AU2013256174B2 - Viability staining method - Google Patents
Viability staining method Download PDFInfo
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- AU2013256174B2 AU2013256174B2 AU2013256174A AU2013256174A AU2013256174B2 AU 2013256174 B2 AU2013256174 B2 AU 2013256174B2 AU 2013256174 A AU2013256174 A AU 2013256174A AU 2013256174 A AU2013256174 A AU 2013256174A AU 2013256174 B2 AU2013256174 B2 AU 2013256174B2
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- cells
- fluorescent
- membrane
- viable
- viable cells
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- AWDBHOZBRXWRKS-UHFFFAOYSA-N tetrapotassium;iron(6+);hexacyanide Chemical compound [K+].[K+].[K+].[K+].[Fe+6].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-] AWDBHOZBRXWRKS-UHFFFAOYSA-N 0.000 description 1
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
- C12Q1/06—Quantitative determination
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6486—Measuring fluorescence of biological material, e.g. DNA, RNA, cells
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
- G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
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- Cell Biology (AREA)
- Medicinal Chemistry (AREA)
- Food Science & Technology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Applications Claiming Priority (5)
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| US201261641805P | 2012-05-02 | 2012-05-02 | |
| US61/641,805 | 2012-05-02 | ||
| US201361784759P | 2013-03-14 | 2013-03-14 | |
| US61/784,759 | 2013-03-14 | ||
| PCT/US2013/039347 WO2013166336A1 (en) | 2012-05-02 | 2013-05-02 | Viability staining method |
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| AU2013256174A1 AU2013256174A1 (en) | 2014-11-20 |
| AU2013256174B2 true AU2013256174B2 (en) | 2017-03-02 |
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| AU2013256174A Active AU2013256174B2 (en) | 2012-05-02 | 2013-05-02 | Viability staining method |
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| JP (1) | JP6285915B2 (enExample) |
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| CA (1) | CA2872149C (enExample) |
| WO (1) | WO2013166336A1 (enExample) |
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| BR112013002667B1 (pt) | 2010-08-04 | 2020-02-04 | Thomas Julius Borody | composições para transplante da flora intestinal e métodos para fazê-las e usá-las e dispositivos para administrá-las |
| EP3610881A1 (en) | 2011-03-09 | 2020-02-19 | Regents Of The University Of Minnesota | Compositions and methods for transplantation of colon microbiota |
| JP6482459B2 (ja) | 2012-05-02 | 2019-03-13 | チャールズ リバー ラボラトリーズ， インコーポレイテッド | 細胞サンプル中の生存細胞を検出する方法 |
| CN104685346B (zh) | 2012-05-02 | 2018-05-29 | 查尔斯河实验室公司 | 细胞捕获系统及其使用 |
| US8993260B2 (en) | 2012-05-02 | 2015-03-31 | Charles River Laboratories, Inc. | Fluorescence-based viability staining method using a membrane permeable flourescent dye and membrane impermeable fluorescence quencher |
| US9719144B2 (en) | 2012-05-25 | 2017-08-01 | Arizona Board Of Regents | Microbiome markers and therapies for autism spectrum disorders |
| WO2015066727A1 (en) * | 2013-11-04 | 2015-05-07 | Charles River Laboratories, Inc. | Method for detecting viable cells in a cell sample |
