AU2001290867A1 - Microfluidic devices and methods for performing temperature mediated reactions - Google Patents

Microfluidic devices and methods for performing temperature mediated reactions

Info

Publication number
AU2001290867A1
AU2001290867A1 AU2001290867A AU9086701A AU2001290867A1 AU 2001290867 A1 AU2001290867 A1 AU 2001290867A1 AU 2001290867 A AU2001290867 A AU 2001290867A AU 9086701 A AU9086701 A AU 9086701A AU 2001290867 A1 AU2001290867 A1 AU 2001290867A1
Authority
AU
Australia
Prior art keywords
reaction
temperature mediated
reaction channel
channel
methods
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2001290867A
Other languages
English (en)
Inventor
Seth R. Stern
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Caliper Life Sciences Inc
Original Assignee
Caliper Technologies Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Caliper Technologies Corp filed Critical Caliper Technologies Corp
Publication of AU2001290867A1 publication Critical patent/AU2001290867A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00Heating or cooling apparatus; Heat insulating devices
    • B01L7/52Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/50273Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means or forces applied to move the fluids
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06Fluid handling related problems
    • B01L2200/0605Metering of fluids
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/60Detection means characterised by use of a special device
    • C12Q2565/629Detection means characterised by use of a special device being a microfluidic device
AU2001290867A 2000-09-14 2001-09-13 Microfluidic devices and methods for performing temperature mediated reactions Abandoned AU2001290867A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US23234900P 2000-09-14 2000-09-14
US60232349 2000-09-14
PCT/US2001/028646 WO2002022878A1 (en) 2000-09-14 2001-09-13 Microfluidic devices and methods for performing temperature mediated reactions

Publications (1)

Publication Number Publication Date
AU2001290867A1 true AU2001290867A1 (en) 2002-03-26

Family

ID=22872752

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2001290867A Abandoned AU2001290867A1 (en) 2000-09-14 2001-09-13 Microfluidic devices and methods for performing temperature mediated reactions

Country Status (7)

Country Link
US (2) US6670153B2 (de)
EP (1) EP1317569B1 (de)
JP (1) JP4949600B2 (de)
AT (1) ATE448875T1 (de)
AU (1) AU2001290867A1 (de)
DE (1) DE60140553D1 (de)
WO (1) WO2002022878A1 (de)

