DE60128945D1 - Verfahren zur abtrennung von extrachromosomaler dns aus einer zellsuspension - Google Patents

Verfahren zur abtrennung von extrachromosomaler dns aus einer zellsuspension

Info

Publication number
DE60128945D1
DE60128945D1 DE60128945T DE60128945T DE60128945D1 DE 60128945 D1 DE60128945 D1 DE 60128945D1 DE 60128945 T DE60128945 T DE 60128945T DE 60128945 T DE60128945 T DE 60128945T DE 60128945 D1 DE60128945 D1 DE 60128945D1
Authority
DE
Germany
Prior art keywords
extracromosomal
cellususpension
dna
separating
separating extracromosomal
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60128945T
Other languages
English (en)
Other versions
DE60128945T2 (de
Inventor
Henry Charlton
Alex Eon-Duval
Robert Macduff
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Glaxo Group Ltd
Original Assignee
Glaxo Group Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Glaxo Group Ltd filed Critical Glaxo Group Ltd
Application granted granted Critical
Publication of DE60128945D1 publication Critical patent/DE60128945D1/de
Publication of DE60128945T2 publication Critical patent/DE60128945T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1017—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by filtration, e.g. using filters, frits, membranes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Biochemistry (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Separation Using Semi-Permeable Membranes (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
DE60128945T 2000-11-21 2001-11-20 Verfahren zur abtrennung von extrachromosomaler dns aus einer zellsuspension Expired - Lifetime DE60128945T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB0028361 2000-11-21
GBGB0028361.4A GB0028361D0 (en) 2000-11-21 2000-11-21 Method of separating extra chromosomal dna from other cellular components
PCT/GB2001/005114 WO2002042317A2 (en) 2000-11-21 2001-11-20 Method of separating extra-chromosomal dna from other cellular components

Publications (2)

Publication Number Publication Date
DE60128945D1 true DE60128945D1 (de) 2007-07-26
DE60128945T2 DE60128945T2 (de) 2008-02-28

Family

ID=9903579

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60128945T Expired - Lifetime DE60128945T2 (de) 2000-11-21 2001-11-20 Verfahren zur abtrennung von extrachromosomaler dns aus einer zellsuspension

Country Status (13)

Country Link
US (1) US20040076980A1 (de)
EP (1) EP1335926B1 (de)
JP (1) JP4422406B2 (de)
AR (1) AR031482A1 (de)
AT (1) ATE364613T1 (de)
AU (1) AU2002223841A1 (de)
CA (1) CA2428885A1 (de)
DE (1) DE60128945T2 (de)
ES (1) ES2287189T3 (de)
GB (1) GB0028361D0 (de)
MY (1) MY129812A (de)
TW (1) TWI289142B (de)
WO (1) WO2002042317A2 (de)