| EP3066189B1 (en) | 2013-11-04 | 2019-09-18 | Charles River Laboratories, Inc. | Filtration system and use thereof |
| US20150218612A1 (en) * | 2014-02-06 | 2015-08-06 | Tacount Exact Ltd. | Apparatus, system and method for live bacteria microscopy |
| WO2016053931A1 (en) * | 2014-09-30 | 2016-04-07 | Corning Incorporated | Membrane with improved fluorescent spectral blocking efficiency |
| CN107209115A (zh) | 2015-01-26 | 2017-09-26 | 百克特瑞欧扫描有限责任公司 | 具有转盘式流体样品装置的激光散射测量仪器 |
| WO2016161337A1 (en) * | 2015-04-03 | 2016-10-06 | Captl Llc | Particle detection using reflective surface |
| CN107949391B (zh) * | 2015-05-14 | 2021-11-09 | 克雷斯顿沃控股公司 | 用于粪便菌群移植的组合物以及用于制备和使用它们的方法和用于递送它们的装置 |
| PT3297644T (pt) | 2015-05-22 | 2022-03-30 | Univ Arizona State | Métodos para tratar distúrbios do espetro do autismo e sintomas associados |
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| US10613096B2 (en) | 2015-08-28 | 2020-04-07 | Captl Llc | Multi-spectral microparticle-fluorescence photon cytometry |
| JP6660712B2 (ja) | 2015-11-10 | 2020-03-11 | 株式会社Ｓｃｒｅｅｎホールディングス | 分類器構成方法および細胞の生死判定方法 |
| US20170360848A1 (en) | 2016-06-15 | 2017-12-21 | Arizona Board Of Regents On Behalf Of Arizona State University | Methods for treating autism spectrum disorder and associated symptoms |
| US10849936B2 (en) | 2016-07-01 | 2020-12-01 | Regents Of The University Of Minnesota | Compositions and methods for C. difficile treatment |
| US20180036352A1 (en) | 2016-08-03 | 2018-02-08 | Crestovo Holdings Llc | Methods for treating ulcerative colitis |
| US11213549B2 (en) | 2016-10-11 | 2022-01-04 | Finch Therapeutics Holdings Llc | Compositions and method for treating primary sclerosing cholangitis and related disorders |
| US10092601B2 (en) | 2016-10-11 | 2018-10-09 | Crestovo Holdings Llc | Compositions and methods for treating multiple sclerosis and related disorders |
| WO2018071537A1 (en) | 2016-10-11 | 2018-04-19 | Crestovo Holdings Llc | Compositions and methods for treating multiple sclerosis and related disorders |
| WO2018187464A1 (en) | 2017-04-05 | 2018-10-11 | Crestovo Holdings Llc | Compositions and methods for treating diverticulitis and related disorders |
| WO2018187467A1 (en) | 2017-04-05 | 2018-10-11 | Crestovo Holdings Llc | Compositions and methods for treating parkinson's disease (pd) and related disorders |
| US11187584B2 (en) | 2017-04-13 | 2021-11-30 | Captl Llc | Photon counting and spectroscopy |
| USD829338S1 (en) * | 2017-05-15 | 2018-09-25 | Charles River Laboratories, Inc. | Membrane holder |
| WO2018218159A1 (en) | 2017-05-26 | 2018-11-29 | Crestovo Holdings Llc | Lyophilized compositions comprising fecal microbe-based therapeutic agents and methods for making and using same |
| US11865145B2 (en) | 2017-08-07 | 2024-01-09 | Finch Therapeutics Holdings Llc | Compositions and methods for maintaining and restoring a healthy gut barrier |
| GB201716883D0 (en) * | 2017-10-13 | 2017-11-29 | Q-Linea Ab | Method for determining the concentration of intact microorganisms in a sample |
| JP2021501322A (ja) * | 2017-10-26 | 2021-01-14 | エッセンリックス コーポレーション | 血小板の高速測定法 |
| JP6627069B2 (ja) | 2018-06-01 | 2020-01-08 | 株式会社フロンティアファーマ | 画像処理方法、薬剤感受性試験方法および画像処理装置 |
| EP3805401B1 (en) | 2018-06-01 | 2023-09-27 | Frontier Pharma, Inc. | Image processing method, medicine sensitivity test method and image processing device |
