DE60128945D1 - Verfahren zur abtrennung von extrachromosomaler dns aus einer zellsuspension - Google Patents
Verfahren zur abtrennung von extrachromosomaler dns aus einer zellsuspensionInfo
- Publication number
- DE60128945D1 DE60128945D1 DE60128945T DE60128945T DE60128945D1 DE 60128945 D1 DE60128945 D1 DE 60128945D1 DE 60128945 T DE60128945 T DE 60128945T DE 60128945 T DE60128945 T DE 60128945T DE 60128945 D1 DE60128945 D1 DE 60128945D1
- Authority
- DE
- Germany
- Prior art keywords
- extracromosomal
- cellususpension
- dna
- separating
- separating extracromosomal
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1017—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by filtration, e.g. using filters, frits, membranes
Landscapes
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biomedical Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Zoology (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Crystallography & Structural Chemistry (AREA)
- Biochemistry (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Separation Using Semi-Permeable Membranes (AREA)
- Peptides Or Proteins (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Saccharide Compounds (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GBGB0028361.4A GB0028361D0 (en) | 2000-11-21 | 2000-11-21 | Method of separating extra chromosomal dna from other cellular components |
| GB0028361 | 2000-11-21 | ||
| PCT/GB2001/005114 WO2002042317A2 (en) | 2000-11-21 | 2001-11-20 | Method of separating extra-chromosomal dna from other cellular components |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| DE60128945D1 true DE60128945D1 (de) | 2007-07-26 |
| DE60128945T2 DE60128945T2 (de) | 2008-02-28 |
Family
ID=9903579
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE60128945T Expired - Lifetime DE60128945T2 (de) | 2000-11-21 | 2001-11-20 | Verfahren zur abtrennung von extrachromosomaler dns aus einer zellsuspension |
Country Status (13)
| Country | Link |
|---|---|
| US (1) | US20040076980A1 (de) |
| EP (1) | EP1335926B1 (de) |
| JP (1) | JP4422406B2 (de) |
| AR (1) | AR031482A1 (de) |
| AT (1) | ATE364613T1 (de) |
| AU (1) | AU2002223841A1 (de) |
| CA (1) | CA2428885A1 (de) |
| DE (1) | DE60128945T2 (de) |
| ES (1) | ES2287189T3 (de) |
| GB (1) | GB0028361D0 (de) |
| MY (1) | MY129812A (de) |
| TW (1) | TWI289142B (de) |
| WO (1) | WO2002042317A2 (de) |
Families Citing this family (31)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB0107634D0 (en) * | 2001-03-27 | 2001-05-16 | Fermentas Ab | Nucleic acid purification |
| WO2006083721A2 (en) * | 2005-01-31 | 2006-08-10 | Merck & Co., Inc. | Purification process for piasmid dna |
| US8546127B2 (en) * | 2008-06-30 | 2013-10-01 | General Electric Company | Bacteria/RNA extraction device |
| SI2506857T1 (en) | 2009-12-01 | 2018-08-31 | Translate Bio, Inc. | Delivery of mRNA for the enrichment of proteins and enzymes in human genetic diseases |
| AT509622A1 (de) * | 2010-04-08 | 2011-10-15 | Greiner Bio One Gmbh | Verfahren und kit zur trennung viraler und prokaryontischer von eukaryontischen nukleinsäuren |
| US10597652B2 (en) * | 2011-03-29 | 2020-03-24 | Phynexus, Inc. | Methods and devices for nucleic acid purification |