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GB0107634D0 (en) * 2001-03-27 2001-05-16 Fermentas Ab Nucleic acid purification
US7767399B2 (en) 2005-01-31 2010-08-03 Merck & Co., Inc. Purification process for plasmid DNA
US8546127B2 (en) * 2008-06-30 2013-10-01 General Electric Company Bacteria/RNA extraction device
HUE038039T2 (hu) 2009-12-01 2018-09-28 Translate Bio Inc mRNS bejuttatása fehérjék és enzimek kiegészítésére humán genetikai betegségekben
AT509622A1 (de) * 2010-04-08 2011-10-15 Greiner Bio One Gmbh Verfahren und kit zur trennung viraler und prokaryontischer von eukaryontischen nukleinsäuren
US11274292B2 (en) 2011-03-29 2022-03-15 Phynexus, Inc. Devices and methods for plasmid purification
US10597652B2 (en) * 2011-03-29 2020-03-24 Phynexus, Inc. Methods and devices for nucleic acid purification
US10883100B2 (en) 2011-03-29 2021-01-05 Phynexus, Inc Devices and methods for plasmid purification
ME03491B (de) 2011-06-08 2020-01-20 Translate Bio Inc Lipidnanoteilchenzusammensetzungen und verfahren zur mrna-freisetzung
EP2859102A4 (de) 2012-06-08 2016-05-11 Shire Human Genetic Therapies Nukleaseresistente polynukleotide und verwendungen davon
US9931417B2 (en) 2012-12-13 2018-04-03 Immunomedics, Inc. Antibody-SN-38 immunoconjugates with a CL2A linker
DK2900277T3 (da) 2012-12-13 2022-04-04 Immunomedics Inc Doser af immunokonjugater af antistoffer og sn-38 til forbedret effektivitet og reduceret toksicitet
WO2015012904A2 (en) 2012-12-13 2015-01-29 Immunomedics, Inc. Antibody-sn-38 immunoconjugates with a cl2a linker
EA037922B1 (ru) 2013-03-14 2021-06-07 Шир Хьюман Дженетик Терапис, Инк. мРНК CFTR И КОМПОЗИЦИИ ДЛЯ ЛЕЧЕНИЯ МУКОВИСЦИДОЗА У МЛЕКОПИТАЮЩЕГО
EP3467108B1 (de) 2013-03-14 2024-05-22 Translate Bio, Inc. Verfahren zur reinigung von messenger-rna
JP6427789B2 (ja) * 2013-07-23 2018-11-28 イミューノメディクス、インコーポレイテッドＩｍｍｕｎｏｍｅｄｉｃｓ， Ｉｎｃ． Ｃｌ２ａリンカーを有する抗体−ｓｎ−３８免疫複合体
AU2014340092B2 (en) 2013-10-22 2019-09-19 Translate Bio, Inc. mRNA therapy for Argininosuccinate Synthetase Deficiency
EP3060258A1 (de) 2013-10-22 2016-08-31 Shire Human Genetic Therapies, Inc. Mrna-therapie für phenylketonurie
EP3636742B1 (de) 2014-04-25 2025-11-05 Translate Bio, Inc. Verfahren zur reinigung von messenger-rna
ES2798271T3 (es) 2015-05-29 2020-12-10 Curevac Real Estate Gmbh Método para producir y purificar ARN, que comprende al menos una etapa de filtración de flujo tangencial
EP3448260A4 (de) 2016-04-27 2019-10-09 Immunomedics, Inc. Wirksamkeit von anti-trop-2-sn-38-antikörper-wirkstoff-konjugaten zur therapie von rezidivierten/refraktären tumoren gegen checkpoint-inhibitoren
EP3585417B1 (de) 2017-02-27 2023-02-22 Translate Bio, Inc. Methode der herstellung von codon-optimierter cftr-mrna
CN110392570A (zh) 2017-03-27 2019-10-29 免疫医疗公司 用沙妥珠单抗格维替康和rad51抑制剂治疗表达trop-2的三阴性乳腺癌
EP3606964A4 (de) 2017-04-03 2020-12-09 Immunomedics, Inc. Subkutane verabreichung von antikörper-arzneimittelkonjugaten zur krebstherapie
CA3063531A1 (en) 2017-05-16 2018-11-22 Translate Bio, Inc. Treatment of cystic fibrosis by delivery of codon-optimized mrna encoding cftr
CN118421617A (zh) 2018-08-24 2024-08-02 川斯勒佰尔公司 用于纯化信使rna的方法
MX2021005969A (es) 2018-11-21 2021-09-14 Translate Bio Inc Tratamiento de la fibrosis quística mediante el suministro de arnm que codifica cftr nebulizado.
EP3918065A4 (de) * 2019-01-29 2022-10-26 Flagship Pioneering Innovations V, Inc. Verfahren zur trennung von langen polynukleotiden aus einer zusammensetzung
CN116790578B (zh) * 2023-08-22 2023-12-12 赛奥斯博生物科技(北京)有限公司 一种基于碱裂解法快速纯化质粒的生产工艺

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5561064A (en) * 1994-02-01 1996-10-01 Vical Incorporated Production of pharmaceutical-grade plasmid DNA
DE59709915D1 (de) * 1996-02-06 2003-05-28 Roche Diagnostics Gmbh Verfahren zur herstellung von gereinigter nukleinsäure und deren verwendung
US7026468B2 (en) * 1996-07-19 2006-04-11 Valentis, Inc. Process and equipment for plasmid purification
US7807822B2 (en) * 1996-08-01 2010-10-05 Robert Bridenbaugh Methods for purifying nucleic acids
US6011148A (en) * 1996-08-01 2000-01-04 Megabios Corporation Methods for purifying nucleic acids
EP0853123A1 (de) * 1997-01-10 1998-07-15 Roche Diagnostics GmbH Reinigung von DNA durch Cross-Flow-Filtration
US6214586B1 (en) * 1997-12-08 2001-04-10 Genzyme Corporation Method for purifying plasmid DNA and plasmid DNA substantially free of genomic DNA
US6268492B1 (en) * 1998-08-10 2001-07-31 Chiron Corporation Methods for purifying non-chromosomal nucleic acid molecules from cells
PT1200578E (pt) * 1999-07-23 2004-08-31 Genentech Inc Metodo para purificacao de adn de plasmideo sem solventes organicos e sem arnase utilizando filtracao de fluxo tangencial
US6747132B2 (en) * 2000-11-29 2004-06-08 Apex Biosciences, Inc. Methods for the synthesis of a modified hemoglobin solution

Also Published As

Publication number Publication date
JP4422406B2 (ja) 2010-02-24
ATE364613T1 (de) 2007-07-15
JP2004514428A (ja) 2004-05-20
CA2428885A1 (en) 2002-05-30
AU2002223841A1 (en) 2002-06-03
EP1335926A2 (de) 2003-08-20
ES2287189T3 (es) 2007-12-16
WO2002042317A3 (en) 2003-02-06
MY129812A (en) 2007-05-31
EP1335926B1 (de) 2007-06-13
GB0028361D0 (en) 2001-01-03
US20040076980A1 (en) 2004-04-22
DE60128945T2 (de) 2008-02-28
TWI289142B (en) 2007-11-01
WO2002042317A2 (en) 2002-05-30
AR031482A1 (es) 2003-09-24

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