatty acid derivatives

Related Child Applications (1)

Application Number Title Priority Date Filing Date
AU2020294235A Division AU2020294235B2 (en) 2013-06-14 2020-12-23 Methods of producing omega-hydroxylated fatty acid derivatives

Publications (2)

Publication Number Publication Date
AU2014277874A1 AU2014277874A1 (en) 2016-02-04
AU2014277874B2 true AU2014277874B2 (en) 2020-12-03

Family

ID=51177165

Family Applications (2)

Application Number Title Priority Date Filing Date
AU2014277874A Ceased AU2014277874B2 (en) 2013-06-14 2014-06-16 Methods of producing omega-hydroxylated fatty acid derivatives
AU2020294235A Active AU2020294235B2 (en) 2013-06-14 2020-12-23 Methods of producing omega-hydroxylated fatty acid derivatives

Family Applications After (1)

Application Number Title Priority Date Filing Date
AU2020294235A Active AU2020294235B2 (en) 2013-06-14 2020-12-23 Methods of producing omega-hydroxylated fatty acid derivatives

Country Status (12)

Country Link
US (3) US10711288B2 (cg-RX-API-DMAC7.html)
EP (2) EP3745133A1 (cg-RX-API-DMAC7.html)
JP (3) JP7004500B2 (cg-RX-API-DMAC7.html)
KR (4) KR20240049856A (cg-RX-API-DMAC7.html)
CN (3) CN118374462A (cg-RX-API-DMAC7.html)
AU (2) AU2014277874B2 (cg-RX-API-DMAC7.html)
BR (2) BR122020002919B1 (cg-RX-API-DMAC7.html)
CA (1) CA2915229A1 (cg-RX-API-DMAC7.html)
MX (2) MX374736B (cg-RX-API-DMAC7.html)
MY (3) MY207701A (cg-RX-API-DMAC7.html)
SI (1) SI2984490T1 (cg-RX-API-DMAC7.html)
WO (1) WO2014201474A1 (cg-RX-API-DMAC7.html)