| US11166990B2 (en) | 2018-07-13 | 2021-11-09 | Finch Therapeutics Holdings Llc | Methods and compositions for treating ulcerative colitis |
| MX2020014054A (es) * | 2018-07-20 | 2021-05-27 | Illumina Inc | Composicion de resina y celdas de flujo que incluyen la misma. |
| CN113164527A (zh) | 2018-09-27 | 2021-07-23 | 芬奇治疗控股有限责任公司 | 用于治疗癫痫和相关障碍的组合物和方法 |
| US10564100B1 (en) * | 2018-10-12 | 2020-02-18 | SageMedic Corporation | Analysis of viable and nonviable cells |
| PE20221399A1 (es) | 2019-07-19 | 2022-09-15 | Finch Therapeutics Holdings Llc | Metodos y productos para el tratamiento de trastornos gastrointestinales |
| JP2023521428A (ja) * | 2020-04-15 | 2023-05-24 | ラピッド マイクロ バイオシステムズ インコーポレイテッド | バックグラウンド減弱型微生物学栄養培地及びその使用方法 |
| US20250146044A1 (en) * | 2023-11-06 | 2025-05-08 | Rapid Micro Biosystems, Inc. | Rinse solution for microbial detection systems |
Family Cites Families (112)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA1256360A (en) | 1984-02-10 | 1989-06-27 | John W. Bornhoeft | Apparatus and method for determining the effectiveness of sterlization |
| EP0215066A1 (en) | 1985-02-27 | 1987-03-25 | University Of Cincinnati | Viable microorganism detection by induced fluorescence |
| US4739416A (en) | 1986-09-15 | 1988-04-19 | Matrix Instruments Inc. | Digital image reproduction |
| US4725891A (en) | 1986-09-15 | 1988-02-16 | Matrix Instruments Inc. | Film image digitizer |
| US4783401A (en) | 1986-10-31 | 1988-11-08 | Smithkline Beckman Corporation | Viable cell labelling |
| JPH0813930B2 (ja) | 1987-12-28 | 1996-02-14 | 三井東圧化学株式会社 | 近赤外線吸収フィルター用高純度アントラキノン系色素 |
| US4930893A (en) | 1988-03-25 | 1990-06-05 | Molecular Dynamics | Electrophoresis imaging system |
| US4838632A (en) | 1988-05-06 | 1989-06-13 | Lumisys Inc. | Two-dimensional beam scanner |
| US5073488A (en) | 1988-11-29 | 1991-12-17 | Minnesota Mining And Manufacturing Company | Rapid method for determining efficacy of a sterilization cycle and rapid read-out biological indicator |
| WO1992002632A1 (en) | 1990-07-30 | 1992-02-20 | Sierra Cytometry | Fluorescent dyes for identification and enumeration of viable cells in milk |
| US5185450A (en) | 1991-02-19 | 1993-02-09 | University Of South Florida | Tetrazolium compounds for cell viability assays |
| US5172419A (en) | 1991-03-05 | 1992-12-15 | Lumisys, Inc. | Medical image processing system |
| US5296341A (en) | 1991-03-05 | 1994-03-22 | Lumisys, Inc. | Green light laser imaging method |
| US5314805A (en) | 1991-10-28 | 1994-05-24 | Molecular Probes, Inc. | Dual-fluorescence cell viability assay using ethidium homodimer and calcein AM |
| US5221454A (en) | 1992-01-31 | 1993-06-22 | Biometric Imaging Inc. | Differential separation assay |
| US5843680A (en) | 1992-01-31 | 1998-12-01 | Biometric Imaging, Inc. | Differential separation assay methods and test kits |
| US5137609A (en) | 1992-01-31 | 1992-08-11 | Biometric Imaging Inc. | Differential separation assay |
| DE69311944T2 (de) | 1992-04-01 | 1997-11-20 | Nihon Millipore Kogyo K K | Verfahren zur Bestimmung der Anzahl lebender Mikroorganismen |
| JP3228812B2 (ja) | 1993-02-10 | 2001-11-12 | 日本マイクロリス株式会社 | 生菌数を測定する方法 |
| US5585246A (en) | 1993-02-17 | 1996-12-17 | Biometric Imaging, Inc. | Method for preparing a sample in a scan capillary for immunofluorescent interrogation |
| US5547849A (en) | 1993-02-17 | 1996-08-20 | Biometric Imaging, Inc. | Apparatus and method for volumetric capillary cytometry |
| US5658751A (en) | 1993-04-13 | 1997-08-19 | Molecular Probes, Inc. | Substituted unsymmetrical cyanine dyes with selected permeability |
| US5534416A (en) | 1993-04-13 | 1996-07-09 | Molecular Probes, Inc. | Fluorescent viability assay using cyclic-substituted unsymmetrical cyanine dyes |
| US5545535A (en) | 1993-04-13 | 1996-08-13 | Molecular Probes, Inc. | Fluorescent assay for bacterial gram reaction |
| US5436134A (en) | 1993-04-13 | 1995-07-25 | Molecular Probes, Inc. | Cyclic-substituted unsymmetrical cyanine dyes |
| US5445946A (en) | 1993-04-13 | 1995-08-29 | Molecular Probes, Inc. | Intravacuolar stains for yeast and other fungi |
| US5437980A (en) | 1993-05-17 | 1995-08-01 | Molecular Probes, Inc. | Phenanthridium dye staining of nucleic acids in living cells |
| FR2707665A1 (fr) | 1993-06-28 | 1995-01-20 | Chemunex | Procédé de mesure et d'évaluation de la vitalité des microorganismes et ses applications. |
| IES940182A2 (en) | 1994-03-01 | 1995-11-29 | Teagasc Agric Food Dev Authori | "Rapid detection of bacteria in liquid cultures" |
| US5550032A (en) | 1994-05-27 | 1996-08-27 | George Mason University | Biological assay for microbial contamination |
| EP0713087B1 (en) | 1994-11-17 | 1999-04-14 | Chemunex | Apparatus and process for rapid and ultrasensitive detection and counting of microorganisms by fluorescence |
| US5603900A (en) | 1995-05-19 | 1997-02-18 | Millipore Investment Holdings Limited | Vacuum filter device |
| US5565678A (en) | 1995-06-06 | 1996-10-15 | Lumisys, Inc. | Radiographic image quality assessment utilizing a stepped calibration target |
| WO1997012226A1 (en) | 1995-09-25 | 1997-04-03 | Tencor Instruments | Improved system for surface inspection |
| HUP9902028A2 (hu) | 1995-11-08 | 1999-11-29 | Pioneer Hi-Bred International, Inc. | Eljárások mikrobatenyészetek életképességének becslésére |
| US20020119438A1 (en) | 1995-12-29 | 2002-08-29 | Fuminori Kato | Method for determining viable cell count |
| US6459805B1 (en) * | 1996-03-26 | 2002-10-01 | Childrens Hospital Los Angeles | Fluorescence digital imaging microscopy system |
| DE19621312A1 (de) | 1996-05-28 | 1997-12-04 | Bayer Ag | Maskierung der Hintergrundfluoreszenz und Signalverstärkung bei der optischen Analyse biologisch medizinischer Assays |
| US5956146A (en) | 1997-01-29 | 1999-09-21 | Victor Company Of Japan, Ltd. | Birefringence measuring apparatus for optical disc substrate |
| US6221621B1 (en) | 1997-03-06 | 2001-04-24 | Bard Diagnostic Sciences, Inc. | Methods of screening for colorectal cancers in which a complement Factor I or related protein is associated |
| FR2764305B1 (fr) | 1997-06-04 | 2000-10-06 | Chemunex | Procede de detection et de numeration de cellules viables dans un echantillon biologique et kit pour sa mise en oeuvre |
| US6200762B1 (en) | 1997-08-01 | 2001-03-13 | Aurora Biosciences Corporation | Photon reducing agents and compositions for fluorescence assays |
| US6214563B1 (en) | 1997-08-01 | 2001-04-10 | Aurora Biosciences Corporation | Photon reducing agents for reducing undesired light emission in assays |
