HK1091229A1 - Production of poxviruses with adherent or non adherent avian cell lines - Google Patents

Production of poxviruses with adherent or non adherent avian cell lines

Info

Publication number
HK1091229A1
HK1091229A1 HK06111514.8A HK06111514A HK1091229A1 HK 1091229 A1 HK1091229 A1 HK 1091229A1 HK 06111514 A HK06111514 A HK 06111514A HK 1091229 A1 HK1091229 A1 HK 1091229A1
Authority
HK
Hong Kong
Prior art keywords
adherent
poxviruses
production
cell lines
avian cell
Prior art date
Application number
HK06111514.8A
Other languages
English (en)
Inventor
Fabienne Guehenneux
Bertrand Pain
Original Assignee
Vivalis
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from EP03291813A external-priority patent/EP1500699A1/en
Application filed by Vivalis filed Critical Vivalis
Publication of HK1091229A1 publication Critical patent/HK1091229A1/xx

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/04—Antibacterial agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • A61P31/20—Antivirals for DNA viruses
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
    • A61K2039/525—Virus
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
    • A61K2039/525—Virus
    • A61K2039/5256—Virus expressing foreign proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/24011—Poxviridae
    • C12N2710/24111—Orthopoxvirus, e.g. vaccinia virus, variola
    • C12N2710/24141—Use of virus, viral particle or viral elements as a vector
    • C12N2710/24143—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/24011—Poxviridae
    • C12N2710/24111—Orthopoxvirus, e.g. vaccinia virus, variola
    • C12N2710/24151—Methods of production or purification of viral material
    • C12N2710/24152—Methods of production or purification of viral material relating to complementing cells and packaging systems for producing virus or viral particles
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Virology (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • General Chemical & Material Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Oncology (AREA)
  • Molecular Biology (AREA)
  • Communicable Diseases (AREA)
  • Immunology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
HK06111514.8A 2003-07-22 2006-10-19 Production of poxviruses with adherent or non adherent avian cell lines HK1091229A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP03291813A EP1500699A1 (en) 2003-07-22 2003-07-22 Production of vaccinia virus with adherent or non adherent avian cell lines
FR0314389 2003-12-09
PCT/IB2004/002621 WO2005007840A1 (en) 2003-07-22 2004-07-22 Production of poxviruses with adherent or non adherent avian cell lines

Publications (1)

Publication Number Publication Date
HK1091229A1 true HK1091229A1 (en) 2007-01-12

Family

ID=34081825

Family Applications (1)

Application Number Title Priority Date Filing Date
HK06111514.8A HK1091229A1 (en) 2003-07-22 2006-10-19 Production of poxviruses with adherent or non adherent avian cell lines

Country Status (15)

Country Link
US (3) US7432101B2 (xx)
EP (2) EP1646715B1 (xx)
JP (2) JP4787156B2 (xx)
KR (1) KR101121971B1 (xx)
AT (1) ATE467678T1 (xx)
AU (1) AU2004257939B2 (xx)
CA (1) CA2531565C (xx)
DE (1) DE602004027141D1 (xx)
DK (1) DK1646715T3 (xx)
ES (1) ES2345820T3 (xx)
HK (1) HK1091229A1 (xx)
PL (1) PL1646715T3 (xx)
PT (1) PT1646715E (xx)
SI (1) SI1646715T1 (xx)
WO (1) WO2005007840A1 (xx)