Families Citing this family (31)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MY207701A (en) 2013-06-14 2025-03-13 Genomatica Inc Methods of producing omega-hydroxylated fatty acid derivatives
AU2015243186C1 (en) 2014-04-10 2020-04-02 Genomatica, Inc. Semisynthetic routes to organic compounds
WO2015176020A1 (en) 2014-05-16 2015-11-19 Provivi, Inc. Synthesis of olefinic alcohols via enzymatic terminal hydroxylation
MX372803B (es) * 2014-06-16 2020-07-03 Genomatica Inc Polipeptidos de fusion relacionados con omega-hidroxilasa con propiedades mejoradas.
BR112017001105A2 (pt) 2014-07-18 2018-07-24 REG Life Sciences, LLC produção microbiana de dióis graxos
US11319562B2 (en) 2015-04-15 2022-05-03 Ramon Gonzalez Modified fatty acid biosynthesis with ACP-dependent thiolases
EP3283618A4 (en) * 2015-04-15 2018-12-05 William Marsh Rice University Modified fatty acid biosynthesis with acp-dependent thiolases
KR101903551B1 (ko) 2015-10-27 2018-10-04 한국생명공학연구원 중쇄 디올의 생산 방법
KR101903553B1 (ko) * 2015-10-27 2018-10-04 한국생명공학연구원 중쇄 아미노카르복시산의 생산 방법
WO2017101987A1 (en) * 2015-12-15 2017-06-22 REG Life Sciences, LLC Omega-hydroxylase-related fusion polypeptide variants with improved properties
CN107201356A (zh) * 2016-03-17 2017-09-26 中国科学院青岛生物能源与过程研究所 支撑p450脂肪酸脱羧酶活性的还原伴侣蛋白组合及其应用
KR102677413B1 (ko) 2017-04-03 2024-06-24 게노마티카 인코포레이티드 중쇄 지방산 유도체의 생산을 위해 개선된 활성을 갖는 티오에스테라제 변이체
KR102170282B1 (ko) * 2017-12-15 2020-10-29 건국대학교 산학협력단 α,ω-디올류 생산하는 재조합 미생물 및 α,ω-디올류의 생산방법
TWI701040B (zh) * 2018-03-19 2020-08-11 國立臺灣大學 蛋白質的用途及包含該蛋白質之全細胞催化系統及其應用
EP3792351A4 (en) 2018-04-16 2022-01-19 Korea Research Institute of Bioscience and Biotechnology Method for producing multi-hydroxy derivatives of polyunsaturated fatty acids
CN112424338B (zh) * 2018-07-17 2025-09-02 科纳根公司 γ-内酯的生物合成制备
KR102117828B1 (ko) 2018-07-19 2020-06-02 서울대학교산학협력단 레귤론 변이를 통한 지방산 및 지방산 유도체 생산에서 불포화도 감소
EP3844179A4 (en) 2018-08-31 2022-08-31 Genomatica, Inc. XYLR MUTANT FOR IMPROVED XYLOSE UTILIZATION OR IMPROVED CO-UTILIZATION OF GLUCOSE AND XYLOSE
CN111218430B (zh) * 2018-11-26 2022-06-14 中国科学院大连化学物理研究所 一种nadh类似物依赖型的细胞色素p450还原酶及其应用
CN111218485B (zh) * 2018-11-26 2023-01-24 中国科学院大连化学物理研究所 一种生产ω-1-羟基脂肪酸的方法
CN111592996A (zh) * 2019-12-09 2020-08-28 福建省水产研究所（福建水产病害防治中心） 一种芽孢杆菌cqn-2高密度发酵培养方法
WO2022212119A1 (en) * 2021-03-29 2022-10-06 Genomatica, Inc. Production of non-native monounsaturated fatty acids in bacteria
JP2023005283A (ja) * 2021-06-28 2023-01-18 出光興産株式会社 ペプチドタグ付加タンパク質を用いた代謝物生産
EP4493675A1 (en) 2022-03-16 2025-01-22 Genomatica, Inc. Microorganisms and methods for production of fatty acid derivatives with reduced levels of byproducts
CN114480513A (zh) * 2022-04-01 2022-05-13 北京林业大学 一种基因编辑酵母体外合成麝香酮的方法
CN115537369B (zh) * 2022-11-09 2024-03-01 云南农业大学 2-十二烯二酸促进蒙氏假单胞菌生长和定殖的应用
CN116064362B (zh) * 2022-12-15 2025-10-28 浙江大学 一种利用葡萄糖从头合成12-氨基月桂酸的基因工程菌及其构建和应用
CN116790529B (zh) * 2023-05-24 2025-01-17 天津大学 P450 bm3蛋白突变体及其应用
CN117343970B (zh) * 2023-10-24 2025-01-21 杭州佳嘉乐生物技术有限公司 一种提升植物油脂亲肤感的油脂发酵方法
CN120699859A (zh) * 2024-11-13 2025-09-26 中国科学院深圳先进技术研究院 重组菌及其构建方法和应用
CN120254135B (zh) * 2025-06-04 2025-08-29 中国农业科学院北京畜牧兽医研究所 一种高精度、抗干扰的内毒素分析方法及试剂盒

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012071439A1 (en) * 2010-11-22 2012-05-31 The Regents Of The University Of California Host cells and methods for producing diacid compounds

