AU2004285928B2 - Process for purifying proteins in a hydrophobic interaction chromatography flow-through fraction - Google Patents

Process for purifying proteins in a hydrophobic interaction chromatography flow-through fraction Download PDF

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Publication number
AU2004285928B2
AU2004285928B2 AU2004285928A AU2004285928A AU2004285928B2 AU 2004285928 B2 AU2004285928 B2 AU 2004285928B2 AU 2004285928 A AU2004285928 A AU 2004285928A AU 2004285928 A AU2004285928 A AU 2004285928A AU 2004285928 B2 AU2004285928 B2 AU 2004285928B2
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AU
Australia
Prior art keywords
protein
target protein
adsorbent
flow
hydrophobic
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AU2004285928A
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English (en)
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AU2004285928A1 (en
Inventor
Sanchayita Ghose
Abhinav A. Shukla
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Amgen Inc
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Amgen Inc
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Application filed by Amgen Inc filed Critical Amgen Inc
Publication of AU2004285928A1 publication Critical patent/AU2004285928A1/en
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/20—Partition-, reverse-phase or hydrophobic interaction chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/30—Non-immunoglobulin-derived peptide or protein having an immunoglobulin constant or Fc region, or a fragment thereof, attached thereto

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Analytical Chemistry (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
AU2004285928A 2003-10-24 2004-10-22 Process for purifying proteins in a hydrophobic interaction chromatography flow-through fraction Active AU2004285928B2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US51448603P 2003-10-24 2003-10-24
US60/514,486 2003-10-24
PCT/US2004/035007 WO2005042569A1 (en) 2003-10-24 2004-10-22 Process for purifying proteins in a hydrophobic interaction chromatography flow-through fraction

Publications (2)

Publication Number Publication Date
AU2004285928A1 AU2004285928A1 (en) 2005-05-12
AU2004285928B2 true AU2004285928B2 (en) 2012-02-02

Family

ID=34549339

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2004285928A Active AU2004285928B2 (en) 2003-10-24 2004-10-22 Process for purifying proteins in a hydrophobic interaction chromatography flow-through fraction

Country Status (8)

Country Link
US (1) US7427659B2 (es)
EP (1) EP1687328B1 (es)
AT (1) ATE462716T1 (es)
AU (1) AU2004285928B2 (es)
CA (1) CA2542951C (es)
DE (1) DE602004026343D1 (es)
ES (1) ES2342539T3 (es)
WO (1) WO2005042569A1 (es)