Families Citing this family (50)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7445924B2 (en) * 2000-11-23 2008-11-04 Bavarian Nordic A/S Modified Vaccinia Ankara virus variant and cultivation method
FR2836924B1 (fr) 2002-03-08 2005-01-14 Vivalis Lignees de cellules aviaires utiles pour la production de substances d'interet
NZ538575A (en) * 2002-09-05 2007-03-30 Bavarian Nordic As Method for the amplification of a virus wherein primary avian cells are cultivated in a serum-free medium comprising growth factors and attachment factors
CA2531565C (en) * 2003-07-22 2014-02-11 Vivalis Production of poxviruses with adherent or non adherent avian cell lines
EP1528101A1 (en) 2003-11-03 2005-05-04 ProBioGen AG Immortalized avian cell lines for virus production
FR2884255B1 (fr) 2005-04-11 2010-11-05 Vivalis Utilisation de lignees de cellules souches aviaires ebx pour la production de vaccin contre la grippe
AU2011253998B2 (en) * 2005-04-11 2014-04-24 Valneva Process of manufacturing viral vaccines in suspension avian embryonic derived stem cell lines
FR2898909A1 (fr) 2006-03-24 2007-09-28 Agronomique Inst Nat Rech Combinaison de marqueurs de cellules aviaires
WO2007135133A1 (en) * 2006-05-19 2007-11-29 Vivalis Avian cell lines derived from primordial germ cells useful for the production of substances of interest
NZ573437A (en) 2006-06-20 2012-02-24 Transgene Sa Process for producing poxviruses and poxvirus compositions
NZ574678A (en) 2006-08-09 2012-03-30 Vivalis Method of production of transgenic avian using embryonic stem cells
US8309347B2 (en) * 2007-03-05 2012-11-13 Terumo Bct, Inc. Cell expansion system and methods of use
EP1985305A1 (en) * 2007-04-24 2008-10-29 Vivalis Duck embryonic derived stem cell lines for the production of viral vaccines
US8357531B2 (en) 2007-07-03 2013-01-22 Transgene S.A. Immortalized avian cell lines
KR101528379B1 (ko) 2007-07-03 2015-06-16 트랜스진 에스.에이. 불멸화된 조류 세포주들
PL214884B1 (pl) * 2008-09-29 2013-09-30 Inst Immunologii I Terapii Doswiadczalnej Pan Test ELISA do wykrywania w surowicy bydlecej przeciwcial przeciwko antygenowi powierzchniowemu gp51 BLV
WO2010081890A1 (en) 2009-01-19 2010-07-22 Innate Pharma Anti-kir3d antibodies
CA2750055A1 (en) * 2009-02-06 2010-08-12 Glaxosmithkline Biologicals S.A. Method for purifying viruses using a density gradient
US8609392B2 (en) 2009-05-12 2013-12-17 Transgene S.A. Method for orthopoxvirus production and purification
NZ603620A (en) 2010-07-20 2015-01-30 Bavarian Nordic As Method for harvesting expression products
SG188411A1 (en) * 2010-09-07 2013-04-30 Technion Res & Dev Foundation Novel methods and culture media for culturing pluripotent stem cells
WO2012048275A2 (en) 2010-10-08 2012-04-12 Caridianbct, Inc. Configurable methods and systems of growing and harvesting cells in a hollow fiber bioreactor system
EP2660316A1 (en) 2012-05-02 2013-11-06 Helmholtz-Zentrum für Infektionsforschung GmbH Avian cell line and its use in production of protein
US20140255447A1 (en) * 2013-03-05 2014-09-11 Biomune Company Production of avian embryo cells
JP6612227B2 (ja) 2013-11-16 2019-11-27 テルモ ビーシーティー、インコーポレーテッド バイオリアクターにおける細胞増殖
EP3122866B1 (en) 2014-03-25 2019-11-20 Terumo BCT, Inc. Passive replacement of media
US10077421B2 (en) 2014-04-24 2018-09-18 Terumo Bct, Inc. Measuring flow rate
JP6830059B2 (ja) 2014-09-26 2021-02-17 テルモ ビーシーティー、インコーポレーテッド スケジュール化された細胞フィーディング
US10517943B2 (en) 2014-12-01 2019-12-31 Transgene S.A. Stable liquid vaccinia virus formulations
WO2016088243A1 (ja) * 2014-12-05 2016-06-09 株式会社ニコン 判定装置、観察システム、観察方法、そのプログラム、細胞の製造方法、および細胞
WO2017004592A1 (en) 2015-07-02 2017-01-05 Terumo Bct, Inc. Cell growth with mechanical stimuli
CA3023022A1 (en) 2016-05-04 2017-11-09 Transgene Sa Combination therapy with cpg tlr9 ligand
JP7034949B2 (ja) 2016-05-25 2022-03-14 テルモ ビーシーティー、インコーポレーテッド 細胞の増殖
US11685883B2 (en) 2016-06-07 2023-06-27 Terumo Bct, Inc. Methods and systems for coating a cell growth surface
US11104874B2 (en) 2016-06-07 2021-08-31 Terumo Bct, Inc. Coating a bioreactor
WO2018069316A2 (en) 2016-10-10 2018-04-19 Transgene Sa Immunotherapeutic product and mdsc modulator combination therapy
US11624046B2 (en) 2017-03-31 2023-04-11 Terumo Bct, Inc. Cell expansion
CN110612344B (zh) 2017-03-31 2023-09-12 泰尔茂比司特公司 细胞扩增
WO2018211419A1 (en) 2017-05-15 2018-11-22 Janssen Vaccines & Prevention B.V. Stable virus-containing composition
EP3624851A1 (en) 2017-05-15 2020-03-25 Janssen Vaccines & Prevention B.V. Stable virus-containing composition
KR20240113607A (ko) 2017-06-21 2024-07-22 트랜스진 개인 맞춤형 백신
JP2021525517A (ja) 2018-05-30 2021-09-27 インスティテュート・フォー・リサーチ・イン・バイオメディシンＩｎｓｔｉｔｕｔｅ Ｆｏｒ Ｒｅｓｅａｒｃｈ Ｉｎ Ｂｉｏｍｅｄｉｃｉｎｅ 内在性活性化誘導シチジンデアミナーゼを利用することによるｂリンパ球のエンジニアリング
WO2020049151A1 (en) 2018-09-06 2020-03-12 Bavarian Nordic A/S Storage improved poxvirus compositions
SG11202104869SA (en) 2018-11-23 2021-06-29 Valneva Se Food products comprising avian stem cells
MX2021006115A (es) * 2019-01-17 2021-07-07 Boehringer Ingelheim Animal Health Usa Inc Medio libre de suero para produccion de vacuna aviar y usos del mismo.
MX2022011071A (es) 2020-03-12 2022-09-23 Bavarian Nordic As Composiciones que mejoran la estabilidad del poxvirus.
CN115943206A (zh) * 2020-06-22 2023-04-07 可隆生命科学株式会社 利用悬浮细胞大量生产痘苗病毒的方法
CA3189238A1 (en) 2020-07-13 2022-01-20 Transgene Treatment of immune depression
WO2023213763A1 (en) 2022-05-02 2023-11-09 Transgene Poxvirus encoding a binding agent comprising an anti- pd-l1 sdab
WO2023213764A1 (en) 2022-05-02 2023-11-09 Transgene Fusion polypeptide comprising an anti-pd-l1 sdab and a member of the tnfsf