Family Cites Families (62)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1305083C (en) 1987-02-18 1992-07-14 Yoshiharu Kimura Microorganism belonging to genus rhodococcus, and a process for producing alkene derivative and unsaturated fatty acid
US5482846A (en) 1988-08-31 1996-01-09 University Of Florida Ethanol production in Gram-positive microbes
US5000000A (en) 1988-08-31 1991-03-19 University Of Florida Ethanol production by Escherichia coli strains co-expressing Zymomonas PDC and ADH genes
US5028539A (en) 1988-08-31 1991-07-02 The University Of Florida Ethanol production using engineered mutant E. coli
US5424202A (en) 1988-08-31 1995-06-13 The University Of Florida Ethanol production by recombinant hosts
EP0528881A4 (en) 1990-04-24 1993-05-26 Stratagene Methods for phenotype creation from multiple gene populations
US5602030A (en) 1994-03-28 1997-02-11 University Of Florida Research Foundation Recombinant glucose uptake system
US6428767B1 (en) 1995-05-12 2002-08-06 E. I. Du Pont De Nemours And Company Method for identifying the source of carbon in 1,3-propanediol
US5939250A (en) 1995-12-07 1999-08-17 Diversa Corporation Production of enzymes having desired activities by mutagenesis
US5965408A (en) 1996-07-09 1999-10-12 Diversa Corporation Method of DNA reassembly by interrupting synthesis
EP1021551A1 (en) 1997-10-06 2000-07-26 Centre National De La Recherche Scientifique Plant fatty acid hydroxylase genes
EP1501784B1 (en) 2002-04-29 2019-03-13 Dow Global Technologies LLC Integrated chemical processes for industrial utilization of seed oils
US20040242831A1 (en) 2003-05-30 2004-12-02 Dong Tian Enzyme catalyzed polyesters and polyol polymers
JPWO2006051729A1 (ja) 2004-11-10 2008-05-29 株式会社海洋バイオテクノロジー研究所 Ｐ４５０遺伝子の単離法
JP2009079057A (ja) 2005-04-22 2009-04-16 Mitsubishi Chemicals Corp ポリエステル及びその製造方法
US20100242345A1 (en) 2006-05-19 2010-09-30 LS9, Inc Production of fatty acids & derivatives thereof
US8110670B2 (en) 2006-05-19 2012-02-07 Ls9, Inc. Enhanced production of fatty acid derivatives
EP2194119A3 (en) 2006-05-19 2010-09-22 LS9, Inc. Production of fatty acids and derivatives thereof
WO2008140468A2 (en) 2006-10-13 2008-11-20 Elevance Renewable Sciences, Inc. METHODS OF MAKING α, ω -DICARBOXYLIC ACID ALKENE DERIVATIVES BY METATHESIS
CN102123979A (zh) 2006-10-13 2011-07-13 埃莱文斯可更新科学公司 通过烯烃复分解由内烯烃合成末端烯烃的方法
WO2008113041A2 (en) 2007-03-14 2008-09-18 Ls9, Inc. Process for producing low molecular weight hydrocarbons from renewable resources
CA2678915C (en) 2007-03-28 2019-04-09 Ls9, Inc. Enhanced production of fatty acid derivatives
US20080293060A1 (en) 2007-04-23 2008-11-27 Ls9, Inc. Methods and Compositions for Identification of Hydrocarbon Response, Transport and Biosynthesis Genes
CA2679085C (en) 2007-05-22 2018-04-10 Ls9, Inc. Hydrocarbon-producing genes and methods of their use
JP5324129B2 (ja) * 2007-05-25 2013-10-23 神戸天然物化学株式会社 Ｃｙｐ１５３による芳香族化合物の製造方法
WO2009009391A2 (en) 2007-07-06 2009-01-15 Ls9, Inc. Systems and methods for the production of fatty esters
FR2921362B1 (fr) 2007-09-20 2012-09-21 Arkema France Procede de synthese d'acides gras omega-insatures
CA2692266C (en) 2007-09-27 2019-04-16 Ls9, Inc. Reduction of the toxic effect of impurities from raw materials by extractive fermentation
TWI351779B (en) 2007-12-03 2011-11-01 Advance Smart Ind Ltd Apparatus and method for correcting residual capac
WO2009085278A1 (en) 2007-12-21 2009-07-09 Ls9, Inc. Methods and compositions for producing olefins
CN102027108A (zh) 2008-05-16 2011-04-20 Ls9公司 产生碳氢化合物的方法和组合物
US8232924B2 (en) 2008-05-23 2012-07-31 Alliant Techsystems Inc. Broadband patch antenna and antenna system
