ATE467678T1 - Produktion von poxviren mit adhärenten oder nicht adhärenten vogelzellinien - Google Patents

Produktion von poxviren mit adhärenten oder nicht adhärenten vogelzellinien

Info

Publication number
ATE467678T1
ATE467678T1 AT04744255T AT04744255T ATE467678T1 AT E467678 T1 ATE467678 T1 AT E467678T1 AT 04744255 T AT04744255 T AT 04744255T AT 04744255 T AT04744255 T AT 04744255T AT E467678 T1 ATE467678 T1 AT E467678T1
Authority
AT
Austria
Prior art keywords
adherent
poxviruses
production
cell lines
bird cell
Prior art date
Application number
AT04744255T
Other languages
English (en)
Inventor
Fabienne Guehenneux
Bertrand Pain
Original Assignee
Vivalis
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from EP03291813A external-priority patent/EP1500699A1/de
Application filed by Vivalis filed Critical Vivalis
Application granted granted Critical
Publication of ATE467678T1 publication Critical patent/ATE467678T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/04—Antibacterial agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • A61P31/20—Antivirals for DNA viruses
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
    • A61K2039/525—Virus
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
    • A61K2039/525—Virus
    • A61K2039/5256—Virus expressing foreign proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/24011—Poxviridae
    • C12N2710/24111—Orthopoxvirus, e.g. vaccinia virus, variola
    • C12N2710/24141—Use of virus, viral particle or viral elements as a vector
    • C12N2710/24143—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/24011—Poxviridae
    • C12N2710/24111—Orthopoxvirus, e.g. vaccinia virus, variola
    • C12N2710/24151—Methods of production or purification of viral material
    • C12N2710/24152—Methods of production or purification of viral material relating to complementing cells and packaging systems for producing virus or viral particles
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Virology (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • General Chemical & Material Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Oncology (AREA)
  • Molecular Biology (AREA)
  • Communicable Diseases (AREA)
  • Immunology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
AT04744255T 2003-07-22 2004-07-22 Produktion von poxviren mit adhärenten oder nicht adhärenten vogelzellinien ATE467678T1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP03291813A EP1500699A1 (de) 2003-07-22 2003-07-22 Herstellung von Vacciniaviren mit Hilfe adhärenter oder nicht adhärenter Vogelzelllinien
FR0314389 2003-12-09
PCT/IB2004/002621 WO2005007840A1 (en) 2003-07-22 2004-07-22 Production of poxviruses with adherent or non adherent avian cell lines

Publications (1)

Publication Number Publication Date
ATE467678T1 true ATE467678T1 (de) 2010-05-15

Family

ID=34081825

Family Applications (1)

Application Number Title Priority Date Filing Date
AT04744255T ATE467678T1 (de) 2003-07-22 2004-07-22 Produktion von poxviren mit adhärenten oder nicht adhärenten vogelzellinien

Country Status (15)

Country Link
US (3) US7432101B2 (de)
EP (2) EP1646715B1 (de)
JP (2) JP4787156B2 (de)
KR (1) KR101121971B1 (de)
AT (1) ATE467678T1 (de)
AU (1) AU2004257939B2 (de)
CA (1) CA2531565C (de)
DE (1) DE602004027141D1 (de)
DK (1) DK1646715T3 (de)
ES (1) ES2345820T3 (de)
HK (1) HK1091229A1 (de)
PL (1) PL1646715T3 (de)
PT (1) PT1646715E (de)
SI (1) SI1646715T1 (de)
WO (1) WO2005007840A1 (de)

