ATE532856T1 - Modifizierte polymerasen des typs b zum verbesserten einbau von nukleotidanaloga - Google Patents

Modifizierte polymerasen des typs b zum verbesserten einbau von nukleotidanaloga

Info

Publication number
ATE532856T1
ATE532856T1 AT04768438T AT04768438T ATE532856T1 AT E532856 T1 ATE532856 T1 AT E532856T1 AT 04768438 T AT04768438 T AT 04768438T AT 04768438 T AT04768438 T AT 04768438T AT E532856 T1 ATE532856 T1 AT E532856T1
Authority
AT
Austria
Prior art keywords
polymerases
nucleotide analogs
improved incorporation
modified type
incorporation
Prior art date
Application number
AT04768438T
Other languages
English (en)
Inventor
Geoffrey Paul Smith
David Mark Dunstan Bailey
Raquel Maria Sanches
Harold Swerdlow
David James Earnshaw
Original Assignee
Illumina Cambridge Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=29226911&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=ATE532856(T1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Illumina Cambridge Ltd filed Critical Illumina Cambridge Ltd
Application granted granted Critical
Publication of ATE532856T1 publication Critical patent/ATE532856T1/de

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07007—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AT04768438T 2003-09-11 2004-09-10 Modifizierte polymerasen des typs b zum verbesserten einbau von nukleotidanaloga ATE532856T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB0321306.3A GB0321306D0 (en) 2003-09-11 2003-09-11 Modified polymerases for improved incorporation of nucleotide analogues
PCT/GB2004/003891 WO2005024010A1 (en) 2003-09-11 2004-09-10 Modified polymerases for improved incorporation of nucleotide analogues

Publications (1)

Publication Number Publication Date
ATE532856T1 true ATE532856T1 (de) 2011-11-15

Family

ID=29226911

Family Applications (2)

Application Number Title Priority Date Filing Date
AT10180445T ATE557084T1 (de) 2003-09-11 2004-09-10 Modifizierte dna-polymerasen zur verbesserten inkorporation von nukleotidanaloga
AT04768438T ATE532856T1 (de) 2003-09-11 2004-09-10 Modifizierte polymerasen des typs b zum verbesserten einbau von nukleotidanaloga

Family Applications Before (1)

Application Number Title Priority Date Filing Date
AT10180445T ATE557084T1 (de) 2003-09-11 2004-09-10 Modifizierte dna-polymerasen zur verbesserten inkorporation von nukleotidanaloga

Country Status (7)

Country Link
US (7) US8460910B2 (de)
EP (2) EP1664287B1 (de)
JP (1) JP5706057B2 (de)
AT (2) ATE557084T1 (de)
DK (1) DK2325304T3 (de)
GB (1) GB0321306D0 (de)
WO (1) WO2005024010A1 (de)