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KR101660575B1 (ko) 2005-03-11 2016-09-27 와이어쓰 엘엘씨 약한 분배성 크로마토그래피법
US8114832B2 (en) 2005-07-13 2012-02-14 Crossbeta Biosciences B.V. Method for detecting and/or removing a protein comprising a cross-beta structure from a pharmaceutical composition
US20070015133A1 (en) * 2005-07-13 2007-01-18 Umc Utrecht Holding B.V. Method for detecting and/or removing protein and/or peptide comprising a cross-beta structure from an aqueous solution comprising a protein
EP1910844B1 (en) 2005-07-13 2012-04-18 Crossbeta Biosciences B.V. Cross-beta structure binding compounds
US7566401B2 (en) * 2005-10-03 2009-07-28 Kelm Mark A Process for separating and isolating xanthines, individual polar protic monomers, and polar protic oligomers
KR101398713B1 (ko) 2005-12-20 2014-06-12 브리스톨-마이어스 스큅 컴퍼니 조성물 및 조성물의 제조 방법
AR058568A1 (es) 2005-12-20 2008-02-13 Bristol Myers Squibb Co Metodos para producir una composicion con moleculas ctla4-ig a partir de un medio de cultivo
EP2245049B1 (en) 2008-01-10 2015-05-20 Research Development Foundation Vaccines and diagnostics for the ehrlichioses
US8188242B2 (en) 2008-04-08 2012-05-29 Bio-Rad Laboratories, Inc. Chromatography purification of antibodies
WO2010009388A1 (en) * 2008-07-18 2010-01-21 Talecris Biotherapeutics, Inc. Method of preparing alpha-1 proteinase inhibitor
JP2012531428A (ja) 2009-06-25 2012-12-10 アムジエン・インコーポレーテツド 哺乳類以外の系で発現されるタンパク質の捕獲精製プロセス
US20110150861A1 (en) * 2009-10-30 2011-06-23 Abbott Laboratories Sorf constructs and multiple gene expression
WO2012082933A1 (en) 2010-12-15 2012-06-21 Baxter International, Inc. Eluate collection using conductivity gradient
EP2702077A2 (en) 2011-04-27 2014-03-05 AbbVie Inc. Methods for controlling the galactosylation profile of recombinantly-expressed proteins
US9783570B2 (en) 2011-07-01 2017-10-10 Hoffmann-La Roche Inc. Method for separation of monomeric polypeptides from aggregated polypeptides
CN103814044A (zh) 2011-07-08 2014-05-21 默沙东公司 纯化fc-融合蛋白的方法
JP6223338B2 (ja) 2011-08-17 2017-11-01 アレス トレーディング ソシエテ アノニム 活性型ＴＮＦＲ−Ｆｃ融合タンパク質を製造する方法
SG10201701224UA (en) 2012-03-12 2017-04-27 Merck Patent Gmbh Removal of protein aggregates from biopharmaceutical preparations in a flowthrough mode
US9067990B2 (en) 2013-03-14 2015-06-30 Abbvie, Inc. Protein purification using displacement chromatography
US9181572B2 (en) 2012-04-20 2015-11-10 Abbvie, Inc. Methods to modulate lysine variant distribution
US9334319B2 (en) 2012-04-20 2016-05-10 Abbvie Inc. Low acidic species compositions
US9249182B2 (en) * 2012-05-24 2016-02-02 Abbvie, Inc. Purification of antibodies using hydrophobic interaction chromatography
KR20150043523A (ko) 2012-09-02 2015-04-22 애브비 인코포레이티드 단백질 불균일성의 제어 방법
US9512214B2 (en) 2012-09-02 2016-12-06 Abbvie, Inc. Methods to control protein heterogeneity
SG11201507230PA (en) 2013-03-12 2015-10-29 Abbvie Inc Human antibodies that bind human tnf-alpha and methods of preparing the same
US10023608B1 (en) 2013-03-13 2018-07-17 Amgen Inc. Protein purification methods to remove impurities
US9017687B1 (en) 2013-10-18 2015-04-28 Abbvie, Inc. Low acidic species compositions and methods for producing and using the same using displacement chromatography
US8921526B2 (en) 2013-03-14 2014-12-30 Abbvie, Inc. Mutated anti-TNFα antibodies and methods of their use
US9499614B2 (en) 2013-03-14 2016-11-22 Abbvie Inc. Methods for modulating protein glycosylation profiles of recombinant protein therapeutics using monosaccharides and oligosaccharides
WO2014145208A1 (en) 2013-03-15 2014-09-18 Biogen Idec Ma Inc. Hydrophobic interaction protein chromatography under no-salt conditions
US9598667B2 (en) 2013-10-04 2017-03-21 Abbvie Inc. Use of metal ions for modulation of protein glycosylation profiles of recombinant proteins
US8946395B1 (en) 2013-10-18 2015-02-03 Abbvie Inc. Purification of proteins using hydrophobic interaction chromatography
US9085618B2 (en) 2013-10-18 2015-07-21 Abbvie, Inc. Low acidic species compositions and methods for producing and using the same
US9181337B2 (en) 2013-10-18 2015-11-10 Abbvie, Inc. Modulated lysine variant species compositions and methods for producing and using the same
US20150139988A1 (en) 2013-11-15 2015-05-21 Abbvie, Inc. Glycoengineered binding protein compositions
ES2810232T3 (es) 2013-12-12 2021-03-08 Emd Millipore Corp Método de separación de proteína monomérica de agregados de proteínas con una membrana porosa que comprende un copolímero de acrilamida de bencilo y acrilamida
JP7037275B2 (ja) * 2014-03-04 2022-03-16 メルク パテント ゲゼルシャフト ミット ベシュレンクテル ハフツング ロバストな抗体精製
AR102198A1 (es) 2014-10-09 2017-02-08 Regeneron Pharma Proceso para reducir partículas subvisibles en una formulación farmacéutica
JP2019529350A (ja) 2016-08-16 2019-10-17 リジェネロン・ファーマシューティカルズ・インコーポレイテッドＲｅｇｅｎｅｒｏｎ Ｐｈａｒｍａｃｅｕｔｉｃａｌｓ， Ｉｎｃ． 混合物から個々の抗体を定量する方法
HUE059631T2 (hu) 2016-10-25 2022-12-28 Regeneron Pharma Eljárások és összeállítások kromatográfiás adatelemzéshez
DK3668985T3 (da) 2017-08-17 2021-09-20 Just Evotec Biologics Inc Fremgangsmåde til rensning af glycosyleret protein fra værtscellegalectiner og andre urenheder
TW202005694A (zh) 2018-07-02 2020-02-01 美商里珍納龍藥品有限公司 自混合物製備多肽之系統及方法
MX2023006998A (es) 2020-12-22 2023-06-26 Amgen Inc Metodo de cultivo celular.
WO2023215750A2 (en) 2022-05-02 2023-11-09 Regeneron Pharmaceuticals, Inc. Methods for reducing lipase activity

