ATE364613T1 - Verfahren zur abtrennung von extrachromosomaler dns aus einer zellsuspension - Google Patents

Verfahren zur abtrennung von extrachromosomaler dns aus einer zellsuspension

Info

Publication number
ATE364613T1
ATE364613T1 AT01997500T AT01997500T ATE364613T1 AT E364613 T1 ATE364613 T1 AT E364613T1 AT 01997500 T AT01997500 T AT 01997500T AT 01997500 T AT01997500 T AT 01997500T AT E364613 T1 ATE364613 T1 AT E364613T1
Authority
AT
Austria
Prior art keywords
separating
cell suspension
extrachromosomal dna
extrachromosomal
dna
Prior art date
Application number
AT01997500T
Other languages
English (en)
Inventor
Henry Charlton
Alex Eon-Duval
Robert Macduff
Original Assignee
Glaxo Group Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Glaxo Group Ltd filed Critical Glaxo Group Ltd
Application granted granted Critical
Publication of ATE364613T1 publication Critical patent/ATE364613T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1017—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by filtration, e.g. using filters, frits, membranes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Biochemistry (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Separation Using Semi-Permeable Membranes (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Saccharide Compounds (AREA)
AT01997500T 2000-11-21 2001-11-20 Verfahren zur abtrennung von extrachromosomaler dns aus einer zellsuspension ATE364613T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GBGB0028361.4A GB0028361D0 (en) 2000-11-21 2000-11-21 Method of separating extra chromosomal dna from other cellular components

Publications (1)

Publication Number Publication Date
ATE364613T1 true ATE364613T1 (de) 2007-07-15

Family

ID=9903579

Family Applications (1)

Application Number Title Priority Date Filing Date
AT01997500T ATE364613T1 (de) 2000-11-21 2001-11-20 Verfahren zur abtrennung von extrachromosomaler dns aus einer zellsuspension

Country Status (13)

Country Link
US (1) US20040076980A1 (de)
EP (1) EP1335926B1 (de)
JP (1) JP4422406B2 (de)
AR (1) AR031482A1 (de)
AT (1) ATE364613T1 (de)
AU (1) AU2002223841A1 (de)
CA (1) CA2428885A1 (de)
DE (1) DE60128945T2 (de)
ES (1) ES2287189T3 (de)
GB (1) GB0028361D0 (de)
MY (1) MY129812A (de)
TW (1) TWI289142B (de)
WO (1) WO2002042317A2 (de)