Families Citing this family (453)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9708358B2 (en) 2000-10-06 2017-07-18 The Trustees Of Columbia University In The City Of New York Massive parallel method for decoding DNA and RNA
DE60127162T2 (de) 2000-10-06 2008-04-24 The Trustees Of Columbia University In The City Of New York Massives Parallelverfahren zur Dekodierung von DNA und RNA
US7057026B2 (en) 2001-12-04 2006-06-06 Solexa Limited Labelled nucleotides
GB0129012D0 (en) 2001-12-04 2002-01-23 Solexa Ltd Labelled nucleotides
US7414116B2 (en) 2002-08-23 2008-08-19 Illumina Cambridge Limited Labelled nucleotides
DK3002289T3 (en) 2002-08-23 2018-04-23 Illumina Cambridge Ltd MODIFIED NUCLEOTIDES FOR POLYNUCLEOTIDE SEQUENCE
US11008359B2 (en) 2002-08-23 2021-05-18 Illumina Cambridge Limited Labelled nucleotides
GB0321306D0 (en) 2003-09-11 2003-10-15 Solexa Ltd Modified polymerases for improved incorporation of nucleotide analogues
CA2557818A1 (en) * 2004-03-03 2005-09-15 The Trustees Of Columbia University In The City Of New York Photocleavable fluorescent nucleotides for dna sequencing on chip constructed by site-specific coupling chemistry
US20070048748A1 (en) * 2004-09-24 2007-03-01 Li-Cor, Inc. Mutant polymerases for sequencing and genotyping
EP1888743B1 (de) 2005-05-10 2011-08-03 Illumina Cambridge Limited Verbesserte polymerasen
GB0509508D0 (en) * 2005-05-10 2005-06-15 Solexa Ltd Improved polymerases
US9169510B2 (en) * 2005-06-21 2015-10-27 The Trustees Of Columbia University In The City Of New York Pyrosequencing methods and related compositions
WO2007053719A2 (en) * 2005-10-31 2007-05-10 The Trustees Of Columbia University In The City Of New York Chemically cleavable 3'-o-allyl-dntp-allyl-fluorophore fluorescent nucleotide analogues and related methods
GB2446084B (en) * 2005-10-31 2011-03-02 Univ Columbia Synthesis of four color 3-o-allyl modified photocleavable fluorescent nucleotides and related methods
EP1957983A4 (de) * 2005-11-21 2010-03-24 Univ Columbia Digitale multiplex-immunwahrnehmung unter verwendung einer bibliothek von photospaltbaren massen-tags
EP3722409A1 (de) 2006-03-31 2020-10-14 Illumina, Inc. Systeme und vorrichtungen zur "sequence-by-synthesis"-analyse
WO2008029085A2 (en) * 2006-09-06 2008-03-13 Medical Research Council Polymerase
US20090305345A1 (en) 2006-10-23 2009-12-10 Medical Research Council Polymerase
KR100777230B1 (ko) * 2006-11-30 2007-11-28 한국해양연구원 써모코커스 유래 돌연변이 ｄｎａ 중합효소들 및 그의유전자들
US7883869B2 (en) 2006-12-01 2011-02-08 The Trustees Of Columbia University In The City Of New York Four-color DNA sequencing by synthesis using cleavable fluorescent nucleotide reversible terminators
US11035823B2 (en) 2009-03-17 2021-06-15 Qiagen Sciences, Llc Methods and devices for sequencing nucleic acids in smaller batches
US8481259B2 (en) 2007-02-05 2013-07-09 Intelligent Bio-Systems, Inc. Methods and devices for sequencing nucleic acids in smaller batches
US11940413B2 (en) 2007-02-05 2024-03-26 IsoPlexis Corporation Methods and devices for sequencing nucleic acids in smaller batches
EP2940029B1 (de) 2007-10-19 2023-11-29 The Trustees of Columbia University in the City of New York Design und synthese von spaltbaren fluoreszierenden nukleotiden als reversible terminatoren zur dna-sequenzierung durch synthese
EP2209911B1 (de) 2007-10-19 2013-10-16 The Trustees of Columbia University in the City of New York Dna-sequenzierung mit reversiblen nicht-fluoreszenz-nukleotid-terminatoren und mit einer spaltbaren markierung modifizierten nukleotidterminatoren und eine deoxyinosineverbindung mit einer reversiblen terminatorgruppe
WO2009097368A2 (en) 2008-01-28 2009-08-06 Complete Genomics, Inc. Methods and compositions for efficient base calling in sequencing reactions
US9017973B2 (en) * 2008-03-19 2015-04-28 Intelligent Biosystems, Inc. Methods and compositions for incorporating nucleotides
US8883999B2 (en) * 2008-03-19 2014-11-11 Intelligent Bio Systems, Inc. Methods and solutions for inhibiting undesired cleaving of labels
US20110045489A1 (en) * 2008-04-22 2011-02-24 New England Biolabs, Inc. Polymerases for Incorporating Modified Nucleotides
US8039817B2 (en) 2008-05-05 2011-10-18 Illumina, Inc. Compensator for multiple surface imaging
US20110105356A1 (en) * 2008-05-07 2011-05-05 Derosier Chad F Compositions and methods for providing substances to and from an array
EP2358913B1 (de) 2008-11-03 2017-03-22 The Regents of The University of California Nachweisverfahren für modifikationsresistente nukleinsäuren
US20100151473A1 (en) * 2008-12-10 2010-06-17 Yeakley Joanne M Methods and compositions for hybridizing nucleic acids
EP2379748A4 (de) 2008-12-23 2012-08-29 Illumina Inc Multibase-freisetzung für lange auslesevorgänge bei squenzierungen mittels syntheseprotokollen
US20100221726A1 (en) 2009-02-09 2010-09-02 Frederic Zenhausern Relating to devices
US20100279882A1 (en) * 2009-05-01 2010-11-04 Mostafa Ronaghi Sequencing methods
US20120202276A1 (en) * 2010-02-26 2012-08-09 Life Technologies Corporation Modified Proteins and Methods of Making and Using Same
WO2011106629A2 (en) * 2010-02-26 2011-09-01 Life Technologies Corporation Modified proteins and methods of making and using same
WO2011123246A2 (en) 2010-04-01 2011-10-06 Illumina, Inc. Solid-phase clonal amplification and related methods
US10787701B2 (en) 2010-04-05 2020-09-29 Prognosys Biosciences, Inc. Spatially encoded biological assays
US9371598B2 (en) 2010-04-05 2016-06-21 Prognosys Biosciences, Inc. Spatially encoded biological assays
US20190300945A1 (en) 2010-04-05 2019-10-03 Prognosys Biosciences, Inc. Spatially Encoded Biological Assays
GB201007384D0 (en) * 2010-04-30 2010-06-16 Medical Res Council Enzymes
US8993737B2 (en) 2010-08-25 2015-03-31 Pacific Biosciences, Inc. Phospholinked dye analogs with an amino acid linker
US9029103B2 (en) 2010-08-27 2015-05-12 Illumina Cambridge Limited Methods for sequencing polynucleotides
US8483969B2 (en) 2010-09-17 2013-07-09 Illuminia, Inc. Variation analysis for multiple templates on a solid support
US8759038B2 (en) 2010-09-29 2014-06-24 Illumina Cambridge Limited Compositions and methods for sequencing nucleic acids
WO2012055929A1 (en) 2010-10-26 2012-05-03 Illumina, Inc. Sequencing methods
EP2632593B1 (de) 2010-10-27 2021-09-29 Illumina, Inc. Durchflusszellen zur biologischen oder chemischen analyse
US8575071B2 (en) 2010-11-03 2013-11-05 Illumina, Inc. Reducing adapter dimer formation
EP2635679B1 (de) 2010-11-05 2017-04-19 Illumina, Inc. Verknüpfung von sequenzstücken mit gepaarten code-tags
EP2643484A4 (de) 2010-11-22 2014-04-16 Univ California Verfahren zur identifizierung eines rna-transkripts zellulären ursprungs
GB201106254D0 (en) 2011-04-13 2011-05-25 Frisen Jonas Method and product
WO2013049135A1 (en) 2011-09-26 2013-04-04 Gen-Probe Incorporated Algorithms for sequence determinations
US10378051B2 (en) 2011-09-29 2019-08-13 Illumina Cambridge Limited Continuous extension and deblocking in reactions for nucleic acids synthesis and sequencing
EP2776165A2 (de) 2011-11-07 2014-09-17 Illumina, Inc. Integrierte sequenzierungsvorrichtungen und verwendungsverfahren
EP2788499B1 (de) 2011-12-09 2016-01-13 Illumina, Inc. Expandierte wurzel für polymer-tags
WO2013117595A2 (en) 2012-02-07 2013-08-15 Illumina Cambridge Limited Targeted enrichment and amplification of nucleic acids on a support
NO2694769T3 (de) 2012-03-06 2018-03-03
US9444880B2 (en) 2012-04-11 2016-09-13 Illumina, Inc. Cloud computing environment for biological data
WO2013173844A1 (en) 2012-05-18 2013-11-21 Pacific Biosciences Of California, Inc. Heteroarylcyanine dyes
US9315864B2 (en) 2012-05-18 2016-04-19 Pacific Biosciences Of California, Inc. Heteroarylcyanine dyes with sulfonic acid substituents
EP2870264A4 (de) 2012-07-03 2016-03-02 Sloan Kettering Inst Cancer Quantitative beurteilung der wiederherstellung eines menschlichen t-zellen-repertoires nach der transplantation allogener blutbildender stammzellen
NL2017959B1 (en) 2016-12-08 2018-06-19 Illumina Inc Cartridge assembly
JP6478445B2 (ja) * 2012-09-28 2019-03-06 東洋紡株式会社 改変された耐熱性ｄｎａポリメラーゼ
EP3511423B2 (de) 2012-10-17 2024-05-29 Spatial Transcriptomics AB Verfahren und produkt zur optimierung einer lokalisierten oder räumlichen detektion einer genexpression in einer gewebeprobe
US9116139B2 (en) 2012-11-05 2015-08-25 Illumina, Inc. Sequence scheduling and sample distribution techniques
WO2014107502A2 (en) * 2013-01-04 2014-07-10 Arizona Board Of Regents, A Body Corporate Of The State Of Arizona, Acting For And On Behalf Of Arizona State University Methods and compositions for replication of threose nucleic acids
US9805407B2 (en) 2013-01-25 2017-10-31 Illumina, Inc. Methods and systems for using a cloud computing environment to configure and sell a biological sample preparation cartridge and share related data
US9650406B2 (en) 2013-02-28 2017-05-16 Centrillion Technology Holdings Corporation Reversible terminator molecules and methods of their use
EP2964624B1 (de) 2013-03-08 2017-01-04 Illumina Cambridge Limited Rhodaminverbindungen und verwendung als fluoreszenzmarker
