ATE156861T1 - Verfahren zur extraktion von intrazellulären komponenten - Google Patents

Verfahren zur extraktion von intrazellulären komponenten

Info

Publication number
ATE156861T1
ATE156861T1 AT92902694T AT92902694T ATE156861T1 AT E156861 T1 ATE156861 T1 AT E156861T1 AT 92902694 T AT92902694 T AT 92902694T AT 92902694 T AT92902694 T AT 92902694T AT E156861 T1 ATE156861 T1 AT E156861T1
Authority
AT
Austria
Prior art keywords
extractant
solution
intracellular components
cyclodextrin
neutralization
Prior art date
Application number
AT92902694T
Other languages
English (en)
Inventor
Arne Lundin
John George Anson
Original Assignee
Merck Patent Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Merck Patent Gmbh filed Critical Merck Patent Gmbh
Application granted granted Critical
Publication of ATE156861T1 publication Critical patent/ATE156861T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/66—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving luciferase
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/81—Packaged device or kit
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/10—Composition for standardization, calibration, simulation, stabilization, preparation or preservation; processes of use in preparation for chemical testing
    • Y10T436/107497—Preparation composition [e.g., lysing or precipitation, etc.]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines Containing Plant Substances (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
AT92902694T 1991-01-10 1992-01-10 Verfahren zur extraktion von intrazellulären komponenten ATE156861T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB919100551A GB9100551D0 (en) 1991-01-10 1991-01-10 Method and reagent for eliminating analytical interference in enzymatic analysis from substances used for extraction of intracellular metabolites

Publications (1)

Publication Number Publication Date
ATE156861T1 true ATE156861T1 (de) 1997-08-15

Family

ID=10688253

Family Applications (1)

Application Number Title Priority Date Filing Date
AT92902694T ATE156861T1 (de) 1991-01-10 1992-01-10 Verfahren zur extraktion von intrazellulären komponenten

Country Status (9)

Country Link
US (1) US5558986A (de)
EP (1) EP0566625B1 (de)
JP (1) JP3140057B2 (de)
AT (1) ATE156861T1 (de)
DE (1) DE69221604T2 (de)
DK (1) DK0566625T3 (de)
ES (1) ES2106170T3 (de)
GB (1) GB9100551D0 (de)
WO (1) WO1992012253A1 (de)

Families Citing this family (33)

