AU2004285928B2 - Process for purifying proteins in a hydrophobic interaction chromatography flow-through fraction - Google Patents

Process for purifying proteins in a hydrophobic interaction chromatography flow-through fraction Download PDF

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Publication number
AU2004285928B2
AU2004285928B2 AU2004285928A AU2004285928A AU2004285928B2 AU 2004285928 B2 AU2004285928 B2 AU 2004285928B2 AU 2004285928 A AU2004285928 A AU 2004285928A AU 2004285928 A AU2004285928 A AU 2004285928A AU 2004285928 B2 AU2004285928 B2 AU 2004285928B2
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AU
Australia
Prior art keywords
protein
target protein
adsorbent
flow
hydrophobic
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AU2004285928A
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English (en)
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AU2004285928A1 (en
Inventor
Sanchayita Ghose
Abhinav A. Shukla
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Amgen Inc
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Amgen Inc
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Application filed by Amgen Inc filed Critical Amgen Inc
Publication of AU2004285928A1 publication Critical patent/AU2004285928A1/en
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/20—Partition-, reverse-phase or hydrophobic interaction chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/30—Non-immunoglobulin-derived peptide or protein having an immunoglobulin constant or Fc region, or a fragment thereof, attached thereto

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Analytical Chemistry (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
AU2004285928A 2003-10-24 2004-10-22 Process for purifying proteins in a hydrophobic interaction chromatography flow-through fraction Active AU2004285928B2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US51448603P 2003-10-24 2003-10-24
US60/514,486 2003-10-24
PCT/US2004/035007 WO2005042569A1 (en) 2003-10-24 2004-10-22 Process for purifying proteins in a hydrophobic interaction chromatography flow-through fraction

Publications (2)

Publication Number Publication Date
AU2004285928A1 AU2004285928A1 (en) 2005-05-12
AU2004285928B2 true AU2004285928B2 (en) 2012-02-02

Family

ID=34549339

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2004285928A Active AU2004285928B2 (en) 2003-10-24 2004-10-22 Process for purifying proteins in a hydrophobic interaction chromatography flow-through fraction

Country Status (8)

Country Link
US (1) US7427659B2 (es)
EP (1) EP1687328B1 (es)
AT (1) ATE462716T1 (es)
AU (1) AU2004285928B2 (es)
CA (1) CA2542951C (es)
DE (1) DE602004026343D1 (es)
ES (1) ES2342539T3 (es)
WO (1) WO2005042569A1 (es)