WO2014135221A1 (en) 2013-03-08 2014-09-12 Illumina Cambridge Ltd Polymethine compounds and their use as fluorescent labels
EP3919617A1 (de) 2013-03-13 2021-12-08 Illumina, Inc. Verfahren und zusammensetzungen zur nukleinsäuresequenzierung
EP2971070B2 (de) 2013-03-14 2021-03-03 Illumina, Inc. Modifizierte polymerasen zur verbesserten inkorporation von nukleotidanaloga
US9146248B2 (en) 2013-03-14 2015-09-29 Intelligent Bio-Systems, Inc. Apparatus and methods for purging flow cells in nucleic acid sequencing instruments
CA2903095C (en) 2013-03-15 2018-02-13 Xiaohai Liu Modified nucleosides or nucleotides
WO2014144883A1 (en) 2013-03-15 2014-09-18 The Trustees Of Columbia University In The City Of New York Raman cluster tagged molecules for biological imaging
US9591268B2 (en) 2013-03-15 2017-03-07 Qiagen Waltham, Inc. Flow cell alignment methods and systems
US9771613B2 (en) 2013-04-02 2017-09-26 Molecular Assemblies, Inc. Methods and apparatus for synthesizing nucleic acid
US9279149B2 (en) 2013-04-02 2016-03-08 Molecular Assemblies, Inc. Methods and apparatus for synthesizing nucleic acids
US11331643B2 (en) 2013-04-02 2022-05-17 Molecular Assemblies, Inc. Reusable initiators for synthesizing nucleic acids
US10683536B2 (en) 2013-04-02 2020-06-16 Molecular Assemblies, Inc. Reusable initiators for synthesizing nucleic acids
US8808989B1 (en) 2013-04-02 2014-08-19 Molecular Assemblies, Inc. Methods and apparatus for synthesizing nucleic acids
US11384377B2 (en) 2013-04-02 2022-07-12 Molecular Assemblies, Inc. Reusable initiators for synthesizing nucleic acids
LT3013983T (lt) 2013-06-25 2023-05-10 Prognosys Biosciences, Inc. Erdviniai koduoti biologiniai tyrimai, naudojant mikrofluidinį įrenginį
US9352315B2 (en) 2013-09-27 2016-05-31 Taiwan Semiconductor Manufacturing Company, Ltd. Method to produce chemical pattern in micro-fluidic structure
US10540783B2 (en) 2013-11-01 2020-01-21 Illumina, Inc. Image analysis useful for patterned objects
PT3077943T (pt) 2013-12-03 2020-08-21 Illumina Inc Métodos e sistemas para analisar dados de imagem
MX360883B (es) 2013-12-10 2018-11-21 Illumina Inc Biosensores para análisis biológico o químico y métodos para fabricarlos.
US10537889B2 (en) 2013-12-31 2020-01-21 Illumina, Inc. Addressable flow cell using patterned electrodes
WO2015123444A2 (en) 2014-02-13 2015-08-20 Illumina, Inc. Integrated consumer genomic services
MX379233B (es) 2014-02-18 2025-03-10 Illumina Inc Métodos y composiciones para el perfilado de adn.
US10767219B2 (en) 2014-03-11 2020-09-08 Illumina, Inc. Disposable, integrated microfluidic cartridge and methods of making and using same
GB201408077D0 (en) 2014-05-07 2014-06-18 Illumina Cambridge Ltd Polymethine compounds and their use as fluorescent labels
SG11201610168YA (en) 2014-05-16 2017-01-27 Illumina Inc Nucleic acid synthesis techniques
AU2015267189B2 (en) 2014-05-27 2019-12-05 Illumina, Inc. Systems and methods for biochemical analysis including a base instrument and a removable cartridge
US20150353989A1 (en) 2014-06-09 2015-12-10 Illumina Cambridge Limited Sample preparation for nucleic acid amplification
PL3161154T3 (pl) 2014-06-27 2020-10-19 Illumina, Inc. Zmodyfikowane polimerazy do ulepszonego włączania analogów nukleotydów
US9469862B2 (en) * 2014-08-19 2016-10-18 Arizona Board Of Regents On Behalf Of Arizona State University Modified polymerases for replication of threose nucleic acids
US9963687B2 (en) 2014-08-27 2018-05-08 New England Biolabs, Inc. Fusion polymerase and method for using the same
CN113584015B (zh) 2014-08-27 2025-05-02 新英格兰生物实验室公司 合成子的形成
WO2016040602A1 (en) 2014-09-11 2016-03-17 Epicentre Technologies Corporation Reduced representation bisulfite sequencing using uracil n-glycosylase (ung) and endonuclease iv
DK3201355T3 (en) 2014-09-30 2019-10-14 Illumina Inc Modified polymerases for improved incorporation of nucleotide analogues
US9897791B2 (en) 2014-10-16 2018-02-20 Illumina, Inc. Optical scanning systems for in situ genetic analysis
EP3215616B1 (de) 2014-11-05 2019-12-18 Illumina Cambridge Limited Reduzierung von dns-schäden bei probenvorbereitung und sequenzierung durch verwendung von siderophor-chelatoren
GB201419731D0 (en) 2014-11-05 2014-12-17 Illumina Cambridge Ltd Sequencing from multiple primers to increase data rate and density
CN107208019B (zh) 2014-11-11 2021-01-01 伊鲁米纳剑桥有限公司 用于核酸单克隆簇的产生和测序的方法和阵列
US10329545B2 (en) 2014-11-14 2019-06-25 Illumina, Inc. Polymerases
EP3725893A1 (de) 2015-02-10 2020-10-21 Illumina, Inc. Zusammensetzungen zur analyse von zellkomponenten
EP3271073B1 (de) 2015-03-20 2019-06-12 Illumina, Inc. Fluidikkassette zur verwendung in vertikaler oder weitgehend vertikaler position
EP3277839B1 (de) 2015-03-31 2020-12-02 Illumina Cambridge Limited Oberfläche für conkatemerization von matrizen
SG11201707515SA (en) 2015-04-10 2017-10-30 Spatial Transcriptomics Ab Spatially distinguished, multiplex nucleic acid analysis of biological specimens
WO2016182984A1 (en) 2015-05-08 2016-11-17 Centrillion Technology Holdings Corporation Disulfide-linked reversible terminators
GB201508858D0 (en) 2015-05-22 2015-07-01 Illumina Cambridge Ltd Polymethine compounds with long stokes shifts and their use as fluorescent labels
WO2016196358A1 (en) 2015-05-29 2016-12-08 Epicentre Technologies Corporation Methods of analyzing nucleic acids
EP3303614B1 (de) 2015-05-29 2020-03-11 Illumina Cambridge Limited Verbesserte verwendung von oberflächenprimern bei clustern
CN107922966B (zh) 2015-07-06 2022-06-28 伊卢米纳剑桥有限公司 用于核酸扩增的样品制备
ES2888626T3 (es) 2015-07-27 2022-01-05 Illumina Inc Cartografiado espacial de información de secuencia de ácidos nucleicos
IL295355B2 (en) 2015-08-14 2023-11-01 Illumina Inc Systems And Methods Using Magnetically-Responsive Sensors For Determining A Genetic Characteristic
CN116338219A (zh) 2015-08-24 2023-06-27 亿明达股份有限公司 用于生物和化学测定的线路内蓄压器和流量控制系统
EP4006150A1 (de) 2015-09-09 2022-06-01 QIAGEN GmbH Polymeraseenzym
US10450598B2 (en) 2015-09-11 2019-10-22 Illumina, Inc. Systems and methods for obtaining a droplet having a designated concentration of a substance-of-interest
GB201516987D0 (en) 2015-09-25 2015-11-11 Illumina Cambridge Ltd Polymethine compounds and their use as fluorescent labels
US10577643B2 (en) 2015-10-07 2020-03-03 Illumina, Inc. Off-target capture reduction in sequencing techniques
WO2017062965A1 (en) * 2015-10-08 2017-04-13 Arizona Board Of Regents On Behalf Of Arizona State University Highly sensitive optical sensor for polymerase screening
EP3370731A4 (de) 2015-11-06 2019-06-12 Qiagen Sciences, LLC Nukleotidanaloga
US11421264B2 (en) 2015-11-06 2022-08-23 IsoPlexis Corporation Thiol-containing cleave reagents and oxidative wash
US10253352B2 (en) 2015-11-17 2019-04-09 Omniome, Inc. Methods for determining sequence profiles
AU2017299803B2 (en) 2016-07-22 2023-06-29 Illumina, Inc. Single cell whole genome libraries and combinatorial indexing methods of making thereof
CA3037917C (en) 2016-09-22 2024-05-28 Illumina, Inc. Somatic copy number variation detection
US10385214B2 (en) 2016-09-30 2019-08-20 Illumina Cambridge Limited Fluorescent dyes and their uses as biomarkers
MY194951A (en) 2016-10-14 2022-12-28 Illumina Inc Cartridge assembly
WO2018114710A1 (en) 2016-12-22 2018-06-28 Illumina Cambridge Limited Coumarin compounds and their uses as fluorescent labels
HUE071446T2 (hu) 2017-01-04 2025-09-28 Mgi Tech Co Ltd Lépésenkénti szekvenálás nem jelölt reverzibilis terminátorokkal vagy természetes nukleotidokkal
EP3566158B1 (de) 2017-01-06 2022-04-20 Illumina, Inc. Phasenlagenkorrektur
US20200090784A1 (en) 2017-01-17 2020-03-19 Illumina, Inc. Oncogenic splice variant determination
AU2017394645B2 (en) 2017-01-20 2020-01-23 Pacific Biosciences Of California, Inc. Genotyping by polymerase binding
US10415029B2 (en) 2017-01-20 2019-09-17 Omniome, Inc. Allele-specific capture of nucleic acids
EP3580350B1 (de) 2017-02-13 2020-11-18 Qiagen Sciences, LLC Polymeraseenzym aus pyrococcus furiosus
US20220145272A1 (en) * 2017-02-13 2022-05-12 IsoPlexis Corporation Polymerase enzyme from pyrococcus abyssi
WO2018148723A1 (en) 2017-02-13 2018-08-16 Qiagen Waltham Inc. Polymerase enzyme from pyrococcus abyssi
WO2018148724A1 (en) 2017-02-13 2018-08-16 Qiagen Waltham Inc. Polymerase enzyme from pyrococcus furiosus
WO2018148726A1 (en) 2017-02-13 2018-08-16 Qiagen Waltham Inc. Polymerase enzyme from phage t4
EP3580334A1 (de) * 2017-02-13 2019-12-18 QIAGEN GmbH Polymeraseenzyme aus phage t4
US20200002689A1 (en) 2017-02-13 2020-01-02 Qiagen Sciences, Llc Polymerase enzyme from 9°n
WO2018148727A1 (en) 2017-02-13 2018-08-16 Qiagen Waltham Inc. Polymerase enzyme from 9°n
EP3601611B1 (de) 2017-03-24 2022-01-05 Life Technologies Corporation Polynukleotidadapter und verfahren zur verwendung davon
SG11201909914RA (en) 2017-04-23 2019-11-28 Illumina Cambridge Ltd Compositions and methods for improving sample identification in indexed nucleic acid libraries
