ATE360079T1 - Expression heterologer proteine - Google Patents

Expression heterologer proteine

Info

Publication number
ATE360079T1
ATE360079T1 AT98904318T AT98904318T ATE360079T1 AT E360079 T1 ATE360079 T1 AT E360079T1 AT 98904318 T AT98904318 T AT 98904318T AT 98904318 T AT98904318 T AT 98904318T AT E360079 T1 ATE360079 T1 AT E360079T1
Authority
AT
Austria
Prior art keywords
protein
expression
expression system
heterologous proteins
native
Prior art date
Application number
AT98904318T
Other languages
English (en)
Inventor
Cesira Galeotti
Original Assignee
Novartis Vaccines & Diagnostic
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novartis Vaccines & Diagnostic filed Critical Novartis Vaccines & Diagnostic
Application granted granted Critical
Publication of ATE360079T1 publication Critical patent/ATE360079T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A61P37/02—Immunomodulators
    • A61P37/04—Immunostimulants
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/905—Stable introduction of foreign DNA into chromosome using homologous recombination in yeast
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0036—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on NADH or NADPH (1.6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y108/00—Oxidoreductases acting on sulfur groups as donors (1.8)
    • C12Y108/01—Oxidoreductases acting on sulfur groups as donors (1.8) with NAD+ or NADP+ as acceptor (1.8.1)
    • C12Y108/01008—Protein-disulfide reductase (1.8.1.8), i.e. thioredoxin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y503/00—Intramolecular oxidoreductases (5.3)
    • C12Y503/04—Intramolecular oxidoreductases (5.3) transposing S-S bonds (5.3.4)
    • C12Y503/04001—Protein disulfide-isomerase (5.3.4.1), i.e. disufide bond-forming enzyme
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
    • C12N2770/00011—Details
    • C12N2770/36011—Togaviridae
    • C12N2770/36111—Alphavirus, e.g. Sindbis virus, VEE, EEE, WEE, Semliki
    • C12N2770/36122—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Mycology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Immunology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Toxicology (AREA)
  • Virology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Animal Behavior & Ethology (AREA)
  • Veterinary Medicine (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Public Health (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
AT98904318T 1997-02-19 1998-02-18 Expression heterologer proteine ATE360079T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GBGB9703406.0A GB9703406D0 (en) 1997-02-19 1997-02-19 Expression of heterologous proteins

Publications (1)

Publication Number Publication Date
ATE360079T1 true ATE360079T1 (de) 2007-05-15

Family

ID=10807917

Family Applications (1)

Application Number Title Priority Date Filing Date
AT98904318T ATE360079T1 (de) 1997-02-19 1998-02-18 Expression heterologer proteine

Country Status (10)

Country Link
US (2) US6361969B1 (de)
EP (1) EP1007698B1 (de)
JP (2) JP4308916B2 (de)
AT (1) ATE360079T1 (de)
DE (1) DE69837604T2 (de)
DK (1) DK1007698T3 (de)
ES (1) ES2286844T3 (de)
GB (1) GB9703406D0 (de)
PT (1) PT1007698E (de)
WO (1) WO1998037208A1 (de)