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HUE049797T2 (hu) 2005-03-11 2020-10-28 Wyeth Llc Gyenge megoszlási kromatográfiás eljárás
US20070015133A1 (en) * 2005-07-13 2007-01-18 Umc Utrecht Holding B.V. Method for detecting and/or removing protein and/or peptide comprising a cross-beta structure from an aqueous solution comprising a protein
US8114832B2 (en) 2005-07-13 2012-02-14 Crossbeta Biosciences B.V. Method for detecting and/or removing a protein comprising a cross-beta structure from a pharmaceutical composition
EP2386861A3 (en) 2005-07-13 2012-07-18 Crossbeta Biosciences B.V. Cross-ß structure binding compounds
US7566401B2 (en) * 2005-10-03 2009-07-28 Kelm Mark A Process for separating and isolating xanthines, individual polar protic monomers, and polar protic oligomers
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AR058568A1 (es) 2005-12-20 2008-02-13 Bristol Myers Squibb Co Metodos para producir una composicion con moleculas ctla4-ig a partir de un medio de cultivo
US9605035B2 (en) 2008-01-10 2017-03-28 Research Development Foundation Vaccines and diagnostics for the ehrlichioses
US8188242B2 (en) * 2008-04-08 2012-05-29 Bio-Rad Laboratories, Inc. Chromatography purification of antibodies
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EP2445924B2 (en) 2009-06-25 2023-12-13 Amgen Inc. Capture purification processes for proteins expressed in a non-mammalian system
RU2012122240A (ru) 2009-10-30 2013-12-10 Эбботт Лэборетриз Конструкции sorf и экспрессия нескольких генов
EP2652491B1 (en) 2010-12-15 2017-11-29 Baxalta GmbH Eluate collection using conductivity gradient
EP2702077A2 (en) 2011-04-27 2014-03-05 AbbVie Inc. Methods for controlling the galactosylation profile of recombinantly-expressed proteins
US9783570B2 (en) 2011-07-01 2017-10-10 Hoffmann-La Roche Inc. Method for separation of monomeric polypeptides from aggregated polypeptides
JP6280499B2 (ja) * 2011-07-08 2018-02-14 メルク・シャープ・アンド・ドーム・コーポレーションＭｅｒｃｋ Ｓｈａｒｐ ＆ Ｄｏｈｍｅ Ｃｏｒｐ． Ｆｃ融合タンパク質の精製方法
KR101454316B1 (ko) * 2011-08-17 2014-10-27 한화케미칼 주식회사 활성형 ＴＮＦＲ-Ｆｃ 융합 단백질을 제조하는 방법
SG10201701224UA (en) 2012-03-12 2017-04-27 Merck Patent Gmbh Removal of protein aggregates from biopharmaceutical preparations in a flowthrough mode
US9334319B2 (en) 2012-04-20 2016-05-10 Abbvie Inc. Low acidic species compositions
US9067990B2 (en) 2013-03-14 2015-06-30 Abbvie, Inc. Protein purification using displacement chromatography
WO2013158273A1 (en) 2012-04-20 2013-10-24 Abbvie Inc. Methods to modulate c-terminal lysine variant distribution
US9505833B2 (en) 2012-04-20 2016-11-29 Abbvie Inc. Human antibodies that bind human TNF-alpha and methods of preparing the same
WO2013176754A1 (en) * 2012-05-24 2013-11-28 Abbvie Inc. Novel purification of antibodies using hydrophobic interaction chromatography
US9206390B2 (en) 2012-09-02 2015-12-08 Abbvie, Inc. Methods to control protein heterogeneity
US9512214B2 (en) 2012-09-02 2016-12-06 Abbvie, Inc. Methods to control protein heterogeneity
US10023608B1 (en) 2013-03-13 2018-07-17 Amgen Inc. Protein purification methods to remove impurities
WO2014151878A2 (en) 2013-03-14 2014-09-25 Abbvie Inc. Methods for modulating protein glycosylation profiles of recombinant protein therapeutics using monosaccharides and oligosacharides
US9017687B1 (en) 2013-10-18 2015-04-28 Abbvie, Inc. Low acidic species compositions and methods for producing and using the same using displacement chromatography
WO2014159579A1 (en) 2013-03-14 2014-10-02 Abbvie Inc. MUTATED ANTI-TNFα ANTIBODIES AND METHODS OF THEIR USE
PL2970378T3 (pl) * 2013-03-15 2021-12-06 Biogen Ma Inc. Chromatografia oddziaływań hydrofobowych białek w warunkach bez soli
EP3052640A2 (en) 2013-10-04 2016-08-10 AbbVie Inc. Use of metal ions for modulation of protein glycosylation profiles of recombinant proteins
US9181337B2 (en) 2013-10-18 2015-11-10 Abbvie, Inc. Modulated lysine variant species compositions and methods for producing and using the same
US8946395B1 (en) 2013-10-18 2015-02-03 Abbvie Inc. Purification of proteins using hydrophobic interaction chromatography
US9085618B2 (en) 2013-10-18 2015-07-21 Abbvie, Inc. Low acidic species compositions and methods for producing and using the same
US20150139988A1 (en) 2013-11-15 2015-05-21 Abbvie, Inc. Glycoengineered binding protein compositions
KR101891522B1 (ko) 2013-12-12 2018-08-24 이엠디 밀리포어 코포레이션 아크릴아미드 함유 필터를 사용한 단백질 분리
SG11201607341SA (en) * 2014-03-04 2016-10-28 Merck Patent Gmbh Robust antibody purification
TW201628649A (zh) 2014-10-09 2016-08-16 再生元醫藥公司 減少醫藥調配物中微可見顆粒之方法
MX2019000935A (es) 2016-08-16 2019-07-04 Regeneron Pharma Metodos para cuantificar anticuerpos individuales de una mezcla.
CN109923411B (zh) 2016-10-25 2022-05-31 里珍纳龙药品有限公司 用于色谱数据分析的方法和系统
JP7097433B2 (ja) 2017-08-17 2022-07-07 ジャスト－エヴォテック バイオロジックス、インコーポレイテッド 宿主細胞ガレクチンおよび他の夾雑物からグリコシル化タンパク質を精製する方法
TW202005694A (zh) 2018-07-02 2020-02-01 美商里珍納龍藥品有限公司 自混合物製備多肽之系統及方法
US20240083972A1 (en) 2020-12-22 2024-03-14 Amgen Inc. Cell culture method
WO2023215750A2 (en) 2022-05-02 2023-11-09 Regeneron Pharmaceuticals, Inc. Methods for reducing lipase activity