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WO2006083721A2 (en) * 2005-01-31 2006-08-10 Merck & Co., Inc. Purification process for piasmid dna
US8546127B2 (en) * 2008-06-30 2013-10-01 General Electric Company Bacteria/RNA extraction device
SI2506857T1 (en) 2009-12-01 2018-08-31 Translate Bio, Inc. Delivery of mRNA for the enrichment of proteins and enzymes in human genetic diseases
AT509622A1 (de) * 2010-04-08 2011-10-15 Greiner Bio One Gmbh Verfahren und kit zur trennung viraler und prokaryontischer von eukaryontischen nukleinsäuren
US10597652B2 (en) * 2011-03-29 2020-03-24 Phynexus, Inc. Methods and devices for nucleic acid purification
US11274292B2 (en) 2011-03-29 2022-03-15 Phynexus, Inc. Devices and methods for plasmid purification
US10883100B2 (en) 2011-03-29 2021-01-05 Phynexus, Inc Devices and methods for plasmid purification
KR20220025112A (ko) 2011-06-08 2022-03-03 샤이어 휴먼 지네틱 테라피즈 인크. Mrna 전달을 위한 지질 나노입자 조성물 및 방법
EP3536787A1 (de) 2012-06-08 2019-09-11 Translate Bio, Inc. Nukleaseresistente polynukleotide und verwendungen davon
CN107753954A (zh) 2012-12-13 2018-03-06 免疫医疗公司 功效改进且毒性降低的抗体与sn‑38的免疫缀合物的剂量
WO2015012904A2 (en) 2012-12-13 2015-01-29 Immunomedics, Inc. Antibody-sn-38 immunoconjugates with a cl2a linker
US9931417B2 (en) 2012-12-13 2018-04-03 Immunomedics, Inc. Antibody-SN-38 immunoconjugates with a CL2A linker
EA201591229A1 (ru) * 2013-03-14 2016-01-29 Шир Хьюман Дженетик Терапис, Инк. Способы очистки матричной рнк
KR102311614B1 (ko) 2013-03-14 2021-10-08 샤이어 휴먼 지네틱 테라피즈 인크. Cftr mrna 조성물 및 관련 방법 및 사용
JP6427789B2 (ja) * 2013-07-23 2018-11-28 イミューノメディクス、インコーポレイテッドＩｍｍｕｎｏｍｅｄｉｃｓ， Ｉｎｃ． Ｃｌ２ａリンカーを有する抗体−ｓｎ−３８免疫複合体
EP3574923A1 (de) 2013-10-22 2019-12-04 Translate Bio, Inc. Mrna-therapie für phenylketonurie
ES2707966T3 (es) 2013-10-22 2019-04-08 Translate Bio Inc Terapia de ARNm para la deficiencia en síntesis de argininosuccinato
EP4729528A3 (de) 2014-04-25 2026-06-24 Translate Bio, Inc. Verfahren zur reinigung von messenger-rna
DK3303583T3 (da) 2015-05-29 2020-07-13 Curevac Real Estate Gmbh Fremgangsmåde til fremstilling og oprensning af rna, omfattende mindst et trin til tangential-flow-filtrering
RU2725292C2 (ru) 2016-04-27 2020-06-30 Иммьюномедикс, Инк. Эффективность конъюгатов антитела против trop-2 с лекарственным средством sn-38 для терапии рецидивирующих/рефрактерных к ингибиторам контрольной точки опухолей
MX2019010155A (es) 2017-02-27 2020-12-10 Translate Bio Inc Arnm de cftr optimizado por codón novedoso.
RU2758234C2 (ru) 2017-03-27 2021-10-26 Иммьюномедикс, Инк. ЛЕЧЕНИЕ ТРИЖДЫ НЕГАТИВНОГО РАКА МОЛОЧНОЙ ЖЕЛЕЗЫ, ХАРАКТЕРИЗУЮЩЕГОСЯ ЭКСПРЕССИЕЙ Trop-2, С ПОМОЩЬЮ САЦИТУЗУМАБА ГОВИТЕКАНА И ИНГИБИТОРА Rad51
CA3044082A1 (en) 2017-04-03 2018-10-11 Immunomedics, Inc. Subcutaneous administration of antibody-drug conjugates for cancer therapy
IL270631B2 (en) 2017-05-16 2024-03-01 Translate Bio Inc Treatment of cystic fibrosis by delivery of codon-optimized mrna encoding cftr
US11174500B2 (en) 2018-08-24 2021-11-16 Translate Bio, Inc. Methods for purification of messenger RNA
AU2019384557B2 (en) 2018-11-21 2025-07-17 Translate Bio, Inc. Treatment of cystic fibrosis by delivery of nebulized mRNA encoding CFTR
EP3918065A4 (de) * 2019-01-29 2022-10-26 Flagship Pioneering Innovations V, Inc. Verfahren zur trennung von langen polynukleotiden aus einer zusammensetzung
CA3155003A1 (en) 2019-09-20 2021-03-25 Translate Bio, Inc. Mrna encoding engineered cftr
CN116790578B (zh) * 2023-08-22 2023-12-12 赛奥斯博生物科技(北京)有限公司 一种基于碱裂解法快速纯化质粒的生产工艺
EP4732926A1 (de) * 2024-10-25 2026-04-29 Sartorius BIA Separations d.o.o. Multivalente halogenidsalze zur verwendung in der hydrophoben interaktionschromatographie

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US5561064A (en) * 1994-02-01 1996-10-01 Vical Incorporated Production of pharmaceutical-grade plasmid DNA
DE59709915D1 (de) * 1996-02-06 2003-05-28 Roche Diagnostics Gmbh Verfahren zur herstellung von gereinigter nukleinsäure und deren verwendung
US7026468B2 (en) * 1996-07-19 2006-04-11 Valentis, Inc. Process and equipment for plasmid purification
US7807822B2 (en) * 1996-08-01 2010-10-05 Robert Bridenbaugh Methods for purifying nucleic acids
US6011148A (en) * 1996-08-01 2000-01-04 Megabios Corporation Methods for purifying nucleic acids
EP0853123A1 (de) * 1997-01-10 1998-07-15 Roche Diagnostics GmbH Reinigung von DNA durch Cross-Flow-Filtration
US6214586B1 (en) * 1997-12-08 2001-04-10 Genzyme Corporation Method for purifying plasmid DNA and plasmid DNA substantially free of genomic DNA
US6268492B1 (en) * 1998-08-10 2001-07-31 Chiron Corporation Methods for purifying non-chromosomal nucleic acid molecules from cells
JP2003505077A (ja) * 1999-07-23 2003-02-12 ジェネンテック・インコーポレーテッド 平行流ろ過法を用いるｒｎａｓｅ−及び有機溶媒−非含有プラスミドｄｎａの精製方法
US6747132B2 (en) * 2000-11-29 2004-06-08 Apex Biosciences, Inc. Methods for the synthesis of a modified hemoglobin solution

Also Published As

Publication number Publication date
MY129812A (en) 2007-05-31
US20040076980A1 (en) 2004-04-22
ES2287189T3 (es) 2007-12-16
JP4422406B2 (ja) 2010-02-24
EP1335926A2 (de) 2003-08-20
GB0028361D0 (en) 2001-01-03
JP2004514428A (ja) 2004-05-20
AR031482A1 (es) 2003-09-24
TWI289142B (en) 2007-11-01
WO2002042317A3 (en) 2003-02-06
EP1335926B1 (de) 2007-06-13
DE60128945T2 (de) 2008-02-28
WO2002042317A2 (en) 2002-05-30
AU2002223841A1 (en) 2002-06-03
CA2428885A1 (en) 2002-05-30
DE60128945D1 (de) 2007-07-26

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