Families Citing this family (142)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6048734A (en) 1995-09-15 2000-04-11 The Regents Of The University Of Michigan Thermal microvalves in a fluid flow method
US8900811B2 (en) 2000-11-16 2014-12-02 Caliper Life Sciences, Inc. Method and apparatus for generating thermal melting curves in a microfluidic device
US6692700B2 (en) 2001-02-14 2004-02-17 Handylab, Inc. Heat-reduction methods and systems related to microfluidic devices
US7829025B2 (en) 2001-03-28 2010-11-09 Venture Lending & Leasing Iv, Inc. Systems and methods for thermal actuation of microfluidic devices
US7010391B2 (en) 2001-03-28 2006-03-07 Handylab, Inc. Methods and systems for control of microfluidic devices
US6852287B2 (en) 2001-09-12 2005-02-08 Handylab, Inc. Microfluidic devices having a reduced number of input and output connections
US7323140B2 (en) 2001-03-28 2008-01-29 Handylab, Inc. Moving microdroplets in a microfluidic device
US8895311B1 (en) 2001-03-28 2014-11-25 Handylab, Inc. Methods and systems for control of general purpose microfluidic devices
US7440684B2 (en) 2001-04-12 2008-10-21 Spaid Michael A Method and apparatus for improved temperature control in microfluidic devices
EP1532275A4 (de) * 2002-07-26 2005-09-14 Applera Corp Biochemische mg-vermittelte warmstart-reaktionen
KR100959101B1 (ko) * 2003-02-20 2010-05-25 삼성전자주식회사 Pcr 반응기 및 pcr 반응기의 입구와 출구의 개폐를조절하는 방법
US7041481B2 (en) 2003-03-14 2006-05-09 The Regents Of The University Of California Chemical amplification based on fluid partitioning
EP2266687A3 (de) 2003-04-10 2011-06-29 The President and Fellows of Harvard College Erzeugung und Kontrolle von Fluiden
DE10318139B4 (de) * 2003-04-18 2005-02-17 PRO DESIGN Gesellschaft für Produktentwicklung mbH Selbstkonfigurerbarer Biochip
US7038472B1 (en) 2003-05-12 2006-05-02 Caliper Life Sciences, Inc. Methods and systems for measuring internal dimensions of microscale structures
JP4996248B2 (ja) 2003-07-31 2012-08-08 ハンディーラブ インコーポレイテッド 粒子含有サンプルの処理
CN104069784B (zh) 2003-08-27 2017-01-11 哈佛大学 流体物种的电子控制
CA2536360C (en) 2003-08-28 2013-08-06 Celula, Inc. Methods and apparatus for sorting cells using an optical switch in a microfluidic channel network
US9597644B2 (en) 2003-09-05 2017-03-21 Stokes Bio Limited Methods for culturing and analyzing cells
EP1663497B2 (de) 2003-09-05 2020-03-25 Stokes Bio Limited Mikrofluidanalysesystem
DE10353985A1 (de) * 2003-11-19 2005-06-23 Olympus Biosystems Gmbh Einrichtung und Verfahren zum Manipulieren und Analysieren von Mikroobjekten auf Grundlage eines zumindest bereichsweise als fluidisches Mikrosystem oder/und Chipsystem ausgeführten Mikrosystems
JP4592060B2 (ja) * 2004-04-26 2010-12-01 キヤノン株式会社 Pcr増幅反応装置、ならびに、該装置を利用するpcr増幅反応方法
AU2005241080B2 (en) 2004-05-03 2011-08-11 Handylab, Inc. Processing polynucleotide-containing samples
US8852862B2 (en) 2004-05-03 2014-10-07 Handylab, Inc. Method for processing polynucleotide-containing samples
US20060030037A1 (en) 2004-05-28 2006-02-09 Victor Joseph Thermo-controllable high-density chips for multiplex analyses
JP4990771B2 (ja) 2004-07-28 2012-08-01 キヤノン ユー.エス. ライフ サイエンシズ, インコーポレイテッド 微生物のゲノムdnaをモニターする方法
US7604938B2 (en) * 2005-02-18 2009-10-20 Canon U.S. Life Sciences, Inc. Devices and methods for monitoring genomic DNA of organisms
EP1890803B1 (de) * 2005-06-15 2010-07-21 Rhodia Opérations Mikrofluidikzirkulationsvorrichtung zur bestimmung von parametern einer physikalischen und/oder chemischen transformation und verwendung davon
EP1754536B1 (de) * 2005-08-16 2008-12-24 Agilent Technologies, Inc. Flüssigkeitseinspritzsystem
CN101300352B (zh) 2005-09-01 2013-03-20 佳能美国生命科学公司 检测、分析和鉴定基因组dna的方法和分子诊断装置
US20090155894A1 (en) * 2005-10-17 2009-06-18 Soper Steven A Electrokinetic Thermal Cycler and Reactor
WO2007091228A1 (en) 2006-02-07 2007-08-16 Stokes Bio Limited A liquid bridge and system
ES2393758T3 (es) 2006-03-15 2012-12-27 Micronics, Inc. Ensayos integrados de ácidos nucleicos