| US6221612B1 (en) | 1997-08-01 | 2001-04-24 | Aurora Biosciences Corporation | Photon reducing agents for use in fluorescence assays |
| US6043506A (en) | 1997-08-13 | 2000-03-28 | Bio-Rad Laboratories, Inc. | Multi parameter scanner |
| US5922617A (en) | 1997-11-12 | 1999-07-13 | Functional Genetics, Inc. | Rapid screening assay methods and devices |
| WO1999035499A1 (en) | 1997-12-30 | 1999-07-15 | Remacle Jose | Method comprising capture molecule fixed on disc surface |
| US6080868A (en) | 1998-01-23 | 2000-06-27 | The Perkin-Elmer Corporation | Nitro-substituted non-fluorescent asymmetric cyanine dye compounds |
| JP2002521690A (ja) | 1998-07-28 | 2002-07-16 | バイオメトリック イメージング インコーポレイテッド | 細胞運動性アッセイのための装置および方法 |
| US6130745A (en) | 1999-01-07 | 2000-10-10 | Biometric Imaging, Inc. | Optical autofocus for use with microtiter plates |
| EP1314980B1 (en) | 1999-02-26 | 2009-12-09 | Cellomics, Inc. | A system for cell-based screening |
| JP2000304688A (ja) | 1999-04-16 | 2000-11-02 | Canon Inc | 基板測定方法および装置 |
| US6181413B1 (en) | 1999-09-03 | 2001-01-30 | Biometric Imaging, Inc. | Displacing volume in field of view |
| ATE399878T1 (de) | 1999-10-25 | 2008-07-15 | Genprime Inc | Verfahren und vorrichtung zur quantifizierung von prokaryotischen und eukaryotischen zellen |
| JP2001228088A (ja) | 2000-02-18 | 2001-08-24 | Nippon Laser & Electronics Lab | 生体試料光学的走査装置 |
| JP2001242082A (ja) | 2000-02-29 | 2001-09-07 | Nippon Laser & Electronics Lab | 生体試料光学的走査装置 |
| GB0008787D0 (en) | 2000-04-10 | 2000-05-31 | Norsk Naeringsmiddelforskning | A method of cell detection |
| US6323337B1 (en) | 2000-05-12 | 2001-11-27 | Molecular Probes, Inc. | Quenching oligonucleotides |
| US7118878B1 (en) | 2000-06-09 | 2006-10-10 | Promega Corporation | Method for increasing luminescence assay sensitivity |
| IL139593A (en) | 2000-11-09 | 2010-12-30 | Biogem Optical Ltd | Method for the detection of viable microorganisms |
| US6714287B2 (en) | 2001-01-02 | 2004-03-30 | Becton, Dickinson And Company | Apparatus for determining the volume of single red blood cells |
| US6633368B2 (en) | 2001-01-02 | 2003-10-14 | Becton, Dickinson And Company | Method for determining the volume of single red blood cells |
| CA2445509A1 (en) | 2001-01-25 | 2002-12-19 | James D. Thacker | Rapid methods for microbial typing and enumeration |
| US7016087B2 (en) | 2001-08-08 | 2006-03-21 | Becton Dickinson And Company | Photon efficient scanner |
| US6750457B2 (en) | 2001-08-29 | 2004-06-15 | Becton Dickinson And Company | System for high throughput analysis |
| CA2458802C (en) | 2001-09-06 | 2016-06-07 | Genomic Profiling Systems, Inc. | Rapid and sensitive detection of molecules |
| US7205100B2 (en) | 2002-10-10 | 2007-04-17 | Advanced Analytical Technologies, Inc. | Methods for reducing background fluorescence |
| ES2239886B1 (es) | 2003-11-05 | 2006-12-16 | Universidad De Barcelona | Metodo y aparato para la determinacion de la viabilidad celular. |
| EP1699351B1 (en) | 2003-12-22 | 2011-05-04 | Lightouch Medical, Inc. | Process for determination of cell viability |
| WO2005088287A1 (en) | 2004-03-17 | 2005-09-22 | National Research Council Of Canada | Method and apparatus for the detection of microorganisms |
