AU2004299457B2 - A human cytotoxic T-lymphocyte epitope and its agonist epitope from the non-variable number of tandem repeat sequence of MUC-1 - Google Patents

Info

Publication number

AU2004299457B2

AU2004299457B2 AU2004299457A AU2004299457A AU2004299457B2 AU 2004299457 B2 AU2004299457 B2 AU 2004299457B2 AU 2004299457 A AU2004299457 A AU 2004299457A AU 2004299457 A AU2004299457 A AU 2004299457A AU 2004299457 B2 AU2004299457 B2 AU 2004299457B2

Authority

AU

Australia

Prior art keywords

cells

muc

cell

peptide

cea

Prior art date

2003-12-12

Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)

Expired

Links

• Espacenet
• Global Dossier
• Discuss

• 108010008707 Mucin-1 Proteins 0.000 title claims description 165
• 102100034256 Mucin-1 Human genes 0.000 title claims description 164
• 210000001151 cytotoxic T lymphocyte Anatomy 0.000 title claims description 36
• 241000282414 Homo sapiens Species 0.000 title description 99
• 239000000556 agonist Substances 0.000 title description 55
• 108090000765 processed proteins & peptides Proteins 0.000 claims description 439
• 210000004027 cell Anatomy 0.000 claims description 423
• 210000004443 dendritic cell Anatomy 0.000 claims description 253
• 102000004196 processed proteins & peptides Human genes 0.000 claims description 232
• 229920001184 polypeptide Polymers 0.000 claims description 154
• 150000007523 nucleic acids Chemical class 0.000 claims description 145
• 239000000427 antigen Substances 0.000 claims description 139
• 108091007433 antigens Proteins 0.000 claims description 136
• 102000036639 antigens Human genes 0.000 claims description 136
• 239000013598 vector Substances 0.000 claims description 135
• 206010028980 Neoplasm Diseases 0.000 claims description 128
• 102000039446 nucleic acids Human genes 0.000 claims description 120
• 108020004707 nucleic acids Proteins 0.000 claims description 120
• 238000000034 method Methods 0.000 claims description 107
• 150000001413 amino acids Chemical class 0.000 claims description 55
• 125000003275 alpha amino acid group Chemical group 0.000 claims description 46
• 201000011510 cancer Diseases 0.000 claims description 45
• 230000028993 immune response Effects 0.000 claims description 45
• 108091028043 Nucleic acid sequence Proteins 0.000 claims description 28
• 210000003819 peripheral blood mononuclear cell Anatomy 0.000 claims description 27
• 230000002163 immunogen Effects 0.000 claims description 20
• 239000013603 viral vector Substances 0.000 claims description 12
• 230000003612 virological effect Effects 0.000 claims description 11
• 239000013612 plasmid Substances 0.000 claims description 10
• 108010022366 Carcinoembryonic Antigen Proteins 0.000 claims description 9
• 230000024932 T cell mediated immunity Effects 0.000 claims description 9
• 230000001939 inductive effect Effects 0.000 claims description 8
• 230000005867 T cell response Effects 0.000 claims description 6
• 230000003213 activating effect Effects 0.000 claims description 4
• 230000004069 differentiation Effects 0.000 claims description 4
• 102000012406 Carcinoembryonic Antigen Human genes 0.000 claims 1
• 210000001744 T-lymphocyte Anatomy 0.000 description 198
• VAOCPAMSLUNLGC-UHFFFAOYSA-N metronidazole Chemical compound CC1=NC=C([N+]([O-])=O)N1CCO VAOCPAMSLUNLGC-UHFFFAOYSA-N 0.000 description 105
• 108090000623 proteins and genes Proteins 0.000 description 104
• 239000002773 nucleotide Substances 0.000 description 69
• 125000003729 nucleotide group Chemical group 0.000 description 69
• 102000004169 proteins and genes Human genes 0.000 description 62
• 235000018102 proteins Nutrition 0.000 description 58
• 102000004127 Cytokines Human genes 0.000 description 55
• 108090000695 Cytokines Proteins 0.000 description 55
• 235000001014 amino acid Nutrition 0.000 description 55
• 108010074032 HLA-A2 Antigen Proteins 0.000 description 54
• 102000025850 HLA-A2 Antigen Human genes 0.000 description 54
• 230000027455 binding Effects 0.000 description 53
• 229940024606 amino acid Drugs 0.000 description 49
• 230000014509 gene expression Effects 0.000 description 49
• 238000004519 manufacturing process Methods 0.000 description 42
• 230000009089 cytolysis Effects 0.000 description 40
• 239000012634 fragment Substances 0.000 description 39
• 102000040430 polynucleotide Human genes 0.000 description 37
• 108091033319 polynucleotide Proteins 0.000 description 37
• 239000002157 polynucleotide Substances 0.000 description 37
• 108020004414 DNA Proteins 0.000 description 36
• 239000002609 medium Substances 0.000 description 36
• 210000000612 antigen-presenting cell Anatomy 0.000 description 35
• 108700018351 Major Histocompatibility Complex Proteins 0.000 description 32
• 238000004458 analytical method Methods 0.000 description 30
• 238000003556 assay Methods 0.000 description 30
• 210000003719 b-lymphocyte Anatomy 0.000 description 30
• 230000020382 suppression by virus of host antigen processing and presentation of peptide antigen via MHC class I Effects 0.000 description 29
• 210000002865 immune cell Anatomy 0.000 description 27
• 208000015181 infectious disease Diseases 0.000 description 27
• 210000004881 tumor cell Anatomy 0.000 description 26
• 238000011282 treatment Methods 0.000 description 25
• 230000001988 toxicity Effects 0.000 description 23
• 231100000419 toxicity Toxicity 0.000 description 23
• 230000002068 genetic effect Effects 0.000 description 22
• 210000000130 stem cell Anatomy 0.000 description 22
• -1 for example Proteins 0.000 description 21
• 108700019146 Transgenes Proteins 0.000 description 20
• 229960005486 vaccine Drugs 0.000 description 20
• 230000000890 antigenic effect Effects 0.000 description 19
• 239000000203 mixture Substances 0.000 description 19
• 239000012636 effector Substances 0.000 description 17
• 230000000694 effects Effects 0.000 description 17
• 239000003623 enhancer Substances 0.000 description 17
• 206010009944 Colon cancer Diseases 0.000 description 16
• 101100346932 Mus musculus Muc1 gene Proteins 0.000 description 16
• 238000001994 activation Methods 0.000 description 16
• 239000002671 adjuvant Substances 0.000 description 16
• 238000000684 flow cytometry Methods 0.000 description 16
• 206010006187 Breast cancer Diseases 0.000 description 15
• 108091026890 Coding region Proteins 0.000 description 15
• 102100039620 Granulocyte-macrophage colony-stimulating factor Human genes 0.000 description 15
• 108060008682 Tumor Necrosis Factor Proteins 0.000 description 15
• 230000004913 activation Effects 0.000 description 15
• 239000003795 chemical substances by application Substances 0.000 description 15
• 210000004698 lymphocyte Anatomy 0.000 description 15
• 230000004048 modification Effects 0.000 description 15
• 238000012986 modification Methods 0.000 description 15
• 208000026310 Breast neoplasm Diseases 0.000 description 14
• 108010017213 Granulocyte-Macrophage Colony-Stimulating Factor Proteins 0.000 description 14
• 230000000139 costimulatory effect Effects 0.000 description 14
• 238000009396 hybridization Methods 0.000 description 14
• 238000001802 infusion Methods 0.000 description 14
• 239000000523 sample Substances 0.000 description 14
• 210000001519 tissue Anatomy 0.000 description 14
• 108091032973 (ribonucleotides)n+m Proteins 0.000 description 13
• 108010002386 Interleukin-3 Proteins 0.000 description 13
• 206010061902 Pancreatic neoplasm Diseases 0.000 description 13
• 239000003814 drug Substances 0.000 description 13
• 201000001441 melanoma Diseases 0.000 description 13
• 208000008443 pancreatic carcinoma Diseases 0.000 description 13
• 239000000126 substance Substances 0.000 description 13
• 102000019034 Chemokines Human genes 0.000 description 12
• 108010012236 Chemokines Proteins 0.000 description 12
• 102100039064 Interleukin-3 Human genes 0.000 description 12
• 108091054437 MHC class I family Proteins 0.000 description 12
• 230000000295 complement effect Effects 0.000 description 12
• 230000000875 corresponding effect Effects 0.000 description 12
• 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 12
• MHMNJMPURVTYEJ-UHFFFAOYSA-N fluorescein-5-isothiocyanate Chemical compound O1C(=O)C2=CC(N=C=S)=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 MHMNJMPURVTYEJ-UHFFFAOYSA-N 0.000 description 12
• 239000000463 material Substances 0.000 description 12
• 201000002528 pancreatic cancer Diseases 0.000 description 12
• 239000008194 pharmaceutical composition Substances 0.000 description 12
• 101000914484 Homo sapiens T-lymphocyte activation antigen CD80 Proteins 0.000 description 11
• 102000043129 MHC class I family Human genes 0.000 description 11
• 108091054438 MHC class II family Proteins 0.000 description 11
• 239000012980 RPMI-1640 medium Substances 0.000 description 11
• 108010039445 Stem Cell Factor Proteins 0.000 description 11
• 102100027222 T-lymphocyte activation antigen CD80 Human genes 0.000 description 11
• 241000700605 Viruses Species 0.000 description 11
• 238000012217 deletion Methods 0.000 description 11
• 230000037430 deletion Effects 0.000 description 11
• 230000003902 lesion Effects 0.000 description 11
• 208000015486 malignant pancreatic neoplasm Diseases 0.000 description 11
• 102000005962 receptors Human genes 0.000 description 11
• 108020003175 receptors Proteins 0.000 description 11
• 230000000638 stimulation Effects 0.000 description 11
• 238000006467 substitution reaction Methods 0.000 description 11
• 238000012353 t test Methods 0.000 description 11
• 102100031573 Hematopoietic progenitor cell antigen CD34 Human genes 0.000 description 10
• 101000777663 Homo sapiens Hematopoietic progenitor cell antigen CD34 Proteins 0.000 description 10
• 108010064593 Intercellular Adhesion Molecule-1 Proteins 0.000 description 10
• 102000015271 Intercellular Adhesion Molecule-1 Human genes 0.000 description 10
• 102000043131 MHC class II family Human genes 0.000 description 10
• 150000001875 compounds Chemical class 0.000 description 10
• 229940079593 drug Drugs 0.000 description 10
• 238000005516 engineering process Methods 0.000 description 10
• 230000002269 spontaneous effect Effects 0.000 description 10
• 108700028369 Alleles Proteins 0.000 description 9
• 241000282412 Homo Species 0.000 description 9
• 108010002350 Interleukin-2 Proteins 0.000 description 9
• 102000000588 Interleukin-2 Human genes 0.000 description 9
• 108010072866 Prostate-Specific Antigen Proteins 0.000 description 9
• 102000007066 Prostate-Specific Antigen Human genes 0.000 description 9
• 108091008874 T cell receptors Proteins 0.000 description 9
• 102000016266 T-Cell Antigen Receptors Human genes 0.000 description 9
• 238000007792 addition Methods 0.000 description 9
• 230000001472 cytotoxic effect Effects 0.000 description 9
• 230000006870 function Effects 0.000 description 9
• 210000000987 immune system Anatomy 0.000 description 9
• 230000001404 mediated effect Effects 0.000 description 9
• 239000000047 product Substances 0.000 description 9
• 230000010076 replication Effects 0.000 description 9
• 230000028327 secretion Effects 0.000 description 9
• 238000012546 transfer Methods 0.000 description 9
• 108010029697 CD40 Ligand Proteins 0.000 description 8
• 102100032937 CD40 ligand Human genes 0.000 description 8
• 102000014914 Carrier Proteins Human genes 0.000 description 8