US8273694B2 (en) 2008-07-28 2012-09-25 Jeffrey A Brown Synthetic compositions obtained from algae
US20100071259A1 (en) 2008-08-18 2010-03-25 Ls9, Inc. Systems and methods for production of mixed fatty esters
TW201016657A (en) 2008-08-28 2010-05-01 Mitsui Chemicals Inc Olefin production process
CA3038491A1 (en) 2008-10-07 2010-04-15 REG Life Sciences, LLC Bacterial host cells engineered to express a carboxylic acid reductase and a thioesterase
EP2796559B1 (en) 2008-10-28 2018-09-12 REG Life Sciences, LLC Microbial cell for producing fatty alcohols
US7989875B2 (en) 2008-11-24 2011-08-02 Nxp B.V. BiCMOS integration of multiple-times-programmable non-volatile memories
US9175234B2 (en) 2008-12-23 2015-11-03 REG Life Sciences, LLC Methods and compositions related to thioesterase enzymes
US8071799B2 (en) 2009-01-29 2011-12-06 Energy & Environmental Research Center Foundation Chain-selective synthesis of fuel components and chemical feedstocks
JP5353768B2 (ja) 2009-03-06 2013-11-27 国立大学法人東京工業大学 樹脂組成物
BRPI1014952A2 (pt) 2009-04-10 2019-09-24 Ls9 Inc "composição produzida por um microrganismo e composição de biocombustível."
BRPI1015313B1 (pt) 2009-04-27 2021-06-01 Genomatica, Inc. Método fermentativo para produzir um éster de ácido graxo empregando um microorganismo geneticamente modificado e método para a produção de ésteres de ácidos graxos
WO2010127318A2 (en) 2009-05-01 2010-11-04 The Regents Of The University Of California Product of fatty acid esters from biomass polymers
ES2707747T3 (es) 2009-09-25 2019-04-04 Reg Life Sciences Llc Producción de derivados de ácidos grasos
US8237003B2 (en) 2009-11-09 2012-08-07 Exxonmobil Chemical Patents Inc. Metathesis catalyst and process for use thereof
US8809563B2 (en) 2009-11-09 2014-08-19 Exxonmobil Chemical Patents Inc. Metathesis catalyst and process for use thereof
US8361769B1 (en) 2009-11-16 2013-01-29 U.S. Department Of Energy Regioselective alkane hydroxylation with a mutant CYP153A6 enzyme
US20110124071A1 (en) 2009-11-17 2011-05-26 LS9, Inc Methods and compositions for producing hydrocarbons
US8530221B2 (en) 2010-01-14 2013-09-10 Ls9, Inc. Production of branched chain fatty acids and derivatives thereof in recombinant microbial cells
US20110250663A1 (en) * 2010-04-08 2011-10-13 Ls9, Inc. Methods and compositions related to fatty alcohol biosynthetic enzymes
JP5868962B2 (ja) 2010-05-28 2016-02-24 ソラザイム, インコーポレイテッドSolazyme Inc 組み換え従属栄養性微生物から産生された用途に応じた油
US8372610B2 (en) 2010-09-15 2013-02-12 Ls9, Inc. Production of odd chain fatty acid derivatives in recombinant microbial cells
JP2012097163A (ja) * 2010-10-29 2012-05-24 Dainippon Printing Co Ltd ポリエステル樹脂組成物
MX351063B (es) 2011-02-02 2017-09-29 Terravia Holdings Inc Aceites adaptados producidos a partir de microorganismos oleaginosos recombinantes.
MY176185A (en) * 2011-07-06 2020-07-24 Radici Chimica S P A Biological methods for preparing a fatty dicarboxylic acid
DE102011110946A1 (de) 2011-08-15 2016-01-21 Evonik Degussa Gmbh Biotechnologisches Syntheseverfahren von omegafunktionalisierten Carbonsäuren und Carbonsäure-Estern aus einfachen Kohlenstoffquellen
EP2639308A1 (de) 2012-03-12 2013-09-18 Evonik Industries AG Enzymatische omega-Oxidation und -Aminierung von Fettsäuren
KR20230026539A (ko) 2012-10-15 2023-02-24 게노마티카 인코포레이티드 특정 길이의 지방 알콜 및 관련 화합물의 생산을 위한 미생물 및 방법
FR3003577B1 (fr) 2013-03-19 2016-05-06 Ferropem Inoculant a particules de surface
MY207701A (en) 2013-06-14 2025-03-13 Genomatica Inc Methods of producing omega-hydroxylated fatty acid derivatives
MX372803B (es) 2014-06-16 2020-07-03 Genomatica Inc Polipeptidos de fusion relacionados con omega-hidroxilasa con propiedades mejoradas.