EP3615692A1 (de) 2017-04-23 2020-03-04 Illumina Cambridge Limited Zusammensetzungen und verfahren zur verbesserung der probenidentifikation in indizierten nukleinsäurebibliotheken
CA3059952C (en) 2017-04-23 2023-04-18 Illumina Cambridge Limited Compositions and methods for improving sample identification in indexed nucleic acid libraries
RU2770879C2 (ru) 2017-06-07 2022-04-22 Орегон Хэлт Энд Сайенс Юниверсити Полногеномные библиотеки отдельных клеток для бисульфитного секвенирования
EP3642362B1 (de) 2017-06-20 2025-10-15 Illumina, Inc. Verfahren zur adressierung von ineffizienzen bei amplifikationsreaktionen
GB201711219D0 (en) 2017-07-12 2017-08-23 Illumina Cambridge Ltd Short pendant arm linkers for nucleotides in sequencing applications
WO2019027767A1 (en) 2017-07-31 2019-02-07 Illumina Inc. SEQUENCING SYSTEM COMPRISING AGGREGATION OF MULTIPLEXED BIOLOGICAL SAMPLES
CN111032883B (zh) 2017-08-01 2024-05-03 深圳华大智造科技有限公司 核酸测序方法
FI3662083T3 (fi) 2017-08-01 2024-11-20 Illumina Inc Geneettisen materiaalin ja kirjastovalmisteen spatiaalinen indeksointi hydrogeelihelmiä ja virtauskennoja käyttämällä
JP6998404B2 (ja) 2017-08-01 2022-02-04 深▲セン▼恒特基因有限公司 標的ヌクレオチド配列の富化及び決定方法
WO2019030149A1 (en) 2017-08-07 2019-02-14 Dna Script VARIANTS OF FAMILY A DNA POLYMERASE AND USES THEREOF
WO2019051250A1 (en) * 2017-09-08 2019-03-14 Sigma-Aldrich Co. Llc SYNTHESIS OF POLYNUCLEOTIDES DEPENDING ON A MATRIX AND POLYMERASE MEDIATION
EP3684955A1 (de) 2017-09-20 2020-07-29 Regeneron Pharmaceuticals, Inc. Immuntherapieverfahren für patienten, deren tumore eine hohe passagiergenmutationsbelastung tragen
GB201716931D0 (en) 2017-10-16 2017-11-29 Illumina Cambridge Ltd New fluorescent compounds and their use as biomarkers
KR102500210B1 (ko) 2017-11-06 2023-02-15 일루미나, 인코포레이티드 핵산 색인 기술
JP6955035B2 (ja) 2017-11-16 2021-10-27 イルミナ インコーポレイテッド マイクロサテライト不安定性を決定するためのシステム及び方法
US12040047B2 (en) 2017-11-30 2024-07-16 Illumina, Inc. Validation methods and systems for sequence variant calls
US10752887B2 (en) 2018-01-08 2020-08-25 Dna Script Variants of terminal deoxynucleotidyl transferase and uses thereof
CA3065934A1 (en) 2018-01-08 2019-07-11 Illumina, Inc. High-throughput sequencing with semiconductor-based detection
US11378544B2 (en) 2018-01-08 2022-07-05 Illumina, Inc. High-throughput sequencing with semiconductor-based detection
US10844430B2 (en) 2018-01-24 2020-11-24 Qiagen Sciences, Llc DNA sequencing reaction additive
KR102299751B1 (ko) 2018-03-09 2021-09-08 일루미나 케임브리지 리미티드 일반화된 스토캐스틱 초-해상 시퀀싱
JP6974504B2 (ja) 2018-04-02 2021-12-01 イルミナ インコーポレイテッド 配列に基づく遺伝子試験のための対照を作製するための組成物及び方法
IL271454B2 (en) 2018-05-17 2025-04-01 Illumina Inc Rapid single-cell genetic sequencing with low Aggregation bias
US20210102194A1 (en) 2018-06-04 2021-04-08 Illumina, Inc. High-throughput single-cell transcriptome libraries and methods of making and of using
NL2021377B1 (en) 2018-07-03 2020-01-08 Illumina Inc Interposer with first and second adhesive layers
WO2020047005A1 (en) 2018-08-28 2020-03-05 10X Genomics, Inc. Resolving spatial arrays
CN113366117B (zh) 2018-08-28 2025-08-05 10X基因组学股份有限公司 用于生物样品中转座酶介导的空间标记和分析基因组dna的方法
US11519033B2 (en) 2018-08-28 2022-12-06 10X Genomics, Inc. Method for transposase-mediated spatial tagging and analyzing genomic DNA in a biological sample
WO2020056044A1 (en) 2018-09-11 2020-03-19 Singular Genomics Systems, Inc. Modified archaeal family b polymerases
US11242512B2 (en) 2018-09-17 2022-02-08 Omniome, Inc. Engineered polymerases for improved sequencing
US12258368B2 (en) 2018-09-28 2025-03-25 Centrillion Technology Holdings Corporation Disulfide-linked reversible terminators
IL299237B2 (en) 2018-10-31 2024-12-01 Illumina Inc Polymerases, compositions, and methods of use
NL2022043B1 (en) 2018-11-21 2020-06-03 Akershus Univ Hf Tagmentation-Associated Multiplex PCR Enrichment Sequencing
US11001816B2 (en) 2018-12-05 2021-05-11 Illumina, Inc. Polymerases, compositions, and methods of use
WO2020123316A2 (en) 2018-12-10 2020-06-18 10X Genomics, Inc. Methods for determining a location of a biological analyte in a biological sample
US11293061B2 (en) 2018-12-26 2022-04-05 Illumina Cambridge Limited Sequencing methods using nucleotides with 3′ AOM blocking group
US11649485B2 (en) 2019-01-06 2023-05-16 10X Genomics, Inc. Generating capture probes for spatial analysis
US11926867B2 (en) 2019-01-06 2024-03-12 10X Genomics, Inc. Generating capture probes for spatial analysis
CN114174531A (zh) 2019-02-28 2022-03-11 10X基因组学有限公司 用空间条码化寡核苷酸阵列对生物分析物进行概况分析
MX2021003746A (es) 2019-03-01 2021-06-23 Illumina Inc Genotecas de núcleos únicos y células únicas de alto rendimiento y métodos para elaborarlas y usarlas.
JP7587424B2 (ja) 2019-03-01 2024-11-20 イルミナ ケンブリッジ リミテッド 環外アミン置換クマリン化合物およびそれらの蛍光標識としての使用
US12110546B2 (en) 2019-03-01 2024-10-08 Illumina Cambridge Limited Tertiary amine substituted coumarin compounds and uses as fluorescent labels
NL2023327B1 (en) 2019-03-01 2020-09-17 Illumina Inc Multiplexed fluorescent detection of analytes
WO2020178231A1 (en) 2019-03-01 2020-09-10 Illumina, Inc. Multiplexed fluorescent detection of analytes
CN114127309A (zh) 2019-03-15 2022-03-01 10X基因组学有限公司 使用空间阵列进行单细胞测序的方法
NL2023311B9 (en) 2019-03-21 2021-03-12 Illumina Inc Artificial intelligence-based generation of sequencing metadata
US11210554B2 (en) 2019-03-21 2021-12-28 Illumina, Inc. Artificial intelligence-based generation of sequencing metadata
US11676685B2 (en) 2019-03-21 2023-06-13 Illumina, Inc. Artificial intelligence-based quality scoring
NL2023316B1 (en) 2019-03-21 2020-09-28 Illumina Inc Artificial intelligence-based sequencing
WO2020191387A1 (en) 2019-03-21 2020-09-24 Illumina, Inc. Artificial intelligence-based base calling
NL2023314B1 (en) 2019-03-21 2020-09-28 Illumina Inc Artificial intelligence-based quality scoring
NL2023312B1 (en) 2019-03-21 2020-09-28 Illumina Inc Artificial intelligence-based base calling
NL2023310B1 (en) 2019-03-21 2020-09-28 Illumina Inc Training data generation for artificial intelligence-based sequencing
EP3887542A1 (de) 2019-03-22 2021-10-06 10X Genomics, Inc. Dreidimensionale räumliche analyse
US11421271B2 (en) 2019-03-28 2022-08-23 Illumina Cambridge Limited Methods and compositions for nucleic acid sequencing using photoswitchable labels
US11423306B2 (en) 2019-05-16 2022-08-23 Illumina, Inc. Systems and devices for characterization and performance analysis of pixel-based sequencing
US11593649B2 (en) 2019-05-16 2023-02-28 Illumina, Inc. Base calling using convolutions
WO2020243579A1 (en) 2019-05-30 2020-12-03 10X Genomics, Inc. Methods of detecting spatial heterogeneity of a biological sample
US11512295B2 (en) 2019-09-12 2022-11-29 Singular Genomics Systems, Inc. Modified thermoccocus polymerases
JP2022549196A (ja) 2019-09-23 2022-11-24 ディーエヌエー スクリプト ポリヌクレオチドの鋳型なし酵素的合成における長配列収率の増加
EP4468252A3 (de) 2019-10-01 2025-02-19 10x Genomics, Inc. Systeme und methoden zur identifizierung morphologischer muster in gewebeproben
WO2021091611A1 (en) 2019-11-08 2021-05-14 10X Genomics, Inc. Spatially-tagged analyte capture agents for analyte multiplexing
WO2021092433A2 (en) 2019-11-08 2021-05-14 10X Genomics, Inc. Enhancing specificity of analyte binding
US20210139867A1 (en) 2019-11-08 2021-05-13 Omniome, Inc. Engineered polymerases for improved sequencing by binding
WO2021102003A1 (en) 2019-11-18 2021-05-27 10X Genomics, Inc. Systems and methods for tissue classification
CN115210760B (zh) 2019-11-21 2023-08-01 10X基因组学有限公司 分析物的空间分析
EP4065646A1 (de) 2019-11-27 2022-10-05 Illumina Cambridge Limited Cyclooctatetraen enthaltende farbstoffe und zusammensetzungen
AU2020407267A1 (en) 2019-12-18 2022-06-16 F. Hoffmann-La Roche Ag Methods of sequencing by synthesis using a consecutive labeling scheme
KR20220118295A (ko) 2019-12-19 2022-08-25 일루미나, 인코포레이티드 고 처리량 단일 세포 라이브러리, 및 이의 제조 방법 및 사용 방법
CN115038794A (zh) 2019-12-23 2022-09-09 10X基因组学有限公司 在基于分区的测定中使用固定生物样品的组合物和方法
CN121022991A (zh) 2019-12-23 2025-11-28 10X基因组学有限公司 使用rna模板化连接进行空间分析的方法
CN110938611B (zh) * 2019-12-31 2021-05-04 莫纳(武汉)生物科技有限公司 一种dna聚合酶及其制备方法和应用
US12365942B2 (en) 2020-01-13 2025-07-22 10X Genomics, Inc. Methods of decreasing background on a spatial array
US12405264B2 (en) 2020-01-17 2025-09-02 10X Genomics, Inc. Electrophoretic system and method for analyte capture
US11732299B2 (en) 2020-01-21 2023-08-22 10X Genomics, Inc. Spatial assays with perturbed cells
US11702693B2 (en) 2020-01-21 2023-07-18 10X Genomics, Inc. Methods for printing cells and generating arrays of barcoded cells
US20210230681A1 (en) 2020-01-24 2021-07-29 10X Genomics, Inc. Methods for spatial analysis using proximity ligation
US12076701B2 (en) 2020-01-31 2024-09-03 10X Genomics, Inc. Capturing oligonucleotides in spatial transcriptomics
US11898205B2 (en) 2020-02-03 2024-02-13 10X Genomics, Inc. Increasing capture efficiency of spatial assays
US12110541B2 (en) 2020-02-03 2024-10-08 10X Genomics, Inc. Methods for preparing high-resolution spatial arrays
US12112833B2 (en) 2020-02-04 2024-10-08 10X Genomics, Inc. Systems and methods for index hopping filtering