• 101000599852 Homo sapiens Intercellular adhesion molecule 1 Proteins 0.000 description 8
• 101001063392 Homo sapiens Lymphocyte function-associated antigen 3 Proteins 0.000 description 8
• 102100037877 Intercellular adhesion molecule 1 Human genes 0.000 description 8
• 102000004388 Interleukin-4 Human genes 0.000 description 8
• 108090000978 Interleukin-4 Proteins 0.000 description 8
• 102100030984 Lymphocyte function-associated antigen 3 Human genes 0.000 description 8
• 102100040247 Tumor necrosis factor Human genes 0.000 description 8
• 125000000539 amino acid group Chemical group 0.000 description 8
• 201000008275 breast carcinoma Diseases 0.000 description 8
• 201000010099 disease Diseases 0.000 description 8
• 108020001507 fusion proteins Proteins 0.000 description 8
• 102000037865 fusion proteins Human genes 0.000 description 8
• 210000002540 macrophage Anatomy 0.000 description 8
• 239000013642 negative control Substances 0.000 description 8
• 210000005259 peripheral blood Anatomy 0.000 description 8
• 239000011886 peripheral blood Substances 0.000 description 8
• 230000001105 regulatory effect Effects 0.000 description 8
• 230000004044 response Effects 0.000 description 8
• UCSJYZPVAKXKNQ-HZYVHMACSA-N streptomycin Chemical compound CN[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O[C@H]1O[C@@H]1[C@](C=O)(O)[C@H](C)O[C@H]1O[C@@H]1[C@@H](NC(N)=N)[C@H](O)[C@@H](NC(N)=N)[C@H](O)[C@H]1O UCSJYZPVAKXKNQ-HZYVHMACSA-N 0.000 description 8
• 238000013518 transcription Methods 0.000 description 8
• 230000035897 transcription Effects 0.000 description 8
• 108010084313 CD58 Antigens Proteins 0.000 description 7
• 102100025475 Carcinoembryonic antigen-related cell adhesion molecule 5 Human genes 0.000 description 7
• 102000053602 DNA Human genes 0.000 description 7
• GHASVSINZRGABV-UHFFFAOYSA-N Fluorouracil Chemical compound FC1=CNC(=O)NC1=O GHASVSINZRGABV-UHFFFAOYSA-N 0.000 description 7
• 101000581981 Homo sapiens Neural cell adhesion molecule 1 Proteins 0.000 description 7
• 241000701044 Human gammaherpesvirus 4 Species 0.000 description 7
• 102100020880 Kit ligand Human genes 0.000 description 7
• 102100027347 Neural cell adhesion molecule 1 Human genes 0.000 description 7
• 102100033082 TNF receptor-associated factor 3 Human genes 0.000 description 7
• 206010046865 Vaccinia virus infection Diseases 0.000 description 7
• 230000030741 antigen processing and presentation Effects 0.000 description 7
• 239000011324 bead Substances 0.000 description 7
• 108091008324 binding proteins Proteins 0.000 description 7
• 210000000481 breast Anatomy 0.000 description 7
• 238000004422 calculation algorithm Methods 0.000 description 7
• 210000000349 chromosome Anatomy 0.000 description 7
• 239000002299 complementary DNA Substances 0.000 description 7
• 239000012228 culture supernatant Substances 0.000 description 7
• 238000002474 experimental method Methods 0.000 description 7
• 238000000338 in vitro Methods 0.000 description 7
• 210000001616 monocyte Anatomy 0.000 description 7
• 230000008488 polyadenylation Effects 0.000 description 7
• 230000009467 reduction Effects 0.000 description 7
• 239000000758 substrate Substances 0.000 description 7
• 239000006228 supernatant Substances 0.000 description 7
• 230000001225 therapeutic effect Effects 0.000 description 7
• 238000010361 transduction Methods 0.000 description 7
• 230000026683 transduction Effects 0.000 description 7
• 208000007089 vaccinia Diseases 0.000 description 7
• 201000009030 Carcinoma Diseases 0.000 description 6
• 108020004705 Codon Proteins 0.000 description 6
• 208000001333 Colorectal Neoplasms Diseases 0.000 description 6
• 102000008949 Histocompatibility Antigens Class I Human genes 0.000 description 6
• 101000850762 Homo sapiens TNF receptor-associated factor 3 Proteins 0.000 description 6
• 108010063954 Mucins Proteins 0.000 description 6
• 102000015728 Mucins Human genes 0.000 description 6
• 102000007079 Peptide Fragments Human genes 0.000 description 6
• 108010033276 Peptide Fragments Proteins 0.000 description 6
• FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 6
• 230000006044 T cell activation Effects 0.000 description 6
• 102000000852 Tumor Necrosis Factor-alpha Human genes 0.000 description 6
• 230000001464 adherent effect Effects 0.000 description 6
• 210000001185 bone marrow Anatomy 0.000 description 6
• 231100000433 cytotoxic Toxicity 0.000 description 6
• 238000001514 detection method Methods 0.000 description 6
• 238000011161 development Methods 0.000 description 6
• 230000018109 developmental process Effects 0.000 description 6
• 238000010790 dilution Methods 0.000 description 6
• 239000012895 dilution Substances 0.000 description 6
• 238000010494 dissociation reaction Methods 0.000 description 6
• 230000005593 dissociations Effects 0.000 description 6
• 239000001963 growth medium Substances 0.000 description 6
• 210000002443 helper t lymphocyte Anatomy 0.000 description 6
• 210000003958 hematopoietic stem cell Anatomy 0.000 description 6
• 238000001727 in vivo Methods 0.000 description 6
• 230000001965 increasing effect Effects 0.000 description 6
• 230000005764 inhibitory process Effects 0.000 description 6
• 239000003446 ligand Substances 0.000 description 6
• 239000003550 marker Substances 0.000 description 6
• 239000012528 membrane Substances 0.000 description 6
• 210000004379 membrane Anatomy 0.000 description 6
• 108020004999 messenger RNA Proteins 0.000 description 6
• 239000000816 peptidomimetic Substances 0.000 description 6
• 230000008569 process Effects 0.000 description 6
• 230000009257 reactivity Effects 0.000 description 6
• 238000000926 separation method Methods 0.000 description 6
• 241000894007 species Species 0.000 description 6
• 238000010186 staining Methods 0.000 description 6
• 230000004936 stimulating effect Effects 0.000 description 6
• 238000001890 transfection Methods 0.000 description 6
• 102100036846 C-C motif chemokine 21 Human genes 0.000 description 5
• 102100027207 CD27 antigen Human genes 0.000 description 5
• 108010063916 CD40 Antigens Proteins 0.000 description 5
• 241000701022 Cytomegalovirus Species 0.000 description 5
• 102000004190 Enzymes Human genes 0.000 description 5
• 108090000790 Enzymes Proteins 0.000 description 5
• 208000000666 Fowlpox Diseases 0.000 description 5
• DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 5
• 102000011786 HLA-A Antigens Human genes 0.000 description 5
• 108010075704 HLA-A Antigens Proteins 0.000 description 5
• 108010088652 Histocompatibility Antigens Class I Proteins 0.000 description 5
• 101000914511 Homo sapiens CD27 antigen Proteins 0.000 description 5
• 241000725303 Human immunodeficiency virus Species 0.000 description 5
• 108060003951 Immunoglobulin Proteins 0.000 description 5
• 241000699666 Mus <mouse, genus> Species 0.000 description 5
• 210000004369 blood Anatomy 0.000 description 5
• 239000008280 blood Substances 0.000 description 5
• 229910002091 carbon monoxide Inorganic materials 0.000 description 5
• 239000000969 carrier Substances 0.000 description 5
• 230000010261 cell growth Effects 0.000 description 5
• 238000006243 chemical reaction Methods 0.000 description 5
• LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 description 5
• 238000011124 ex vivo culture Methods 0.000 description 5
• 230000012010 growth Effects 0.000 description 5
• 102000018358 immunoglobulin Human genes 0.000 description 5
• 238000003780 insertion Methods 0.000 description 5
• 230000037431 insertion Effects 0.000 description 5
• 210000000265 leukocyte Anatomy 0.000 description 5
• 239000002502 liposome Substances 0.000 description 5
• 230000035772 mutation Effects 0.000 description 5
• 210000000056 organ Anatomy 0.000 description 5
• 239000002953 phosphate buffered saline Substances 0.000 description 5
• 230000000717 retained effect Effects 0.000 description 5
• 150000003839 salts Chemical class 0.000 description 5
• YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical group N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 4
• VVIAGPKUTFNRDU-UHFFFAOYSA-N 6S-folinic acid Natural products C1NC=2NC(N)=NC(=O)C=2N(C=O)C1CNC1=CC=C(C(=O)NC(CCC(O)=O)C(O)=O)C=C1 VVIAGPKUTFNRDU-UHFFFAOYSA-N 0.000 description 4
• 241000700663 Avipoxvirus Species 0.000 description 4
• SNKUSVNHTCUELQ-UOGODTEOSA-N CC(C)C[C@@H](C(O)=O)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](C)NC(=O)CNC(=O)[C@H](CO)NC(=O)[C@H](CC(C)C)NC(=O)[C@@H](N)CC1=CC=C(O)C=C1 Chemical compound CC(C)C[C@@H](C(O)=O)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](C)NC(=O)CNC(=O)[C@H](CO)NC(=O)[C@H](CC(C)C)NC(=O)[C@@H](N)CC1=CC=C(O)C=C1 SNKUSVNHTCUELQ-UOGODTEOSA-N 0.000 description 4
• 108050006947 CXC Chemokine Proteins 0.000 description 4
• 102000019388 CXC chemokine Human genes 0.000 description 4
• 101100328884 Caenorhabditis elegans sqt-3 gene Proteins 0.000 description 4
• 102100025473 Carcinoembryonic antigen-related cell adhesion molecule 6 Human genes 0.000 description 4
• ZHNUHDYFZUAESO-UHFFFAOYSA-N Formamide Chemical compound NC=O ZHNUHDYFZUAESO-UHFFFAOYSA-N 0.000 description 4
• 241000700662 Fowlpox virus Species 0.000 description 4
• 108010017080 Granulocyte Colony-Stimulating Factor Proteins 0.000 description 4
• 102000004269 Granulocyte Colony-Stimulating Factor Human genes 0.000 description 4
• 101000713085 Homo sapiens C-C motif chemokine 21 Proteins 0.000 description 4
• 101000914326 Homo sapiens Carcinoembryonic antigen-related cell adhesion molecule 6 Proteins 0.000 description 4
• 101001002657 Homo sapiens Interleukin-2 Proteins 0.000 description 4
• 101001133056 Homo sapiens Mucin-1 Proteins 0.000 description 4
• 102000015696 Interleukins Human genes 0.000 description 4
• 108010063738 Interleukins Proteins 0.000 description 4
• COLNVLDHVKWLRT-QMMMGPOBSA-N L-phenylalanine Chemical compound OC(=O)[C@@H](N)CC1=CC=CC=C1 COLNVLDHVKWLRT-QMMMGPOBSA-N 0.000 description 4
• 108010052285 Membrane Proteins Proteins 0.000 description 4
• 206010027476 Metastases Diseases 0.000 description 4
• 241000204031 Mycoplasma Species 0.000 description 4
• 108091005461 Nucleic proteins Proteins 0.000 description 4
• 239000012124 Opti-MEM Substances 0.000 description 4
• 102100021669 Stromal cell-derived factor 1 Human genes 0.000 description 4
• 101710088580 Stromal cell-derived factor 1 Proteins 0.000 description 4
• IQFYYKKMVGJFEH-XLPZGREQSA-N Thymidine Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](CO)[C@@H](O)C1 IQFYYKKMVGJFEH-XLPZGREQSA-N 0.000 description 4
• 241000700618 Vaccinia virus Species 0.000 description 4
• OIRDTQYFTABQOQ-KQYNXXCUSA-N adenosine Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](CO)[C@@H](O)[C@H]1O OIRDTQYFTABQOQ-KQYNXXCUSA-N 0.000 description 4
• 230000000735 allogeneic effect Effects 0.000 description 4
• 239000012298 atmosphere Substances 0.000 description 4
• 239000000872 buffer Substances 0.000 description 4
• 230000024245 cell differentiation Effects 0.000 description 4
• 230000001413 cellular effect Effects 0.000 description 4
• 239000003153 chemical reaction reagent Substances 0.000 description 4
• 238000002512 chemotherapy Methods 0.000 description 4
• CVSVTCORWBXHQV-UHFFFAOYSA-N creatine Chemical compound NC(=[NH2+])N(C)CC([O-])=O CVSVTCORWBXHQV-UHFFFAOYSA-N 0.000 description 4
