AU2004285928B2 - Process for purifying proteins in a hydrophobic interaction chromatography flow-through fraction - Google Patents

Process for purifying proteins in a hydrophobic interaction chromatography flow-through fraction Download PDF

Info

Publication number
AU2004285928B2
AU2004285928B2 AU2004285928A AU2004285928A AU2004285928B2 AU 2004285928 B2 AU2004285928 B2 AU 2004285928B2 AU 2004285928 A AU2004285928 A AU 2004285928A AU 2004285928 A AU2004285928 A AU 2004285928A AU 2004285928 B2 AU2004285928 B2 AU 2004285928B2
Authority
AU
Australia
Prior art keywords
protein
target protein
adsorbent
flow
hydrophobic
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
AU2004285928A
Other languages
English (en)
Other versions
AU2004285928A1 (en
Inventor
Sanchayita Ghose
Abhinav A. Shukla
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Amgen Inc
Original Assignee
Amgen Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=34549339&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=AU2004285928(B2) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Amgen Inc filed Critical Amgen Inc
Publication of AU2004285928A1 publication Critical patent/AU2004285928A1/en
Application granted granted Critical
Publication of AU2004285928B2 publication Critical patent/AU2004285928B2/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/20—Partition-, reverse-phase or hydrophobic interaction chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/30—Non-immunoglobulin-derived peptide or protein having an immunoglobulin constant or Fc region, or a fragment thereof, attached thereto

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Analytical Chemistry (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
AU2004285928A 2003-10-24 2004-10-22 Process for purifying proteins in a hydrophobic interaction chromatography flow-through fraction Active AU2004285928B2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US51448603P 2003-10-24 2003-10-24
US60/514,486 2003-10-24
PCT/US2004/035007 WO2005042569A1 (en) 2003-10-24 2004-10-22 Process for purifying proteins in a hydrophobic interaction chromatography flow-through fraction

Publications (2)

Publication Number Publication Date
AU2004285928A1 AU2004285928A1 (en) 2005-05-12
AU2004285928B2 true AU2004285928B2 (en) 2012-02-02

Family

ID=34549339

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2004285928A Active AU2004285928B2 (en) 2003-10-24 2004-10-22 Process for purifying proteins in a hydrophobic interaction chromatography flow-through fraction

Country Status (8)

Country Link
US (1) US7427659B2 (es)
EP (1) EP1687328B1 (es)
AT (1) ATE462716T1 (es)
AU (1) AU2004285928B2 (es)
CA (1) CA2542951C (es)
DE (1) DE602004026343D1 (es)
ES (1) ES2342539T3 (es)
WO (1) WO2005042569A1 (es)