| US20060129327A1 (en) | 2004-07-29 | 2006-06-15 | Kim Myung L | Ultrasensitive sensor and rapid detection of analytes |
| DE602005010515D1 (de) | 2004-08-06 | 2008-12-04 | Fuji Electric Holdings | Nachweisverfahren für lebenfähige Zellen |
| US20060121443A1 (en) | 2004-12-03 | 2006-06-08 | Orion Biosolutions, Inc. | Determination of cell viability |
| US7018804B1 (en) | 2004-12-03 | 2006-03-28 | Orion Biosolutions, Inc. | Determination of cell viability and phenotype |
| US20090170144A1 (en) | 2005-02-22 | 2009-07-02 | Heineman William R | Determination of viable microorganisms using coated paramagnetic beads |
| US8268567B2 (en) | 2005-09-16 | 2012-09-18 | The Charles Stark Draper Laboratory | Reaction sensing in living cells |
| JP5268175B2 (ja) | 2006-01-12 | 2013-08-21 | バイオセンス テクノロジーズ インク． | 迅速に細胞の生存テストを実行するための方法及び構成物 |
| DE102006022877B4 (de) | 2006-05-15 | 2009-01-29 | Sartorius Stedim Biotech Gmbh | Verfahren zur Bestimmung der Viabilität von Zellen in Zellkulturen |
| GB0615302D0 (en) | 2006-08-01 | 2006-09-13 | Biotrace Internat Plc | Assay Method For The Detection Of Viable Microbial Cells In A Sample |
| US20080153125A1 (en) | 2006-11-10 | 2008-06-26 | Advanced Analytical Technologies, Inc. | Rapid detection of microorganisms in fluids |
| CN101201312A (zh) * | 2006-12-15 | 2008-06-18 | 北京林业大学 | 一种中性红染色法检测藻细胞活性的方法 |
| EP2142665B1 (en) | 2007-04-13 | 2013-11-27 | Promega Corporation | Luminescent live and dead cell assay |
| US20080305514A1 (en) | 2007-06-06 | 2008-12-11 | Alcon Research, Ltd. | Method for detecting microbes |
| WO2009029039A1 (en) | 2007-08-28 | 2009-03-05 | Ridgeview Instruments Ab | Method for measurement of cell viability |
| US8216778B2 (en) | 2007-11-09 | 2012-07-10 | Genzyme Corporation | Methods of measuring cell viability in tissue engineered products |
| US8986946B2 (en) | 2007-12-20 | 2015-03-24 | Nederlandse Organisatie Voor Toegepast-Natuurwetenschappelijk Onderzoek Tno | Real-time method for the detection of viable micro-organisms |
| US20110110861A1 (en) | 2008-05-13 | 2011-05-12 | Mireille Hanna Lahoud | Methods of Detecting Cells with a Disrupted Cell Membrane, Cells Infected with A Pathogen, Dying Cells or Dead Cells |
| US9128097B2 (en) | 2008-07-14 | 2015-09-08 | Chemometec A/S | Method and kit for assessing viable cells |
| CN101344476B (zh) * | 2008-08-20 | 2011-03-16 | 吉林农业大学 | 细菌活的非可培养状态荧光显微镜观察与计数方法 |
| CN102414324B (zh) | 2009-05-04 | 2015-12-02 | 诺华股份有限公司 | 快速无菌微测试 |
| US8148515B1 (en) | 2009-06-02 | 2012-04-03 | Biotium, Inc. | Detection using a dye and a dye modifier |
| WO2010151131A2 (en) | 2009-06-26 | 2010-12-29 | Nederlandse Organisatie Voor Toegepast- Natuurwetenschappelijk Onderzoek Tno | Apparatus for detecting viable microorganisms or spores in a sample and use thereof. |
| CA2768699C (en) | 2009-07-24 | 2017-08-22 | Morinaga Milk Industry Co., Ltd. | Method and kit for detection of live microorganisms |
| CA2781516C (en) | 2010-03-26 | 2016-01-26 | Morinaga Milk Industry Co., Ltd. | Culture medium for specific growth, detection, and enumeration of bifidobacterium breve |
| GB201005939D0 (en) * | 2010-04-09 | 2010-05-26 | Biostatus Ltd | Method of analysing a cell or other biological material containing nucleic acid |
| US20110294206A1 (en) | 2010-05-28 | 2011-12-01 | California Institute Of Technology | Methods and design of membrane filters |