Families Citing this family (50)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7445924B2 (en) * 2000-11-23 2008-11-04 Bavarian Nordic A/S Modified Vaccinia Ankara virus variant and cultivation method
FR2836924B1 (fr) 2002-03-08 2005-01-14 Vivalis Lignees de cellules aviaires utiles pour la production de substances d'interet
PL215169B1 (pl) * 2002-09-05 2013-10-31 Bavarian Nordic As Sposób amplifikacji wirusów, kompozycja zawierajaca pokswirusa uzyskanego tym sposobem oraz jego zastosowanie do otrzymywania szczepionki
PL1646715T3 (pl) * 2003-07-22 2010-10-29 Valneva Wytwarzanie pokswirusów za pomocą linii adherentnych lub nieadherentnych komórek ptasich
EP1528101A1 (de) 2003-11-03 2005-05-04 ProBioGen AG Immortalisierte Vogel-Zelllinien für die Produktion von Viren
FR2884255B1 (fr) * 2005-04-11 2010-11-05 Vivalis Utilisation de lignees de cellules souches aviaires ebx pour la production de vaccin contre la grippe
AU2011253998B2 (en) * 2005-04-11 2014-04-24 Valneva Process of manufacturing viral vaccines in suspension avian embryonic derived stem cell lines
FR2898909A1 (fr) 2006-03-24 2007-09-28 Agronomique Inst Nat Rech Combinaison de marqueurs de cellules aviaires
WO2007135133A1 (en) * 2006-05-19 2007-11-29 Vivalis Avian cell lines derived from primordial germ cells useful for the production of substances of interest
RU2551982C2 (ru) 2006-06-20 2015-06-10 Трансжене С.А. Способ получения поксвирусов и композиции поксвирусов
JP5405302B2 (ja) 2006-08-09 2014-02-05 ビバリス 胚幹細胞を用いたトランスジェニック鳥類の生産方法
CA2680130C (en) * 2007-03-05 2016-01-12 Caridianbct, Inc. Cell expansion system and methods of use
EP1985305A1 (de) * 2007-04-24 2008-10-29 Vivalis Aus Entenembryonen gewonnene Stammzellkulturen zur Herstellung von Impfstoffen gegen Viren
US8357531B2 (en) 2007-07-03 2013-01-22 Transgene S.A. Immortalized avian cell lines
CA2691868C (en) 2007-07-03 2016-12-20 Transgene S.A. Immortalized avian cell lines
PL214884B1 (pl) * 2008-09-29 2013-09-30 Inst Immunologii I Terapii Doswiadczalnej Pan Test ELISA do wykrywania w surowicy bydlecej przeciwcial przeciwko antygenowi powierzchniowemu gp51 BLV
DK2379598T3 (en) 2009-01-19 2015-06-15 Innate Pharma Anti-kir3d antibodies
EP2393922A1 (de) * 2009-02-06 2011-12-14 GlaxoSmithKline Biologicals S.A. Aufreinigung von virus oder viralen antigenen mittels dichtegradienten-ultrazentrifugation
CN102740881A (zh) 2009-05-12 2012-10-17 特兰斯吉恩股份有限公司 正痘病毒产生和纯化方法
WO2012010280A1 (en) 2010-07-20 2012-01-26 Bavarian Nordic A/S Method for harvesting expression products
EP3572501A1 (de) * 2010-09-07 2019-11-27 Technion Research & Development Foundation Limited Neuartige verfahren und kulturmedien zur kultivierung von pluripotenten stammzellen
EP2625263B1 (de) 2010-10-08 2020-03-11 Terumo BCT, Inc. Konfigurierbare verfahren und systeme für züchtung und ernte von zellen in einem hohlfaser-bioreaktorsystem
EP2660316A1 (de) 2012-05-02 2013-11-06 Helmholtz-Zentrum für Infektionsforschung GmbH Vogelzelllinie und deren Verwendung bei der Herstellung eines Proteins
US20140255447A1 (en) * 2013-03-05 2014-09-11 Biomune Company Production of avian embryo cells
WO2015073918A1 (en) 2013-11-16 2015-05-21 Terumo Bct, Inc. Expanding cells in a bioreactor
EP3122866B1 (de) 2014-03-25 2019-11-20 Terumo BCT, Inc. Passives ersetzen von medien
US10077421B2 (en) 2014-04-24 2018-09-18 Terumo Bct, Inc. Measuring flow rate
EP3198006B1 (de) 2014-09-26 2021-03-24 Terumo BCT, Inc. Geplante fütterung
WO2016087457A1 (en) 2014-12-01 2016-06-09 Transgene Sa Stable liquid vaccinia virus formulations
WO2016088243A1 (ja) * 2014-12-05 2016-06-09 株式会社ニコン 判定装置、観察システム、観察方法、そのプログラム、細胞の製造方法、および細胞
WO2017004592A1 (en) 2015-07-02 2017-01-05 Terumo Bct, Inc. Cell growth with mechanical stimuli
CA3023022A1 (en) 2016-05-04 2017-11-09 Transgene Sa Combination therapy with cpg tlr9 ligand
EP3464565A4 (de) 2016-05-25 2020-01-01 Terumo BCT, Inc. Zellexpansion
US11104874B2 (en) 2016-06-07 2021-08-31 Terumo Bct, Inc. Coating a bioreactor
US11685883B2 (en) 2016-06-07 2023-06-27 Terumo Bct, Inc. Methods and systems for coating a cell growth surface
US20190328869A1 (en) 2016-10-10 2019-10-31 Transgene Sa Immunotherapeutic product and mdsc modulator combination therapy
EP3656842A1 (de) 2017-03-31 2020-05-27 Terumo BCT, Inc. Zellexpansion
US11624046B2 (en) 2017-03-31 2023-04-11 Terumo Bct, Inc. Cell expansion
AU2018269319A1 (en) 2017-05-15 2019-11-07 Janssen Vaccines & Prevention B.V. Stable virus-containing composition
EP3624845A1 (de) 2017-05-15 2020-03-25 Janssen Vaccines & Prevention B.V. Stabile virushaltige zusammensetzung
WO2018234506A2 (en) 2017-06-21 2018-12-27 Transgene Sa PERSONALIZED VACCINE
SG11202011280SA (en) 2018-05-30 2020-12-30 Inst Res Biomedicine Engineering b lymphocytes by utilizing endogenous activation-induced cytidine deaminase
JP7437385B2 (ja) 2018-09-06 2024-02-22 バヴァリアン・ノルディック・アクティーゼルスカブ 保管が改善されたポックスウイルス組成物
BR112021009793A2 (pt) 2018-11-23 2021-08-17 Valneva Se produtos alimentícios que compreendem células-tronco aviárias
BR112021014116A2 (pt) * 2019-01-17 2021-09-21 Boehringer Ingelheim Animal Health USA Inc. Meio sem soro para produção de vacinas aviárias e seus usos
EP4117722A1 (de) 2020-03-12 2023-01-18 Bavarian Nordic A/S Zusammensetzungen zur verbesserung der pockenvirusstabilität
US20230242888A1 (en) * 2020-06-22 2023-08-03 Kolon Life Science, Inc. Method for mass-producing vaccinia virus by using suspension cells
EP4178605A1 (de) 2020-07-13 2023-05-17 Transgene Behandlung von immundepression
WO2023213764A1 (en) 2022-05-02 2023-11-09 Transgene Fusion polypeptide comprising an anti-pd-l1 sdab and a member of the tnfsf
WO2023213763A1 (en) 2022-05-02 2023-11-09 Transgene Poxvirus encoding a binding agent comprising an anti- pd-l1 sdab