Families Citing this family (49)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7803765B2 (en) * 1999-05-05 2010-09-28 Phylogica Limited Methods of constructing biodiverse gene fragment libraries and biological modulators isolated therefrom
US7270969B2 (en) * 1999-05-05 2007-09-18 Phylogica Limited Methods of constructing and screening diverse expression libraries
DE60044514D1 (de) 1999-05-05 2010-07-15 Phylogica Ltd Isolierung von biologischen Modulatoren aus Bibliotheken mit biologisch vielvältigen Genfragmenten
CZ20021819A3 (cs) * 1999-10-27 2003-06-18 Innogenetics N. V. Oxidačně redukční reverzibilní HCV proteiny s konformací podobnou nativním proteinům
US7015033B1 (en) * 2000-05-25 2006-03-21 Aventis Pasteur Limited Co-expression of recombination proteins
US6673613B2 (en) * 2000-07-26 2004-01-06 Cognis Corporation Use of CYP52A2A promoter to increase gene expression in yeast
NZ530632A (en) * 2001-06-29 2007-04-27 Chiron Corp HCV E1E2 vaccine compositions comprising E1E2 antigens, submicron oil-in-water emulsions and/or CpG oligonucleotides
EP1495055B1 (de) * 2002-04-18 2013-08-14 Genencor International, Inc. Herstellung von funktionellen antikörpern in filamentösen pilzen
US7439042B2 (en) * 2002-12-16 2008-10-21 Globeimmune, Inc. Yeast-based therapeutic for chronic hepatitis C infection
GB0329722D0 (en) * 2003-12-23 2004-01-28 Delta Biotechnology Ltd Modified plasmid and use thereof
US9057061B2 (en) 2003-12-23 2015-06-16 Novozymes Biopharma Dk A/S Gene expression technique
US8906676B2 (en) * 2004-02-02 2014-12-09 Ambrx, Inc. Modified human four helical bundle polypeptides and their uses
WO2005119244A1 (en) * 2004-06-03 2005-12-15 Phylogica Limited Peptide modulators of cellular phenotype and bi-nucleic acid fragment library
KR101699142B1 (ko) * 2004-06-18 2017-01-23 암브룩스, 인코포레이티드 신규 항원-결합 폴리펩티드 및 이의 용도
US8597908B2 (en) 2004-07-06 2013-12-03 Kaneka Corporation Process for producing protein A-like protein with use of Brevibacillus genus bacterium
JP2008507280A (ja) * 2004-07-21 2008-03-13 アンブレツクス・インコーポレイテツド 非天然コードアミノ酸を用いた生合成ポリペプチド
AU2005295317B2 (en) * 2004-10-18 2011-10-13 Globeimmune, Inc. Yeast-based therapeutic for chronic hepatitis C infection
CN103520735B (zh) 2004-12-22 2015-11-25 Ambrx公司 包含非天然编码的氨基酸的人生长激素配方
SG158148A1 (en) 2004-12-22 2010-01-29 Ambrx Inc Compositions containing, methods involving, and uses of non-natural amino acids and polypeptides
MX2007007591A (es) 2004-12-22 2007-07-25 Ambrx Inc Metodos para expresion y purificacion de hormona de crecimiento humano recombinante.
JP2008525473A (ja) 2004-12-22 2008-07-17 アンブレツクス・インコーポレイテツド 修飾されたヒト成長ホルモン
CA2590429C (en) * 2004-12-22 2014-10-07 Ambrx, Inc. Compositions of aminoacyl-trna synthetase and uses thereof
US7456011B2 (en) * 2005-01-12 2008-11-25 Osel, Inc. Modified cyanovirin-n polypeptide
JP2008541769A (ja) 2005-06-03 2008-11-27 アンブレツクス・インコーポレイテツド 改善されたヒトインターフェロン分子及びそれらの使用
PT2339014E (pt) 2005-11-16 2015-10-13 Ambrx Inc Métodos e composições compreendendo aminoácidos não-naturais
JP5576610B2 (ja) * 2006-02-20 2014-08-20 フィロジカ リミテッド ペプチド構造のライブラリーの構築およびスクリーニング方法
KR20090060294A (ko) 2006-09-08 2009-06-11 암브룩스, 인코포레이티드 변형된 인간 혈장 폴리펩티드 또는 Ｆｃ 스캐폴드 및 그의 용도
ES2465473T3 (es) 2006-09-08 2014-06-05 Ambrx, Inc. Transcripción de arnt supresor en células de vertebrado
WO2008030613A2 (en) 2006-09-08 2008-03-13 Ambrx, Inc. Hybrid suppressor trna for vertebrate cells
EP2074138A4 (de) * 2006-09-19 2009-12-30 Phylogica Ltd Neuroprotektive peptidinhibitoren der ap-1-signalvermittlung und anwendungen davon
CN104163864B (zh) 2007-03-30 2017-08-01 Ambrx公司 经修饰fgf‑21多肽和其用途
AU2008247815B2 (en) 2007-05-02 2012-09-06 Ambrx, Inc. Modified interferon beta polypeptides and their uses
EP2170932A4 (de) * 2007-06-20 2012-10-10 Phylogica Ltd Zusammensetzungen und anwendungen davon bei der behandlung von akutem atemnotsyndrom (ards) und damit assoziierten klinischen erkrankungen
JP5547083B2 (ja) 2007-11-20 2014-07-09 アンブルックス，インコーポレイテッド 修飾されたインスリンポリペプチドおよびそれらの使用
CN103694337B (zh) 2008-02-08 2016-03-02 Ambrx公司 经修饰瘦素多肽和其用途
UA118536C2 (uk) 2008-07-23 2019-02-11 Амбркс, Інк. Модифікований поліпептид бичачого гранулоцитарного колонієстимулювального фактора та його застосування
JP2012503011A (ja) 2008-09-19 2012-02-02 グローブイミューン，インコーポレイテッド 慢性ｃ型肝炎ウイルス感染の免疫療法
KR101647164B1 (ko) 2008-09-26 2016-08-09 암브룩스, 인코포레이티드 변형된 동물 에리트로포이에틴 폴리펩티드 및 이의 용도
NZ607477A (en) 2008-09-26 2014-09-26 Ambrx Inc Non-natural amino acid replication-dependent microorganisms and vaccines
CN107674121A (zh) 2009-12-21 2018-02-09 Ambrx 公司 经过修饰的牛促生长素多肽和其用途
CN107056929A (zh) 2009-12-21 2017-08-18 Ambrx 公司 经过修饰的猪促生长素多肽和其用途
US9567386B2 (en) 2010-08-17 2017-02-14 Ambrx, Inc. Therapeutic uses of modified relaxin polypeptides
MX346786B (es) 2010-08-17 2017-03-31 Ambrx Inc Polipeptidos de relaxina modificados y sus usos.
AR083006A1 (es) 2010-09-23 2013-01-23 Lilly Co Eli Formulaciones para el factor estimulante de colonias de granulocitos (g-csf) bovino y variantes de las mismas
GB201018664D0 (en) * 2010-11-05 2010-12-22 Msd Biolog Uk Ltd Expression process
PL3412302T3 (pl) 2014-10-24 2021-11-02 Bristol-Myers Squibb Company Zmodyfikowane polipeptydy fgf-21 i ich zastosowania
CN110637027B (zh) 2017-02-08 2024-08-30 百时美施贵宝公司 包含药代动力学增强子的修饰的松弛素多肽及其用途
CN108690139B (zh) * 2017-07-31 2019-03-15 康码（上海）生物科技有限公司 新型融合蛋白的制备及其在提高蛋白质合成的应用
CN113150103A (zh) * 2021-04-01 2021-07-23 芜湖英特菲尔生物制品产业研究院有限公司 一种酿酒酵母表达重组鱼硫氧还蛋白及其制备方法