| US11274292B2 (en) | 2011-03-29 | 2022-03-15 | Phynexus, Inc. | Devices and methods for plasmid purification |
| US10883100B2 (en) | 2011-03-29 | 2021-01-05 | Phynexus, Inc | Devices and methods for plasmid purification |
| KR20220025112A (ko) | 2011-06-08 | 2022-03-03 | 샤이어 휴먼 지네틱 테라피즈 인크. | Mrna 전달을 위한 지질 나노입자 조성물 및 방법 |
| EP3536787A1 (de) | 2012-06-08 | 2019-09-11 | Translate Bio, Inc. | Nukleaseresistente polynukleotide und verwendungen davon |
| CN107753954A (zh) | 2012-12-13 | 2018-03-06 | 免疫医疗公司 | 功效改进且毒性降低的抗体与sn‑38的免疫缀合物的剂量 |
| WO2015012904A2 (en) | 2012-12-13 | 2015-01-29 | Immunomedics, Inc. | Antibody-sn-38 immunoconjugates with a cl2a linker |
| US9931417B2 (en) | 2012-12-13 | 2018-04-03 | Immunomedics, Inc. | Antibody-SN-38 immunoconjugates with a CL2A linker |
| EA201591229A1 (ru) * | 2013-03-14 | 2016-01-29 | Шир Хьюман Дженетик Терапис, Инк. | Способы очистки матричной рнк |
| KR102311614B1 (ko) | 2013-03-14 | 2021-10-08 | 샤이어 휴먼 지네틱 테라피즈 인크. | Cftr mrna 조성물 및 관련 방법 및 사용 |
| JP6427789B2 (ja) * | 2013-07-23 | 2018-11-28 | イミューノメディクス、インコーポレイテッドＩｍｍｕｎｏｍｅｄｉｃｓ， Ｉｎｃ． | Ｃｌ２ａリンカーを有する抗体−ｓｎ−３８免疫複合体 |
| EP3574923A1 (de) | 2013-10-22 | 2019-12-04 | Translate Bio, Inc. | Mrna-therapie für phenylketonurie |
| ES2707966T3 (es) | 2013-10-22 | 2019-04-08 | Translate Bio Inc | Terapia de ARNm para la deficiencia en síntesis de argininosuccinato |
| EP4729528A3 (de) | 2014-04-25 | 2026-06-24 | Translate Bio, Inc. | Verfahren zur reinigung von messenger-rna |
| DK3303583T3 (da) | 2015-05-29 | 2020-07-13 | Curevac Real Estate Gmbh | Fremgangsmåde til fremstilling og oprensning af rna, omfattende mindst et trin til tangential-flow-filtrering |
| RU2725292C2 (ru) | 2016-04-27 | 2020-06-30 | Иммьюномедикс, Инк. | Эффективность конъюгатов антитела против trop-2 с лекарственным средством sn-38 для терапии рецидивирующих/рефрактерных к ингибиторам контрольной точки опухолей |
| MX2019010155A (es) | 2017-02-27 | 2020-12-10 | Translate Bio Inc | Arnm de cftr optimizado por codón novedoso. |
| RU2758234C2 (ru) | 2017-03-27 | 2021-10-26 | Иммьюномедикс, Инк. | ЛЕЧЕНИЕ ТРИЖДЫ НЕГАТИВНОГО РАКА МОЛОЧНОЙ ЖЕЛЕЗЫ, ХАРАКТЕРИЗУЮЩЕГОСЯ ЭКСПРЕССИЕЙ Trop-2, С ПОМОЩЬЮ САЦИТУЗУМАБА ГОВИТЕКАНА И ИНГИБИТОРА Rad51 |
| CA3044082A1 (en) | 2017-04-03 | 2018-10-11 | Immunomedics, Inc. | Subcutaneous administration of antibody-drug conjugates for cancer therapy |
| IL270631B2 (en) | 2017-05-16 | 2024-03-01 | Translate Bio Inc | Treatment of cystic fibrosis by delivery of codon-optimized mrna encoding cftr |
| US11174500B2 (en) | 2018-08-24 | 2021-11-16 | Translate Bio, Inc. | Methods for purification of messenger RNA |
| AU2019384557B2 (en) | 2018-11-21 | 2025-07-17 | Translate Bio, Inc. | Treatment of cystic fibrosis by delivery of nebulized mRNA encoding CFTR |
| EP3918065A4 (de) * | 2019-01-29 | 2022-10-26 | Flagship Pioneering Innovations V, Inc. | Verfahren zur trennung von langen polynukleotiden aus einer zusammensetzung |
| CA3155003A1 (en) | 2019-09-20 | 2021-03-25 | Translate Bio, Inc. | Mrna encoding engineered cftr |
| CN116790578B (zh) * | 2023-08-22 | 2023-12-12 | 赛奥斯博生物科技(北京)有限公司 | 一种基于碱裂解法快速纯化质粒的生产工艺 |