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5340740A (en) 1992-05-15 1994-08-23 North Carolina State University Method of producing an avian embryonic stem cell culture and the avian embryonic stem cell culture produced by the process
FR2726003B1 (fr) 1994-10-21 2002-10-18 Agronomique Inst Nat Rech Milieu de culture de cellules embryonnaires totipotentes aviaires, procede de culture de ces cellules, et cellules embryonnaires totipotentes aviaires
US5989805A (en) 1995-10-27 1999-11-23 Board Of Trustees Operating Michigan State University Immortal avian cell line to grow avian and animal viruses to produce vaccines
US5672485A (en) 1996-08-13 1997-09-30 Regents Of The University Of Minnesota Immortalized cell lines for virus growth
DE69729839T2 (de) 1996-11-12 2005-07-21 Pfizer Inc. Attenuierter lebender Neospora Impfstoff
CN100463085C (zh) * 2002-02-19 2009-02-18 塞勒吉有限公司 电化学电容器及其制备方法
FR2836924B1 (fr) * 2002-03-08 2005-01-14 Vivalis Lignees de cellules aviaires utiles pour la production de substances d'interet
US20040170646A1 (en) * 2002-12-13 2004-09-02 Aventis Pasteur, Inc. Production of ALVAC on avian embryonic stem cells
PL1646715T3 (pl) * 2003-07-22 2010-10-29 Valneva Wytwarzanie pokswirusów za pomocą linii adherentnych lub nieadherentnych komórek ptasich
EP1528101A1 (de) * 2003-11-03 2005-05-04 ProBioGen AG Immortalisierte Vogel-Zelllinien für die Produktion von Viren