• 125000000151 cysteine group Chemical group N[C@@H](CS)C(=O)* 0.000 description 4
• 230000007423 decrease Effects 0.000 description 4
• 208000035475 disorder Diseases 0.000 description 4
• VVIAGPKUTFNRDU-ABLWVSNPSA-N folinic acid Chemical compound C1NC=2NC(N)=NC(=O)C=2N(C=O)C1CNC1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 VVIAGPKUTFNRDU-ABLWVSNPSA-N 0.000 description 4
• 235000008191 folinic acid Nutrition 0.000 description 4
• 239000011672 folinic acid Substances 0.000 description 4
• 238000009472 formulation Methods 0.000 description 4
• ZDXPYRJPNDTMRX-UHFFFAOYSA-N glutamine Natural products OC(=O)C(N)CCC(N)=O ZDXPYRJPNDTMRX-UHFFFAOYSA-N 0.000 description 4
• 238000003384 imaging method Methods 0.000 description 4
• 230000003053 immunization Effects 0.000 description 4
• 238000002649 immunization Methods 0.000 description 4
• 238000010166 immunofluorescence Methods 0.000 description 4
• 229940047122 interleukins Drugs 0.000 description 4
• 238000002955 isolation Methods 0.000 description 4
• 229960001691 leucovorin Drugs 0.000 description 4
• 230000000670 limiting effect Effects 0.000 description 4
• 230000002934 lysing effect Effects 0.000 description 4
• 238000002595 magnetic resonance imaging Methods 0.000 description 4
• 239000011159 matrix material Substances 0.000 description 4
• 230000009401 metastasis Effects 0.000 description 4
• 210000000440 neutrophil Anatomy 0.000 description 4
• 239000002245 particle Substances 0.000 description 4
• 230000002265 prevention Effects 0.000 description 4
• 230000035755 proliferation Effects 0.000 description 4
• 239000011780 sodium chloride Substances 0.000 description 4
• 238000007619 statistical method Methods 0.000 description 4
• 229960005322 streptomycin Drugs 0.000 description 4
• 238000012360 testing method Methods 0.000 description 4
• 230000014616 translation Effects 0.000 description 4
• 238000002054 transplantation Methods 0.000 description 4
• 230000032258 transport Effects 0.000 description 4
• 241000701161 unidentified adenovirus Species 0.000 description 4
• 239000003981 vehicle Substances 0.000 description 4
• 241000271566 Aves Species 0.000 description 3
• 108090001008 Avidin Proteins 0.000 description 3
• 241000702421 Dependoparvovirus Species 0.000 description 3
• 229920002307 Dextran Polymers 0.000 description 3
• 101000994375 Homo sapiens Integrin alpha-4 Proteins 0.000 description 3
• 102100032818 Integrin alpha-4 Human genes 0.000 description 3
• 102000003814 Interleukin-10 Human genes 0.000 description 3
• 108090000174 Interleukin-10 Proteins 0.000 description 3
• 108010002616 Interleukin-5 Proteins 0.000 description 3
• 102000000743 Interleukin-5 Human genes 0.000 description 3
• 241000829100 Macaca mulatta polyomavirus 1 Species 0.000 description 3
• 102000018697 Membrane Proteins Human genes 0.000 description 3
• 101100335081 Mus musculus Flt3 gene Proteins 0.000 description 3
• 108091034117 Oligonucleotide Proteins 0.000 description 3
• 206010033128 Ovarian cancer Diseases 0.000 description 3
• 241000609499 Palicourea Species 0.000 description 3
• 108020004511 Recombinant DNA Proteins 0.000 description 3
• 241000714474 Rous sarcoma virus Species 0.000 description 3
• 210000000447 Th1 cell Anatomy 0.000 description 3
• 210000004241 Th2 cell Anatomy 0.000 description 3
• 230000002159 abnormal effect Effects 0.000 description 3
• 239000002253 acid Substances 0.000 description 3
• 208000009956 adenocarcinoma Diseases 0.000 description 3
• WNROFYMDJYEPJX-UHFFFAOYSA-K aluminium hydroxide Chemical compound [OH-].[OH-].[OH-].[Al+3] WNROFYMDJYEPJX-UHFFFAOYSA-K 0.000 description 3
• 230000004071 biological effect Effects 0.000 description 3
• 230000015556 catabolic process Effects 0.000 description 3
• 238000004113 cell culture Methods 0.000 description 3
• 230000036755 cellular response Effects 0.000 description 3
• 230000008859 change Effects 0.000 description 3
• 239000011248 coating agent Substances 0.000 description 3
• 238000000576 coating method Methods 0.000 description 3
• 201000010989 colorectal carcinoma Diseases 0.000 description 3
• 102000003675 cytokine receptors Human genes 0.000 description 3
• 108010057085 cytokine receptors Proteins 0.000 description 3
• 210000000805 cytoplasm Anatomy 0.000 description 3
• 230000006378 damage Effects 0.000 description 3
• 230000001419 dependent effect Effects 0.000 description 3
• 239000003085 diluting agent Substances 0.000 description 3
• 238000009826 distribution Methods 0.000 description 3
• 238000004520 electroporation Methods 0.000 description 3
• 229960002949 fluorouracil Drugs 0.000 description 3
• 238000001476 gene delivery Methods 0.000 description 3
• 210000003714 granulocyte Anatomy 0.000 description 3
• 208000037824 growth disorder Diseases 0.000 description 3
• 230000006801 homologous recombination Effects 0.000 description 3
• 238000002744 homologous recombination Methods 0.000 description 3
• 230000036039 immunity Effects 0.000 description 3
• 230000005847 immunogenicity Effects 0.000 description 3
• 230000006872 improvement Effects 0.000 description 3
• 238000011534 incubation Methods 0.000 description 3
• 239000007924 injection Substances 0.000 description 3
• 238000002347 injection Methods 0.000 description 3
• 230000010354 integration Effects 0.000 description 3
• 230000003993 interaction Effects 0.000 description 3
• 229940028885 interleukin-4 Drugs 0.000 description 3
• 208000032839 leukemia Diseases 0.000 description 3
• 230000035800 maturation Effects 0.000 description 3
• 231100000682 maximum tolerated dose Toxicity 0.000 description 3
• 210000003071 memory t lymphocyte Anatomy 0.000 description 3
• 239000004005 microsphere Substances 0.000 description 3
• 238000010369 molecular cloning Methods 0.000 description 3
• 210000005087 mononuclear cell Anatomy 0.000 description 3
• 230000037361 pathway Effects 0.000 description 3
• 235000020030 perry Nutrition 0.000 description 3
• 239000000546 pharmaceutical excipient Substances 0.000 description 3
• 239000013641 positive control Substances 0.000 description 3
• 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 3
• 230000002062 proliferating effect Effects 0.000 description 3
• 230000000069 prophylactic effect Effects 0.000 description 3
• 238000001959 radiotherapy Methods 0.000 description 3
• 230000000284 resting effect Effects 0.000 description 3
• 238000012216 screening Methods 0.000 description 3
• 210000002966 serum Anatomy 0.000 description 3
• 239000007787 solid Substances 0.000 description 3
• 230000009870 specific binding Effects 0.000 description 3
• 238000010561 standard procedure Methods 0.000 description 3
• 238000010254 subcutaneous injection Methods 0.000 description 3
• 239000007929 subcutaneous injection Substances 0.000 description 3
• 229940124597 therapeutic agent Drugs 0.000 description 3
• 238000002560 therapeutic procedure Methods 0.000 description 3
• 210000001541 thymus gland Anatomy 0.000 description 3
• 238000004448 titration Methods 0.000 description 3
• 230000002463 transducing effect Effects 0.000 description 3
• 238000013519 translation Methods 0.000 description 3
• 238000011144 upstream manufacturing Methods 0.000 description 3
• OZFAFGSSMRRTDW-UHFFFAOYSA-N (2,4-dichlorophenyl) benzenesulfonate Chemical compound ClC1=CC(Cl)=CC=C1OS(=O)(=O)C1=CC=CC=C1 OZFAFGSSMRRTDW-UHFFFAOYSA-N 0.000 description 2
• WEYNBWVKOYCCQT-UHFFFAOYSA-N 1-(3-chloro-4-methylphenyl)-3-{2-[({5-[(dimethylamino)methyl]-2-furyl}methyl)thio]ethyl}urea Chemical compound O1C(CN(C)C)=CC=C1CSCCNC(=O)NC1=CC=C(C)C(Cl)=C1 WEYNBWVKOYCCQT-UHFFFAOYSA-N 0.000 description 2
• 102000007469 Actins Human genes 0.000 description 2
• 108010085238 Actins Proteins 0.000 description 2
• 101710137115 Adenylyl cyclase-associated protein 1 Proteins 0.000 description 2
• IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
• 102100023995 Beta-nerve growth factor Human genes 0.000 description 2
• 108091003079 Bovine Serum Albumin Proteins 0.000 description 2
• 102100036842 C-C motif chemokine 19 Human genes 0.000 description 2
• 102100036848 C-C motif chemokine 20 Human genes 0.000 description 2
• 102100035793 CD83 antigen Human genes 0.000 description 2
• 241000283707 Capra Species 0.000 description 2
• 102000016289 Cell Adhesion Molecules Human genes 0.000 description 2
• 108010067225 Cell Adhesion Molecules Proteins 0.000 description 2
• 206010057248 Cell death Diseases 0.000 description 2
• 102000007644 Colony-Stimulating Factors Human genes 0.000 description 2
• 108010071942 Colony-Stimulating Factors Proteins 0.000 description 2
• FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 description 2
• 150000008574 D-amino acids Chemical class 0.000 description 2
• IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 2
• 239000012591 Dulbecco’s Phosphate Buffered Saline Substances 0.000 description 2
• 238000008157 ELISA kit Methods 0.000 description 2
• 101150029707 ERBB2 gene Proteins 0.000 description 2
• 102100025137 Early activation antigen CD69 Human genes 0.000 description 2
• 102100023688 Eotaxin Human genes 0.000 description 2
• 101710139422 Eotaxin Proteins 0.000 description 2
• 229920001917 Ficoll Polymers 0.000 description 2
• 101710113436 GTPase KRas Proteins 0.000 description 2
• 206010017993 Gastrointestinal neoplasms Diseases 0.000 description 2
• 229930182566 Gentamicin Natural products 0.000 description 2
• CEAZRRDELHUEMR-URQXQFDESA-N Gentamicin Chemical compound O1[C@H](C(C)NC)CC[C@@H](N)[C@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](NC)[C@@](C)(O)CO2)O)[C@H](N)C[C@@H]1N CEAZRRDELHUEMR-URQXQFDESA-N 0.000 description 2
• WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
• 239000004471 Glycine Substances 0.000 description 2
• ZRALSGWEFCBTJO-UHFFFAOYSA-N Guanidine Chemical compound NC(N)=N ZRALSGWEFCBTJO-UHFFFAOYSA-N 0.000 description 2
• NYHBQMYGNKIUIF-UUOKFMHZSA-N Guanosine Chemical compound C1=NC=2C(=O)NC(N)=NC=2N1[C@@H]1O[C@H](CO)[C@@H](O)[C@H]1O NYHBQMYGNKIUIF-UUOKFMHZSA-N 0.000 description 2
• 108010086377 HLA-A3 Antigen Proteins 0.000 description 2
• 102000015789 HLA-DP Antigens Human genes 0.000 description 2
• 108010010378 HLA-DP Antigens Proteins 0.000 description 2
• 102000006354 HLA-DR Antigens Human genes 0.000 description 2
• 108010058597 HLA-DR Antigens Proteins 0.000 description 2
• 102000001554 Hemoglobins Human genes 0.000 description 2
• 108010054147 Hemoglobins Proteins 0.000 description 2
• 101000713099 Homo sapiens C-C motif chemokine 20 Proteins 0.000 description 2
• 101000934374 Homo sapiens Early activation antigen CD69 Proteins 0.000 description 2
• 101000746373 Homo sapiens Granulocyte-macrophage colony-stimulating factor Proteins 0.000 description 2
• 101001057504 Homo sapiens Interferon-stimulated gene 20 kDa protein Proteins 0.000 description 2
• 101001055144 Homo sapiens Interleukin-2 receptor subunit alpha Proteins 0.000 description 2
• 101001002709 Homo sapiens Interleukin-4 Proteins 0.000 description 2
• 101000947178 Homo sapiens Platelet basic protein Proteins 0.000 description 2
• 101000851376 Homo sapiens Tumor necrosis factor receptor superfamily member 8 Proteins 0.000 description 2
• 108010002352 Interleukin-1 Proteins 0.000 description 2
• 102000000589 Interleukin-1 Human genes 0.000 description 2
• 102100026878 Interleukin-2 receptor subunit alpha Human genes 0.000 description 2