| EP4732926A1 (de) * | 2024-10-25 | 2026-04-29 | Sartorius BIA Separations d.o.o. | Multivalente halogenidsalze zur verwendung in der hydrophoben interaktionschromatographie |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5561064A (en) * | 1994-02-01 | 1996-10-01 | Vical Incorporated | Production of pharmaceutical-grade plasmid DNA |
| DE59709915D1 (de) * | 1996-02-06 | 2003-05-28 | Roche Diagnostics Gmbh | Verfahren zur herstellung von gereinigter nukleinsäure und deren verwendung |
| US7026468B2 (en) * | 1996-07-19 | 2006-04-11 | Valentis, Inc. | Process and equipment for plasmid purification |
| US7807822B2 (en) * | 1996-08-01 | 2010-10-05 | Robert Bridenbaugh | Methods for purifying nucleic acids |
| US6011148A (en) * | 1996-08-01 | 2000-01-04 | Megabios Corporation | Methods for purifying nucleic acids |
| EP0853123A1 (de) * | 1997-01-10 | 1998-07-15 | Roche Diagnostics GmbH | Reinigung von DNA durch Cross-Flow-Filtration |
| US6214586B1 (en) * | 1997-12-08 | 2001-04-10 | Genzyme Corporation | Method for purifying plasmid DNA and plasmid DNA substantially free of genomic DNA |
| US6268492B1 (en) * | 1998-08-10 | 2001-07-31 | Chiron Corporation | Methods for purifying non-chromosomal nucleic acid molecules from cells |
| JP2003505077A (ja) * | 1999-07-23 | 2003-02-12 | ジェネンテック・インコーポレーテッド | 平行流ろ過法を用いるｒｎａｓｅ−及び有機溶媒−非含有プラスミドｄｎａの精製方法 |
| US6747132B2 (en) * | 2000-11-29 | 2004-06-08 | Apex Biosciences, Inc. | Methods for the synthesis of a modified hemoglobin solution |
-
2000
- 2000-11-21 GB GBGB0028361.4A patent/GB0028361D0/en not_active Ceased
-
2001
- 2001-11-20 WO PCT/GB2001/005114 patent/WO2002042317A2/en not_active Ceased
- 2001-11-20 AT AT01997500T patent/ATE364613T1/de not_active IP Right Cessation
- 2001-11-20 CA CA002428885A patent/CA2428885A1/en not_active Abandoned
- 2001-11-20 EP EP01997500A patent/EP1335926B1/de not_active Expired - Lifetime
- 2001-11-20 JP JP2002544450A patent/JP4422406B2/ja not_active Expired - Fee Related
- 2001-11-20 DE DE60128945T patent/DE60128945T2/de not_active Expired - Lifetime
- 2001-11-20 MY MYPI20015300A patent/MY129812A/en unknown
- 2001-11-20 TW TW090128723A patent/TWI289142B/zh not_active IP Right Cessation
- 2001-11-20 ES ES01997500T patent/ES2287189T3/es not_active Expired - Lifetime
- 2001-11-20 AR ARP010105408A patent/AR031482A1/es unknown
- 2001-11-20 AU AU2002223841A patent/AU2002223841A1/en not_active Abandoned
- 2001-11-22 US US10/432,315 patent/US20040076980A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| MY129812A (en) | 2007-05-31 |
| US20040076980A1 (en) | 2004-04-22 |
| ES2287189T3 (es) | 2007-12-16 |
| JP4422406B2 (ja) | 2010-02-24 |
| EP1335926A2 (de) | 2003-08-20 |
| GB0028361D0 (en) | 2001-01-03 |
| JP2004514428A (ja) | 2004-05-20 |
| AR031482A1 (es) | 2003-09-24 |
| TWI289142B (en) | 2007-11-01 |
| WO2002042317A3 (en) | 2003-02-06 |
| EP1335926B1 (de) | 2007-06-13 |
| DE60128945T2 (de) | 2008-02-28 |
| ATE364613T1 (de) | 2007-07-15 |
| WO2002042317A2 (en) | 2002-05-30 |
| AU2002223841A1 (en) | 2002-06-03 |
| CA2428885A1 (en) | 2002-05-30 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| 8364 | No opposition during term of opposition |