ATE463580T1 - Verfahren zur herstellung von rekombinantem protein und fusionsprotein - Google Patents
Verfahren zur herstellung von rekombinantem protein und fusionsproteinInfo
- Publication number
- ATE463580T1 ATE463580T1 AT01272326T AT01272326T ATE463580T1 AT E463580 T1 ATE463580 T1 AT E463580T1 AT 01272326 T AT01272326 T AT 01272326T AT 01272326 T AT01272326 T AT 01272326T AT E463580 T1 ATE463580 T1 AT E463580T1
- Authority
- AT
- Austria
- Prior art keywords
- protein
- desired protein
- fusion protein
- proteins
- linked
- Prior art date
Links
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 title abstract 3
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 title abstract 3
- 238000004519 manufacturing process Methods 0.000 title 1
- 108090000623 proteins and genes Proteins 0.000 abstract 10
- 102000004169 proteins and genes Human genes 0.000 abstract 7
- 108050001186 Chaperonin Cpn60 Proteins 0.000 abstract 4
- 102000052603 Chaperonins Human genes 0.000 abstract 4
- 102000037865 fusion proteins Human genes 0.000 abstract 2
- 108020001507 fusion proteins Proteins 0.000 abstract 2
- 102000002812 Heat-Shock Proteins Human genes 0.000 abstract 1
- 108010004889 Heat-Shock Proteins Proteins 0.000 abstract 1
- 108010006519 Molecular Chaperones Proteins 0.000 abstract 1
- 102000005431 Molecular Chaperones Human genes 0.000 abstract 1
- 102000035195 Peptidases Human genes 0.000 abstract 1
- 108091005804 Peptidases Proteins 0.000 abstract 1
- 239000004365 Protease Substances 0.000 abstract 1
- 108010010427 Thermosomes Proteins 0.000 abstract 1
- 230000015572 biosynthetic process Effects 0.000 abstract 1
- 238000000354 decomposition reaction Methods 0.000 abstract 1
- 210000003000 inclusion body Anatomy 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
- 108090000765 processed proteins & peptides Proteins 0.000 abstract 1
- 230000002194 synthesizing effect Effects 0.000 abstract 1
- 230000001988 toxicity Effects 0.000 abstract 1
- 231100000419 toxicity Toxicity 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K19/00—Hybrid peptides, i.e. peptides covalently bound to nucleic acids, or non-covalently bound protein-protein complexes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/62—DNA sequences coding for fusion proteins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
- C07K2319/21—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a His-tag
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/35—Fusion polypeptide containing a fusion for enhanced stability/folding during expression, e.g. fusions with chaperones or thioredoxin
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/40—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
- C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
- C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Medicinal Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2000395740 | 2000-12-26 | ||
| PCT/JP2001/011438 WO2002052029A1 (fr) | 2000-12-26 | 2001-12-26 | Methode permettant de produire une proteine de recombinaison et une proteine fusionnee |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE463580T1 true ATE463580T1 (de) | 2010-04-15 |
Family
ID=18861149
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT01272326T ATE463580T1 (de) | 2000-12-26 | 2001-12-26 | Verfahren zur herstellung von rekombinantem protein und fusionsprotein |
Country Status (9)
| Country | Link |
|---|---|
| US (2) | US7276355B2 (de) |
| EP (1) | EP1354959B1 (de) |
| JP (1) | JP4038555B2 (de) |
| KR (1) | KR100892889B1 (de) |
| AT (1) | ATE463580T1 (de) |
| AU (1) | AU2002217505B2 (de) |
| CA (1) | CA2432716A1 (de) |
| DE (1) | DE60141769D1 (de) |
| WO (1) | WO2002052029A1 (de) |
Families Citing this family (21)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP4838957B2 (ja) | 2001-05-02 | 2011-12-14 | 独立行政法人理化学研究所 | 無細胞タンパク質合成系を用いたタンパク質の製造方法 |
| CA2486913A1 (en) | 2002-05-22 | 2003-11-27 | Riken | Process for producing protein in cell-free protein synthesis system using thioredoxin fused protein expression vector |
| JP4441170B2 (ja) | 2002-11-28 | 2010-03-31 | 独立行政法人理化学研究所 | Ｓ１２リボソームタンパク質に変異を有する大腸菌細胞抽出液及びそれを用いる無細胞系によるタンパク質の製造方法 |
| WO2004092221A1 (ja) * | 2003-04-18 | 2004-10-28 | Sekisui Chemical Co. Ltd. | 免疫原及び免疫用組成物、並びにそれらを使用する抗体の製造方法 |