Also Published As

Publication number Publication date
JP5416169B2 (ja) 2014-02-12
CA2531565C (en) 2014-02-11
PL1646715T3 (pl) 2010-10-29
JP2006527995A (ja) 2006-12-14
US20100111999A1 (en) 2010-05-06
US20060233834A1 (en) 2006-10-19
EP1646715A1 (de) 2006-04-19
EP1646715B1 (de) 2010-05-12
CA2531565A1 (en) 2005-01-27
KR20060057577A (ko) 2006-05-26
ES2345820T3 (es) 2010-10-04
HK1091229A1 (en) 2007-01-12
SI1646715T1 (sl) 2010-09-30
JP2011224009A (ja) 2011-11-10
AU2004257939A1 (en) 2005-01-27
DK1646715T3 (da) 2010-08-16
KR101121971B1 (ko) 2012-03-09
US7432101B2 (en) 2008-10-07
US20090239286A1 (en) 2009-09-24
DE602004027141D1 (de) 2010-06-24
AU2004257939B2 (en) 2008-06-05
US7771980B2 (en) 2010-08-10
JP4787156B2 (ja) 2011-10-05
WO2005007840A1 (en) 2005-01-27
PT1646715E (pt) 2010-08-09
EP2143788A1 (de) 2010-01-13

Similar Documents

Publication Publication Date Title
ATE467678T1 (de) Produktion von poxviren mit adhärenten oder nicht adhärenten vogelzellinien
ATE393212T2 (de) Verfahren zur amplifikation von einem poxvirus in serumfreien verhältnissen
DK1483369T3 (da) Fuglecellelinier til fremstiling af stoffer af interesse
WO2010147395A3 (en) Medium composition comprising neuropeptide y for the generation, maintenance, prologned undifferentiated growth of pluripotent stem cells and method of culturing pluripotent stem cell using the same
ATE447010T2 (de) Verfahren zur produktion von viralen antigenen im grossmassstab
AR069956A1 (es) Procesos para cultivar celulas mamiferas secretoras de proteinas sanguineas heterologas
JP2006527995A5 (de)
ATE414144T1 (de) Kulturen von e1-immortalisierten zellen und verfahren zu deren kultivierung zur erhöhung der produktausbeuten davon
DE602005021379D1 (de) System und verfahren zur produktion von antikörpern in der pflanzenzellkultur
BR112018011902A2 (pt) método de fermentação, levedura geneticamente modificada, construto de ácido nucleico, vetor, célula hospedeira, meio de fermentação e utilização da levedura geneticamente modificada
WO2003038076A1 (fr) Cellules mesenchymateuses immortalisees et leur utilisation
CY1111253T1 (el) Μεθοδος για την παραγωγη ιικου υλικου
DE60319599D1 (de) Verfahren zur differenzierung einer mesenchym-stammzelle zu einer nervenzelle sowie die nervenzelle eine neurodegenerative krankheit
CL2008003821A1 (es) Metodo para producir el virus de la estomatitis vesicular (vsv) atenuado en celulas, que comprende: a) proporcionar una celula que comprende un gen g vsv optimizado e inducible, b) inducir la celula a expresar dicho gen, c) infectar la celula con un vsv atenuado, d) hacer crecer dichas celulas, y e) recuperar el vsv atenuado
SG11201806843UA (en) Method for producing activated hepatocyte growth factor (hgf)
ATE366803T1 (de) Hohe zytokinproduktion mit verbesserte zelllebensfähigkeit
ATE400662T1 (de) Herstellung von frostschutzprotein
WO2004085631A3 (en) Canine embryonic stem cells
SG11201804754SA (en) Virus infection model, preparation method therefor, and utilization thereof
CN106591295B (zh) 一种多功能干细胞培养方法及所用培养基
CN203692142U (zh) 组装式社会应激笼
EA201891933A1 (ru) Гифомицетовые биоподстилки, способы их получения и способы их применения
CN205648715U (zh) 一种大型的鲍鱼养殖框
CN204418395U (zh) 一种应用于木结构中的防风扣件
EP4357448A3 (de) Serumfreies medium zur herstellung von vogelimpfstoffen und verwendungen davon

Legal Events

Date Code Title Description
UEP Publication of translation of european patent specification

Ref document number: 1646715

Country of ref document: EP