US11732300B2 (en) 2020-02-05 2023-08-22 10X Genomics, Inc. Increasing efficiency of spatial analysis in a biological sample
WO2021158925A1 (en) 2020-02-07 2021-08-12 10X Genomics, Inc. Quantitative and automated permeabilization performance evaluation for spatial transcriptomics
US12421558B2 (en) 2020-02-13 2025-09-23 10X Genomics, Inc. Systems and methods for joint interactive visualization of gene expression and DNA chromatin accessibility
US12281357B1 (en) 2020-02-14 2025-04-22 10X Genomics, Inc. In situ spatial barcoding
CN115485391A (zh) 2020-02-17 2022-12-16 10X基因组学有限公司 染色质相互作用的原位分析
IL295560A (en) 2020-02-20 2022-10-01 Illumina Inc Artificial intelligence-based many-to-many base calling
US20210265016A1 (en) 2020-02-20 2021-08-26 Illumina, Inc. Data Compression for Artificial Intelligence-Based Base Calling
US12354008B2 (en) 2020-02-20 2025-07-08 Illumina, Inc. Knowledge distillation and gradient pruning-based compression of artificial intelligence-based base caller
US20210265015A1 (en) 2020-02-20 2021-08-26 Illumina, Inc. Hardware Execution and Acceleration of Artificial Intelligence-Based Base Caller
EP4107285B1 (de) 2020-02-21 2024-10-09 10X Genomics, Inc. Verfahren und zusammensetzungen für integrierten räumlichen in-situ-test
US11891654B2 (en) 2020-02-24 2024-02-06 10X Genomics, Inc. Methods of making gene expression libraries
US11034942B1 (en) 2020-02-27 2021-06-15 Singular Genomics Systems, Inc. Modified pyrococcus polymerases and uses thereof
US12188085B2 (en) 2020-03-05 2025-01-07 10X Genomics, Inc. Three-dimensional spatial transcriptomics with sequencing readout
CN115427586B (zh) 2020-03-18 2025-03-28 深圳华大智造科技股份有限公司 大规模平行测序中的恢复阶段
ES2965354T3 (es) 2020-04-22 2024-04-12 10X Genomics Inc Métodos para análisis espacial que usan eliminación de ARN elegido como diana
US20230203592A1 (en) 2020-05-05 2023-06-29 Akershus Universitetssykehus Hf Compositions and methods for characterizing bowel cancer
US11188778B1 (en) 2020-05-05 2021-11-30 Illumina, Inc. Equalization-based image processing and spatial crosstalk attenuator
JP2023525470A (ja) 2020-05-08 2023-06-16 イルミナ インコーポレイテッド ゲノムシーケンシング及び検出手法
CA3177286A1 (en) 2020-05-12 2021-11-18 Illumina Inc. Generating nucleic acids with modified bases using recombinant terminal deoxynucleotidyl transferase
WO2021237087A1 (en) 2020-05-22 2021-11-25 10X Genomics, Inc. Spatial analysis to detect sequence variants
ES2989052T3 (es) 2020-05-22 2024-11-25 10X Genomics Inc Medición espacio-temporal simultánea de la expresión génica y la actividad celular
WO2021242834A1 (en) 2020-05-26 2021-12-02 10X Genomics, Inc. Method for resetting an array
US12031177B1 (en) 2020-06-04 2024-07-09 10X Genomics, Inc. Methods of enhancing spatial resolution of transcripts
ES2981265T3 (es) 2020-06-08 2024-10-08 10X Genomics Inc Métodos para determinar un margen quirúrgico y métodos de uso del mismo
WO2021252617A1 (en) 2020-06-09 2021-12-16 Illumina, Inc. Methods for increasing yield of sequencing libraries
WO2021252591A1 (en) 2020-06-10 2021-12-16 10X Genomics, Inc. Methods for determining a location of an analyte in a biological sample
JP2023531009A (ja) 2020-06-22 2023-07-20 イルミナ ケンブリッジ リミテッド ３’アセタールブロッキング基を有するヌクレオシド及びヌクレオチド
EP4172362B1 (de) 2020-06-25 2024-09-18 10X Genomics, Inc. Räumliche analyse der dna-methylierung
WO2022006081A1 (en) 2020-06-30 2022-01-06 Illumina, Inc. Catalytically controlled sequencing by synthesis to produce scarless dna
US12168801B1 (en) 2020-07-02 2024-12-17 10X Genomics, Inc. Hybrid/capture probe designs for full-length cDNA
US11981960B1 (en) 2020-07-06 2024-05-14 10X Genomics, Inc. Spatial analysis utilizing degradable hydrogels
US11761038B1 (en) 2020-07-06 2023-09-19 10X Genomics, Inc. Methods for identifying a location of an RNA in a biological sample
US12209280B1 (en) 2020-07-06 2025-01-28 10X Genomics, Inc. Methods of identifying abundance and location of an analyte in a biological sample using second strand synthesis
CN116018412A (zh) 2020-07-08 2023-04-25 Illumina公司 作为转座体载体的小珠
US11981964B2 (en) 2020-07-28 2024-05-14 Illumina Cambridge Limited Substituted coumarin dyes and uses as fluorescent labels
US11981958B1 (en) 2020-08-20 2024-05-14 10X Genomics, Inc. Methods for spatial analysis using DNA capture
US20220067489A1 (en) 2020-08-28 2022-03-03 Illumina, Inc. Detecting and Filtering Clusters Based on Artificial Intelligence-Predicted Base Calls
AU2021338589B2 (en) * 2020-09-11 2025-09-04 Illumina Singapore Pte. Ltd. Detecting nucleic acids using sugar-lectin couplings
US11926822B1 (en) 2020-09-23 2024-03-12 10X Genomics, Inc. Three-dimensional spatial analysis
US12071667B2 (en) 2020-11-04 2024-08-27 10X Genomics, Inc. Sequence analysis using meta-stable nucleic acid molecules
US11827935B1 (en) 2020-11-19 2023-11-28 10X Genomics, Inc. Methods for spatial analysis using rolling circle amplification and detection probes
US20220170010A1 (en) 2020-12-02 2022-06-02 Illumina Software, Inc. System and method for detection of genetic alterations
EP4012046A1 (de) 2020-12-11 2022-06-15 10X Genomics, Inc. Verfahren und zusammensetzungen zur multimodalen in-situ-analyse
US20220195517A1 (en) 2020-12-17 2022-06-23 Illumina Cambridge Limited Long stokes shift chromenoquinoline dyes and uses in sequencing applications
US20220195196A1 (en) 2020-12-17 2022-06-23 Illumina Cambridge Limited Alkylpyridinium coumarin dyes and uses in sequencing applications
US20220195516A1 (en) 2020-12-17 2022-06-23 Illumina Cambridge Limited Methods, systems and compositions for nucleic acid sequencing
EP4121555A1 (de) 2020-12-21 2023-01-25 10X Genomics, Inc. Verfahren, zusammensetzungen und systeme zur erfassung von sonden und/oder barcodes
US20220195518A1 (en) 2020-12-22 2022-06-23 Illumina Cambridge Limited Methods and compositions for nucleic acid sequencing
US12060603B2 (en) 2021-01-19 2024-08-13 10X Genomics, Inc. Methods for internally controlled in situ assays using padlock probes
US20220235403A1 (en) 2021-01-26 2022-07-28 10X Genomics, Inc. Nucleic acid analog probes for in situ analysis
US20220282319A1 (en) 2021-03-03 2022-09-08 10X Genomics, Inc. Analyte detection in situ using nucleic acid origami
WO2022197752A1 (en) 2021-03-16 2022-09-22 Illumina, Inc. Tile location and/or cycle based weight set selection for base calling
EP4308718A4 (de) * 2021-03-19 2025-09-10 Mgi Tech Co Ltd Polymerasen zur effizienten einbindung von nukleotiden mit 3'-phosphat und anderen 3'-terminatoren
AU2022248999A1 (en) 2021-03-31 2023-02-02 Illumina, Inc. Artificial intelligence-based base caller with contextual awareness
IL307159A (en) 2021-03-31 2023-11-01 Illumina Cambridge Ltd Nucleic acid library sequencing techniques with adapter dimer detection
JP7719206B2 (ja) 2021-04-02 2025-08-05 イルミナ インコーポレイテッド 配列決定のためのヌクレオチド－試料スライド内の泡を検出するための機械学習モデル
EP4428246B1 (de) 2021-04-14 2025-12-24 10X Genomics, Inc. Verfahren zur messung der fehllokalisierung eines analyten
CN117242192A (zh) 2021-04-14 2023-12-15 伊鲁米纳新加坡有限公司 多核苷酸瓶刷状聚合物的合成
US20220336057A1 (en) 2021-04-15 2022-10-20 Illumina, Inc. Efficient voxelization for deep learning
US12217829B2 (en) 2021-04-15 2025-02-04 Illumina, Inc. Artificial intelligence-based analysis of protein three-dimensional (3D) structures
FR3121936A1 (fr) 2021-04-20 2022-10-21 Naos Institute Of Life Science Methode de criblage d’ingredients et de compositions topiques ecobiologiques, et son utilisation pour realiser un soin cosmetique de restauration du microbiote cutane
WO2022232425A2 (en) 2021-04-29 2022-11-03 Illumina, Inc. Amplification techniques for nucleic acid characterization
EP4334327A1 (de) 2021-05-05 2024-03-13 Illumina Cambridge Limited Fluoreszierende farbstoffe mit kondensierten bis-bor-heterocyclen und verwendungen in der sequenzierung
WO2022236054A1 (en) 2021-05-06 2022-11-10 10X Genomics, Inc. Methods for increasing resolution of spatial analysis
US20220396832A1 (en) 2021-05-20 2022-12-15 Illumina Cambridge Limited Compositions and methods for sequencing by synthesis
CN117396613A (zh) 2021-06-01 2024-01-12 10X基因组学有限公司 用于分析物检测和探针解析的方法和组合物
WO2022256422A1 (en) 2021-06-02 2022-12-08 10X Genomics, Inc. Sample analysis using asymmetric circularizable probes
US20220403450A1 (en) 2021-06-03 2022-12-22 Illumina Software, Inc. Systems and methods for sequencing nucleotides using two optical channels
EP4582555A3 (de) 2021-06-03 2025-10-22 10X Genomics, Inc. Verfahren, zusammensetzungen, kits und systeme zur verbesserung der analyterfassung zur räumlichen analyse
IL309440A (en) * 2021-06-18 2024-02-01 Element Biosciences Inc Engineered polymerases
US12139727B2 (en) 2021-06-18 2024-11-12 Element Biosciences, Inc. Engineered polymerases
EP4359555A1 (de) 2021-06-23 2024-05-01 Illumina, Inc. Zusammensetzungen, verfahren, kits, kartuschen und systeme zur sequenzierung von reagenzien
BR112023026615A2 (pt) 2021-06-29 2024-03-05 Illumina Inc Métrica de relação sinal-ruído para determinar chamadas de bases de nucleotídeos e qualidade de chamadas de bases
WO2023278966A1 (en) 2021-06-29 2023-01-05 Illumina, Inc. Machine-learning model for generating confidence classifications for genomic coordinates