US8088616B2 (en) 2006-03-24 2012-01-03 Handylab, Inc. Heater unit for microfluidic diagnostic system
US8883490B2 (en) 2006-03-24 2014-11-11 Handylab, Inc. Fluorescence detector for microfluidic diagnostic system
US11806718B2 (en) 2006-03-24 2023-11-07 Handylab, Inc. Fluorescence detector for microfluidic diagnostic system
DK2001990T3 (en) 2006-03-24 2016-10-03 Handylab Inc Integrated microfluidic sample processing system and method for its use
US10900066B2 (en) 2006-03-24 2021-01-26 Handylab, Inc. Microfluidic system for amplifying and detecting polynucleotides in parallel
US7998708B2 (en) 2006-03-24 2011-08-16 Handylab, Inc. Microfluidic system for amplifying and detecting polynucleotides in parallel
US7846314B2 (en) * 2006-05-11 2010-12-07 Agilent Technologies , Inc Handling a plurality of samples
US7629124B2 (en) * 2006-06-30 2009-12-08 Canon U.S. Life Sciences, Inc. Real-time PCR in micro-channels
US8709787B2 (en) 2006-11-14 2014-04-29 Handylab, Inc. Microfluidic cartridge and method of using same
US8252581B2 (en) 2007-01-22 2012-08-28 Wafergen, Inc. Apparatus for high throughput chemical reactions
US8691164B2 (en) * 2007-04-20 2014-04-08 Celula, Inc. Cell sorting system and methods
US20080280466A1 (en) * 2007-05-08 2008-11-13 Imation Corp. USB memory device
BRPI0721675A2 (pt) * 2007-05-18 2013-01-22 Medimate Holding B V dispositivo de manuseio de amostra de mediÇço, tomada conectÁvel a um aparelho de avaliaÇço de mediÇço, dispositivo de avaliaÇço de mediÇço, mÉtodo para avaliaÇço de pelo menos um parÂmetro de uma amostra lÍquida, mÉtodo para a montagem de um dispositivo de manuseio de amostra de mediÇço
US8287820B2 (en) 2007-07-13 2012-10-16 Handylab, Inc. Automated pipetting apparatus having a combined liquid pump and pipette head system
US9618139B2 (en) 2007-07-13 2017-04-11 Handylab, Inc. Integrated heater and magnetic separator
US8133671B2 (en) 2007-07-13 2012-03-13 Handylab, Inc. Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples
US8182763B2 (en) 2007-07-13 2012-05-22 Handylab, Inc. Rack for sample tubes and reagent holders
USD621060S1 (en) 2008-07-14 2010-08-03 Handylab, Inc. Microfluidic cartridge
US8105783B2 (en) 2007-07-13 2012-01-31 Handylab, Inc. Microfluidic cartridge
US8324372B2 (en) 2007-07-13 2012-12-04 Handylab, Inc. Polynucleotide capture materials, and methods of using same
US20090136385A1 (en) 2007-07-13 2009-05-28 Handylab, Inc. Reagent Tube
US9186677B2 (en) 2007-07-13 2015-11-17 Handylab, Inc. Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples
US8016260B2 (en) 2007-07-19 2011-09-13 Formulatrix, Inc. Metering assembly and method of dispensing fluid
CN104307581B (zh) * 2007-08-09 2017-04-12 诺思可有限公司 关联的多参数单细胞测定及残余生物材料回收的方法和装置
JP5523327B2 (ja) 2007-10-12 2014-06-18 レオニックス,インコーポレイテッド 統合型マイクロ流体デバイスおよび方法
US9114397B2 (en) * 2007-10-25 2015-08-25 Canon U.S. Life Sciences, Inc. Method of reducing cross-contamination in continuous amplification reactions in a channel
US9393566B2 (en) 2008-06-23 2016-07-19 Canon U.S. Life Sciences, Inc. System and method for temperature referencing for melt curve data collection
USD618820S1 (en) 2008-07-11 2010-06-29 Handylab, Inc. Reagent holder
USD787087S1 (en) 2008-07-14 2017-05-16 Handylab, Inc. Housing
EP2153901A1 (de) * 2008-08-01 2010-02-17 Eppendorf Ag Temperierungsvorrichtung mit Testmöglichkeit und Verfahren zum Testen einer Temperierungsvorrichtung
US8322918B2 (en) * 2008-08-01 2012-12-04 Eppendorf Ag Tempering apparatus with testing device and method for testing a tempering apparatus
US9492797B2 (en) 2008-09-23 2016-11-15 Bio-Rad Laboratories, Inc. System for detection of spaced droplets
US11130128B2 (en) 2008-09-23 2021-09-28 Bio-Rad Laboratories, Inc. Detection method for a target nucleic acid
US9156010B2 (en) 2008-09-23 2015-10-13 Bio-Rad Laboratories, Inc. Droplet-based assay system
US9598725B2 (en) 2010-03-02 2017-03-21 Bio-Rad Laboratories, Inc. Emulsion chemistry for encapsulated droplets
US8709762B2 (en) 2010-03-02 2014-04-29 Bio-Rad Laboratories, Inc. System for hot-start amplification via a multiple emulsion