• AGPKZVBTJJNPAG-WHFBIAKZSA-N L-isoleucine Chemical compound CC[C@H](C)[C@H](N)C(O)=O AGPKZVBTJJNPAG-WHFBIAKZSA-N 0.000 description 2
• ROHFNLRQFUQHCH-YFKPBYRVSA-N L-leucine Chemical compound CC(C)C[C@H](N)C(O)=O ROHFNLRQFUQHCH-YFKPBYRVSA-N 0.000 description 2
• 229930195725 Mannitol Natural products 0.000 description 2
• 241001465754 Metazoa Species 0.000 description 2
• 208000034578 Multiple myelomas Diseases 0.000 description 2
• 241001529936 Murinae Species 0.000 description 2
• 102000003505 Myosin Human genes 0.000 description 2
• 108060008487 Myosin Proteins 0.000 description 2
• JDHILDINMRGULE-LURJTMIESA-N N(pros)-methyl-L-histidine Chemical compound CN1C=NC=C1C[C@H](N)C(O)=O JDHILDINMRGULE-LURJTMIESA-N 0.000 description 2
• 108091061960 Naked DNA Proteins 0.000 description 2
• 108010025020 Nerve Growth Factor Proteins 0.000 description 2
• 238000000636 Northern blotting Methods 0.000 description 2
• 206010061535 Ovarian neoplasm Diseases 0.000 description 2
• 229930182555 Penicillin Natural products 0.000 description 2
• JGSARLDLIJGVTE-MBNYWOFBSA-N Penicillin G Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)CC1=CC=CC=C1 JGSARLDLIJGVTE-MBNYWOFBSA-N 0.000 description 2
• 206010035226 Plasma cell myeloma Diseases 0.000 description 2
• 102100036154 Platelet basic protein Human genes 0.000 description 2
• 241000288906 Primates Species 0.000 description 2
• 241000700159 Rattus Species 0.000 description 2
• 108091081062 Repeated sequence (DNA) Proteins 0.000 description 2
• 238000012300 Sequence Analysis Methods 0.000 description 2
• 108010071390 Serum Albumin Proteins 0.000 description 2
• 102000007562 Serum Albumin Human genes 0.000 description 2
• CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 2
• 229930006000 Sucrose Natural products 0.000 description 2
• 230000006052 T cell proliferation Effects 0.000 description 2
• 102100031988 Tumor necrosis factor ligand superfamily member 6 Human genes 0.000 description 2
• 108050002568 Tumor necrosis factor ligand superfamily member 6 Proteins 0.000 description 2
• 102100036857 Tumor necrosis factor receptor superfamily member 8 Human genes 0.000 description 2
• DRTQHJPVMGBUCF-XVFCMESISA-N Uridine Chemical compound O[C@@H]1[C@H](O)[C@@H](CO)O[C@H]1N1C(=O)NC(=O)C=C1 DRTQHJPVMGBUCF-XVFCMESISA-N 0.000 description 2
• 240000008042 Zea mays Species 0.000 description 2
• 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 2
• 235000002017 Zea mays subsp mays Nutrition 0.000 description 2
• 150000007513 acids Chemical class 0.000 description 2
• 230000002411 adverse Effects 0.000 description 2
• 238000010171 animal model Methods 0.000 description 2
• 230000000259 anti-tumor effect Effects 0.000 description 2
• 230000006023 anti-tumor response Effects 0.000 description 2
• 238000002617 apheresis Methods 0.000 description 2
• 238000013459 approach Methods 0.000 description 2
• 230000001580 bacterial effect Effects 0.000 description 2
• WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 2
• 229960002685 biotin Drugs 0.000 description 2
• 235000020958 biotin Nutrition 0.000 description 2
• 239000011616 biotin Substances 0.000 description 2
• 201000008274 breast adenocarcinoma Diseases 0.000 description 2
• KQNZDYYTLMIZCT-KQPMLPITSA-N brefeldin A Chemical compound O[C@@H]1\C=C\C(=O)O[C@@H](C)CCC\C=C\[C@@H]2C[C@H](O)C[C@H]21 KQNZDYYTLMIZCT-KQPMLPITSA-N 0.000 description 2
• JUMGSHROWPPKFX-UHFFFAOYSA-N brefeldin-A Natural products CC1CCCC=CC2(C)CC(O)CC2(C)C(O)C=CC(=O)O1 JUMGSHROWPPKFX-UHFFFAOYSA-N 0.000 description 2
• 239000007975 buffered saline Substances 0.000 description 2
• 210000004899 c-terminal region Anatomy 0.000 description 2
• 239000001506 calcium phosphate Substances 0.000 description 2
• 229910000389 calcium phosphate Inorganic materials 0.000 description 2
• 235000011010 calcium phosphates Nutrition 0.000 description 2
• 230000030833 cell death Effects 0.000 description 2
• 230000005859 cell recognition Effects 0.000 description 2
• 238000012512 characterization method Methods 0.000 description 2
• 230000035605 chemotaxis Effects 0.000 description 2
• HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 2
• 230000002759 chromosomal effect Effects 0.000 description 2
• 238000010367 cloning Methods 0.000 description 2
• 229940047120 colony stimulating factors Drugs 0.000 description 2
• 238000004590 computer program Methods 0.000 description 2
• 238000010276 construction Methods 0.000 description 2
• 238000007796 conventional method Methods 0.000 description 2
• 235000005822 corn Nutrition 0.000 description 2
• 229960003624 creatine Drugs 0.000 description 2
• 239000006046 creatine Substances 0.000 description 2
• 210000004748 cultured cell Anatomy 0.000 description 2
• 238000012258 culturing Methods 0.000 description 2
• XUJNEKJLAYXESH-UHFFFAOYSA-N cysteine Natural products SCC(N)C(O)=O XUJNEKJLAYXESH-UHFFFAOYSA-N 0.000 description 2
• 235000018417 cysteine Nutrition 0.000 description 2
• OPTASPLRGRRNAP-UHFFFAOYSA-N cytosine Chemical compound NC=1C=CNC(=O)N=1 OPTASPLRGRRNAP-UHFFFAOYSA-N 0.000 description 2
• 230000001086 cytosolic effect Effects 0.000 description 2
• 231100000599 cytotoxic agent Toxicity 0.000 description 2
• 230000003013 cytotoxicity Effects 0.000 description 2
• 231100000135 cytotoxicity Toxicity 0.000 description 2
• 230000003247 decreasing effect Effects 0.000 description 2
• 238000006731 degradation reaction Methods 0.000 description 2
• 238000003745 diagnosis Methods 0.000 description 2
• 238000002405 diagnostic procedure Methods 0.000 description 2
• 210000000981 epithelium Anatomy 0.000 description 2
• 238000011156 evaluation Methods 0.000 description 2
• 230000005284 excitation Effects 0.000 description 2
• 208000021045 exocrine pancreatic carcinoma Diseases 0.000 description 2
• 239000012091 fetal bovine serum Substances 0.000 description 2
• 239000007850 fluorescent dye Substances 0.000 description 2
• 238000013467 fragmentation Methods 0.000 description 2
• 238000006062 fragmentation reaction Methods 0.000 description 2
• 230000005714 functional activity Effects 0.000 description 2
• 238000002825 functional assay Methods 0.000 description 2
• 230000004927 fusion Effects 0.000 description 2
• 239000000499 gel Substances 0.000 description 2
• 238000012239 gene modification Methods 0.000 description 2
• 102000054766 genetic haplotypes Human genes 0.000 description 2
• 230000005017 genetic modification Effects 0.000 description 2
• 235000013617 genetically modified food Nutrition 0.000 description 2
• 239000008103 glucose Substances 0.000 description 2
• RWSXRVCMGQZWBV-WDSKDSINSA-N glutathione Chemical compound OC(=O)[C@@H](N)CCC(=O)N[C@@H](CS)C(=O)NCC(O)=O RWSXRVCMGQZWBV-WDSKDSINSA-N 0.000 description 2
• 239000003102 growth factor Substances 0.000 description 2
• 208000014829 head and neck neoplasm Diseases 0.000 description 2
• 102000046157 human CSF2 Human genes 0.000 description 2
• 102000055229 human IL4 Human genes 0.000 description 2
• 230000004727 humoral immunity Effects 0.000 description 2
• 210000004408 hybridoma Anatomy 0.000 description 2
• JYGXADMDTFJGBT-VWUMJDOOSA-N hydrocortisone Chemical compound O=C1CC[C@]2(C)[C@H]3[C@@H](O)C[C@](C)([C@@](CC4)(O)C(=O)CO)[C@@H]4[C@@H]3CCC2=C1 JYGXADMDTFJGBT-VWUMJDOOSA-N 0.000 description 2
• 238000003018 immunoassay Methods 0.000 description 2
• 230000016784 immunoglobulin production Effects 0.000 description 2
• 238000009169 immunotherapy Methods 0.000 description 2
• 239000007943 implant Substances 0.000 description 2
• 230000001976 improved effect Effects 0.000 description 2
• 229910052738 indium Inorganic materials 0.000 description 2
• APFVFJFRJDLVQX-UHFFFAOYSA-N indium atom Chemical compound [In] APFVFJFRJDLVQX-UHFFFAOYSA-N 0.000 description 2
• 230000006698 induction Effects 0.000 description 2
• 230000028709 inflammatory response Effects 0.000 description 2
• 230000000977 initiatory effect Effects 0.000 description 2
• 102000006495 integrins Human genes 0.000 description 2
• 108010044426 integrins Proteins 0.000 description 2
• 238000007912 intraperitoneal administration Methods 0.000 description 2
• 238000001990 intravenous administration Methods 0.000 description 2
• 238000002372 labelling Methods 0.000 description 2
• 150000002632 lipids Chemical class 0.000 description 2
• 210000004185 liver Anatomy 0.000 description 2
• 210000004072 lung Anatomy 0.000 description 2
• 210000002751 lymph Anatomy 0.000 description 2
• HQKMJHAJHXVSDF-UHFFFAOYSA-L magnesium stearate Chemical compound [Mg+2].CCCCCCCCCCCCCCCCCC([O-])=O.CCCCCCCCCCCCCCCCCC([O-])=O HQKMJHAJHXVSDF-UHFFFAOYSA-L 0.000 description 2
• 238000012423 maintenance Methods 0.000 description 2
• 210000004962 mammalian cell Anatomy 0.000 description 2
• 239000000594 mannitol Substances 0.000 description 2
• 235000010355 mannitol Nutrition 0.000 description 2
• 238000005259 measurement Methods 0.000 description 2
• 238000000520 microinjection Methods 0.000 description 2
• 238000013508 migration Methods 0.000 description 2
• 230000005012 migration Effects 0.000 description 2
• 239000002480 mineral oil Substances 0.000 description 2
• 235000010446 mineral oil Nutrition 0.000 description 2
• ZAHQPTJLOCWVPG-UHFFFAOYSA-N mitoxantrone dihydrochloride Chemical compound Cl.Cl.O=C1C2=C(O)C=CC(O)=C2C(=O)C2=C1C(NCCNCCO)=CC=C2NCCNCCO ZAHQPTJLOCWVPG-UHFFFAOYSA-N 0.000 description 2
• 210000003205 muscle Anatomy 0.000 description 2
• 230000000869 mutational effect Effects 0.000 description 2
• 230000001613 neoplastic effect Effects 0.000 description 2
• 229940053128 nerve growth factor Drugs 0.000 description 2
• 230000007935 neutral effect Effects 0.000 description 2
• 229960003540 oxyquinoline Drugs 0.000 description 2
• 238000007427 paired t-test Methods 0.000 description 2
• 230000007170 pathology Effects 0.000 description 2
• 229940049954 penicillin Drugs 0.000 description 2
• 229940023041 peptide vaccine Drugs 0.000 description 2
• LUYQYZLEHLTPBH-UHFFFAOYSA-N perfluorobutanesulfonyl fluoride Chemical compound FC(F)(F)C(F)(F)C(F)(F)C(F)(F)S(F)(=O)=O LUYQYZLEHLTPBH-UHFFFAOYSA-N 0.000 description 2
• 210000005105 peripheral blood lymphocyte Anatomy 0.000 description 2
• 239000012071 phase Substances 0.000 description 2
• 239000011148 porous material Substances 0.000 description 2
• 230000003389 potentiating effect Effects 0.000 description 2
• 239000002243 precursor Substances 0.000 description 2
• 238000002360 preparation method Methods 0.000 description 2
• 238000012545 processing Methods 0.000 description 2
• MCJGNVYPOGVAJF-UHFFFAOYSA-N quinolin-8-ol Chemical compound C1=CN=C2C(O)=CC=CC2=C1 MCJGNVYPOGVAJF-UHFFFAOYSA-N 0.000 description 2
• 230000002829 reductive effect Effects 0.000 description 2
• 238000003757 reverse transcription PCR Methods 0.000 description 2
• 238000012552 review Methods 0.000 description 2
• 102200094548 rs587777400 Human genes 0.000 description 2
• 108010038379 sargramostim Proteins 0.000 description 2
• 230000035945 sensitivity Effects 0.000 description 2
• 210000003491 skin Anatomy 0.000 description 2
• 239000000243 solution Substances 0.000 description 2
• 239000005720 sucrose Substances 0.000 description 2
• 239000013589 supplement Substances 0.000 description 2
• 238000013268 sustained release Methods 0.000 description 2