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012071439A1 (en) * 2010-11-22 2012-05-31 The Regents Of The University Of California Host cells and methods for producing diacid compounds

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
MÉLANIE BORDEAUX ET AL, "A Regioselective Biocatalyst for Alkane Activation under Mild Conditions", ANGEWANDTE CHEMIE INTERNATIONAL EDITION, (2011-01-24), vol. 50, no. 9, doi:10.1002/anie.201005597, ISSN 1433-7851, pages 2075 - 2079 *
MIHO NODATE ET AL, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, SPRINGER, BERLIN, DE, (2005-09-30), vol. 71, no. 4, ISSN 1432-0614, pages 455 - 462 *
MITSUTOSHI KUBOTA ET AL, BIOSCIENCE, BIOTECHNOLOGY, AND BIOCHEMISTRY, (2005-01-01), vol. 69, no. 12, doi:10.1271/bbb.69.2421, ISSN 0916-8451, pages 2421 - 2430 *

Also Published As

Publication number Publication date
CN105378486A (zh) 2016-03-02
KR20220053681A (ko) 2022-04-29
JP2021176340A (ja) 2021-11-11
EP2984490A1 (en) 2016-02-17
JP2020022473A (ja) 2020-02-13
SI2984490T1 (sl) 2020-10-30
WO2014201474A1 (en) 2014-12-18
CN118374462A (zh) 2024-07-23
EP3745133A1 (en) 2020-12-02
AU2020294235A1 (en) 2021-01-28
KR20240049856A (ko) 2024-04-17
KR102256678B1 (ko) 2021-05-28
MX374736B (es) 2025-03-06
US20160130616A1 (en) 2016-05-12
MX385154B (es) 2025-03-14
CA2915229A1 (en) 2014-12-18
US20210071212A1 (en) 2021-03-11
JP7168539B2 (ja) 2022-11-09
JP7004500B2 (ja) 2022-02-04
MY197101A (en) 2023-05-24
CN105378486B (zh) 2024-05-14
KR20160020516A (ko) 2016-02-23
MY207701A (en) 2025-03-13
MY203897A (en) 2024-07-23
JP2016521577A (ja) 2016-07-25
AU2020294235B2 (en) 2023-06-08
JP7406529B2 (ja) 2023-12-27
MX2015016947A (es) 2016-04-25
BR112015031233A2 (pt) 2017-12-19
US10711288B2 (en) 2020-07-14
EP2984490B1 (en) 2020-05-20
KR20210060678A (ko) 2021-05-26
US20250019731A1 (en) 2025-01-16
CN118460487A (zh) 2024-08-09
AU2014277874A1 (en) 2016-02-04
KR102387905B1 (ko) 2022-04-18
BR122020002919B1 (pt) 2023-03-21
US11981952B2 (en) 2024-05-14

Similar Documents

Publication Publication Date Title
AU2020294235B2 (en) Methods of producing omega-hydroxylated fatty acid derivatives
US20230167419A1 (en) Omega-Hydroxylase-Related Fusion Polypeptides With Improved Properties
US12180515B2 (en) Omega-hydroxylase-related fusion polypeptide variants with improved properties

Legal Events

Date Code Title Description
FGA Letters patent sealed or granted (standard patent)
PC Assignment registered

Owner name: GENOMATICA, INC.

Free format text: FORMER OWNER(S): REG LIFE SCIENCES, LLC

MK14 Patent ceased section 143(a) (annual fees not paid) or expired