Families Citing this family (46)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1380290A1 (en) 2002-07-09 2004-01-14 Universitair Medisch Centrum Utrecht Cross-beta structure pathway and its therapeutic relevance
EP1687328B1 (en) * 2003-10-24 2010-03-31 Amgen, Inc. Process for purifying proteins in a hydrophobic interaction chromatography flow-through fraction
MX2007011129A (es) 2005-03-11 2007-11-06 Wyeth Corp Un metodo de cromatografia de particion debil.
US8114832B2 (en) * 2005-07-13 2012-02-14 Crossbeta Biosciences B.V. Method for detecting and/or removing a protein comprising a cross-beta structure from a pharmaceutical composition
CA2615028A1 (en) 2005-07-13 2007-01-18 Crossbeta Biosciences B.V. Cross-.beta. structure binding compounds
US20070015133A1 (en) * 2005-07-13 2007-01-18 Umc Utrecht Holding B.V. Method for detecting and/or removing protein and/or peptide comprising a cross-beta structure from an aqueous solution comprising a protein
US7566401B2 (en) * 2005-10-03 2009-07-28 Kelm Mark A Process for separating and isolating xanthines, individual polar protic monomers, and polar protic oligomers
TWI423986B (zh) 2005-12-20 2014-01-21 必治妥美雅史谷比公司 組合物及製造組合物之方法
AR058568A1 (es) 2005-12-20 2008-02-13 Bristol Myers Squibb Co Metodos para producir una composicion con moleculas ctla4-ig a partir de un medio de cultivo
CA2711499C (en) 2008-01-10 2018-09-25 Research Development Foundation Vaccines and diagnostics for the ehrlichioses
WO2009126603A1 (en) * 2008-04-08 2009-10-15 Bio-Rad Laboratories, Inc. Chromatography purification of antibodies
CN102099369A (zh) * 2008-07-18 2011-06-15 泰勒克里斯生物治疗学公司 制备α-1蛋白酶抑制剂的方法
EP2445924B2 (en) 2009-06-25 2023-12-13 Amgen Inc. Capture purification processes for proteins expressed in a non-mammalian system
AU2010313395A1 (en) 2009-10-30 2012-05-10 Abbvie Inc. sORF constructs and multiple gene expression
CA2821711C (en) 2010-12-15 2017-10-10 Baxter International Inc. Eluate collection using conductivity gradient
WO2012149197A2 (en) 2011-04-27 2012-11-01 Abbott Laboratories Methods for controlling the galactosylation profile of recombinantly-expressed proteins
SG10201605397PA (en) 2011-07-01 2016-08-30 Hoffmann La Roche Method For Separation Of Monomeric Polypeptides From Aggregated Polypeptides
CA2840951A1 (en) 2011-07-08 2013-01-17 Merck Sharp & Dohme Corp. Methods for purifying fc-fusion protein
KR101454316B1 (ko) 2011-08-17 2014-10-27 한화케미칼 주식회사 활성형 ＴＮＦＲ-Ｆｃ 융합 단백질을 제조하는 방법
SG10201701224UA (en) 2012-03-12 2017-04-27 Merck Patent Gmbh Removal of protein aggregates from biopharmaceutical preparations in a flowthrough mode
US9150645B2 (en) 2012-04-20 2015-10-06 Abbvie, Inc. Cell culture methods to reduce acidic species
US9067990B2 (en) 2013-03-14 2015-06-30 Abbvie, Inc. Protein purification using displacement chromatography
US9181572B2 (en) 2012-04-20 2015-11-10 Abbvie, Inc. Methods to modulate lysine variant distribution
WO2013176754A1 (en) * 2012-05-24 2013-11-28 Abbvie Inc. Novel purification of antibodies using hydrophobic interaction chromatography
US9206390B2 (en) 2012-09-02 2015-12-08 Abbvie, Inc. Methods to control protein heterogeneity
US9512214B2 (en) 2012-09-02 2016-12-06 Abbvie, Inc. Methods to control protein heterogeneity
WO2014143205A1 (en) 2013-03-12 2014-09-18 Abbvie Inc. Human antibodies that bind human tnf-alpha and methods of preparing the same
US10023608B1 (en) 2013-03-13 2018-07-17 Amgen Inc. Protein purification methods to remove impurities
WO2014151878A2 (en) 2013-03-14 2014-09-25 Abbvie Inc. Methods for modulating protein glycosylation profiles of recombinant protein therapeutics using monosaccharides and oligosacharides
US9017687B1 (en) 2013-10-18 2015-04-28 Abbvie, Inc. Low acidic species compositions and methods for producing and using the same using displacement chromatography
WO2014159579A1 (en) 2013-03-14 2014-10-02 Abbvie Inc. MUTATED ANTI-TNFα ANTIBODIES AND METHODS OF THEIR USE
EP2970378B1 (en) 2013-03-15 2021-05-26 Biogen MA Inc. Hydrophobic interaction protein chromatography under no-salt conditions
EP3052640A2 (en) 2013-10-04 2016-08-10 AbbVie Inc. Use of metal ions for modulation of protein glycosylation profiles of recombinant proteins
US9085618B2 (en) 2013-10-18 2015-07-21 Abbvie, Inc. Low acidic species compositions and methods for producing and using the same
US9181337B2 (en) 2013-10-18 2015-11-10 Abbvie, Inc. Modulated lysine variant species compositions and methods for producing and using the same
US8946395B1 (en) 2013-10-18 2015-02-03 Abbvie Inc. Purification of proteins using hydrophobic interaction chromatography
WO2015073884A2 (en) 2013-11-15 2015-05-21 Abbvie, Inc. Glycoengineered binding protein compositions
SG10201804200WA (en) 2013-12-12 2018-07-30 Emd Millipore Corp Protein separations using an acrylamide containing filter
KR102405382B1 (ko) * 2014-03-04 2022-06-03 메르크 파텐트 게엠베하 강건한 항체 정제
AR102198A1 (es) 2014-10-09 2017-02-08 Regeneron Pharma Proceso para reducir partículas subvisibles en una formulación farmacéutica
EP3500297A1 (en) 2016-08-16 2019-06-26 Regeneron Pharmaceuticals, Inc. Methods for quantitating individual antibodies from a mixture
PL3532838T3 (pl) 2016-10-25 2022-10-03 Regeneron Pharmaceuticals, Inc. Metody i systemy analizy danych chromatograficznych
EP3668985B1 (en) 2017-08-17 2021-06-16 Just-Evotec Biologics, Inc. Method of purifying glycosylated protein from host cell galectins and other contaminants
TW202005694A (zh) 2018-07-02 2020-02-01 美商里珍納龍藥品有限公司 自混合物製備多肽之系統及方法
WO2022140389A1 (en) 2020-12-22 2022-06-30 Amgen Inc. Cell culture method
WO2023215750A2 (en) 2022-05-02 2023-11-09 Regeneron Pharmaceuticals, Inc. Methods for reducing lipase activity