| ES2646413T3 (es) | 2010-06-03 | 2017-12-13 | Basf Se | Detección y recuento de microorganismos |
| US20130210063A1 (en) | 2010-10-13 | 2013-08-15 | Life Technologies Corporation | Compositions and assays for determining cell viability |
| CA2815951A1 (en) | 2010-11-03 | 2012-05-10 | Reametrix Inc. | Measurement system for fluorescent detection, and method therefor |
| CA2816014A1 (en) | 2010-11-03 | 2012-05-10 | Reametrix Inc. | Method and device for fluorescent measurement of samples |
| CA2816017A1 (en) | 2010-11-03 | 2012-05-10 | Reametrix Inc. | Methods for calibrating a raman spectrometer, and spectrometer |
| EP2637787A1 (en) | 2010-11-08 | 2013-09-18 | Reactrix Systems, Inc. | Sample assembly for a measurement device |
| US9446411B2 (en) | 2011-02-24 | 2016-09-20 | Reametrix, Inc. | Sample assembly for a measurement device |
| US8993260B2 (en) | 2012-05-02 | 2015-03-31 | Charles River Laboratories, Inc. | Fluorescence-based viability staining method using a membrane permeable flourescent dye and membrane impermeable fluorescence quencher |
| JP6482459B2 (ja) | 2012-05-02 | 2019-03-13 | チャールズ リバー ラボラトリーズ， インコーポレイテッド | 細胞サンプル中の生存細胞を検出する方法 |
| CN104685346B (zh) | 2012-05-02 | 2018-05-29 | 查尔斯河实验室公司 | 细胞捕获系统及其使用 |
| USD705438S1 (en) | 2013-03-14 | 2014-05-20 | Charles River Laboratories, Inc. | Base |
| USD705439S1 (en) | 2013-03-14 | 2014-05-20 | Charles River Laboratories | Base with membrane and support |
| USD704854S1 (en) | 2013-03-14 | 2014-05-13 | Charles River Laboratories, Inc. | Stage |
| USD698939S1 (en) | 2013-03-14 | 2014-02-04 | Charles River Laboratories, Inc. | Lid |
| USD717460S1 (en) | 2013-03-14 | 2014-11-11 | Reametrix, Inc. | Cell detection system |
| USD717352S1 (en) | 2013-03-14 | 2014-11-11 | Reametrix, Inc. | Chuck |
-
2013
- 2013-05-02 US US13/875,936 patent/US8993260B2/en active Active
- 2013-05-02 US US13/875,914 patent/US8993259B2/en active Active
- 2013-05-02 CN CN201380035736.6A patent/CN104662425B/zh active Active
- 2013-05-02 JP JP2015510464A patent/JP6285915B2/ja active Active
- 2013-05-02 CA CA2872149A patent/CA2872149C/en active Active
- 2013-05-02 EP EP13731180.9A patent/EP2769218B1/en active Active
- 2013-05-02 AU AU2013256174A patent/AU2013256174B2/en active Active
- 2013-05-02 WO PCT/US2013/039347 patent/WO2013166336A1/en not_active Ceased
-
2015
- 2015-02-19 US US14/626,711 patent/US20150368689A1/en not_active Abandoned
- 2015-02-19 US US14/626,715 patent/US20150368690A1/en not_active Abandoned
Non-Patent Citations (2)
| Title |
|---|
| "CyQUANT Direct Cell Proliferation Assay Kit", (20090720), URL: http://tools.invitrogen.com/content/sfs/manuals/mp35011.pdf, (20130801) * |
| LI H ET AL, "A live-cell high-throughput screening assay for identification of fatty acid uptake inhibitors", ANALYTICAL BIOCHEMISTRY, , vol. 336, no. 1, doi:10.1016/J.AB.2004.09.025, ISSN 0003-2697, pages 11 - 19 * |
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| US20150368690A1 (en) | 2015-12-24 |
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| CN104662425A (zh) | 2015-05-27 |
| CA2872149A1 (en) | 2013-11-07 |
| JP6285915B2 (ja) | 2018-02-28 |
| CN104662425B (zh) | 2017-10-10 |
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