AU2004242082B2 - URA5 gene and methods for stable genetic integration in yeast - Google Patents

URA5 gene and methods for stable genetic integration in yeast Download PDF

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Publication number
AU2004242082B2
AU2004242082B2 AU2004242082A AU2004242082A AU2004242082B2 AU 2004242082 B2 AU2004242082 B2 AU 2004242082B2 AU 2004242082 A AU2004242082 A AU 2004242082A AU 2004242082 A AU2004242082 A AU 2004242082A AU 2004242082 B2 AU2004242082 B2 AU 2004242082B2
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AU
Australia
Prior art keywords
nucleic acid
acid sequence
sequence
seq
gene
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Ceased
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AU2004242082A
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AU2004242082A1 (en
Inventor
Juergen H. Nett
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Glycofi Inc
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Glycofi Inc
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Publication of AU2004242082A1 publication Critical patent/AU2004242082A1/en
Application granted granted Critical
Publication of AU2004242082B2 publication Critical patent/AU2004242082B2/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/905—Stable introduction of foreign DNA into chromosome using homologous recombination in yeast
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1077—Pentosyltransferases (2.4.2)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Mycology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
AU2004242082A 2003-05-16 2004-04-30 URA5 gene and methods for stable genetic integration in yeast Ceased AU2004242082B2 (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US47143503P 2003-05-16 2003-05-16
US60/471,435 2003-05-16
US10/454,125 US7514253B2 (en) 2003-05-16 2003-06-03 URA5 gene and methods for stable genetic integration in yeast
US10/454,125 2003-06-03
PCT/US2004/013488 WO2004104165A2 (en) 2003-05-16 2004-04-30 Ura5 gene and methods for stable genetic integration in yeast

Publications (2)

Publication Number Publication Date
AU2004242082A1 AU2004242082A1 (en) 2004-12-02
AU2004242082B2 true AU2004242082B2 (en) 2008-12-11

Family

ID=33423332

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2004242082A Ceased AU2004242082B2 (en) 2003-05-16 2004-04-30 URA5 gene and methods for stable genetic integration in yeast

Country Status (6)

Country Link
US (5) US7514253B2 (enExample)
EP (2) EP2489740A1 (enExample)
JP (2) JP2007530007A (enExample)
AU (1) AU2004242082B2 (enExample)
CA (1) CA2525954A1 (enExample)
WO (1) WO2004104165A2 (enExample)

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7449308B2 (en) 2000-06-28 2008-11-11 Glycofi, Inc. Combinatorial DNA library for producing modified N-glycans in lower eukaryotes
US7598055B2 (en) * 2000-06-28 2009-10-06 Glycofi, Inc. N-acetylglucosaminyltransferase III expression in lower eukaryotes
US8697394B2 (en) * 2000-06-28 2014-04-15 Glycofi, Inc. Production of modified glycoproteins having multiple antennary structures
ATE440959T1 (de) * 2000-06-28 2009-09-15 Glycofi Inc Verfahren für die herstellung modifizierter glykoproteine
US7863020B2 (en) 2000-06-28 2011-01-04 Glycofi, Inc. Production of sialylated N-glycans in lower eukaryotes
US7332299B2 (en) 2003-02-20 2008-02-19 Glycofi, Inc. Endomannosidases in the modification of glycoproteins in eukaryotes
BRPI0711898A2 (pt) * 2006-05-19 2011-12-27 Glycofi Inc composiÇço de proteÍna da eritropoietina substancialmente homogÊnea, composiÇço farmacÊutica, e, mÉtodos de aumentar hematàcrito em um mamÍfero, e de produzir uma composiÇço de eritropoietina
JP5960947B2 (ja) * 2007-12-19 2016-08-02 グライコフィ， インコーポレイテッド タンパク質の製造のための酵母株
JP2011514157A (ja) * 2008-02-20 2011-05-06 グライコフィ， インコーポレイテッド 蛋白質生産用ベクター及び酵母株
US8067339B2 (en) * 2008-07-09 2011-11-29 Merck Sharp & Dohme Corp. Surface display of whole antibodies in eukaryotes
JP2010017131A (ja) * 2008-07-10 2010-01-28 Mitsui Eng & Shipbuild Co Ltd モーレラ属細菌及びプライマー
EP2324121B1 (en) 2008-08-12 2012-11-07 GlycoFi, Inc. Improved vectors and yeast strains for protein production: ca2+ atpase overexpression
EP2401378B1 (en) 2009-02-25 2013-08-14 Merck Sharp & Dohme Corp. Metabolic engineering of a galactose assimilation pathway in the glycoengineered yeast pichia pastoris
CN102648286A (zh) 2009-10-16 2012-08-22 默沙东公司 在巴斯德毕赤氏酵母中生产缺乏与针对宿主细胞抗原的抗体的可检测交叉结合活性的蛋白质的方法
KR101930961B1 (ko) 2010-02-24 2018-12-19 머크 샤프 앤드 돔 코포레이션 피키아 파스토리스에서 생산된 치료 당단백질 상의 ｎ-글리코실화 부위 점유를 증가시키는 방법
JP5733570B2 (ja) 2011-05-23 2015-06-10 ソニー株式会社 画像処理装置、画像処理方法、プログラム、および、記録媒体
US9707276B2 (en) 2012-12-03 2017-07-18 Merck Sharp & Dohme Corp. O-glycosylated carboxy terminal portion (CTP) peptide-based insulin and insulin analogues
US9944689B2 (en) 2013-03-07 2018-04-17 The General Hospital Corporation Human CTLA4 mutants and use thereof
US10597662B2 (en) 2013-11-22 2020-03-24 Jmtc Enzyme Corporation Transformant and process for production thereof, and process for production of lactic acid
JP6515502B2 (ja) * 2013-11-22 2019-05-22 Ａｇｃ株式会社 形質転換体の製造方法、形質転換体、および単座組込み用ベクターキット
JP6620375B2 (ja) 2014-10-10 2019-12-18 Ｊｍｔｃエンザイム株式会社 形質転換体およびその製造方法、ならびに乳酸の製造方法
WO2020172344A1 (en) 2019-02-19 2020-08-27 The Regents Of The University Of Colorado, A Body Corporate Bispecific immunotoxins targeting human cd25+ccr4+ tumors and regulatory t-cells
CN111850017A (zh) * 2019-04-30 2020-10-30 广州华真医药科技有限公司 一种基于ura3基因的表达载体及其构建方法
CN115838645B (zh) * 2022-09-15 2024-03-08 天津大学 一种高产乳清酸的酵母菌株及其应用
JP7797747B1 (ja) * 2024-11-20 2026-01-13 花王株式会社 新規遺伝子組換え用マーカー及びその利用

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH06506117A (ja) * 1991-04-01 1994-07-14 メルク　エンド　カンパニー　インコーポレーテッド ピキア（Ｐｉｃｈｉａ）蛋白質分解活性に影響する遺伝子およびその使用
DE19934408A1 (de) * 1999-07-22 2001-01-25 Consortium Elektrochem Ind pyrF Gen und seine Verwendung

Also Published As

Publication number Publication date
EP1633855A2 (en) 2006-03-15
US20090203105A1 (en) 2009-08-13
US20040229306A1 (en) 2004-11-18
JP2007530007A (ja) 2007-11-01
WO2004104165A3 (en) 2007-11-22
US20140051172A1 (en) 2014-02-20
US7514253B2 (en) 2009-04-07
US8524479B2 (en) 2013-09-03
JP2011115182A (ja) 2011-06-16
WO2004104165A2 (en) 2004-12-02
US20120116058A1 (en) 2012-05-10
EP2489740A1 (en) 2012-08-22
US8062879B2 (en) 2011-11-22
EP1633855A4 (en) 2008-08-20
AU2004242082A1 (en) 2004-12-02
US20150087013A1 (en) 2015-03-26
CA2525954A1 (en) 2004-12-02
WO2004104165A9 (en) 2006-03-02

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