ATE445697T1 - Verfahren zur ausführung einer lyse säurefester bakterien und verfahren zur durchführung einer genamplifikation oder eines gennachweises damit - Google Patents

Verfahren zur ausführung einer lyse säurefester bakterien und verfahren zur durchführung einer genamplifikation oder eines gennachweises damit

Info

Publication number
ATE445697T1
ATE445697T1 AT03730537T AT03730537T ATE445697T1 AT E445697 T1 ATE445697 T1 AT E445697T1 AT 03730537 T AT03730537 T AT 03730537T AT 03730537 T AT03730537 T AT 03730537T AT E445697 T1 ATE445697 T1 AT E445697T1
Authority
AT
Austria
Prior art keywords
acid
lysis
liquid
fast bacteria
heating
Prior art date
Application number
AT03730537T
Other languages
English (en)
Inventor
Tatsuo Kamata
Yuji Izumizawa
Original Assignee
Arkray Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Arkray Inc filed Critical Arkray Inc
Application granted granted Critical
Publication of ATE445697T1 publication Critical patent/ATE445697T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/06—Lysis of microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measurement Of Radiation (AREA)
  • Amplifiers (AREA)
AT03730537T 2002-05-21 2003-05-21 Verfahren zur ausführung einer lyse säurefester bakterien und verfahren zur durchführung einer genamplifikation oder eines gennachweises damit ATE445697T1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2002146823 2002-05-21
JP2002183461 2002-06-24
PCT/JP2003/006321 WO2003097816A1 (fr) 2002-05-21 2003-05-21 Procede permettant d'effectuer la lyse d'une bacterie resistante aux acides et procede permettant de realiser une amplification ou une detection de gene

Publications (1)

Publication Number Publication Date
ATE445697T1 true ATE445697T1 (de) 2009-10-15

Family

ID=29552327

Family Applications (1)

Application Number Title Priority Date Filing Date
AT03730537T ATE445697T1 (de) 2002-05-21 2003-05-21 Verfahren zur ausführung einer lyse säurefester bakterien und verfahren zur durchführung einer genamplifikation oder eines gennachweises damit

Country Status (9)

Country Link
US (2) US20050181363A1 (de)
EP (1) EP1506995B1 (de)
JP (1) JP3910198B2 (de)
KR (1) KR100633808B1 (de)
CN (1) CN1656211B (de)
AT (1) ATE445697T1 (de)
AU (1) AU2003242353A1 (de)
DE (1) DE60329676D1 (de)
WO (1) WO2003097816A1 (de)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7482116B2 (en) 2002-06-07 2009-01-27 Dna Genotek Inc. Compositions and methods for obtaining nucleic acids from sputum
US20100055082A1 (en) * 2008-09-04 2010-03-04 Jacques Alain Bauer Immunomodulatory extracts from lactobacillus bacteria and methods of manufacturing and use thereof
US10190152B2 (en) * 2009-09-03 2019-01-29 Becton, Dickinson And Company Methods and compositions for direct chemical lysis
JP5568968B2 (ja) * 2009-11-30 2014-08-13 東洋紡株式会社 抗酸菌の溶菌方法
JP5714291B2 (ja) * 2010-10-15 2015-05-07 独立行政法人農業・食品産業技術総合研究機構 抗酸菌ｄｎａの抽出精製法
KR20140040239A (ko) 2011-06-19 2014-04-02 아보젠, 인크. 샘플 수집을 위한 장치, 용액 및 방법
KR101370140B1 (ko) * 2012-06-08 2014-03-04 포항공과대학교 산학협력단 항산균 검출장치 및 그 검출방법
CN106255753B (zh) 2014-03-07 2020-11-06 Dna吉诺特克股份有限公司 稳定化生物学样本中的核酸的组合物和方法

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU664050B2 (en) * 1991-12-18 1995-11-02 Becton Dickinson & Company Process for lysing mycobacteria
CA2176496C (en) * 1993-11-29 1999-09-28 Kathleen A. Clark Method for extracting nucleic acids from a wide range of organisms
US5712095A (en) * 1994-06-16 1998-01-27 Becton Dickinson And Company Rapid and sensitive detection of antibiotic-resistant mycobacteria using oligonucleotide probes specific for ribosomal RNA precursors
JPH09178752A (ja) 1995-12-26 1997-07-11 Lion Corp 微生物の検出方法及び検出用検査セット
JP3093164B2 (ja) * 1997-05-07 2000-10-03 三光純薬株式会社 抗酸菌の細胞溶解方法及び核酸の抽出方法
CA2319571C (en) 1998-02-06 2007-09-11 Japan Bcg Laboratory Methods and compositions for detection and diagnosis of infectious diseases
JP3793658B2 (ja) * 1998-10-19 2006-07-05 株式会社日立製作所 生体試料処理装置

Also Published As

Publication number Publication date
CN1656211B (zh) 2010-04-28
AU2003242353A1 (en) 2003-12-02
EP1506995A4 (de) 2005-06-22
KR20040101234A (ko) 2004-12-02
US20050181363A1 (en) 2005-08-18
JP3910198B2 (ja) 2007-04-25
DE60329676D1 (de) 2009-11-26
US7935483B2 (en) 2011-05-03
KR100633808B1 (ko) 2006-10-13
CN1656211A (zh) 2005-08-17
EP1506995B1 (de) 2009-10-14
WO2003097816A1 (fr) 2003-11-27
US20070178508A1 (en) 2007-08-02
EP1506995A1 (de) 2005-02-16
JPWO2003097816A1 (ja) 2005-09-15

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