US9132394B2 (en) 2008-09-23 2015-09-15 Bio-Rad Laboratories, Inc. System for detection of spaced droplets
US9417190B2 (en) 2008-09-23 2016-08-16 Bio-Rad Laboratories, Inc. Calibrations and controls for droplet-based assays
WO2011120020A1 (en) 2010-03-25 2011-09-29 Quantalife, Inc. Droplet transport system for detection
US8633015B2 (en) 2008-09-23 2014-01-21 Bio-Rad Laboratories, Inc. Flow-based thermocycling system with thermoelectric cooler
US9764322B2 (en) 2008-09-23 2017-09-19 Bio-Rad Laboratories, Inc. System for generating droplets with pressure monitoring
US10512910B2 (en) 2008-09-23 2019-12-24 Bio-Rad Laboratories, Inc. Droplet-based analysis method
US8951939B2 (en) 2011-07-12 2015-02-10 Bio-Rad Laboratories, Inc. Digital assays with multiplexed detection of two or more targets in the same optical channel
US8100293B2 (en) 2009-01-23 2012-01-24 Formulatrix, Inc. Microfluidic dispensing assembly
WO2010141131A1 (en) 2009-06-04 2010-12-09 Lockheed Martin Corporation Multiple-sample microfluidic chip for dna analysis
EP2940153B1 (de) 2009-09-02 2020-05-13 Bio-Rad Laboratories, Inc. System zum Mischen von Flüssigkeiten durch Koaleszenz mehrerer Emulsionen
KR101851117B1 (ko) 2010-01-29 2018-04-23 마이크로닉스 인코포레이티드. 샘플-투-앤서 마이크로유체 카트리지
CA2767182C (en) 2010-03-25 2020-03-24 Bio-Rad Laboratories, Inc. Droplet generation for droplet-based assays
EP2550351A4 (de) 2010-03-25 2014-07-09 Quantalife Inc Detektionssystem für assays auf tröpfchenbasis
AU2011315951B2 (en) 2010-10-15 2015-03-19 Lockheed Martin Corporation Micro fluidic optic design
EP2635840B1 (de) 2010-11-01 2017-01-04 Bio-Rad Laboratories, Inc. System zur formung von emulsionen
CA2830443C (en) 2011-03-18 2021-11-16 Bio-Rad Laboratories, Inc. Multiplexed digital assays with combinatorial use of signals
BR112013026451B1 (pt) 2011-04-15 2021-02-09 Becton, Dickinson And Company sistema e método para realizar ensaios de diagnóstico molecular em várias amostras em paralelo e simultaneamente amplificação em tempo real em pluralidade de câmaras de reação de amplificação
EP2702175B1 (de) 2011-04-25 2018-08-08 Bio-Rad Laboratories, Inc. Verfahren und zusammensetzungen zur nukleinsäureanalyse
EP2737089B1 (de) 2011-07-29 2017-09-06 Bio-rad Laboratories, Inc. Bibliothekscharakterisierung durch digitalen test
WO2013049706A1 (en) 2011-09-30 2013-04-04 Becton, Dickinson And Company Unitized reagent strip
USD692162S1 (en) 2011-09-30 2013-10-22 Becton, Dickinson And Company Single piece reagent holder
WO2013067202A1 (en) 2011-11-04 2013-05-10 Handylab, Inc. Polynucleotide sample preparation device
CN104204812B (zh) 2012-02-03 2018-01-05 贝克顿·迪金森公司 用于分子诊断测试分配和测试之间兼容性确定的外部文件
US9322054B2 (en) 2012-02-22 2016-04-26 Lockheed Martin Corporation Microfluidic cartridge
JP6216494B2 (ja) * 2012-03-30 2017-10-18 セイコーエプソン株式会社 熱サイクル装置及び熱サイクル装置の制御方法
JP5935985B2 (ja) * 2012-03-30 2016-06-15 セイコーエプソン株式会社 熱サイクル装置
JP2013208066A (ja) 2012-03-30 2013-10-10 Seiko Epson Corp 熱サイクル装置及び熱サイクル装置の制御方法
JP5971463B2 (ja) 2012-03-30 2016-08-17 セイコーエプソン株式会社 熱サイクル装置及び熱サイクル装置の制御方法
WO2013155531A2 (en) 2012-04-13 2013-10-17 Bio-Rad Laboratories, Inc. Sample holder with a well having a wicking promoter
US10273541B2 (en) 2012-08-14 2019-04-30 10X Genomics, Inc. Methods and systems for processing polynucleotides
US11591637B2 (en) 2012-08-14 2023-02-28 10X Genomics, Inc. Compositions and methods for sample processing
MX364957B (es) 2012-08-14 2019-05-15 10X Genomics Inc Composiciones y metodos para microcapsulas.
US9701998B2 (en) 2012-12-14 2017-07-11 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10221442B2 (en) 2012-08-14 2019-03-05 10X Genomics, Inc. Compositions and methods for sample processing
US9951386B2 (en) 2014-06-26 2018-04-24 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10752949B2 (en) 2012-08-14 2020-08-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10323279B2 (en) 2012-08-14 2019-06-18 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10400280B2 (en) 2012-08-14 2019-09-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