| WO2004097018A1 (ja) * | 2003-04-28 | 2004-11-11 | Sekisui Chemical Co.,Ltd. | 組換え抗体の製造方法 |
| WO2004096860A1 (ja) * | 2003-04-28 | 2004-11-11 | Sekisui Chemical Co., Ltd. | 目的蛋白質の製造方法、融合蛋白質及びその遺伝子、インテインの部分配列蛋白質及びその遺伝子、発現ベクター、並びに形質転換体 |
| US20070059794A1 (en) * | 2003-04-28 | 2007-03-15 | Sekisui Chemical Company Limited | Chaperonin-target protein complex, method of producing the same, method of stabilizing target protein, method of immobilizing target protein, method of analyzing the structure of target protein, sustained-release formulation, and method of producing antibody against target protein |
| JP2005161694A (ja) * | 2003-12-03 | 2005-06-23 | Fuji Photo Film Co Ltd | 露光装置 |
| KR100484653B1 (ko) | 2004-05-06 | 2005-04-20 | 주식회사 대웅 | 원핵세포에서 활성형의 가용성 단백질을 제조하는 방법 및 이를 위한 폴리시스트론 벡터 |
| AU2005292852B2 (en) * | 2004-10-15 | 2011-02-03 | Joe Chiba | Method of immunizing animal, composition for immunization, method of producing antibody, method of producing hybridoma and method of producing monoclonal antibody |
| JP4868731B2 (ja) | 2004-11-17 | 2012-02-01 | 独立行政法人理化学研究所 | 哺乳動物培養細胞由来の無細胞タンパク質合成システム |
| JP4787488B2 (ja) | 2004-11-19 | 2011-10-05 | 独立行政法人理化学研究所 | 直鎖状鋳型ｄｎａを用いた無細胞タンパク質合成方法及びそのための細胞抽出液 |
| US8226976B2 (en) | 2007-11-05 | 2012-07-24 | Riken | Method of using cell-free protein synthesis to produce a membrane protein |
| US20100137563A1 (en) | 2008-12-03 | 2010-06-03 | Northwestern University | Cysteine Protease Autoprocessing of Fusion Proteins |
| JP2010246522A (ja) * | 2009-03-27 | 2010-11-04 | Sekisui Chem Co Ltd | 融合タンパク質、並びに、遺伝子 |
| US8067201B2 (en) * | 2009-04-17 | 2011-11-29 | Bristol-Myers Squibb Company | Methods for protein refolding |
| JP2012120507A (ja) * | 2010-12-10 | 2012-06-28 | Sekisui Chem Co Ltd | 組換え真核細胞、並びに、細胞内シグナル伝達抑制方法 |
| AR086250A1 (es) | 2011-05-05 | 2013-11-27 | Hoffmann La Roche | Polipeptido de fusion presentador de una secuencia de aminoacidos y utilizacion del mismo |
| US11618777B2 (en) | 2015-07-31 | 2023-04-04 | Shigeyuki Yokoyama | Method of manufacturing membrane protein and utilization thereof |
| KR102185312B1 (ko) * | 2017-10-25 | 2020-12-02 | 재단법인 지능형 바이오 시스템 설계 및 합성 연구단 | 목적 단백질의 가용화용 조성물 및 이의 용도 |
| WO2024173386A2 (en) * | 2023-02-13 | 2024-08-22 | National Resilience, Inc. | Cell-free expression vectors and methods for improved protein production |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH07504561A (ja) * | 1991-12-20 | 1995-05-25 | ノボ ノルディスク アクティーゼルスカブ | リパーゼの製造のための方法 |
| FI109361B (fi) | 1999-05-04 | 2002-07-15 | Korpela Timo | Mikrobinen proteiinin ilmentämisjärjestelmä |
| GB9913437D0 (en) * | 1999-06-09 | 1999-08-11 | Medical Res Council | Fusion proteins |
| AU6124201A (en) * | 2000-05-03 | 2001-11-12 | Expressive Constructs Inc | A method and device for improving protein stability and solubility |
-
2001
- 2001-12-26 KR KR1020037008657A patent/KR100892889B1/ko not_active Expired - Fee Related
- 2001-12-26 EP EP01272326A patent/EP1354959B1/de not_active Expired - Lifetime
- 2001-12-26 WO PCT/JP2001/011438 patent/WO2002052029A1/ja not_active Ceased
- 2001-12-26 JP JP2002553508A patent/JP4038555B2/ja not_active Expired - Fee Related
- 2001-12-26 CA CA002432716A patent/CA2432716A1/en not_active Abandoned
- 2001-12-26 US US10/451,883 patent/US7276355B2/en not_active Expired - Fee Related
- 2001-12-26 AU AU2002217505A patent/AU2002217505B2/en not_active Ceased
- 2001-12-26 DE DE60141769T patent/DE60141769D1/de not_active Expired - Lifetime
- 2001-12-26 AT AT01272326T patent/ATE463580T1/de not_active IP Right Cessation
-
2007
- 2007-08-13 US US11/837,795 patent/US7608424B2/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| EP1354959B1 (de) | 2010-04-07 |
| WO2002052029A9 (fr) | 2003-04-24 |
| EP1354959A4 (de) | 2004-09-15 |
| US20080081361A1 (en) | 2008-04-03 |
| JPWO2002052029A1 (ja) | 2004-04-30 |
| WO2002052029A1 (fr) | 2002-07-04 |
| KR100892889B1 (ko) | 2009-04-15 |
| DE60141769D1 (de) | 2010-05-20 |
| JP4038555B2 (ja) | 2008-01-30 |
| CA2432716A1 (en) | 2002-07-04 |
| US7608424B2 (en) | 2009-10-27 |
| US20040146969A1 (en) | 2004-07-29 |
| AU2002217505B2 (en) | 2006-03-02 |
| KR20030065574A (ko) | 2003-08-06 |
| EP1354959A1 (de) | 2003-10-22 |
| US7276355B2 (en) | 2007-10-02 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |