ES2106170T3 - Metodo para la extraccion de componentes intracelulares. - Google Patents
Metodo para la extraccion de componentes intracelulares.Info
- Publication number
- ES2106170T3 ES2106170T3 ES92902694T ES92902694T ES2106170T3 ES 2106170 T3 ES2106170 T3 ES 2106170T3 ES 92902694 T ES92902694 T ES 92902694T ES 92902694 T ES92902694 T ES 92902694T ES 2106170 T3 ES2106170 T3 ES 2106170T3
- Authority
- ES
- Spain
- Prior art keywords
- extractant
- solution
- intracellular components
- extraction
- cyclodextrin
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/66—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving luciferase
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/81—Packaged device or kit
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/10—Composition for standardization, calibration, simulation, stabilization, preparation or preservation; processes of use in preparation for chemical testing
- Y10T436/107497—Preparation composition [e.g., lysing or precipitation, etc.]
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
UN METODO PARA PREPARAR UN EXTRACTO DE UN COMPONENTE INTRACELULAR DONDE LAS CELULAS SON CONTACTADAS CON UN EXTACTANTE PARA GENERAR UNA SOLUCION DE EXTRACTO, SE CARACTERIZA POR CONTACTAR LA SOLUCION CON UNA CICLODEXTRINA PARA NEUTRALIZAR EL EXTRACTANTR.LOS MEJORES EXTRACTANTES SON LOS CATIONICOS O DE OTRO TIPO.LA CICLODEXTRINA SE UTILIZA PREFERENTEMENTE EN SOLUCION.LOS MEJORES COMPONENTES INTRACELULARES SON LOS ATP, LOS CUALES PUEDEN, TRAS LA NEUTRALIZACION DE LOS EXTACTANTES,PASAR POR UN ENSAYO REGULADOR DE LLAMA DE LUCIFERASA; Y LOS ADN O ARN QUE PUEDEN, TRAS LA NEUTRALIZACION DEL SURFACTANTE,SER AMPLIADOS O POSTERIORMENTE PROCESADOS DE OTRAS FORMAS.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GB919100551A GB9100551D0 (en) | 1991-01-10 | 1991-01-10 | Method and reagent for eliminating analytical interference in enzymatic analysis from substances used for extraction of intracellular metabolites |
Publications (1)
Publication Number | Publication Date |
---|---|
ES2106170T3 true ES2106170T3 (es) | 1997-11-01 |
Family
ID=10688253
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES92902694T Expired - Lifetime ES2106170T3 (es) | 1991-01-10 | 1992-01-10 | Metodo para la extraccion de componentes intracelulares. |
Country Status (9)
Country | Link |
---|---|
US (1) | US5558986A (es) |
EP (1) | EP0566625B1 (es) |
JP (1) | JP3140057B2 (es) |
AT (1) | ATE156861T1 (es) |
DE (1) | DE69221604T2 (es) |
DK (1) | DK0566625T3 (es) |
ES (1) | ES2106170T3 (es) |
GB (1) | GB9100551D0 (es) |
WO (1) | WO1992012253A1 (es) |
Families Citing this family (33)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5705345A (en) * | 1991-01-10 | 1998-01-06 | Amersham International Plc | Methods and kits for preparing nucleic acids using cyclodextrin |
US5538870A (en) * | 1994-09-21 | 1996-07-23 | Boehringer Mannheim Corporation | Method for preparing nucleic acids for analysis and kits useful therefor |
JP3118528B2 (ja) | 1996-01-26 | 2000-12-18 | 東邦レーヨン株式会社 | ステアリングホイールおよびその製造方法 |
US5905029A (en) * | 1997-02-19 | 1999-05-18 | Fritz Berthold | Method for rapid hygiene testing |
US5916802A (en) * | 1997-02-19 | 1999-06-29 | Fritz Berthold | Device for measuring ATP |
EP0863402B1 (en) * | 1997-03-03 | 2005-09-14 | Amersham Biosciences UK Limited | In-situ cell extraction and assay method |
JP3773151B2 (ja) | 1997-12-03 | 2006-05-10 | キッコーマン株式会社 | 細胞内成分の分析法 |
JP3749628B2 (ja) * | 1997-12-26 | 2006-03-01 | キッコーマン株式会社 | ルシフェラーゼおよびそれを用いる細胞内atpの測定法 |
WO1999039009A1 (en) * | 1998-02-02 | 1999-08-05 | Gentra Systems, Inc. | Processes for isolating, amplifying and characterizing dna |
US6692921B1 (en) * | 1998-02-16 | 2004-02-17 | Amersham Biosciences Uk Limited | Method for measurement of total analyte |
US20050089918A1 (en) * | 1998-02-23 | 2005-04-28 | Amersham Biosciences Uk Limited | In-situ cell extraction and assay method |
DE19838429C2 (de) * | 1998-08-24 | 2001-07-26 | Fraunhofer Ges Forschung | Anordnung zum schnellen Nachweis von mikrobiologischen oder biochemischen Stoffen |
NL1010224C2 (nl) * | 1998-09-30 | 2000-03-31 | Packard Biosciene B V | Werkwijze voor het detecteren van ATP. |
US6653147B2 (en) | 2000-03-31 | 2003-11-25 | Neogen Corporation | Apparatus and method for chemiluminescent assays |
US6927851B2 (en) | 2000-03-31 | 2005-08-09 | Neogen Corporation | Methods and apparatus to improve the sensitivity and reproducibility of bioluminescent analytical methods |