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5292646A (en) 1991-02-06 1994-03-08 Genetics Institute, Inc. Peptide and protein fusions to thioredoxin and thioredoxin-like molecules
US5646016A (en) 1991-02-06 1997-07-08 Genetics Institute, Inc. Peptide and protein fusions to thioredoxin, thioredoxin-like molecules, and modified thioredoxin-like molecules
US5270181A (en) * 1991-02-06 1993-12-14 Genetics Institute, Inc. Peptide and protein fusions to thioredoxin and thioredoxin-like molecules
EP0509841A3 (en) 1991-04-18 1993-08-18 Tonen Corporation Co-expression system of protein disulfide isomerase gene and useful polypeptide gene and process for producing the polypeptide using its system
US6291205B1 (en) 1992-06-12 2001-09-18 Merck & Co., Inc. Method of increasing production of disulfide bonded recombinant proteins by saccharomyces cerevisiae
WO1994008012A1 (en) * 1992-10-02 1994-04-14 Research Corporation Technologies, Inc. Methods for increasing secretion of overexpressed proteins
JP3092904B2 (ja) * 1995-10-13 2000-09-25 株式会社エイチ・エス・ピー研究所 細菌により可溶性蛋白質を生産する方法
AU2922397A (en) * 1996-04-30 1997-11-19 Schering Corporation Expression of soluble heterologous proteins in bacteria utilizing a thioredoxin/protein expression vector
US5831049A (en) * 1997-01-03 1998-11-03 Incyte Pharmaceuticals, Inc. Human thioredoxin