Citations (5)

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US5468847A (en) * 1994-03-10 1995-11-21 Minnesota Mining And Manufacturing Company Method of isolating and purifying a biomacromolecule
WO1996033208A1 (en) * 1995-04-20 1996-10-24 Genentech, Inc. Antibody purification by low-ph hydrophobic interaction chromatoggraphy
US6005082A (en) * 1994-11-14 1999-12-21 Genetics Institute, Inc. Process for purification of factor VIII
WO2000059927A1 (en) * 1999-04-01 2000-10-12 Cantab Pharmaceuticals Research Limited Purification of biological preparations
US20030009025A1 (en) * 1993-09-21 2003-01-09 Smith Leonard A. Recombinant vaccine against botulinum neurotoxin

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US5463029A (en) * 1992-11-23 1995-10-31 Immunex Corporation Purification of fusion proteins comprising GM-CSF and IL-3
US5429746A (en) 1994-02-22 1995-07-04 Smith Kline Beecham Corporation Antibody purification
ES2266188T3 (es) * 2000-03-27 2007-03-01 Genetics Institute, Llc Procedimientos de purificacion de proteinas altamente anionicas.
ES2342539T3 (es) * 2003-10-24 2010-07-08 Amgen, Inc. Proceso para la purificacion de proteinas en una fraccion de flujo-directo de cromatografia de interaccion hidrofobica.

Patent Citations (5)

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Publication number Priority date Publication date Assignee Title
US20030009025A1 (en) * 1993-09-21 2003-01-09 Smith Leonard A. Recombinant vaccine against botulinum neurotoxin
US5468847A (en) * 1994-03-10 1995-11-21 Minnesota Mining And Manufacturing Company Method of isolating and purifying a biomacromolecule
US6005082A (en) * 1994-11-14 1999-12-21 Genetics Institute, Inc. Process for purification of factor VIII
WO1996033208A1 (en) * 1995-04-20 1996-10-24 Genentech, Inc. Antibody purification by low-ph hydrophobic interaction chromatoggraphy
WO2000059927A1 (en) * 1999-04-01 2000-10-12 Cantab Pharmaceuticals Research Limited Purification of biological preparations

Non-Patent Citations (2)

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Title
SCHOEL, BERND et al. 'Hydrophobic interaction chromatography for the purification of a mycobacterial heat shock protein of relative molecular mass 60 000.' Journal of Chromatography. 1991, Vol. 587, pages 19-23 *
SCHOEL, BERND et al. 'Hydrophobic interaction for the purification of cytolytic bacterial toxins.' Journal of Chromatography A. 1994, Vol. 667, pages 131-139 *

Also Published As

Publication number Publication date
WO2005042569A1 (en) 2005-05-12
US20050136521A1 (en) 2005-06-23
EP1687328B1 (en) 2010-03-31
ATE462716T1 (de) 2010-04-15
CA2542951C (en) 2016-08-30
AU2004285928A1 (en) 2005-05-12
EP1687328A4 (en) 2006-11-15
EP1687328A1 (en) 2006-08-09
ES2342539T3 (es) 2010-07-08
CA2542951A1 (en) 2005-05-12
US7427659B2 (en) 2008-09-23
DE602004026343D1 (de) 2010-05-12

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