• 239000012730 sustained-release form Substances 0.000 description 2
• 208000024891 symptom Diseases 0.000 description 2
• 230000008685 targeting Effects 0.000 description 2
• 230000009466 transformation Effects 0.000 description 2
• QORWJWZARLRLPR-UHFFFAOYSA-H tricalcium bis(phosphate) Chemical compound [Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O QORWJWZARLRLPR-UHFFFAOYSA-H 0.000 description 2
• GPRLSGONYQIRFK-MNYXATJNSA-N triton Chemical compound [3H+] GPRLSGONYQIRFK-MNYXATJNSA-N 0.000 description 2
• 230000004614 tumor growth Effects 0.000 description 2
• 238000001262 western blot Methods 0.000 description 2
• DGVVWUTYPXICAM-UHFFFAOYSA-N β‐Mercaptoethanol Chemical compound OCCS DGVVWUTYPXICAM-UHFFFAOYSA-N 0.000 description 2
• DIGQNXIGRZPYDK-WKSCXVIASA-N (2R)-6-amino-2-[[2-[[(2S)-2-[[2-[[(2R)-2-[[(2S)-2-[[(2R,3S)-2-[[2-[[(2S)-2-[[2-[[(2S)-2-[[(2S)-2-[[(2R)-2-[[(2S,3S)-2-[[(2R)-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-[[2-[[(2S)-2-[[(2R)-2-[[2-[[2-[[2-[(2-amino-1-hydroxyethylidene)amino]-3-carboxy-1-hydroxypropylidene]amino]-1-hydroxy-3-sulfanylpropylidene]amino]-1-hydroxyethylidene]amino]-1-hydroxy-3-sulfanylpropylidene]amino]-1,3-dihydroxypropylidene]amino]-1-hydroxyethylidene]amino]-1-hydroxypropylidene]amino]-1,3-dihydroxypropylidene]amino]-1,3-dihydroxypropylidene]amino]-1-hydroxy-3-sulfanylpropylidene]amino]-1,3-dihydroxybutylidene]amino]-1-hydroxy-3-sulfanylpropylidene]amino]-1-hydroxypropylidene]amino]-1,3-dihydroxypropylidene]amino]-1-hydroxyethylidene]amino]-1,5-dihydroxy-5-iminopentylidene]amino]-1-hydroxy-3-sulfanylpropylidene]amino]-1,3-dihydroxybutylidene]amino]-1-hydroxy-3-sulfanylpropylidene]amino]-1,3-dihydroxypropylidene]amino]-1-hydroxyethylidene]amino]-1-hydroxy-3-sulfanylpropylidene]amino]-1-hydroxyethylidene]amino]hexanoic acid Chemical compound C[C@@H]([C@@H](C(=N[C@@H](CS)C(=N[C@@H](C)C(=N[C@@H](CO)C(=NCC(=N[C@@H](CCC(=N)O)C(=NC(CS)C(=N[C@H]([C@H](C)O)C(=N[C@H](CS)C(=N[C@H](CO)C(=NCC(=N[C@H](CS)C(=NCC(=N[C@H](CCCCN)C(=O)O)O)O)O)O)O)O)O)O)O)O)O)O)O)N=C([C@H](CS)N=C([C@H](CO)N=C([C@H](CO)N=C([C@H](C)N=C(CN=C([C@H](CO)N=C([C@H](CS)N=C(CN=C(C(CS)N=C(C(CC(=O)O)N=C(CN)O)O)O)O)O)O)O)O)O)O)O)O DIGQNXIGRZPYDK-WKSCXVIASA-N 0.000 description 1
• ASWBNKHCZGQVJV-UHFFFAOYSA-N (3-hexadecanoyloxy-2-hydroxypropyl) 2-(trimethylazaniumyl)ethyl phosphate Chemical compound CCCCCCCCCCCCCCCC(=O)OCC(O)COP([O-])(=O)OCC[N+](C)(C)C ASWBNKHCZGQVJV-UHFFFAOYSA-N 0.000 description 1
• 102000040650 (ribonucleotides)n+m Human genes 0.000 description 1
• UKAUYVFTDYCKQA-UHFFFAOYSA-N -2-Amino-4-hydroxybutanoic acid Natural products OC(=O)C(N)CCO UKAUYVFTDYCKQA-UHFFFAOYSA-N 0.000 description 1
• LUTLAXLNPLZCOF-UHFFFAOYSA-N 1-Methylhistidine Natural products OC(=O)C(N)(C)CC1=NC=CN1 LUTLAXLNPLZCOF-UHFFFAOYSA-N 0.000 description 1
• IQFYYKKMVGJFEH-OFKYTIFKSA-N 1-[(2r,4s,5r)-4-hydroxy-5-(tritiooxymethyl)oxolan-2-yl]-5-methylpyrimidine-2,4-dione Chemical compound C1[C@H](O)[C@@H](CO[3H])O[C@H]1N1C(=O)NC(=O)C(C)=C1 IQFYYKKMVGJFEH-OFKYTIFKSA-N 0.000 description 1
• UHDGCWIWMRVCDJ-UHFFFAOYSA-N 1-beta-D-Xylofuranosyl-NH-Cytosine Natural products O=C1N=C(N)C=CN1C1C(O)C(O)C(CO)O1 UHDGCWIWMRVCDJ-UHFFFAOYSA-N 0.000 description 1
• YKBGVTZYEHREMT-KVQBGUIXSA-N 2'-deoxyguanosine Chemical compound C1=NC=2C(=O)NC(N)=NC=2N1[C@H]1C[C@H](O)[C@@H](CO)O1 YKBGVTZYEHREMT-KVQBGUIXSA-N 0.000 description 1
• CKTSBUTUHBMZGZ-SHYZEUOFSA-N 2'‐deoxycytidine Chemical compound O=C1N=C(N)C=CN1[C@@H]1O[C@H](CO)[C@@H](O)C1 CKTSBUTUHBMZGZ-SHYZEUOFSA-N 0.000 description 1
• UFBJCMHMOXMLKC-UHFFFAOYSA-N 2,4-dinitrophenol Chemical compound OC1=CC=C([N+]([O-])=O)C=C1[N+]([O-])=O UFBJCMHMOXMLKC-UHFFFAOYSA-N 0.000 description 1
• JKMHFZQWWAIEOD-UHFFFAOYSA-N 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid Chemical compound OCC[NH+]1CCN(CCS([O-])(=O)=O)CC1 JKMHFZQWWAIEOD-UHFFFAOYSA-N 0.000 description 1
• BFSVOASYOCHEOV-UHFFFAOYSA-N 2-diethylaminoethanol Chemical compound CCN(CC)CCO BFSVOASYOCHEOV-UHFFFAOYSA-N 0.000 description 1
• BRMWTNUJHUMWMS-UHFFFAOYSA-N 3-Methylhistidine Natural products CN1C=NC(CC(N)C(O)=O)=C1 BRMWTNUJHUMWMS-UHFFFAOYSA-N 0.000 description 1
• RANONBLIHMVXAJ-UHFFFAOYSA-N 4-hydroxycyclophosphamide Chemical compound OC1CCOP(=O)(N(CCCl)CCCl)N1 RANONBLIHMVXAJ-UHFFFAOYSA-N 0.000 description 1
• LDCYZAJDBXYCGN-VIFPVBQESA-N 5-hydroxy-L-tryptophan Chemical compound C1=C(O)C=C2C(C[C@H](N)C(O)=O)=CNC2=C1 LDCYZAJDBXYCGN-VIFPVBQESA-N 0.000 description 1
• 229940000681 5-hydroxytryptophan Drugs 0.000 description 1
• 102100031585 ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1 Human genes 0.000 description 1
• 208000030507 AIDS Diseases 0.000 description 1
• GUBGYTABKSRVRQ-XLOQQCSPSA-N Alpha-Lactose Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)O[C@H](O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-XLOQQCSPSA-N 0.000 description 1
• 102000006306 Antigen Receptors Human genes 0.000 description 1
• 108010083359 Antigen Receptors Proteins 0.000 description 1
• 241000713826 Avian leukosis virus Species 0.000 description 1
• 208000028564 B-cell non-Hodgkin lymphoma Diseases 0.000 description 1
• 230000003844 B-cell-activation Effects 0.000 description 1
• 102100022005 B-lymphocyte antigen CD20 Human genes 0.000 description 1
• DWRXFEITVBNRMK-UHFFFAOYSA-N Beta-D-1-Arabinofuranosylthymine Natural products O=C1NC(=O)C(C)=CN1C1C(O)C(O)C(CO)O1 DWRXFEITVBNRMK-UHFFFAOYSA-N 0.000 description 1
• FERIUCNNQQJTOY-UHFFFAOYSA-M Butyrate Chemical compound CCCC([O-])=O FERIUCNNQQJTOY-UHFFFAOYSA-M 0.000 description 1
• FERIUCNNQQJTOY-UHFFFAOYSA-N Butyric acid Natural products CCCC(O)=O FERIUCNNQQJTOY-UHFFFAOYSA-N 0.000 description 1
• 108050005711 C Chemokine Proteins 0.000 description 1
• 102000017483 C chemokine Human genes 0.000 description 1
• 102100036301 C-C chemokine receptor type 7 Human genes 0.000 description 1
• 102100025074 C-C chemokine receptor-like 2 Human genes 0.000 description 1
• 102100036841 C-C motif chemokine 1 Human genes 0.000 description 1
• 101710155835 C-C motif chemokine 1 Proteins 0.000 description 1
• 102100023705 C-C motif chemokine 14 Human genes 0.000 description 1
• 101710112622 C-C motif chemokine 19 Proteins 0.000 description 1
• 102100021943 C-C motif chemokine 2 Human genes 0.000 description 1
• 101710155857 C-C motif chemokine 2 Proteins 0.000 description 1
• 102100032367 C-C motif chemokine 5 Human genes 0.000 description 1
• 102100036150 C-X-C motif chemokine 5 Human genes 0.000 description 1
• 102100036153 C-X-C motif chemokine 6 Human genes 0.000 description 1
• 101710085504 C-X-C motif chemokine 6 Proteins 0.000 description 1
• 239000002126 C01EB10 - Adenosine Substances 0.000 description 1
• 108010040471 CC Chemokines Proteins 0.000 description 1
• 102000001902 CC Chemokines Human genes 0.000 description 1
• 102100025221 CD70 antigen Human genes 0.000 description 1
• 210000001266 CD8-positive T-lymphocyte Anatomy 0.000 description 1
• 210000001239 CD8-positive, alpha-beta cytotoxic T lymphocyte Anatomy 0.000 description 1
• 108010052382 CD83 antigen Proteins 0.000 description 1
• 101150045267 CEA gene Proteins 0.000 description 1
• GAGWJHPBXLXJQN-UORFTKCHSA-N Capecitabine Chemical compound C1=C(F)C(NC(=O)OCCCCC)=NC(=O)N1[C@H]1[C@H](O)[C@H](O)[C@@H](C)O1 GAGWJHPBXLXJQN-UORFTKCHSA-N 0.000 description 1
• 101710132601 Capsid protein Proteins 0.000 description 1
• 102000011632 Caseins Human genes 0.000 description 1
• 108010076119 Caseins Proteins 0.000 description 1
• 108010082161 Chemokine CCL19 Proteins 0.000 description 1
• 102000003805 Chemokine CCL19 Human genes 0.000 description 1
• 108010083702 Chemokine CCL21 Proteins 0.000 description 1
• 108010055166 Chemokine CCL5 Proteins 0.000 description 1
• 102000009410 Chemokine receptor Human genes 0.000 description 1
• 108050000299 Chemokine receptor Proteins 0.000 description 1
• 108020004638 Circular DNA Proteins 0.000 description 1
• 208000035473 Communicable disease Diseases 0.000 description 1
• 241000557626 Corvus corax Species 0.000 description 1
• 101150073133 Cpt1a gene Proteins 0.000 description 1
• 241000699800 Cricetinae Species 0.000 description 1
• MIKUYHXYGGJMLM-GIMIYPNGSA-N Crotonoside Natural products C1=NC2=C(N)NC(=O)N=C2N1[C@H]1O[C@@H](CO)[C@H](O)[C@@H]1O MIKUYHXYGGJMLM-GIMIYPNGSA-N 0.000 description 1
• UHDGCWIWMRVCDJ-PSQAKQOGSA-N Cytidine Natural products O=C1N=C(N)C=CN1[C@@H]1[C@@H](O)[C@@H](O)[C@H](CO)O1 UHDGCWIWMRVCDJ-PSQAKQOGSA-N 0.000 description 1
• NYHBQMYGNKIUIF-UHFFFAOYSA-N D-guanosine Natural products C1=2NC(N)=NC(=O)C=2N=CN1C1OC(CO)C(O)C1O NYHBQMYGNKIUIF-UHFFFAOYSA-N 0.000 description 1
• WQZGKKKJIJFFOK-QTVWNMPRSA-N D-mannopyranose Chemical compound OC[C@H]1OC(O)[C@@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-QTVWNMPRSA-N 0.000 description 1
• 230000004543 DNA replication Effects 0.000 description 1
• CKTSBUTUHBMZGZ-UHFFFAOYSA-N Deoxycytidine Natural products O=C1N=C(N)C=CN1C1OC(CO)C(O)C1 CKTSBUTUHBMZGZ-UHFFFAOYSA-N 0.000 description 1
• 102100025012 Dipeptidyl peptidase 4 Human genes 0.000 description 1
• 239000006144 Dulbecco’s modified Eagle's medium Substances 0.000 description 1
• 108010031111 EBV-encoded nuclear antigen 1 Proteins 0.000 description 1
• KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
• 238000002965 ELISA Methods 0.000 description 1
• 241000196324 Embryophyta Species 0.000 description 1
• LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
• 108700024394 Exon Proteins 0.000 description 1
• 229910052688 Gadolinium Inorganic materials 0.000 description 1
• 101710115997 Gamma-tubulin complex component 2 Proteins 0.000 description 1
• 206010061968 Gastric neoplasm Diseases 0.000 description 1
• 101710087079 Gene 49 protein Proteins 0.000 description 1
• 108700028146 Genetic Enhancer Elements Proteins 0.000 description 1
• 108010024636 Glutathione Proteins 0.000 description 1
• 102000003886 Glycoproteins Human genes 0.000 description 1
• 108090000288 Glycoproteins Proteins 0.000 description 1
• 102000001398 Granzyme Human genes 0.000 description 1
• 108060005986 Granzyme Proteins 0.000 description 1
• 102100034221 Growth-regulated alpha protein Human genes 0.000 description 1
• 241000696272 Gull adenovirus Species 0.000 description 1
• 239000007995 HEPES buffer Substances 0.000 description 1
• 102100031547 HLA class II histocompatibility antigen, DO alpha chain Human genes 0.000 description 1
• 239000012981 Hank's balanced salt solution Substances 0.000 description 1
• 208000002250 Hematologic Neoplasms Diseases 0.000 description 1
• 208000009889 Herpes Simplex Diseases 0.000 description 1
• 102000018713 Histocompatibility Antigens Class II Human genes 0.000 description 1
• 208000017604 Hodgkin disease Diseases 0.000 description 1
• 208000021519 Hodgkin lymphoma Diseases 0.000 description 1
• 208000010747 Hodgkins lymphoma Diseases 0.000 description 1
• 101000777636 Homo sapiens ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1 Proteins 0.000 description 1
• 101000897405 Homo sapiens B-lymphocyte antigen CD20 Proteins 0.000 description 1
• 101000716068 Homo sapiens C-C chemokine receptor type 6 Proteins 0.000 description 1
• 101000716065 Homo sapiens C-C chemokine receptor type 7 Proteins 0.000 description 1
• 101000978381 Homo sapiens C-C motif chemokine 14 Proteins 0.000 description 1
• 101000713106 Homo sapiens C-C motif chemokine 19 Proteins 0.000 description 1