US20150376609A1 (en) 2014-06-26 2015-12-31 10X Genomics, Inc. Methods of Analyzing Nucleic Acids from Individual Cells or Cell Populations
US10533221B2 (en) 2012-12-14 2020-01-14 10X Genomics, Inc. Methods and systems for processing polynucleotides
CA2894694C (en) 2012-12-14 2023-04-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10518262B2 (en) 2012-12-21 2019-12-31 Perkinelmer Health Sciences, Inc. Low elasticity films for microfluidic use
JP6498125B2 (ja) 2012-12-21 2019-04-10 マイクロニクス, インコーポレイテッド 流体回路および関連する製造方法
JP2016509206A (ja) 2012-12-21 2016-03-24 マイクロニクス, インコーポレイテッド 携帯型蛍光検出システムおよびマイクロアッセイカートリッジ
CA2900481A1 (en) 2013-02-08 2014-08-14 10X Genomics, Inc. Polynucleotide barcode generation
EP2962092A4 (de) * 2013-03-01 2016-08-24 Wave 80 Biosciences Inc Verfahren und systeme für eine verbesserte mikrofluidische verarbeitung
US9995412B2 (en) 2013-03-01 2018-06-12 Wave 80 Biosciences, Inc. Long-throw microfluidic actuator
EP2994543B1 (de) 2013-05-07 2018-08-15 Micronics, Inc. Vorrichtung zur herstellung und analyse von nukleinsäuren
WO2014182844A1 (en) 2013-05-07 2014-11-13 Micronics, Inc. Microfluidic devices and methods for performing serum separation and blood cross-matching
WO2014182831A1 (en) 2013-05-07 2014-11-13 Micronics, Inc. Methods for preparation of nucleic acid-containing samples using clay minerals and alkaline solutions
JP2015154722A (ja) 2014-02-20 2015-08-27 セイコーエプソン株式会社 核酸増幅反応容器及び核酸増幅反応装置
AU2015243445B2 (en) 2014-04-10 2020-05-28 10X Genomics, Inc. Fluidic devices, systems, and methods for encapsulating and partitioning reagents, and applications of same
US20160122817A1 (en) 2014-10-29 2016-05-05 10X Genomics, Inc. Methods and compositions for targeted nucleic acid sequencing
US9975122B2 (en) 2014-11-05 2018-05-22 10X Genomics, Inc. Instrument systems for integrated sample processing
SG11201705615UA (en) 2015-01-12 2017-08-30 10X Genomics Inc Processes and systems for preparing nucleic acid sequencing libraries and libraries prepared using same
DK3259602T3 (da) 2015-02-20 2021-02-15 Takara Bio Usa Inc Fremgangsmåde til hurtig nøjagtig dispensering, visualisering og analyse af enkeltceller
EP3262407B1 (de) 2015-02-24 2023-08-30 10X Genomics, Inc. Partitionsverarbeitungsverfahren und -systeme
EP3262188B1 (de) 2015-02-24 2021-05-05 10X Genomics, Inc. Verfahren zur sequenzierung von nukleinsäuren in gesteuertem ausmass
SG11201804086VA (en) 2015-12-04 2018-06-28 10X Genomics Inc Methods and compositions for nucleic acid analysis
JP6206688B2 (ja) * 2016-05-11 2017-10-04 セイコーエプソン株式会社 熱サイクル装置
WO2017197338A1 (en) 2016-05-13 2017-11-16 10X Genomics, Inc. Microfluidic systems and methods of use
JP7387430B2 (ja) 2016-06-27 2023-11-28 ゾエティス サービシズ リミテッド ライアビリティ カンパニー 改変された導管を有する装置
WO2018017892A1 (en) 2016-07-21 2018-01-25 Takara Bio Usa, Inc. Multi-z imaging and dispensing with multi-well devices
US10550429B2 (en) 2016-12-22 2020-02-04 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10011872B1 (en) 2016-12-22 2018-07-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10815525B2 (en) 2016-12-22 2020-10-27 10X Genomics, Inc. Methods and systems for processing polynucleotides
EP4029939B1 (de) 2017-01-30 2023-06-28 10X Genomics, Inc. Verfahren und systeme für eine einzelzellbarcodierung auf tröpfchenbasis
CA3057501C (en) * 2017-04-21 2021-10-26 Abaxis, Inc. Systems, devices and methods for microfluidic analysis
CN116064732A (zh) 2017-05-26 2023-05-05 10X基因组学有限公司 转座酶可接近性染色质的单细胞分析
US10400235B2 (en) 2017-05-26 2019-09-03 10X Genomics, Inc. Single cell analysis of transposase accessible chromatin
SG11201913654QA (en) 2017-11-15 2020-01-30 10X Genomics Inc Functionalized gel beads
US10829815B2 (en) 2017-11-17 2020-11-10 10X Genomics, Inc. Methods and systems for associating physical and genetic properties of biological particles
SG11202009889VA (en) 2018-04-06 2020-11-27 10X Genomics Inc Systems and methods for quality control in single cell processing