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US5705345A (en) * 1991-01-10 1998-01-06 Amersham International Plc Methods and kits for preparing nucleic acids using cyclodextrin
US5538870A (en) * 1994-09-21 1996-07-23 Boehringer Mannheim Corporation Method for preparing nucleic acids for analysis and kits useful therefor
JP3118528B2 (ja) 1996-01-26 2000-12-18 東邦レーヨン株式会社 ステアリングホイールおよびその製造方法
US5905029A (en) * 1997-02-19 1999-05-18 Fritz Berthold Method for rapid hygiene testing
US5916802A (en) * 1997-02-19 1999-06-29 Fritz Berthold Device for measuring ATP
DE69831522T2 (de) 1997-03-03 2006-06-14 Amersham Biosciences Uk Ltd In-situ Zellextraktion und Assay-Verfahren
JP3773151B2 (ja) * 1997-12-03 2006-05-10 キッコーマン株式会社 細胞内成分の分析法
JP3749628B2 (ja) 1997-12-26 2006-03-01 キッコーマン株式会社 ルシフェラーゼおよびそれを用いる細胞内ａｔｐの測定法
EP1053357B1 (de) * 1998-02-02 2008-05-21 QIAGEN North American Holdings, Inc. Verfahren zur isolierung, amplifizierung und charakterisierung von dns
WO1999041609A1 (en) * 1998-02-16 1999-08-19 Amersham Pharmacia Biotech Uk Limited Method for measurement of total analyte
US20050089918A1 (en) * 1998-02-23 2005-04-28 Amersham Biosciences Uk Limited In-situ cell extraction and assay method
DE19838429C2 (de) * 1998-08-24 2001-07-26 Fraunhofer Ges Forschung Anordnung zum schnellen Nachweis von mikrobiologischen oder biochemischen Stoffen
NL1010224C2 (nl) * 1998-09-30 2000-03-31 Packard Biosciene B V Werkwijze voor het detecteren van ATP.
US6653147B2 (en) 2000-03-31 2003-11-25 Neogen Corporation Apparatus and method for chemiluminescent assays
US6927851B2 (en) 2000-03-31 2005-08-09 Neogen Corporation Methods and apparatus to improve the sensitivity and reproducibility of bioluminescent analytical methods
US6660489B2 (en) * 2000-12-01 2003-12-09 Becton, Dickinson And Company ATP extraction method
US7083911B2 (en) * 2001-02-16 2006-08-01 Promega Corporation Method for detection of ATP
AU2002331374A1 (en) * 2001-09-11 2003-03-24 Merck Patent Gmbh Lateral flow test format for enzyme assays
EP1446499A2 (de) * 2001-11-16 2004-08-18 Pierce Biotechnology, Inc. Phosphin-enthaltende formulierung für chemilumineszente luciferase nachweisverfahren
US7030403B2 (en) * 2001-12-06 2006-04-18 Biocontrol Systems, Inc. Sample collection and bioluminescent sample testing system
US20030209653A1 (en) * 2002-04-24 2003-11-13 Biocontrol Systems, Inc. Sample collection and testing system
EP1660508A2 (de) * 2003-08-15 2006-05-31 Celsis Inc. Verwendung von neuen verbindungen zur freisetzung von nukleotiden aus lebenden zellen
US20050070701A1 (en) * 2003-09-29 2005-03-31 Hochstetler Spencer Erich Detection of living cells in polymers or pigments
JP5255273B2 (ja) * 2004-07-02 2013-08-07 プロメガ コーポレイション 微生物ａｔｐの抽出及び検出システム
US7556933B2 (en) * 2004-10-01 2009-07-07 Luminultra Technologies Ltd. Reagent system and process for adenosine triphosphate monitoring
US8304206B2 (en) 2005-12-02 2012-11-06 Sirtris Pharmaceuticals, Inc. Mass spectrometry assays for identifying compounds that activate deacetylases
WO2008045121A1 (en) * 2006-10-13 2008-04-17 Charm Sciences, Inc. Method and apparatus for reducing luminescent test result interferences
US7829280B2 (en) * 2007-01-25 2010-11-09 Luminultra Technologies Ltd. Process for reducing interferences in luciferase enzyme assays
US20100116735A1 (en) * 2008-11-13 2010-05-13 Yu F Philip Method of monitoring and controlling biological activity in boiler condensate and feedwater systems
JP2012511317A (ja) * 2008-12-12 2012-05-24 ウロジャンテック、ソシエテ、アノニム 核酸増幅反応の特異性、感度および収量を改善するためのシクロデキストリンの使用
US9446406B2 (en) 2012-06-29 2016-09-20 Biocontrol Systems, Inc. Sample collection and bioluminescent analysis system
BR112015021442A2 (pt) * 2013-03-06 2017-07-18 Ruhof Corp dispositivos e métodos para testar a limpeza de instrumentos médicos
US20240192100A1 (en) * 2021-04-01 2024-06-13 Fujirebio Inc. Method for treating biological sample

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1604249A (en) * 1977-05-31 1981-12-02 Kolehmainen S Selective measurement of somatic and microbial cells
US4956274A (en) * 1987-04-06 1990-09-11 Microgenics Corporation Reagent stabilization in enzyme-donor and acceptor assay
EP0301847A1 (de) * 1987-07-29 1989-02-01 Microgenics Corporation Reinigung unter Verwendung von Cyclodextrin
GB8722252D0 (en) * 1987-09-22 1987-10-28 Brewing Res Found Atp extraction
US5032503A (en) * 1988-06-22 1991-07-16 Microgenics Corporation Liquid single reagent for air enzyme complementation assay
US5231015A (en) * 1989-10-18 1993-07-27 Eastman Kodak Company Methods of extracting nucleic acids and pcr amplification without using a proteolytic enzyme

Also Published As

Publication number Publication date
JPH06504200A (ja) 1994-05-19
ES2106170T3 (es) 1997-11-01
US5558986A (en) 1996-09-24
WO1992012253A1 (en) 1992-07-23
JP3140057B2 (ja) 2001-03-05
DE69221604D1 (de) 1997-09-18
DE69221604T2 (de) 1998-03-12
EP0566625B1 (de) 1997-08-13
DK0566625T3 (da) 1998-03-09
GB9100551D0 (en) 1991-02-20
EP0566625A1 (de) 1993-10-27

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