Citations (5)

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US5468847A (en) * 1994-03-10 1995-11-21 Minnesota Mining And Manufacturing Company Method of isolating and purifying a biomacromolecule
WO1996033208A1 (en) * 1995-04-20 1996-10-24 Genentech, Inc. Antibody purification by low-ph hydrophobic interaction chromatoggraphy
US6005082A (en) * 1994-11-14 1999-12-21 Genetics Institute, Inc. Process for purification of factor VIII
WO2000059927A1 (en) * 1999-04-01 2000-10-12 Cantab Pharmaceuticals Research Limited Purification of biological preparations
US20030009025A1 (en) * 1993-09-21 2003-01-09 Smith Leonard A. Recombinant vaccine against botulinum neurotoxin

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JPH0449299A (ja) 1990-06-14 1992-02-18 Tosoh Corp 免疫グロブリンフラグメントの精製法
US5463029A (en) * 1992-11-23 1995-10-31 Immunex Corporation Purification of fusion proteins comprising GM-CSF and IL-3
US5429746A (en) 1994-02-22 1995-07-04 Smith Kline Beecham Corporation Antibody purification
JP5025874B2 (ja) * 2000-03-27 2012-09-12 ジェネティクス インスティテュート，エルエルシー 高アニオン性タンパク質の精製方法
DE602004026343D1 (de) * 2003-10-24 2010-05-12 Amgen Inc Verfahren zur aufreinigung von proteinen in einer durchflussfraktion aus der chromatographie mit hydrophoben wechselwirkungen

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030009025A1 (en) * 1993-09-21 2003-01-09 Smith Leonard A. Recombinant vaccine against botulinum neurotoxin
US5468847A (en) * 1994-03-10 1995-11-21 Minnesota Mining And Manufacturing Company Method of isolating and purifying a biomacromolecule
US6005082A (en) * 1994-11-14 1999-12-21 Genetics Institute, Inc. Process for purification of factor VIII
WO1996033208A1 (en) * 1995-04-20 1996-10-24 Genentech, Inc. Antibody purification by low-ph hydrophobic interaction chromatoggraphy
WO2000059927A1 (en) * 1999-04-01 2000-10-12 Cantab Pharmaceuticals Research Limited Purification of biological preparations

Non-Patent Citations (2)

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Title
SCHOEL, BERND et al. 'Hydrophobic interaction chromatography for the purification of a mycobacterial heat shock protein of relative molecular mass 60 000.' Journal of Chromatography. 1991, Vol. 587, pages 19-23 *
SCHOEL, BERND et al. 'Hydrophobic interaction for the purification of cytolytic bacterial toxins.' Journal of Chromatography A. 1994, Vol. 667, pages 131-139 *

Also Published As

Publication number Publication date
CA2542951C (en) 2016-08-30
US20050136521A1 (en) 2005-06-23
ATE462716T1 (de) 2010-04-15
ES2342539T3 (es) 2010-07-08
WO2005042569A1 (en) 2005-05-12
CA2542951A1 (en) 2005-05-12
EP1687328A1 (en) 2006-08-09
US7427659B2 (en) 2008-09-23
DE602004026343D1 (de) 2010-05-12
EP1687328B1 (en) 2010-03-31
AU2004285928A1 (en) 2005-05-12
EP1687328A4 (en) 2006-11-15

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