• 101000947186 Homo sapiens C-X-C motif chemokine 5 Proteins 0.000 description 1
• 101100099884 Homo sapiens CD40 gene Proteins 0.000 description 1
• 101000934356 Homo sapiens CD70 antigen Proteins 0.000 description 1
• 101000946856 Homo sapiens CD83 antigen Proteins 0.000 description 1
• 101000908391 Homo sapiens Dipeptidyl peptidase 4 Proteins 0.000 description 1
• 101001069921 Homo sapiens Growth-regulated alpha protein Proteins 0.000 description 1
• 101000866278 Homo sapiens HLA class II histocompatibility antigen, DO alpha chain Proteins 0.000 description 1
• 101100452011 Homo sapiens ICAM1 gene Proteins 0.000 description 1
• 101001033279 Homo sapiens Interleukin-3 Proteins 0.000 description 1
• 101000984190 Homo sapiens Leukocyte immunoglobulin-like receptor subfamily B member 1 Proteins 0.000 description 1
• 101000984192 Homo sapiens Leukocyte immunoglobulin-like receptor subfamily B member 3 Proteins 0.000 description 1
• 101100346929 Homo sapiens MUC1 gene Proteins 0.000 description 1
• 101000973997 Homo sapiens Nucleosome assembly protein 1-like 4 Proteins 0.000 description 1
• 101001012157 Homo sapiens Receptor tyrosine-protein kinase erbB-2 Proteins 0.000 description 1
• 101000914514 Homo sapiens T-cell-specific surface glycoprotein CD28 Proteins 0.000 description 1
• 101000835093 Homo sapiens Transferrin receptor protein 1 Proteins 0.000 description 1
• 108010021625 Immunoglobulin Fragments Proteins 0.000 description 1
• 102000008394 Immunoglobulin Fragments Human genes 0.000 description 1
• 102000006496 Immunoglobulin Heavy Chains Human genes 0.000 description 1
• 108010019476 Immunoglobulin Heavy Chains Proteins 0.000 description 1
• 206010062016 Immunosuppression Diseases 0.000 description 1
• 206010061218 Inflammation Diseases 0.000 description 1
• 108020005350 Initiator Codon Proteins 0.000 description 1
• 102000014150 Interferons Human genes 0.000 description 1
• 108010050904 Interferons Proteins 0.000 description 1
• 108010065805 Interleukin-12 Proteins 0.000 description 1
• 102000013462 Interleukin-12 Human genes 0.000 description 1
• 108010002586 Interleukin-7 Proteins 0.000 description 1
• 102100021592 Interleukin-7 Human genes 0.000 description 1
• 108090001007 Interleukin-8 Proteins 0.000 description 1
• 239000007760 Iscove's Modified Dulbecco's Medium Substances 0.000 description 1
• WTDRDQBEARUVNC-LURJTMIESA-N L-DOPA Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C(O)=C1 WTDRDQBEARUVNC-LURJTMIESA-N 0.000 description 1
• 125000000998 L-alanino group Chemical group [H]N([*])[C@](C([H])([H])[H])([H])C(=O)O[H] 0.000 description 1
• FSBIGDSBMBYOPN-VKHMYHEASA-N L-canavanine Chemical compound OC(=O)[C@@H](N)CCONC(N)=N FSBIGDSBMBYOPN-VKHMYHEASA-N 0.000 description 1
• UKAUYVFTDYCKQA-VKHMYHEASA-N L-homoserine Chemical compound OC(=O)[C@@H](N)CCO UKAUYVFTDYCKQA-VKHMYHEASA-N 0.000 description 1
• 125000000393 L-methionino group Chemical group [H]OC(=O)[C@@]([H])(N([H])[*])C([H])([H])C(SC([H])([H])[H])([H])[H] 0.000 description 1
• LRQKBLKVPFOOQJ-YFKPBYRVSA-N L-norleucine Chemical compound CCCC[C@H]([NH3+])C([O-])=O LRQKBLKVPFOOQJ-YFKPBYRVSA-N 0.000 description 1
• QIVBCDIJIAJPQS-VIFPVBQESA-N L-tryptophane Chemical compound C1=CC=C2C(C[C@H](N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-VIFPVBQESA-N 0.000 description 1
• GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 description 1
• ROHFNLRQFUQHCH-UHFFFAOYSA-N Leucine Natural products CC(C)CC(N)C(O)=O ROHFNLRQFUQHCH-UHFFFAOYSA-N 0.000 description 1
• 102100025584 Leukocyte immunoglobulin-like receptor subfamily B member 1 Human genes 0.000 description 1
• 206010058467 Lung neoplasm malignant Diseases 0.000 description 1
• 102000008072 Lymphokines Human genes 0.000 description 1
• 108010074338 Lymphokines Proteins 0.000 description 1
• 206010025323 Lymphomas Diseases 0.000 description 1
• 102100035304 Lymphotactin Human genes 0.000 description 1
• 108700005092 MHC Class II Genes Proteins 0.000 description 1
• 108010066345 MHC binding peptide Proteins 0.000 description 1
• 102000007651 Macrophage Colony-Stimulating Factor Human genes 0.000 description 1
• 108010046938 Macrophage Colony-Stimulating Factor Proteins 0.000 description 1
• 241000124008 Mammalia Species 0.000 description 1
• 102000012750 Membrane Glycoproteins Human genes 0.000 description 1
• 108010090054 Membrane Glycoproteins Proteins 0.000 description 1
• 102000003792 Metallothionein Human genes 0.000 description 1
• 108090000157 Metallothionein Proteins 0.000 description 1
• 241001183012 Modified Vaccinia Ankara virus Species 0.000 description 1
• 241000713869 Moloney murine leukemia virus Species 0.000 description 1
• 241000713333 Mouse mammary tumor virus Species 0.000 description 1
• 101100495074 Mus musculus Ccl2 gene Proteins 0.000 description 1
• 101100061857 Mus musculus Cxcl2 gene Proteins 0.000 description 1
• 241000699670 Mus sp. Species 0.000 description 1
• 241000187479 Mycobacterium tuberculosis Species 0.000 description 1
• 102400000569 Myeloperoxidase Human genes 0.000 description 1
• 108090000235 Myeloperoxidases Proteins 0.000 description 1
• CHJJGSNFBQVOTG-UHFFFAOYSA-N N-methyl-guanidine Natural products CNC(N)=N CHJJGSNFBQVOTG-UHFFFAOYSA-N 0.000 description 1
• 108091008604 NGF receptors Proteins 0.000 description 1
• 108010004217 Natural Cytotoxicity Triggering Receptor 1 Proteins 0.000 description 1
• 102100032870 Natural cytotoxicity triggering receptor 1 Human genes 0.000 description 1
• FSBIGDSBMBYOPN-UHFFFAOYSA-N O-guanidino-DL-homoserine Natural products OC(=O)C(N)CCON=C(N)N FSBIGDSBMBYOPN-UHFFFAOYSA-N 0.000 description 1
• 108010038807 Oligopeptides Proteins 0.000 description 1
• 102000015636 Oligopeptides Human genes 0.000 description 1
• 101710160107 Outer membrane protein A Proteins 0.000 description 1
• 229910019142 PO4 Inorganic materials 0.000 description 1
• 239000002033 PVDF binder Substances 0.000 description 1
• 102000004503 Perforin Human genes 0.000 description 1
• 108010056995 Perforin Proteins 0.000 description 1
• KHGNFPUMBJSZSM-UHFFFAOYSA-N Perforine Natural products COC1=C2CCC(O)C(CCC(C)(C)O)(OC)C2=NC2=C1C=CO2 KHGNFPUMBJSZSM-UHFFFAOYSA-N 0.000 description 1
• 201000005702 Pertussis Diseases 0.000 description 1
• 208000007452 Plasmacytoma Diseases 0.000 description 1
• 101001037767 Plasmodium falciparum (isolate FC27 / Papua New Guinea) Small histidine-alanine-rich protein Proteins 0.000 description 1
• 108010039918 Polylysine Proteins 0.000 description 1
• 229920001213 Polysorbate 20 Polymers 0.000 description 1
• 206010060862 Prostate cancer Diseases 0.000 description 1
• 102000007327 Protamines Human genes 0.000 description 1
• 108010007568 Protamines Proteins 0.000 description 1
• 108010076504 Protein Sorting Signals Proteins 0.000 description 1
• LCTONWCANYUPML-UHFFFAOYSA-M Pyruvate Chemical compound CC(=O)C([O-])=O LCTONWCANYUPML-UHFFFAOYSA-M 0.000 description 1
• 101000679843 Rattus norvegicus Tumor necrosis factor receptor superfamily member 4 Proteins 0.000 description 1
• 102100030086 Receptor tyrosine-protein kinase erbB-2 Human genes 0.000 description 1
• 108010008281 Recombinant Fusion Proteins Proteins 0.000 description 1
• 102000007056 Recombinant Fusion Proteins Human genes 0.000 description 1
• 241000283984 Rodentia Species 0.000 description 1
• 239000006146 Roswell Park Memorial Institute medium Substances 0.000 description 1
• 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
• 206010039491 Sarcoma Diseases 0.000 description 1
• BQCADISMDOOEFD-UHFFFAOYSA-N Silver Chemical compound [Ag] BQCADISMDOOEFD-UHFFFAOYSA-N 0.000 description 1
• 241000700584 Simplexvirus Species 0.000 description 1
• 108010073771 Soybean Proteins Proteins 0.000 description 1
• 229920002472 Starch Polymers 0.000 description 1
• 108091081024 Start codon Proteins 0.000 description 1
• 108010090804 Streptavidin Proteins 0.000 description 1
• 102100027213 T-cell-specific surface glycoprotein CD28 Human genes 0.000 description 1
• 101150052863 THY1 gene Proteins 0.000 description 1
• 206010048669 Terminal state Diseases 0.000 description 1
• RYYWUUFWQRZTIU-UHFFFAOYSA-N Thiophosphoric acid Chemical class OP(O)(S)=O RYYWUUFWQRZTIU-UHFFFAOYSA-N 0.000 description 1
• 102000004338 Transferrin Human genes 0.000 description 1
• 108090000901 Transferrin Proteins 0.000 description 1
• 102100026144 Transferrin receptor protein 1 Human genes 0.000 description 1
• 239000007983 Tris buffer Substances 0.000 description 1
• QIVBCDIJIAJPQS-UHFFFAOYSA-N Tryptophan Natural products C1=CC=C2C(CC(N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-UHFFFAOYSA-N 0.000 description 1
• 102100033725 Tumor necrosis factor receptor superfamily member 16 Human genes 0.000 description 1
• 208000007097 Urinary Bladder Neoplasms Diseases 0.000 description 1
• 208000036142 Viral infection Diseases 0.000 description 1
• 208000027418 Wounds and injury Diseases 0.000 description 1
• UZQJVUCHXGYFLQ-AYDHOLPZSA-N [(2s,3r,4s,5r,6r)-4-[(2s,3r,4s,5r,6r)-4-[(2r,3r,4s,5r,6r)-4-[(2s,3r,4s,5r,6r)-3,5-dihydroxy-6-(hydroxymethyl)-4-[(2s,3r,4s,5s,6r)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxyoxan-2-yl]oxy-3,5-dihydroxy-6-(hydroxymethyl)oxan-2-yl]oxy-3,5-dihydroxy-6-(hy Chemical compound O([C@H]1[C@H](O)[C@@H](CO)O[C@H]([C@@H]1O)O[C@H]1[C@H](O)[C@@H](CO)O[C@H]([C@@H]1O)O[C@H]1CC[C@]2(C)[C@H]3CC=C4[C@@]([C@@]3(CC[C@H]2[C@@]1(C=O)C)C)(C)CC(O)[C@]1(CCC(CC14)(C)C)C(=O)O[C@H]1[C@@H]([C@@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O[C@H]4[C@@H]([C@@H](O[C@H]5[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O5)O)[C@H](O)[C@@H](CO)O4)O)[C@H](O)[C@@H](CO)O3)O)[C@H](O)[C@@H](CO)O2)O)[C@H](O)[C@@H](CO)O1)O)[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O UZQJVUCHXGYFLQ-AYDHOLPZSA-N 0.000 description 1
• JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 1
• 238000009825 accumulation Methods 0.000 description 1
• 239000012190 activator Substances 0.000 description 1
• 239000004480 active ingredient Substances 0.000 description 1
• 239000013543 active substance Substances 0.000 description 1
• 230000033289 adaptive immune response Effects 0.000 description 1
• 239000000654 additive Substances 0.000 description 1
• 229960005305 adenosine Drugs 0.000 description 1
• 238000001042 affinity chromatography Methods 0.000 description 1
• 230000001270 agonistic effect Effects 0.000 description 1
• 230000002152 alkylating effect Effects 0.000 description 1
• 230000000961 alloantigen Effects 0.000 description 1
• VREFGVBLTWBCJP-UHFFFAOYSA-N alprazolam Chemical compound C12=CC(Cl)=CC=C2N2C(C)=NN=C2CN=C1C1=CC=CC=C1 VREFGVBLTWBCJP-UHFFFAOYSA-N 0.000 description 1
• 229940037003 alum Drugs 0.000 description 1
• 150000001409 amidines Chemical class 0.000 description 1
• 230000003321 amplification Effects 0.000 description 1
• 229940051881 anilide analgesics and antipyretics Drugs 0.000 description 1
• 150000003931 anilides Chemical class 0.000 description 1
• 210000004102 animal cell Anatomy 0.000 description 1
• 230000002494 anti-cea effect Effects 0.000 description 1
• 230000000340 anti-metabolite Effects 0.000 description 1
• 229940100197 antimetabolite Drugs 0.000 description 1
• 239000002256 antimetabolite Substances 0.000 description 1
• 239000003963 antioxidant agent Substances 0.000 description 1