WO2023278184A1 (en) 2021-06-29 2023-01-05 Illumina, Inc. Methods and systems to correct crosstalk in illumination emitted from reaction sites
WO2023278609A1 (en) 2021-06-29 2023-01-05 Illumina, Inc. Self-learned base caller, trained using organism sequences
US20230005253A1 (en) 2021-07-01 2023-01-05 Illumina, Inc. Efficient artificial intelligence-based base calling of index sequences
WO2023287617A1 (en) 2021-07-13 2023-01-19 Illumina, Inc. Methods and systems for real time extraction of crosstalk in illumination emitted from reaction sites
WO2023288225A1 (en) 2021-07-13 2023-01-19 10X Genomics, Inc. Methods for preparing polymerized matrix with controllable thickness
US11455487B1 (en) 2021-10-26 2022-09-27 Illumina Software, Inc. Intensity extraction and crosstalk attenuation using interpolation and adaptation for base calling
WO2023003757A1 (en) 2021-07-19 2023-01-26 Illumina Software, Inc. Intensity extraction with interpolation and adaptation for base calling
US20230021577A1 (en) 2021-07-23 2023-01-26 Illumina Software, Inc. Machine-learning model for recalibrating nucleotide-base calls
EP4377960A1 (de) 2021-07-28 2024-06-05 Illumina, Inc. Kalibrierung der qualitätsbewertung von basisrufsystemen
US12139751B2 (en) 2021-07-30 2024-11-12 10X Genomics, Inc. Circularizable probes for in situ analysis
CA3224093A1 (en) 2021-07-30 2023-02-02 Jorge Ivan Hernandez Neuta Methods and compositions for synchronizing reactions in situ
US12391984B2 (en) 2021-08-03 2025-08-19 10X Genomics, Inc. Compositions and methods for rolling circle amplification
US12460251B2 (en) 2021-08-03 2025-11-04 10X Genomics, Inc. Stabilization and/or compaction of nucleic acid molecules
CN118043476A (zh) 2021-08-03 2024-05-14 10X基因组学有限公司 核酸多联体及其稳定和/或压缩方法
KR20240035413A (ko) 2021-08-03 2024-03-15 일루미나, 인코포레이티드 다중 염기 호출자 모델을 사용한 염기 호출
US12077789B2 (en) 2021-08-14 2024-09-03 Illumina, Inc. Polymerases, compositions, and methods of use
EP4326898B1 (de) 2021-08-16 2024-07-31 10X Genomics, Inc. Sonden mit einem geteilten strichcodebereich und verfahren zur verwendung
CN117580961A (zh) 2021-09-01 2024-02-20 Illumina公司 用于加速碱基判读的幅度调制
ES3011462T3 (en) 2021-09-01 2025-04-07 10X Genomics Inc Methods for blocking a capture probe on a spatial array
CN117561573A (zh) 2021-09-17 2024-02-13 因美纳有限公司 从碱基判读错误模式自动鉴定核苷酸测序中的故障来源
JP2024535664A (ja) 2021-09-21 2024-10-02 イルミナ インコーポレイテッド 帰属ハプロタイプを用いたグラフ参照ゲノム及び塩基コールアプローチ
WO2023049215A1 (en) 2021-09-22 2023-03-30 Illumina, Inc. Compressed state-based base calling
EP4419707A1 (de) 2021-11-10 2024-08-28 10X Genomics, Inc. Verfahren, zusammensetzungen und kits zur bestimmung der position eines analyten in einer biologischen probe
EP4305195A2 (de) 2021-12-01 2024-01-17 10X Genomics, Inc. Verfahren, zusammensetzungen und systeme für verbesserten in-situ-nachweis von analyten und raumanalyse
KR20240116364A (ko) 2021-12-02 2024-07-29 일루미나, 인코포레이티드 뉴클레오티드 염기 호출을 결정하기 위한 클러스터별 신호 교정 생성
WO2023107622A1 (en) 2021-12-10 2023-06-15 Illumina, Inc. Parallel sample and index sequencing
WO2023114896A1 (en) 2021-12-16 2023-06-22 Illumina Cambridge Limited Methods for metal directed cleavage of surface-bound polynucleotides
CN117813399A (zh) 2021-12-20 2024-04-02 因美纳有限公司 用于化学裂解表面结合的多核苷酸的高碘酸盐组合物和方法
CN117940577A (zh) 2021-12-20 2024-04-26 因美纳有限公司 用于化学裂解表面结合的多核苷酸的高碘酸盐组合物和方法
WO2023122363A1 (en) 2021-12-23 2023-06-29 Illumina Software, Inc. Dynamic graphical status summaries for nucelotide sequencing
US20230215515A1 (en) 2021-12-23 2023-07-06 Illumina Software, Inc. Facilitating secure execution of external workflows for genomic sequencing diagnostics
WO2023129898A2 (en) 2021-12-27 2023-07-06 10X Genomics, Inc. Methods and compositions for rolling circle amplification
US20230207050A1 (en) 2021-12-28 2023-06-29 Illumina Software, Inc. Machine learning model for recalibrating nucleotide base calls corresponding to target variants
CA3242624A1 (en) 2021-12-29 2023-07-06 Shannon Whitmore Automatically switching variant analysis model versions for genomic analysis applications
EP4466376A1 (de) 2022-01-21 2024-11-27 10X Genomics, Inc. Mehrere auslesesignale zur analyse einer probe
US11788075B2 (en) 2022-02-18 2023-10-17 Element Biosciences, Inc. Engineered polymerases with reduced sequence-specific errors
US20240254537A1 (en) 2022-02-23 2024-08-01 Insitro, Inc. Pooled optical screening and transcriptional measurements of cells comprising barcoded genetic perturbations
AU2023225949A1 (en) 2022-02-25 2024-01-18 Illumina, Inc. Machine-learning models for detecting and adjusting values for nucleotide methylation levels
WO2023164660A1 (en) 2022-02-25 2023-08-31 Illumina, Inc. Calibration sequences for nucelotide sequencing
WO2023175021A1 (en) 2022-03-15 2023-09-21 Illumina, Inc. Methods of preparing loop fork libraries
WO2023183937A1 (en) 2022-03-25 2023-09-28 Illumina, Inc. Sequence-to-sequence base calling
US20230304086A1 (en) 2022-03-28 2023-09-28 Illumina Cambridge Limited Labeled avidin and methods for sequencing
US20230313292A1 (en) 2022-03-29 2023-10-05 Illumina Cambridge Limited Chromenoquinoline dyes and uses in sequencing
CA3223037A1 (en) 2022-03-30 2023-10-05 Oliver MILLER Methods for chemical cleavage of surface-bound polynucleotides
EP4499659A1 (de) 2022-03-31 2025-02-05 Illumina, Inc. Nukleoside und nukleotide mit 3' vinyl blockierender gruppe, die sich für die sequenzierung durch synthese eignen
EP4499870A1 (de) 2022-03-31 2025-02-05 Illumina, Inc. Zusammensetzungen und verfahren zur verbesserung von sequenzierungssignalen
AU2023243205A1 (en) 2022-04-01 2024-09-19 10X Genomics, Inc. Compositions and methods for targeted masking of autofluorescence
CN119234045A (zh) 2022-04-06 2024-12-31 10X基因组学有限公司 用于多重细胞分析的方法
WO2023196572A1 (en) 2022-04-07 2023-10-12 Illumina Singapore Pte. Ltd. Altered cytidine deaminases and methods of use
WO2023196528A1 (en) 2022-04-08 2023-10-12 Illumina, Inc. Aptamer dynamic range compression and detection techniques
EP4515547A1 (de) 2022-04-26 2025-03-05 Illumina, Inc. Maschinenlernmodelle zur auswahl von oligonukleotidsonden für arraytechnologien
WO2023209606A1 (en) 2022-04-29 2023-11-02 Illumina Cambridge Limited Methods and systems for encapsulating lyophilised microspheres
EP4519674A1 (de) 2022-05-06 2025-03-12 10X Genomics, Inc. Analyse von antigen- und antigenrezeptorinteraktionen
EP4523215A1 (de) 2022-05-10 2025-03-19 Illumina Inc. Adaptives neuronales netzwerk zur nucelotidsequenzierung
WO2023225095A1 (en) 2022-05-18 2023-11-23 Illumina Cambridge Limited Preparation of size-controlled nucleic acid fragments
WO2023232829A1 (en) 2022-05-31 2023-12-07 Illumina, Inc Compositions and methods for nucleic acid sequencing
US20240035071A1 (en) 2022-06-17 2024-02-01 10X Genomics, Inc. Catalytic de-crosslinking of samples for in situ analysis
US20230420080A1 (en) 2022-06-24 2023-12-28 Illumina Software, Inc. Split-read alignment by intelligently identifying and scoring candidate split groups
EP4544552A1 (de) 2022-06-27 2025-04-30 Illumina Inc. Beschleuniger für ein genotyp-einschreibungsmodell
US20230420082A1 (en) 2022-06-27 2023-12-28 Illumina Software, Inc. Generating and implementing a structural variation graph genome
EP4544554A1 (de) 2022-06-27 2025-04-30 Illumina, Inc. Verbesserte genotypisierung des menschlichen leukozytenantigens (hla)
EP4547675A1 (de) 2022-06-28 2025-05-07 Illumina, Inc. Fluoreszenzfarbstoffe mit kondensiertem tetracyclischem bis-bor-heterocyclus und verwendungen bei der sequenzierung
EP4562638A1 (de) 2022-07-26 2025-06-04 Illumina, Inc. Schnelle einzelzellen-multiomics-verarbeitung unter verwendung einer ausführbaren datei
US20240101978A1 (en) 2022-08-12 2024-03-28 10X Genomics, Inc. Puma1 polymerases and uses thereof
CN119677873A (zh) 2022-08-16 2025-03-21 10X基因组学有限公司 Ap50聚合酶及其用途
US20250361558A1 (en) 2022-08-19 2025-11-27 Illumina, Inc. Third dna base pair site-specific dna detection
EP4588051A1 (de) 2022-09-16 2025-07-23 Illumina, Inc. Clustersegmentierung und bedingter basisanruf
CN118974830A (zh) 2022-09-29 2024-11-15 因美纳有限公司 用于推算靶变体的靶变体参考组
CN119213083A (zh) 2022-09-30 2024-12-27 伊路米纳有限公司 用于减少测序期间的光损伤的组合物和方法
WO2024073043A1 (en) 2022-09-30 2024-04-04 Illumina, Inc. Methods of using cpg binding proteins in mapping modified cytosine nucleotides
CN120153070A (zh) 2022-09-30 2025-06-13 因美纳有限公司 解旋酶-胞苷脱氨酶复合物及其使用方法
US20240141427A1 (en) 2022-09-30 2024-05-02 Illumina, Inc. Polymerases, compositions, and methods of use
EP4595059A1 (de) 2022-09-30 2025-08-06 Illumina, Inc. Maschinenlernmodell zur verfeinerung von strukturvariantenanrufen
WO2024073047A1 (en) 2022-09-30 2024-04-04 Illumina, Inc. Cytidine deaminases and methods of use in mapping modified cytosine nucleotides
US20240127905A1 (en) 2022-10-05 2024-04-18 Illumina, Inc. Integrating variant calls from multiple sequencing pipelines utilizing a machine learning architecture
EP4602608A1 (de) 2022-10-11 2025-08-20 Illumina, Inc. Nachweis und korrektur von methylierungswerten aus methylierungssequenzierungsassays