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5340740A (en) 1992-05-15 1994-08-23 North Carolina State University Method of producing an avian embryonic stem cell culture and the avian embryonic stem cell culture produced by the process
FR2726003B1 (fr) 1994-10-21 2002-10-18 Agronomique Inst Nat Rech Milieu de culture de cellules embryonnaires totipotentes aviaires, procede de culture de ces cellules, et cellules embryonnaires totipotentes aviaires
US5989805A (en) * 1995-10-27 1999-11-23 Board Of Trustees Operating Michigan State University Immortal avian cell line to grow avian and animal viruses to produce vaccines
US5672485A (en) 1996-08-13 1997-09-30 Regents Of The University Of Minnesota Immortalized cell lines for virus growth
PT841392E (pt) 1996-11-12 2004-11-30 Univ Auburn Vacina viva atenuada de neospora
JP2005518662A (ja) * 2002-02-19 2005-06-23 セラジー リミテッド 電気化学コンデンサー及びその製造方法
FR2836924B1 (fr) 2002-03-08 2005-01-14 Vivalis Lignees de cellules aviaires utiles pour la production de substances d'interet
EP1572985A4 (en) * 2002-12-13 2008-03-19 Aventis Pasteur Inc ALVAC PRODUCTION ON AVIAN STEM CELLS
CA2531565C (en) * 2003-07-22 2014-02-11 Vivalis Production of poxviruses with adherent or non adherent avian cell lines
EP1528101A1 (en) * 2003-11-03 2005-05-04 ProBioGen AG Immortalized avian cell lines for virus production