• 230000005975 antitumor immune response Effects 0.000 description 1
• 230000006907 apoptotic process Effects 0.000 description 1
• 238000000149 argon plasma sintering Methods 0.000 description 1
• 230000002238 attenuated effect Effects 0.000 description 1
• 230000003416 augmentation Effects 0.000 description 1
• 230000003190 augmentative effect Effects 0.000 description 1
• 244000052616 bacterial pathogen Species 0.000 description 1
• 210000003651 basophil Anatomy 0.000 description 1
• 230000006399 behavior Effects 0.000 description 1
• 230000008901 benefit Effects 0.000 description 1
• 229940049706 benzodiazepine Drugs 0.000 description 1
• 125000003310 benzodiazepinyl group Chemical group N1N=C(C=CC2=C1C=CC=C2)* 0.000 description 1
• 150000001558 benzoic acid derivatives Chemical class 0.000 description 1
• IQFYYKKMVGJFEH-UHFFFAOYSA-N beta-L-thymidine Natural products O=C1NC(=O)C(C)=CN1C1OC(CO)C(O)C1 IQFYYKKMVGJFEH-UHFFFAOYSA-N 0.000 description 1
• DRTQHJPVMGBUCF-PSQAKQOGSA-N beta-L-uridine Natural products O[C@H]1[C@@H](O)[C@H](CO)O[C@@H]1N1C(=O)NC(=O)C=C1 DRTQHJPVMGBUCF-PSQAKQOGSA-N 0.000 description 1
• 238000004166 bioassay Methods 0.000 description 1
• 230000015572 biosynthetic process Effects 0.000 description 1
• 201000000053 blastoma Diseases 0.000 description 1
• 210000000601 blood cell Anatomy 0.000 description 1
• 210000001772 blood platelet Anatomy 0.000 description 1
• 210000001124 body fluid Anatomy 0.000 description 1
• 201000007295 breast benign neoplasm Diseases 0.000 description 1
• 210000001669 bursa of fabricius Anatomy 0.000 description 1
• 239000011575 calcium Substances 0.000 description 1
• 238000004364 calculation method Methods 0.000 description 1
• 230000000711 cancerogenic effect Effects 0.000 description 1
• 150000001720 carbohydrates Chemical class 0.000 description 1
• 235000014633 carbohydrates Nutrition 0.000 description 1
• 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 1
• 231100000315 carcinogenic Toxicity 0.000 description 1
• 239000005018 casein Substances 0.000 description 1
• BECPQYXYKAMYBN-UHFFFAOYSA-N casein, tech. Chemical compound NCCCCC(C(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(CC(C)C)N=C(O)C(CCC(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(C(C)O)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(COP(O)(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(N)CC1=CC=CC=C1 BECPQYXYKAMYBN-UHFFFAOYSA-N 0.000 description 1
• 235000021240 caseins Nutrition 0.000 description 1
• 230000020411 cell activation Effects 0.000 description 1
• 230000011712 cell development Effects 0.000 description 1
• 230000003915 cell function Effects 0.000 description 1
• 230000007910 cell fusion Effects 0.000 description 1
• 230000022534 cell killing Effects 0.000 description 1
• 230000004663 cell proliferation Effects 0.000 description 1
• 238000001516 cell proliferation assay Methods 0.000 description 1
• 230000033383 cell-cell recognition Effects 0.000 description 1
• 230000007969 cellular immunity Effects 0.000 description 1
• 239000001913 cellulose Substances 0.000 description 1
• 229920002678 cellulose Polymers 0.000 description 1
• 210000002230 centromere Anatomy 0.000 description 1
• 239000002738 chelating agent Substances 0.000 description 1
• 239000003638 chemical reducing agent Substances 0.000 description 1
• 239000005081 chemiluminescent agent Substances 0.000 description 1
• 239000002975 chemoattractant Substances 0.000 description 1
• 239000005482 chemotactic factor Substances 0.000 description 1
• 238000009104 chemotherapy regimen Methods 0.000 description 1
• 235000012000 cholesterol Nutrition 0.000 description 1
• 239000012539 chromatography resin Substances 0.000 description 1
• 239000013611 chromosomal DNA Substances 0.000 description 1
• 238000003759 clinical diagnosis Methods 0.000 description 1
• 210000001072 colon Anatomy 0.000 description 1
• 208000029742 colonic neoplasm Diseases 0.000 description 1
• 231100000026 common toxicity Toxicity 0.000 description 1
• 230000002596 correlated effect Effects 0.000 description 1
• 235000012343 cottonseed oil Nutrition 0.000 description 1
• 230000009260 cross reactivity Effects 0.000 description 1
• 239000002577 cryoprotective agent Substances 0.000 description 1
• UHDGCWIWMRVCDJ-ZAKLUEHWSA-N cytidine Chemical compound O=C1N=C(N)C=CN1[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O1 UHDGCWIWMRVCDJ-ZAKLUEHWSA-N 0.000 description 1
• 230000016396 cytokine production Effects 0.000 description 1
• 229940104302 cytosine Drugs 0.000 description 1
• 229940127089 cytotoxic agent Drugs 0.000 description 1
• 239000002254 cytotoxic agent Substances 0.000 description 1
• 238000002784 cytotoxicity assay Methods 0.000 description 1
• 231100000263 cytotoxicity test Toxicity 0.000 description 1
• 239000002619 cytotoxin Substances 0.000 description 1
• 238000013499 data model Methods 0.000 description 1
• 230000004041 dendritic cell maturation Effects 0.000 description 1
• 238000000432 density-gradient centrifugation Methods 0.000 description 1
• 239000005549 deoxyribonucleoside Substances 0.000 description 1
• SWSQBOPZIKWTGO-UHFFFAOYSA-N dimethylaminoamidine Natural products CN(C)C(N)=N SWSQBOPZIKWTGO-UHFFFAOYSA-N 0.000 description 1
• PXLIDIMHPNPGMH-PJWPDVOUSA-N disodium;dioxido(dioxo)chromium-51 Chemical compound [Na+].[Na+].[O-][51Cr]([O-])(=O)=O PXLIDIMHPNPGMH-PJWPDVOUSA-N 0.000 description 1
• 210000003162 effector t lymphocyte Anatomy 0.000 description 1
• 201000008184 embryoma Diseases 0.000 description 1
• 239000000839 emulsion Substances 0.000 description 1
• 230000002121 endocytic effect Effects 0.000 description 1
• 230000012202 endocytosis Effects 0.000 description 1
• 230000003511 endothelial effect Effects 0.000 description 1
• 239000002158 endotoxin Substances 0.000 description 1
• 230000002708 enhancing effect Effects 0.000 description 1
• 230000007613 environmental effect Effects 0.000 description 1
• 230000002255 enzymatic effect Effects 0.000 description 1
• 210000003979 eosinophil Anatomy 0.000 description 1
• 230000010502 episomal replication Effects 0.000 description 1
• 210000003743 erythrocyte Anatomy 0.000 description 1
• 230000005713 exacerbation Effects 0.000 description 1
• 239000013604 expression vector Substances 0.000 description 1
• 108010052621 fas Receptor Proteins 0.000 description 1
• 102000018823 fas Receptor Human genes 0.000 description 1
• 230000009123 feedback regulation Effects 0.000 description 1
• 238000000855 fermentation Methods 0.000 description 1
• 230000004151 fermentation Effects 0.000 description 1
• 210000004700 fetal blood Anatomy 0.000 description 1
• 108700014844 flt3 ligand Proteins 0.000 description 1
• 239000012530 fluid Substances 0.000 description 1
• 238000001917 fluorescence detection Methods 0.000 description 1
• 238000001943 fluorescence-activated cell sorting Methods 0.000 description 1
• 230000037433 frameshift Effects 0.000 description 1
• 125000000524 functional group Chemical group 0.000 description 1
• UIWYJDYFSGRHKR-UHFFFAOYSA-N gadolinium atom Chemical compound [Gd] UIWYJDYFSGRHKR-UHFFFAOYSA-N 0.000 description 1
• IZOOGPBRAOKZFK-UHFFFAOYSA-K gadopentetate Chemical compound [Gd+3].OC(=O)CN(CC([O-])=O)CCN(CC([O-])=O)CCN(CC(O)=O)CC([O-])=O IZOOGPBRAOKZFK-UHFFFAOYSA-K 0.000 description 1
• 230000002496 gastric effect Effects 0.000 description 1
• 230000030279 gene silencing Effects 0.000 description 1
• 238000001415 gene therapy Methods 0.000 description 1
• 238000010353 genetic engineering Methods 0.000 description 1
• 239000011521 glass Substances 0.000 description 1
• 239000003862 glucocorticoid Substances 0.000 description 1
• 229960003180 glutathione Drugs 0.000 description 1
• ZNNLBTZKUZBEKO-UHFFFAOYSA-N glyburide Chemical compound COC1=CC=C(Cl)C=C1C(=O)NCCC1=CC=C(S(=O)(=O)NC(=O)NC2CCCCC2)C=C1 ZNNLBTZKUZBEKO-UHFFFAOYSA-N 0.000 description 1
• 150000004676 glycans Chemical class 0.000 description 1
• 230000005484 gravity Effects 0.000 description 1
• 229940029575 guanosine Drugs 0.000 description 1
• 230000003394 haemopoietic effect Effects 0.000 description 1
• 238000003306 harvesting Methods 0.000 description 1
• 201000003911 head and neck carcinoma Diseases 0.000 description 1
• 210000003630 histaminocyte Anatomy 0.000 description 1
• 102000055276 human IL3 Human genes 0.000 description 1
• 235000020256 human milk Nutrition 0.000 description 1
• 210000004251 human milk Anatomy 0.000 description 1
• 150000003840 hydrochlorides Chemical class 0.000 description 1
• 229960000890 hydrocortisone Drugs 0.000 description 1
• 210000001822 immobilized cell Anatomy 0.000 description 1
• 230000002519 immonomodulatory effect Effects 0.000 description 1
• 239000012642 immune effector Substances 0.000 description 1
• 230000036737 immune function Effects 0.000 description 1
• 230000008105 immune reaction Effects 0.000 description 1
• 229940124452 immunizing agent Drugs 0.000 description 1
• 238000003119 immunoblot Methods 0.000 description 1
• 238000003125 immunofluorescent labeling Methods 0.000 description 1
• 229940072221 immunoglobulins Drugs 0.000 description 1
• 229940121354 immunomodulator Drugs 0.000 description 1
• 230000004957 immunoregulator effect Effects 0.000 description 1
• 230000003308 immunostimulating effect Effects 0.000 description 1
• 230000001506 immunosuppresive effect Effects 0.000 description 1
• 238000011065 in-situ storage Methods 0.000 description 1
• 238000010348 incorporation Methods 0.000 description 1
• 230000008595 infiltration Effects 0.000 description 1
• 238000001764 infiltration Methods 0.000 description 1
• 230000002757 inflammatory effect Effects 0.000 description 1
• 230000004054 inflammatory process Effects 0.000 description 1
• 239000003112 inhibitor Substances 0.000 description 1
• 208000014674 injury Diseases 0.000 description 1
• 229910052500 inorganic mineral Inorganic materials 0.000 description 1
• 230000002452 interceptive effect Effects 0.000 description 1
• 229940079322 interferon Drugs 0.000 description 1
• 229940047124 interferons Drugs 0.000 description 1
• 229940076264 interleukin-3 Drugs 0.000 description 1
• 210000000936 intestine Anatomy 0.000 description 1
• 230000010039 intracellular degradation Effects 0.000 description 1
• 230000003834 intracellular effect Effects 0.000 description 1
• 230000010189 intracellular transport Effects 0.000 description 1
• 238000007917 intracranial administration Methods 0.000 description 1
• 238000007918 intramuscular administration Methods 0.000 description 1
• 239000007928 intraperitoneal injection Substances 0.000 description 1
• 230000009545 invasion Effects 0.000 description 1
• 208000028867 ischemia Diseases 0.000 description 1
• 229960000310 isoleucine Drugs 0.000 description 1
• AGPKZVBTJJNPAG-UHFFFAOYSA-N isoleucine Natural products CCC(C)C(N)C(O)=O AGPKZVBTJJNPAG-UHFFFAOYSA-N 0.000 description 1
• 230000002147 killing effect Effects 0.000 description 1
• 101150066555 lacZ gene Proteins 0.000 description 1
• 239000008101 lactose Substances 0.000 description 1
• 210000001821 langerhans cell Anatomy 0.000 description 1
• 231100001231 less toxic Toxicity 0.000 description 1
• 229960004502 levodopa Drugs 0.000 description 1
• GZQKNULLWNGMCW-PWQABINMSA-N lipid A (E. coli) Chemical compound O1[C@H](CO)[C@@H](OP(O)(O)=O)[C@H](OC(=O)C[C@@H](CCCCCCCCCCC)OC(=O)CCCCCCCCCCCCC)[C@@H](NC(=O)C[C@@H](CCCCCCCCCCC)OC(=O)CCCCCCCCCCC)[C@@H]1OC[C@@H]1[C@@H](O)[C@H](OC(=O)C[C@H](O)CCCCCCCCCCC)[C@@H](NC(=O)C[C@H](O)CCCCCCCCCCC)[C@@H](OP(O)(O)=O)O1 GZQKNULLWNGMCW-PWQABINMSA-N 0.000 description 1
• 238000001638 lipofection Methods 0.000 description 1
• 229920006008 lipopolysaccharide Polymers 0.000 description 1
• 239000007788 liquid Substances 0.000 description 1
• 238000011068 loading method Methods 0.000 description 1
• 239000003589 local anesthetic agent Substances 0.000 description 1
• 229960005015 local anesthetics Drugs 0.000 description 1
• 230000004807 localization Effects 0.000 description 1
• 230000005923 long-lasting effect Effects 0.000 description 1
• 201000005296 lung carcinoma Diseases 0.000 description 1
• 208000020816 lung neoplasm Diseases 0.000 description 1
• 210000001165 lymph node Anatomy 0.000 description 1
• 210000005210 lymphoid organ Anatomy 0.000 description 1
• 210000003810 lymphokine-activated killer cell Anatomy 0.000 description 1
• 108010019677 lymphotactin Proteins 0.000 description 1
• 239000006166 lysate Substances 0.000 description 1
• 239000011777 magnesium Substances 0.000 description 1
• ZLNQQNXFFQJAID-UHFFFAOYSA-L magnesium carbonate Chemical compound [Mg+2].[O-]C([O-])=O ZLNQQNXFFQJAID-UHFFFAOYSA-L 0.000 description 1
• 239000001095 magnesium carbonate Substances 0.000 description 1
• 229910000021 magnesium carbonate Inorganic materials 0.000 description 1
• 235000019359 magnesium stearate Nutrition 0.000 description 1
• 239000006249 magnetic particle Substances 0.000 description 1
• 238000007885 magnetic separation Methods 0.000 description 1
• 230000003211 malignant effect Effects 0.000 description 1
• 238000013507 mapping Methods 0.000 description 1
• 210000003519 mature b lymphocyte Anatomy 0.000 description 1
• 235000013372 meat Nutrition 0.000 description 1
• MIKKOBKEXMRYFQ-WZTVWXICSA-N meglumine amidotrizoate Chemical compound C[NH2+]C[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO.CC(=O)NC1=C(I)C(NC(C)=O)=C(I)C(C([O-])=O)=C1I MIKKOBKEXMRYFQ-WZTVWXICSA-N 0.000 description 1
• 210000004779 membrane envelope Anatomy 0.000 description 1
• 239000002207 metabolite Substances 0.000 description 1
• 230000001394 metastastic effect Effects 0.000 description 1
• 206010061289 metastatic neoplasm Diseases 0.000 description 1
• MYWUZJCMWCOHBA-VIFPVBQESA-N methamphetamine Chemical compound CN[C@@H](C)CC1=CC=CC=C1 MYWUZJCMWCOHBA-VIFPVBQESA-N 0.000 description 1
• 238000001471 micro-filtration Methods 0.000 description 1
• 230000000813 microbial effect Effects 0.000 description 1
• 244000005700 microbiome Species 0.000 description 1
• 230000003278 mimic effect Effects 0.000 description 1
• 239000011707 mineral Substances 0.000 description 1
• 239000007758 minimum essential medium Substances 0.000 description 1
• 230000002438 mitochondrial effect Effects 0.000 description 1
• 238000007799 mixed lymphocyte reaction assay Methods 0.000 description 1
• 108091005601 modified peptides Proteins 0.000 description 1
• 230000009149 molecular binding Effects 0.000 description 1
• 229940035032 monophosphoryl lipid a Drugs 0.000 description 1
• PJUIMOJAAPLTRJ-UHFFFAOYSA-N monothioglycerol Chemical compound OCC(O)CS PJUIMOJAAPLTRJ-UHFFFAOYSA-N 0.000 description 1
• 210000004400 mucous membrane Anatomy 0.000 description 1
• 238000002887 multiple sequence alignment Methods 0.000 description 1
• 210000000822 natural killer cell Anatomy 0.000 description 1
• 210000005170 neoplastic cell Anatomy 0.000 description 1
• 210000003061 neural cell Anatomy 0.000 description 1
• 229910052757 nitrogen Inorganic materials 0.000 description 1
• 208000002154 non-small cell lung carcinoma Diseases 0.000 description 1
• 231100000252 nontoxic Toxicity 0.000 description 1
• 230000003000 nontoxic effect Effects 0.000 description 1
• 238000003199 nucleic acid amplification method Methods 0.000 description 1
• 238000007899 nucleic acid hybridization Methods 0.000 description 1
• 210000004940 nucleus Anatomy 0.000 description 1
• 235000015097 nutrients Nutrition 0.000 description 1
• 239000003921 oil Substances 0.000 description 1
• 235000019198 oils Nutrition 0.000 description 1
• 238000002515 oligonucleotide synthesis Methods 0.000 description 1
• 238000011275 oncology therapy Methods 0.000 description 1
• 210000003463 organelle Anatomy 0.000 description 1
• 150000002894 organic compounds Chemical class 0.000 description 1
• 230000002611 ovarian Effects 0.000 description 1
• LDCYZAJDBXYCGN-UHFFFAOYSA-N oxitriptan Natural products C1=C(O)C=C2C(CC(N)C(O)=O)=CNC2=C1 LDCYZAJDBXYCGN-UHFFFAOYSA-N 0.000 description 1
• 210000002741 palatine tonsil Anatomy 0.000 description 1
• 238000002638 palliative care Methods 0.000 description 1
• 210000000496 pancreas Anatomy 0.000 description 1
• 238000004091 panning Methods 0.000 description 1
• 238000007911 parenteral administration Methods 0.000 description 1
• 230000001575 pathological effect Effects 0.000 description 1
• 238000010647 peptide synthesis reaction Methods 0.000 description 1
• 229930192851 perforin Natural products 0.000 description 1
• 210000004976 peripheral blood cell Anatomy 0.000 description 1
• 230000002093 peripheral effect Effects 0.000 description 1
• 210000001539 phagocyte Anatomy 0.000 description 1
• 239000010452 phosphate Substances 0.000 description 1
• BZQFBWGGLXLEPQ-REOHCLBHSA-N phosphoserine Chemical compound OC(=O)[C@@H](N)COP(O)(O)=O BZQFBWGGLXLEPQ-REOHCLBHSA-N 0.000 description 1
• 230000008884 pinocytosis Effects 0.000 description 1
• 239000000902 placebo Substances 0.000 description 1
• 229940068196 placebo Drugs 0.000 description 1
• 210000004180 plasmocyte Anatomy 0.000 description 1
• 239000004033 plastic Substances 0.000 description 1
• 229920001983 poloxamer Polymers 0.000 description 1
• 229920000447 polyanionic polymer Polymers 0.000 description 1
• 229920000656 polylysine Polymers 0.000 description 1
• 238000003752 polymerase chain reaction Methods 0.000 description 1
• 102000054765 polymorphisms of proteins Human genes 0.000 description 1
• 229920005862 polyol Polymers 0.000 description 1
• 150000003077 polyols Chemical class 0.000 description 1
• 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 description 1
• 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 description 1
• 229920001282 polysaccharide Polymers 0.000 description 1
• 239000005017 polysaccharide Substances 0.000 description 1
• 229920002981 polyvinylidene fluoride Polymers 0.000 description 1
• 238000010837 poor prognosis Methods 0.000 description 1
• 238000001556 precipitation Methods 0.000 description 1
• 239000003755 preservative agent Substances 0.000 description 1
• 230000000770 proinflammatory effect Effects 0.000 description 1
• 230000001737 promoting effect Effects 0.000 description 1
• 210000002307 prostate Anatomy 0.000 description 1
• 201000001514 prostate carcinoma Diseases 0.000 description 1
• 229940048914 protamine Drugs 0.000 description 1
• 230000001681 protective effect Effects 0.000 description 1
• 238000002731 protein assay Methods 0.000 description 1
• 238000000159 protein binding assay Methods 0.000 description 1
• 238000000751 protein extraction Methods 0.000 description 1
• 210000001938 protoplast Anatomy 0.000 description 1
• 238000000746 purification Methods 0.000 description 1
• 238000011002 quantification Methods 0.000 description 1
• 230000005855 radiation Effects 0.000 description 1
• 239000000376 reactant Substances 0.000 description 1
• 230000036647 reaction Effects 0.000 description 1
• 238000010188 recombinant method Methods 0.000 description 1
• 102000037983 regulatory factors Human genes 0.000 description 1
• 108091008025 regulatory factors Proteins 0.000 description 1
• 230000004043 responsiveness Effects 0.000 description 1
• 108091008146 restriction endonucleases Proteins 0.000 description 1
• 230000001177 retroviral effect Effects 0.000 description 1
• 238000010839 reverse transcription Methods 0.000 description 1
• 239000002342 ribonucleoside Substances 0.000 description 1
• 238000005096 rolling process Methods 0.000 description 1
• CVHZOJJKTDOEJC-UHFFFAOYSA-N saccharin Chemical compound C1=CC=C2C(=O)NS(=O)(=O)C2=C1 CVHZOJJKTDOEJC-UHFFFAOYSA-N 0.000 description 1
• 229960002530 sargramostim Drugs 0.000 description 1
• 210000005212 secondary lymphoid organ Anatomy 0.000 description 1
• 238000004062 sedimentation Methods 0.000 description 1
• 230000001235 sensitizing effect Effects 0.000 description 1
• 230000011664 signaling Effects 0.000 description 1
• 229910052709 silver Inorganic materials 0.000 description 1
• 239000004332 silver Substances 0.000 description 1
• 150000003384 small molecules Chemical class 0.000 description 1
• 229950009396 sodium chromate (51cr) Drugs 0.000 description 1
• 229940001941 soy protein Drugs 0.000 description 1
• 210000000952 spleen Anatomy 0.000 description 1
• 239000008107 starch Substances 0.000 description 1
• 235000019698 starch Nutrition 0.000 description 1
• 239000000021 stimulant Substances 0.000 description 1
• 238000007920 subcutaneous administration Methods 0.000 description 1
• 230000002459 sustained effect Effects 0.000 description 1
• 238000003786 synthesis reaction Methods 0.000 description 1
• 230000009885 systemic effect Effects 0.000 description 1
• 239000000454 talc Substances 0.000 description 1
• 235000012222 talc Nutrition 0.000 description 1
• 229910052623 talc Inorganic materials 0.000 description 1
• 108091035539 telomere Proteins 0.000 description 1
• 102000055501 telomere Human genes 0.000 description 1
• 210000003411 telomere Anatomy 0.000 description 1
• 150000007970 thio esters Chemical class 0.000 description 1
• 229940104230 thymidine Drugs 0.000 description 1
• 230000002103 transcriptional effect Effects 0.000 description 1
• 239000012581 transferrin Substances 0.000 description 1
• 230000001960 triggered effect Effects 0.000 description 1
• LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 1
• 239000000439 tumor marker Substances 0.000 description 1
• 208000029729 tumor suppressor gene on chromosome 11 Diseases 0.000 description 1
• 241001529453 unidentified herpesvirus Species 0.000 description 1
• 241001430294 unidentified retrovirus Species 0.000 description 1
• DRTQHJPVMGBUCF-UHFFFAOYSA-N uracil arabinoside Natural products OC1C(O)C(CO)OC1N1C(=O)NC(=O)C=C1 DRTQHJPVMGBUCF-UHFFFAOYSA-N 0.000 description 1
• 229940045145 uridine Drugs 0.000 description 1
• 239000012646 vaccine adjuvant Substances 0.000 description 1
• 229940124931 vaccine adjuvant Drugs 0.000 description 1
• 108700000403 vaccinia-CEA vaccine Proteins 0.000 description 1
• 239000012808 vapor phase Substances 0.000 description 1
• 210000000264 venule Anatomy 0.000 description 1
• 230000035899 viability Effects 0.000 description 1
• 230000009385 viral infection Effects 0.000 description 1
• 229960004854 viral vaccine Drugs 0.000 description 1
• 239000011782 vitamin Substances 0.000 description 1
• 235000013343 vitamin Nutrition 0.000 description 1
• 229940088594 vitamin Drugs 0.000 description 1
• 229930003231 vitamin Natural products 0.000 description 1
• XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
• 230000003442 weekly effect Effects 0.000 description 1
• 229940053867 xeloda Drugs 0.000 description 1