EP4602344A1 (de) 2022-10-14 2025-08-20 10X Genomics, Inc. Verfahren zur analyse biologischer proben
US20240167081A1 (en) 2022-11-08 2024-05-23 10X Genomics,Inc. Immobilization methods and compositions for in situ detection
WO2024107887A1 (en) 2022-11-16 2024-05-23 10X Genomics, Inc. Methods and compositions for assessing performance of in situ assays
US12372771B2 (en) 2022-11-18 2025-07-29 10X Genomics, Inc. Systems and methods for actively mitigating vibrations
EP4627583A1 (de) 2022-11-30 2025-10-08 Illumina, Inc. Genaue vorhersage von varianten aus methylierungssequenzierungsdaten
WO2024123866A1 (en) 2022-12-09 2024-06-13 Illumina, Inc. Nucleosides and nucleotides with 3´ blocking groups and cleavable linkers
US20240218437A1 (en) 2022-12-16 2024-07-04 10X Genomics, Inc. Methods and compositions for assessing performance
US20240218424A1 (en) 2022-12-21 2024-07-04 10X Genomics, Inc. Methods for tethering ribonucleic acids in biological samples
WO2024137886A1 (en) 2022-12-21 2024-06-27 Illumina, Inc. Context-dependent base calling
US20240229131A1 (en) 2022-12-22 2024-07-11 Illumina, Inc. Transition-metal catalyst compositions and methods for sequencing by synthesis
AU2023409219A1 (en) 2022-12-22 2024-10-03 Illumina, Inc. Palladium catalyst compositions and methods for sequencing by synthesis
US20240271206A1 (en) 2022-12-27 2024-08-15 Illumina, Inc. Methods of sequencing using 3' allyl blocked nucleotides
US20240263219A1 (en) 2023-01-06 2024-08-08 10X Genomics, Inc. Methods and compositions for in situ analysis of variant sequences
AU2024219208A1 (en) 2023-02-06 2025-01-16 Illumina, Inc. Determining and removing inter-cluster light interference
AU2024235961A1 (en) 2023-03-10 2025-01-09 Illumina, Inc. Aptamer detection techniques
CN119452044A (zh) 2023-03-29 2025-02-14 因美纳公司 萘酰亚胺染料以及其在核酸测序中的用途
AU2024247902A1 (en) 2023-03-30 2024-12-12 Illumina, Inc. Compositions and methods for nucleic acid sequencing
WO2024206848A1 (en) 2023-03-30 2024-10-03 Illumina, Inc. Tandem repeat genotyping
CN119744419A (zh) 2023-05-03 2025-04-01 因美纳有限公司 用于重新校准来自现有测序数据文件的基因型检出的机器学习模型
US20240368678A1 (en) 2023-05-03 2024-11-07 10X Genomics, Inc. Methods and compositions for spatial assay
WO2024249200A1 (en) 2023-05-26 2024-12-05 Illumina, Inc. Methods for preserving methylation status during clustering
US20240404624A1 (en) 2023-05-31 2024-12-05 Illumina, Inc. Structural variant alignment and variant calling by utilizing a structural-variant reference genome
WO2024249973A2 (en) 2023-06-02 2024-12-05 Illumina, Inc. Linking human genes to clinical phenotypes using graph neural networks
WO2025006565A1 (en) 2023-06-27 2025-01-02 Illumina, Inc. Variant calling with methylation-level estimation
WO2025006874A1 (en) 2023-06-30 2025-01-02 Illumina, Inc. Machine-learning model for recalibrating genotype calls corresponding to germline variants and somatic mosaic variants
WO2025006464A1 (en) 2023-06-30 2025-01-02 Illumina, Inc. Systems and methods of sequencing polynucleotides with alternative scatterplots
WO2025006466A1 (en) 2023-06-30 2025-01-02 Illumina, Inc. Systems and methods of sequencing polynucleotides with four labeled nucleotides
WO2025006460A1 (en) 2023-06-30 2025-01-02 Illumina, Inc. Systems and methods of sequencing polynucleotides with modified bases
WO2025029831A1 (en) 2023-07-31 2025-02-06 10X Genomics, Inc. Methods and systems for targeted rna cleavage and target rna-primed rolling circle amplification
US20250075269A1 (en) 2023-08-31 2025-03-06 Illumina, Inc. Compositions and methods for nucleic acid sequencing
WO2025049720A2 (en) 2023-08-31 2025-03-06 Illumina, Inc. Aptamer dynamic range compression and detection techniques
WO2025049331A2 (en) 2023-08-31 2025-03-06 Illumina, Inc. Aptamer detection techniques
WO2025054389A1 (en) 2023-09-07 2025-03-13 Illumina, Inc. Identification of methylated cytosine using landmarks
WO2025050463A1 (en) 2023-09-08 2025-03-13 The Regents Of The University Of Michigan MICRORNA-DERIVED RNAs AND POLYPEPTIDES AND USES THEREOF
WO2025061922A1 (en) 2023-09-20 2025-03-27 Illumina, Inc. Methods for sequencing
WO2025061942A1 (en) 2023-09-20 2025-03-27 Illumina, Inc. Sequencing error identification and correction
WO2025072783A1 (en) 2023-09-28 2025-04-03 Illumina, Inc. Altered cytidine deaminases and methods of use
US20250111899A1 (en) 2023-09-29 2025-04-03 Illumina, Inc. Predicting insert lengths using primary analysis metrics
US20250111898A1 (en) 2023-09-29 2025-04-03 Illumina, Inc. Tracking and modifying cluster location on nucleotide-sample slides in real time
WO2025073113A1 (zh) * 2023-10-07 2025-04-10 深圳华大智造科技股份有限公司 Dna聚合酶突变体及其在测序中的应用
WO2025081064A2 (en) 2023-10-11 2025-04-17 Illumina, Inc. Thermophilic deaminase and methods for identifying modified cytosine
US20250137048A1 (en) 2023-10-26 2025-05-01 Illumina, Inc. 4,5-substituted naphthalimide dyes and uses in nucleic acid sequencing
WO2025090883A1 (en) 2023-10-27 2025-05-01 Illumina, Inc. Detecting variants in nucleotide sequences based on haplotype diversity
US20250163505A1 (en) 2023-11-17 2025-05-22 Illumina, Inc. Conjugated polymers or polymer dots as fluorescent labels
US20250163502A1 (en) 2023-11-22 2025-05-22 10X Genomics, Inc. Methods for processing ribonucleic acids in biological samples
WO2025123005A2 (en) 2023-12-07 2025-06-12 Illumina, Inc. Preparation and compositions of cot-1 nucleic acid
WO2025129133A1 (en) 2023-12-15 2025-06-19 Illumina, Inc. Minimal residual disease (mrd) models for determining likelihoods or probabilities of a subject comprising cancer
US20250201346A1 (en) 2023-12-18 2025-06-19 Illumina, Inc. Using machine learning models for detecting minimum residual disease (mrd) in a subject
US20250197933A1 (en) 2023-12-18 2025-06-19 Illumina, Inc. Hydrogel nanoparticles as labeling scaffold in sequencing
WO2025137222A1 (en) 2023-12-19 2025-06-26 Illumina, Inc. Methylation detection assay
US20250210137A1 (en) 2023-12-20 2025-06-26 Illumina, Inc. Directly determining signal-to-noise-ratio metrics for accelerated convergence in determining nucleotide-base calls and base-call quality
US20250207203A1 (en) 2023-12-20 2025-06-26 10X Genomics, Inc. In situ detection of copy number variations in biological samples
WO2025137647A1 (en) 2023-12-21 2025-06-26 Illumina, Inc. Enhanced mapping and alignment of nucleotide reads utilizing an improved haplotype data structure with allele-variant differences
US20250215493A1 (en) 2023-12-28 2025-07-03 Illumina, Inc. Nucleotides with enzymatically cleavable 3'-o-glycoside blocking groups for sequencing
WO2025144711A1 (en) 2023-12-29 2025-07-03 Illumina, Inc. Tricyclic polymethine dyes for nucleic acid sequencing
WO2025160089A1 (en) 2024-01-26 2025-07-31 Illumina, Inc. Custom multigenome reference construction for improved sequencing analysis of genomic samples
WO2025174774A1 (en) 2024-02-12 2025-08-21 Illumina, Inc. Determining offline corrections for sequence specific errors caused by low complexity nucleotide sequences
WO2025174708A1 (en) 2024-02-13 2025-08-21 Illumina, Inc. Design and method for cross-sequencing platform compatibility of flow cells
WO2025178951A1 (en) 2024-02-22 2025-08-28 Illumina, Inc. Techniques for dynamic range compression grouping in analyte assays
WO2025184234A1 (en) 2024-02-28 2025-09-04 Illumina, Inc. A personalized haplotype database for improved mapping and alignment of nucleotide reads and improved genotype calling
WO2025184226A1 (en) 2024-02-28 2025-09-04 Illumina, Inc. Nucleotides with terminal phosphate capping
WO2025188906A1 (en) 2024-03-08 2025-09-12 Illumina, Inc. Modified adenosine nucleotides
WO2025193747A1 (en) 2024-03-12 2025-09-18 Illumina, Inc. Machine-learning models for ordering and expediting sequencing tasks or corresponding nucleotide-sample slides
WO2025194033A1 (en) 2024-03-15 2025-09-18 10X Genomics, Inc. Systems and methods for covering and sealing an open well
WO2025217057A1 (en) 2024-04-08 2025-10-16 Illumina, Inc. Variant detection using improved sequence data alignments
WO2025221895A1 (en) 2024-04-19 2025-10-23 Illumina, Inc. Water soluble polymer scaffolds for dye labeling
WO2025230914A1 (en) 2024-04-29 2025-11-06 Illumina, Inc. Nucleotides with enzyme-triggered self-immolative linkers for sequencing by synthesis
WO2025240241A1 (en) 2024-05-13 2025-11-20 Illumina, Inc. Modifying sequencing cycles during a sequencing run to meet customized coverage estimations for a target genomic region
WO2025240918A1 (en) 2024-05-17 2025-11-20 10X Genomics, Inc. Systems and methods for generating codebooks
WO2025240924A1 (en) 2024-05-17 2025-11-20 Illumina, Inc. Blind equalization systems for base calling applications
WO2025250794A1 (en) 2024-05-31 2025-12-04 Illumina, Inc. Two-copy allele detection
WO2025250996A2 (en) 2024-05-31 2025-12-04 Illumina, Inc. Call generation and recalibration models for implementing personalized diploid reference haplotypes in genotype calling
WO2025255457A1 (en) 2024-06-07 2025-12-11 10X Genomics, Inc. Compositions and methods for improved on-target spatial profiling