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5468847A (en) * 1994-03-10 1995-11-21 Minnesota Mining And Manufacturing Company Method of isolating and purifying a biomacromolecule
WO1996033208A1 (en) * 1995-04-20 1996-10-24 Genentech, Inc. Antibody purification by low-ph hydrophobic interaction chromatoggraphy
US6005082A (en) * 1994-11-14 1999-12-21 Genetics Institute, Inc. Process for purification of factor VIII
WO2000059927A1 (en) * 1999-04-01 2000-10-12 Cantab Pharmaceuticals Research Limited Purification of biological preparations
US20030009025A1 (en) * 1993-09-21 2003-01-09 Smith Leonard A. Recombinant vaccine against botulinum neurotoxin

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0449299A (ja) * 1990-06-14 1992-02-18 Tosoh Corp 免疫グロブリンフラグメントの精製法
US5463029A (en) * 1992-11-23 1995-10-31 Immunex Corporation Purification of fusion proteins comprising GM-CSF and IL-3
US5429746A (en) * 1994-02-22 1995-07-04 Smith Kline Beecham Corporation Antibody purification
WO2001072769A2 (en) 2000-03-27 2001-10-04 Genetics Institute, Llc Methods for purifying highly anionic proteins
EP1687328B1 (en) * 2003-10-24 2010-03-31 Amgen, Inc. Process for purifying proteins in a hydrophobic interaction chromatography flow-through fraction

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030009025A1 (en) * 1993-09-21 2003-01-09 Smith Leonard A. Recombinant vaccine against botulinum neurotoxin
US5468847A (en) * 1994-03-10 1995-11-21 Minnesota Mining And Manufacturing Company Method of isolating and purifying a biomacromolecule
US6005082A (en) * 1994-11-14 1999-12-21 Genetics Institute, Inc. Process for purification of factor VIII
WO1996033208A1 (en) * 1995-04-20 1996-10-24 Genentech, Inc. Antibody purification by low-ph hydrophobic interaction chromatoggraphy
WO2000059927A1 (en) * 1999-04-01 2000-10-12 Cantab Pharmaceuticals Research Limited Purification of biological preparations

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
SCHOEL, BERND et al. 'Hydrophobic interaction chromatography for the purification of a mycobacterial heat shock protein of relative molecular mass 60 000.' Journal of Chromatography. 1991, Vol. 587, pages 19-23 *
SCHOEL, BERND et al. 'Hydrophobic interaction for the purification of cytolytic bacterial toxins.' Journal of Chromatography A. 1994, Vol. 667, pages 131-139 *

Also Published As

Publication number Publication date
CA2542951C (en) 2016-08-30
EP1687328B1 (en) 2010-03-31
WO2005042569A1 (en) 2005-05-12
CA2542951A1 (en) 2005-05-12
EP1687328A1 (en) 2006-08-09
AU2004285928A1 (en) 2005-05-12
US7427659B2 (en) 2008-09-23
DE602004026343D1 (de) 2010-05-12
ATE462716T1 (de) 2010-04-15
ES2342539T3 (es) 2010-07-08
US20050136521A1 (en) 2005-06-23
EP1687328A4 (en) 2006-11-15

Similar Documents

Publication Publication Date Title
AU2004285928B2 (en) Process for purifying proteins in a hydrophobic interaction chromatography flow-through fraction
US7122641B2 (en) Methods for purifying protein
WO2007109163A2 (en) Wash buffer and method of using
US11192919B2 (en) Removal of leaked affinity purification ligand
US10023608B1 (en) Protein purification methods to remove impurities
EP2855501B1 (en) In situ restoration of apatite-based chromatography resins
US8273707B2 (en) Process for purifying proteins
WO2006060083A1 (en) Methods for refolding polypeptides
JP2013234154A (ja) 精製方法
JP2013184919A (ja) 精製方法
JP2013199454A (ja) 精製方法
JP2013227254A (ja) 精製方法

Legal Events

Date Code Title Description
FGA Letters patent sealed or granted (standard patent)