Also Published As

Publication number Publication date
US7771980B2 (en) 2010-08-10
EP2143788A1 (en) 2010-01-13
DE602004027141D1 (de) 2010-06-24
SI1646715T1 (sl) 2010-09-30
ATE467678T1 (de) 2010-05-15
PT1646715E (pt) 2010-08-09
JP2006527995A (ja) 2006-12-14
AU2004257939B2 (en) 2008-06-05
JP4787156B2 (ja) 2011-10-05
WO2005007840A1 (en) 2005-01-27
US20060233834A1 (en) 2006-10-19
US7432101B2 (en) 2008-10-07
EP1646715A1 (en) 2006-04-19
EP1646715B1 (en) 2010-05-12
AU2004257939A1 (en) 2005-01-27
CA2531565A1 (en) 2005-01-27
CA2531565C (en) 2014-02-11
ES2345820T3 (es) 2010-10-04
PL1646715T3 (pl) 2010-10-29
JP5416169B2 (ja) 2014-02-12
DK1646715T3 (da) 2010-08-16
KR20060057577A (ko) 2006-05-26
KR101121971B1 (ko) 2012-03-09
JP2011224009A (ja) 2011-11-10
US20100111999A1 (en) 2010-05-06
US20090239286A1 (en) 2009-09-24

Similar Documents

Publication Publication Date Title
HK1091229A1 (en) Production of poxviruses with adherent or non adherent avian cell lines
WO2009077134A3 (en) Use of rna for reprogramming somatic cells
WO2010147395A3 (en) Medium composition comprising neuropeptide y for the generation, maintenance, prologned undifferentiated growth of pluripotent stem cells and method of culturing pluripotent stem cell using the same
WO2008036447A3 (en) Pluripotent stem cell culture
MX2007005132A (es) Medios libres de proteina animal para cultivo de celulas.
JP2008212150A5 (xx)
WO2010006312A3 (en) Methods and compositions for producing carbon-based products in microorganisms
MY157524A (en) Culturing embryonic stem cells, embryonic stem-like cells, or induced pluripotent stem cells with a muc1 or muc1* ligand
HK1080507A1 (en) Method for the cultivation of primary cells and for the amplification of viruses under serum free conditions
MX2008007654A (es) Factor de reprogramacion nuclear.
GB2432846B (en) Medium and culture of embryonic stem cells
JP2006527995A5 (xx)
WO2006029198A3 (en) Culturing human embryonic stem cells
DK1483369T3 (da) Fuglecellelinier til fremstiling af stoffer af interesse
WO2004055155A3 (en) Methods of preparing feeder cells-free, xeno-free human embryonic stem cells and stem cell cultures prepared using same
MXPA05009096A (es) Metodo de cultivo celular libre de animal.
GB2449772A (en) Large scale production of stem cells
WO2003023018A3 (en) A method for isolating, culturing and differentiating intestinal stem cells for therapeutic use
WO2007070315A3 (en) Improved production of glycoproteins using manganese
WO2007115337A8 (en) Chondrocyte differentiation from human embryonic stem cells and their use in tissue engineering
WO2003038076A1 (fr) Cellules mesenchymateuses immortalisees et leur utilisation
WO2007016366A3 (en) Defined culture conditions of human embryonic stem cells
WO2012014207A3 (en) Method for generating induced pluripotent stem cells from keratinocytes derived from plucked hair follicles
WO2011160128A3 (en) Cardiomyocyte medium with dialyzed serum
WO2017213469A3 (ko) 남조류 스피룰리나 추출물을 함유하는 세포 배양액, 이의 제조방법, 및 이를 이용한 세포의 배양방법

Legal Events

Date Code Title Description
PC Patent ceased (i.e. patent has lapsed due to the failure to pay the renewal fee)

Effective date: 20160722