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5756334A (en) 1990-04-26 1998-05-26 New England Biolabs, Inc. Thermostable DNA polymerase from 9°N-7 and methods for producing the same
WO1997039150A1 (en) 1996-04-15 1997-10-23 University Of Southern California Synthesis of fluorophore-labeled dna
EP1218546A4 (de) * 1999-09-30 2005-01-05 New England Biolabs Inc Einbau von modifizierten nukleotiden durch die archaeon-dna-polymerasen und damit zusammenhängende methoden
US8268605B2 (en) * 1999-10-29 2012-09-18 Agilent Technologies, Inc. Compositions and methods utilizing DNA polymerases
US20030228616A1 (en) 1999-10-29 2003-12-11 Stratagene DNA polymerase mutants with reverse transcriptase activity
US6946273B1 (en) 1999-10-29 2005-09-20 Stratagene California Compositions and methods utilizing DNA polymerases
US6329178B1 (en) * 2000-01-14 2001-12-11 University Of Washington DNA polymerase mutant having one or more mutations in the active site
AU2007201277B2 (en) 2000-05-24 2011-10-13 Third Wave Technologies, Inc. Detection of RNA
WO2002010750A2 (en) 2000-07-31 2002-02-07 Maxygen, Inc. Biosensors, reagents and diagnostic applications of directed evolution
EP1395805A4 (de) 2001-06-11 2005-03-09 Illumina Inc Multiplex-detektionsverfahren
DK3002289T3 (en) * 2002-08-23 2018-04-23 Illumina Cambridge Ltd MODIFIED NUCLEOTIDES FOR POLYNUCLEOTIDE SEQUENCE
GB0321306D0 (en) 2003-09-11 2003-10-15 Solexa Ltd Modified polymerases for improved incorporation of nucleotide analogues
US20070048748A1 (en) 2004-09-24 2007-03-01 Li-Cor, Inc. Mutant polymerases for sequencing and genotyping
EP1888743B1 (de) 2005-05-10 2011-08-03 Illumina Cambridge Limited Verbesserte polymerasen

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