AU2008236595B2 - Delta-8 desaturases and their use in making polyunsaturated fatty acids - Google Patents

Delta-8 desaturases and their use in making polyunsaturated fatty acids Download PDF

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Publication number
AU2008236595B2
AU2008236595B2 AU2008236595A AU2008236595A AU2008236595B2 AU 2008236595 B2 AU2008236595 B2 AU 2008236595B2 AU 2008236595 A AU2008236595 A AU 2008236595A AU 2008236595 A AU2008236595 A AU 2008236595A AU 2008236595 B2 AU2008236595 B2 AU 2008236595B2
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AU
Australia
Prior art keywords
seq
desaturase
host cell
sequence
acid
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Ceased
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AU2008236595A
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English (en)
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AU2008236595A1 (en
Inventor
Howard Glenn Damude
Quinn Qun Zhu
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EIDP Inc
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EI Du Pont de Nemours and Co
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Publication of AU2008236595A1 publication Critical patent/AU2008236595A1/en
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Publication of AU2008236595B2 publication Critical patent/AU2008236595B2/en
Ceased legal-status Critical Current
Anticipated expiration legal-status Critical

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8247—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified lipid metabolism, e.g. seed oil composition
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0083—Miscellaneous (1.14.99)

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Physics & Mathematics (AREA)
  • Nutrition Science (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Fats And Perfumes (AREA)
AU2008236595A 2007-04-10 2008-04-10 Delta-8 desaturases and their use in making polyunsaturated fatty acids Ceased AU2008236595B2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US91083107P 2007-04-10 2007-04-10
US60/910,831 2007-04-10
PCT/US2008/004700 WO2008124194A1 (en) 2007-04-10 2008-04-10 Delta-8 desaturases and their use in making polyunsaturated fatty acids

Publications (2)

Publication Number Publication Date
AU2008236595A1 AU2008236595A1 (en) 2008-10-16
AU2008236595B2 true AU2008236595B2 (en) 2013-06-13

Family

ID=39708314

Family Applications (2)

Application Number Title Priority Date Filing Date
AU2008236595A Ceased AU2008236595B2 (en) 2007-04-10 2008-04-10 Delta-8 desaturases and their use in making polyunsaturated fatty acids
AU2008237065A Abandoned AU2008237065A1 (en) 2007-04-10 2008-04-10 Delta-8 desaturases and their use in making polyunsaturated fatty acids

Family Applications After (1)

Application Number Title Priority Date Filing Date
AU2008237065A Abandoned AU2008237065A1 (en) 2007-04-10 2008-04-10 Delta-8 desaturases and their use in making polyunsaturated fatty acids

Country Status (9)

Country Link
US (5) US7790156B2 (enExample)
EP (2) EP2132317B1 (enExample)
JP (1) JP5501952B2 (enExample)
CN (1) CN101679990B (enExample)
AU (2) AU2008236595B2 (enExample)
BR (1) BRPI0808222A2 (enExample)
CA (2) CA2681145C (enExample)
DK (1) DK2132317T3 (enExample)
WO (2) WO2008124194A1 (enExample)