Also Published As

Publication number Publication date
PT1007698E (pt) 2007-07-27
EP1007698B1 (de) 2007-04-18
JP2001514490A (ja) 2001-09-11
JP2008173126A (ja) 2008-07-31
JP4308916B2 (ja) 2009-08-05
DE69837604T2 (de) 2008-01-03
ES2286844T3 (es) 2007-12-01
DE69837604D1 (en) 2007-05-31
EP1007698A1 (de) 2000-06-14
WO1998037208A1 (en) 1998-08-27
US6361969B1 (en) 2002-03-26
GB9703406D0 (en) 1997-04-09
US20020146793A1 (en) 2002-10-10
DK1007698T3 (da) 2007-08-13

Similar Documents

Publication Publication Date Title
ATE360079T1 (de) Expression heterologer proteine
Gaertner et al. A cluster-gene: evidence for one gene, one polypeptide, five enzymes
NZ335628A (en) Improved expression vectors comprising a nucleic acid construct with tac and gal bacterial promoters
EP1000160B1 (de) Klonierung der prolyl-dipeptidyl-peptidase von aspergillus oryzae
BR0013549A (pt) Enzimas fitase, ácidos nucléicos codificando enzimas fitase e vetores e células hospedeiras incorporando as mesmas
NO872932L (no) Fremgangsmaate for fremstilling av proteiner med faktorviiiaktivitet ved hjelp av mikrobielle vertsceller, eksprimeringsvektorer, vertsceller, antibiotika.
AU3246300A (en) Polypeptides having galactose oxidase activity and nucleic acids encoding same
AU560340B2 (en) Rennin from recombinant dna
NZ509633A (en) A recombinant chimeric PEG-uricase with a long circulatory life and reduced immunogenicity
ES2255281T3 (es) Sobreexpresion de genes de fitasa en sistmas de levadura.
DE69938129D1 (de) Verbesserte in vivo-produktion von cephalosporinen
AU6801487A (en) Method of producing a recombinant protein complex having human factor VIII:C activity
AU2872089A (en) Receptor protein for human b cell stimulatory factor-2
DK0830376T3 (da) Fremgangsmåde til fremstilling af erythropoietin, som er fri for animalske proteiner
WO2003008437A3 (en) Protein and nucleic acid expression systems
ATE452202T1 (de) Glycoengineering in pilzen
PL312828A1 (en) Nucleic acid sequence for encoding a protein a. thaliana of delta-7 activity of delta-5,7-sterole reductase, method of cloning such nucleic acid, delta-7red protein, method of obtaining such protein, antibody, expression vector, transformed yeast strain, transformed host cells as well as application methods
CA1341437C (en) METHOD OF USING EUKARYOTIC EXPRESSION VECTORS COMPRISING A BK VIRUS AMPLIFIER
HUP0203221A2 (hu) A lignin bioszintézisében résztvevő enzimeket kódoló gének és alkalmazásuk
WO2003008449A8 (en) Ntb-a, a surface molecule involved in natural killer cells activity
EP0363127A3 (de) Expression in Eukaryoten
WO2000006713A8 (en) Methods for recombinant protein production in mammalian cells comprising co-expression with fetuin
EP1576096A4 (de) N-acetylglucosaminyltransferase vb codierende sequenz, rekombinante zellen und verfahren
IE900685L (en) Improved tissue culture method
AU2182801A (en) Method for producing adherent animal cells

Legal Events

Date Code Title Description
UEP Publication of translation of european patent specification

Ref document number: 1007698

Country of ref document: EP