US6660489B2 (en) * | 2000-12-01 | 2003-12-09 | Becton, Dickinson And Company | ATP extraction method |
US7083911B2 (en) * | 2001-02-16 | 2006-08-01 | Promega Corporation | Method for detection of ATP |
JP2005502363A (ja) * | 2001-09-11 | 2005-01-27 | メルク パテント ゲゼルシャフト ミット ベシュレンクテル ハフトング | 酵素アッセイのためのラテラルフローテストフォーマット |
AU2002348287A1 (en) * | 2001-11-16 | 2003-06-10 | Pierce Biotechnology, Inc. | Phosphine-containing formulations for chemiluminescent luciferase assays |
BRPI0214680B1 (pt) * | 2001-12-06 | 2018-09-18 | Biocontrol Systems Inc | instrumento para uso em monitorar um produto, um ingrediente, um ambiente ou processo, instrumento para detectar emissão de luz de uma amostra e método de monitorar uma amostra de um produto, um ingrediente, um processo ou ambiente |
US20030209653A1 (en) * | 2002-04-24 | 2003-11-13 | Biocontrol Systems, Inc. | Sample collection and testing system |
EP1660508A2 (en) * | 2003-08-15 | 2006-05-31 | Celsis Inc. | Use of novel compounds to release nucleotides from living cells |
US20050070701A1 (en) * | 2003-09-29 | 2005-03-31 | Hochstetler Spencer Erich | Detection of living cells in polymers or pigments |
ES2324952T3 (es) * | 2004-07-02 | 2009-08-20 | Promega Corporation | Composiciones y procesos para la extraccion y deteccion de atp microbiano. |
US7556933B2 (en) * | 2004-10-01 | 2009-07-07 | Luminultra Technologies Ltd. | Reagent system and process for adenosine triphosphate monitoring |
ES2389111T3 (es) | 2005-12-02 | 2012-10-23 | Sirtris Pharmaceuticals, Inc. | Ensayos de espectrometría de masas para la actividad acetiltransferasa/desacetilasa |
WO2008045121A1 (en) * | 2006-10-13 | 2008-04-17 | Charm Sciences, Inc. | Method and apparatus for reducing luminescent test result interferences |
US7829280B2 (en) * | 2007-01-25 | 2010-11-09 | Luminultra Technologies Ltd. | Process for reducing interferences in luciferase enzyme assays |
US20100116735A1 (en) * | 2008-11-13 | 2010-05-13 | Yu F Philip | Method of monitoring and controlling biological activity in boiler condensate and feedwater systems |
CN102317472A (zh) * | 2008-12-12 | 2012-01-11 | 欧洲基因技术股份有限公司 | 使用环糊精改进核酸扩增反应的特异性、灵敏度和产率 |
US9446406B2 (en) | 2012-06-29 | 2016-09-20 | Biocontrol Systems, Inc. | Sample collection and bioluminescent analysis system |
JP6382855B2 (ja) * | 2013-03-06 | 2018-08-29 | ルーホフ コーポレイション | 医療機器の清浄度試験装置と方法 |
CN117136304A (zh) * | 2021-04-01 | 2023-11-28 | 富士瑞必欧株式会社 | 生物学样品的处理方法 |
Family Cites Families (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB1604249A (en) * | 1977-05-31 | 1981-12-02 | Kolehmainen S | Selective measurement of somatic and microbial cells |
US4956274A (en) * | 1987-04-06 | 1990-09-11 | Microgenics Corporation | Reagent stabilization in enzyme-donor and acceptor assay |
JPH01126400A (ja) * | 1987-07-29 | 1989-05-18 | Microgenics Corp | シクロデキストリン洗浄組成物 |
GB8722252D0 (en) * | 1987-09-22 | 1987-10-28 | Brewing Res Found | Atp extraction |
US5032503A (en) * | 1988-06-22 | 1991-07-16 | Microgenics Corporation | Liquid single reagent for air enzyme complementation assay |
US5231015A (en) * | 1989-10-18 | 1993-07-27 | Eastman Kodak Company | Methods of extracting nucleic acids and pcr amplification without using a proteolytic enzyme |
-
1991
- 1991-01-10 GB GB919100551A patent/GB9100551D0/en active Pending
-
1992
- 1992-01-10 EP EP92902694A patent/EP0566625B1/en not_active Expired - Lifetime
- 1992-01-10 DE DE69221604T patent/DE69221604T2/de not_active Expired - Lifetime
- 1992-01-10 AT AT92902694T patent/ATE156861T1/de not_active IP Right Cessation
- 1992-01-10 DK DK92902694.6T patent/DK0566625T3/da active
- 1992-01-10 WO PCT/GB1992/000056 patent/WO1992012253A1/en active IP Right Grant
- 1992-01-10 ES ES92902694T patent/ES2106170T3/es not_active Expired - Lifetime
- 1992-01-10 JP JP04502774A patent/JP3140057B2/ja not_active Expired - Lifetime
-
1994
- 1994-11-23 US US08/347,228 patent/US5558986A/en not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
---|---|
GB9100551D0 (en) | 1991-02-20 |
DE69221604D1 (de) | 1997-09-18 |
JPH06504200A (ja) | 1994-05-19 |
DK0566625T3 (da) | 1998-03-09 |
EP0566625B1 (en) | 1997-08-13 |
DE69221604T2 (de) | 1998-03-12 |
ATE156861T1 (de) | 1997-08-15 |
JP3140057B2 (ja) | 2001-03-05 |
WO1992012253A1 (en) | 1992-07-23 |
EP0566625A1 (en) | 1993-10-27 |
US5558986A (en) | 1996-09-24 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
FG2A | Definitive protection |
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