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US7790156B2 (en) 2007-04-10 2010-09-07 E. I. Du Pont De Nemours And Company Δ-8 desaturases and their use in making polyunsaturated fatty acids
US8119860B2 (en) * 2007-04-16 2012-02-21 E. I. Du Pont De Nemours And Company Delta-9 elongases and their use in making polyunsaturated fatty acids
EP2195415A1 (en) * 2007-10-03 2010-06-16 E. I. du Pont de Nemours and Company Optimized strains of yarrowia lipolytica for high eicosapentaenoic acid production
AU2010260234B2 (en) 2009-06-16 2015-02-26 E. I. Du Pont De Nemours And Company High eicosapentaenoic acid oils from improved optimized strains of Yarrowia lipolytica
WO2011054801A1 (en) 2009-11-03 2011-05-12 Dsm Ip Assets B.V. Vegatable oil comprising a polyunsaturaded fatty acid having at least 20 carbon atoms
US20130129902A1 (en) 2009-11-03 2013-05-23 Dsm Ip Assets B.V. Composition comprising cells and a polyunsaturated fatty acid having at least 20 carbon atoms (lc-pufa)
US20110263709A1 (en) 2010-04-22 2011-10-27 E. I. Du Pont De Nemours And Company Method for obtaining polyunsaturated fatty acid-containing compositions from microbial biomass
AU2011293167B2 (en) 2010-08-26 2016-12-08 E. I. Du Pont De Nemours And Company Mutant delta-9 elongases and their use in making polyunsaturated fatty acids
AU2011293189B2 (en) 2010-08-26 2017-02-16 E. I. Du Pont De Nemours And Company Mutant HPGG motif and HDASH motif delta-5 desaturases and their use in making polyunsaturated fatty acids
KR20130138760A (ko) 2010-08-26 2013-12-19 이 아이 듀폰 디 네모아 앤드 캄파니 고농도의 에이코사펜타엔산 생성을 위한 재조합 미생물 숙주 세포
WO2012091812A1 (en) 2010-12-30 2012-07-05 E. I. Du Pont De Nemours And Company Use of saccharomyces cerevisiae suc2 gene in yarrowia lipolytica for sucrose utilization
US20130040340A1 (en) 2011-02-07 2013-02-14 E. I. Du Pont De Nemours And Company Production of alcohol esters in situ using alcohols and fatty acids produced by microorganisms
CA2892516A1 (en) 2012-12-21 2014-06-26 E. I. Du Pont De Nemours And Company Down-regulation of a polynucleotide encoding a sou2 sorbitol utilization protein to modify lipid production in microbial cells
UA121847C2 (uk) 2013-03-14 2020-08-10 Піонір Хай-Бред Інтернешнл Інк. Полінуклеотид, що кодує елемент сайленсингу з інсектицидною активністю проти шкідника рослин із ряду coleoptera, та спосіб його застосування
US10023877B2 (en) 2013-03-15 2018-07-17 Pioneer Hi-Bred International, Inc. PHI-4 polypeptides and methods for their use
CN106232820A (zh) 2013-08-16 2016-12-14 先锋国际良种公司 杀昆虫蛋白及其使用方法
US10667524B2 (en) 2013-09-13 2020-06-02 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
PH12016501534B1 (en) 2014-02-07 2024-06-05 Du Pont Insecticidal proteins and methods for their use
CN114763376A (zh) 2014-02-07 2022-07-19 先锋国际良种公司 杀昆虫蛋白及其使用方法
US20170247719A1 (en) 2014-09-17 2017-08-31 Pioneer Hi-Bred International, Inc. Compositions and methods to control insect pests
UA126192C2 (uk) 2014-10-16 2022-08-31 Піонір Хай-Бред Інтернешнл, Інк. Інсектицидний білок та спосіб його застосування
CN114075267B (zh) 2015-01-15 2025-03-18 先锋国际良种公司 杀昆虫蛋白及其使用方法
CN116333064A (zh) 2015-05-19 2023-06-27 先锋国际良种公司 杀昆虫蛋白及其使用方法
CN107771181A (zh) 2015-06-16 2018-03-06 先锋国际良种公司 用以防治昆虫有害生物的组合物和方法
US11198709B2 (en) 2015-08-06 2021-12-14 E. I. Du Pont De Nemours And Company Plant derived insecticidal proteins and methods for their use
JP2018527931A (ja) 2015-08-28 2018-09-27 パイオニア ハイ−ブレッド インターナショナル， インコーポレイテッド 植物のオクロバクテリウム（ｏｃｈｒｏｂａｃｔｒｕｍ）媒介形質転換
CN105137844B (zh) * 2015-08-31 2017-09-01 广东电网有限责任公司电力科学研究院 锅炉调试过程中风险预控方法和系统
EP3390431A1 (en) 2015-12-18 2018-10-24 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
EP3451837B1 (en) 2016-05-04 2021-08-25 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
EP3472323A1 (en) 2016-06-16 2019-04-24 Pioneer Hi-Bred International, Inc. Compositions and methods to control insect pests
MX387506B (es) 2016-07-01 2025-03-18 Pioneer Hi Bred Int Proteinas insecticidas de plantas y metodos para sus usos.
US20210292778A1 (en) 2016-07-12 2021-09-23 Pioneer Hi-Bred International, Inc. Compositions and methods to control insect pests
MX387927B (es) 2016-11-01 2025-03-19 Pioneer Hi Bred Int Proteinas insecticidas y metodos para su uso.
EA201991467A1 (ru) 2016-12-15 2019-11-29 Состав смеси, содержащий силикат и микробные и/или растительные клетки и полиненасыщенную жирную кислоту, имеющую по меньшей мере 20 атомов углерода (lc-pufa)
EP3764798B1 (en) 2018-03-14 2025-12-17 Pioneer Hi-Bred International, Inc. Insecticidal proteins from plants and methods for their use
US11820791B2 (en) 2018-03-14 2023-11-21 Pioneer Hi-Bred International, Inc. Insecticidal proteins from plants and methods for their use
BR112022027035A2 (pt) 2020-07-14 2023-04-11 Pioneer Hi Bred Int Proteínas inseticidas e métodos para uso das mesmas

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WO2000034439A1 (en) * 1998-12-07 2000-06-15 Washington State University Research Foundation Desaturases and methods of using them for synthesis of polyunsaturated fatty acids
US6825017B1 (en) * 1998-12-07 2004-11-30 Washington State University Research Foundation Desaturases and methods of using them for synthesis of polyunsaturated fatty acids
US20060115881A1 (en) * 2004-11-04 2006-06-01 Damude Howard G High eicosapentaenoic acid producing strains of Yarrowia lipolytica

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Also Published As

Publication number Publication date
DK2132317T3 (da) 2013-06-03
EP2121737A2 (en) 2009-11-25
US7790156B2 (en) 2010-09-07
CA2681145A1 (en) 2008-10-16
US8318463B2 (en) 2012-11-27
US20120196347A1 (en) 2012-08-02
EP2121737B1 (en) 2015-05-27
EP2132317B1 (en) 2013-02-27
CA2681145C (en) 2016-02-16
AU2008236595A1 (en) 2008-10-16
WO2008124806A3 (en) 2010-07-22
BRPI0808222A2 (pt) 2014-07-08
JP5501952B2 (ja) 2014-05-28
CN101679990B (zh) 2013-06-19
US20100267099A1 (en) 2010-10-21
US20100099900A1 (en) 2010-04-22
AU2008237065A1 (en) 2008-10-16
US20080254521A1 (en) 2008-10-16
WO2008124806A2 (en) 2008-10-16
JP2010523147A (ja) 2010-07-15
US20080254192A1 (en) 2008-10-16
EP2132317A1 (en) 2009-12-16
CA2683497C (en) 2015-08-11
US8338152B2 (en) 2012-12-25
WO2008124194A1 (en) 2008-10-16
CA2683497A1 (en) 2008-10-16
CN101679990A (zh) 2010-03-24
US8188338B2 (en) 2012-05-29

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