Family Cites Families (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8917963D0 (en) * 1989-08-05 1989-09-20 Scras Apparatus for repeated automatic execution of a thermal cycle for treatment of biological samples
US5639423A (en) 1992-08-31 1997-06-17 The Regents Of The University Of Calfornia Microfabricated reactor
US6001229A (en) 1994-08-01 1999-12-14 Lockheed Martin Energy Systems, Inc. Apparatus and method for performing microfluidic manipulations for chemical analysis
US5856174A (en) 1995-06-29 1999-01-05 Affymetrix, Inc. Integrated nucleic acid diagnostic device
US5863502A (en) 1996-01-24 1999-01-26 Sarnoff Corporation Parallel reaction cassette and associated devices
US5942443A (en) 1996-06-28 1999-08-24 Caliper Technologies Corporation High throughput screening assay systems in microscale fluidic devices
CA2253587C (en) 1996-05-09 2008-01-29 3-Dimensional Pharmaceuticals, Inc. Microplate thermal shift assay and apparatus for ligand development and multi-variable protein chemistry optimization
US5779868A (en) 1996-06-28 1998-07-14 Caliper Technologies Corporation Electropipettor and compensation means for electrophoretic bias
NZ333346A (en) * 1996-06-28 2000-03-27 Caliper Techn Corp High-throughput screening assay systems in microscale fluidic devices
US5800690A (en) 1996-07-03 1998-09-01 Caliper Technologies Corporation Variable control of electroosmotic and/or electrophoretic forces within a fluid-containing structure via electrical forces
JP2001521622A (ja) 1997-04-04 2001-11-06 カリパー テクノロジーズ コーポレイション 閉ループ生化学分析器
AU746098B2 (en) * 1997-09-02 2002-04-18 Caliper Life Sciences, Inc. Microfluidic system with electrofluidic and electrothermal controls
US5965410A (en) * 1997-09-02 1999-10-12 Caliper Technologies Corp. Electrical current for controlling fluid parameters in microchannels
WO1999018438A1 (en) * 1997-10-02 1999-04-15 Aclara Biosciences, Inc. Capillary assays involving separation of free and bound species
US6202141B1 (en) 1998-06-16 2001-03-13 International Business Machines Corporation Method and apparatus for performing vector operation using separate multiplication on odd and even data elements of source vectors
US6062261A (en) * 1998-12-16 2000-05-16 Lockheed Martin Energy Research Corporation MicrofluIdic circuit designs for performing electrokinetic manipulations that reduce the number of voltage sources and fluid reservoirs
US6150119A (en) * 1999-01-19 2000-11-21 Caliper Technologies Corp. Optimized high-throughput analytical system
US6300075B1 (en) 1999-02-03 2001-10-09 Ortho-Clinical Diagnostics, Inc Enhancement of the specificity of nucleic acid amplification by carrier nucleic acid
US6303343B1 (en) 1999-04-06 2001-10-16 Caliper Technologies Corp. Inefficient fast PCR
US6221600B1 (en) * 1999-10-08 2001-04-24 Board Of Regents, The University Of Texas System Combinatorial oligonucleotide PCR: a method for rapid, global expression analysis

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DE60140553D1 (de) 2009-12-31
ATE448875T1 (de) 2009-12-15
US6670153B2 (en) 2003-12-30
JP4949600B2 (ja) 2012-06-13
JP2004508837A (ja) 2004-03-25
US20040081997A1 (en) 2004-04-29
EP1317569B1 (de) 2009-11-18
EP1317569A1 (de) 2003-06-11
WO2002022878A1 (en) 2002-03-21
US7153673B2 (en) 2006-12-26
US20020119536A1 (en) 2002-08-29

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