ATE360072T1 - Expression eukarotischer peptide in pflanzenplastiden - Google Patents

Expression eukarotischer peptide in pflanzenplastiden

Info

Publication number
ATE360072T1
ATE360072T1 AT99933798T AT99933798T ATE360072T1 AT E360072 T1 ATE360072 T1 AT E360072T1 AT 99933798 T AT99933798 T AT 99933798T AT 99933798 T AT99933798 T AT 99933798T AT E360072 T1 ATE360072 T1 AT E360072T1
Authority
AT
Austria
Prior art keywords
plastid
plant
eukaryotic
peptides
constructs
Prior art date
Application number
AT99933798T
Other languages
English (en)
Inventor
Kevin E Mcbride
Narender Nehra
Douglas A Russell
David M Stalker
Jeffrey M Staub
Original Assignee
Calgene Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US09/113,244 external-priority patent/US6512162B2/en
Application filed by Calgene Llc filed Critical Calgene Llc
Application granted granted Critical
Publication of ATE360072T1 publication Critical patent/ATE360072T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/61—Growth hormone [GH], i.e. somatotropin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/81—Protease inhibitors
    • C07K14/8107—Endopeptidase (E.C. 3.4.21-99) inhibitors
    • C07K14/811—Serine protease (E.C. 3.4.21) inhibitors
    • C07K14/8114—Kunitz type inhibitors
    • C07K14/8117—Bovine/basic pancreatic trypsin inhibitor (BPTI, aprotinin)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8214—Plastid transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C12N15/8275—Glyphosate
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Endocrinology (AREA)
  • Toxicology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Cultivation Of Plants (AREA)
AT99933798T 1998-07-10 1999-07-10 Expression eukarotischer peptide in pflanzenplastiden ATE360072T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US09/113,244 US6512162B2 (en) 1998-07-10 1998-07-10 Expression of eukaryotic peptides in plant plastids
US31684799A 1999-05-21 1999-05-21

Publications (1)

Publication Number Publication Date
ATE360072T1 true ATE360072T1 (de) 2007-05-15

Family

ID=26810845

Family Applications (1)

Application Number Title Priority Date Filing Date
AT99933798T ATE360072T1 (de) 1998-07-10 1999-07-10 Expression eukarotischer peptide in pflanzenplastiden

Country Status (11)

Country Link
US (2) US6812379B2 (de)
EP (1) EP1097211B1 (de)
JP (1) JP2002520019A (de)
AT (1) ATE360072T1 (de)
BR (1) BR9912019A (de)
CA (1) CA2333148A1 (de)
DE (1) DE69935857T2 (de)
DK (1) DK1097211T3 (de)
ES (1) ES2286886T3 (de)
PT (1) PT1097211E (de)
WO (1) WO2000003012A2 (de)

Families Citing this family (66)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7741536B2 (en) 1997-08-07 2010-06-22 University Of Central Florida Research Foundation, Inc. Expression of human serum albumin in plastids
US20100251425A9 (en) 1998-05-15 2010-09-30 University Of Central Florida Expression of human interferon in transgenic chloroplasts
TR200101032T2 (tr) * 1998-10-07 2001-10-22 Syngenta Participations Ag Bitkilerdeki sağaltıcı yönden aktif proteinler.
BR0011293A (pt) * 1999-05-13 2002-02-26 Monsanto Technology Llc Expressão de sedoheptulose 1,7 bisfosfatase em plantas transgênicas
US6815580B1 (en) 1999-05-13 2004-11-09 Monsanto Technology Llc Expression of the Chlorella sorokiniana sedoheptulose 1,7-bisphosphatase in transgenic plants
US20030041353A1 (en) 2001-04-18 2003-02-27 Henry Daniell Mutiple gene expression for engineering novel pathways and hyperexpression of foreign proteins in plants
AU2001245358A1 (en) * 2000-02-29 2001-09-12 Auburn University Production of antibodies in transgenic plastids
WO2001064024A1 (en) * 2000-02-29 2001-09-07 Auburn University Multiple gene expression for engineering novel pathways and hyperexpression of foreign proteins in plants
ATE510916T1 (de) * 2000-03-01 2011-06-15 Univ Auburn Plastidtransformationsvektoren zur expression von proinsulin, das an die cholera toxin b- untereinheit fusioniert ist, in pflanzen
EP1272649A2 (de) * 2000-04-03 2003-01-08 Monsanto Technology LLC Verfahren zur herstellung authentischer cytokine
JP4552280B2 (ja) 2000-06-14 2010-09-29 ソニー株式会社 テレビ受信システム、選局装置および表示装置
GB2364705A (en) * 2000-07-14 2002-02-06 Icgeb Fusion protein expression in plastids
JP4838434B2 (ja) * 2001-03-22 2011-12-14 財団法人地球環境産業技術研究機構 植物体を用いたタンパク質発現系
WO2003091413A2 (en) * 2002-04-23 2003-11-06 The Scripps Research Institute Expression of polypeptides in chloroplasts, and compositions and methods for expressing same
ATE516295T1 (de) 2004-01-20 2011-07-15 Monsanto Technology Llc Chimäre promotoren zur verwendung in pflanzen
CA2613697A1 (en) * 2005-06-28 2007-01-04 Ventria Bioscience Components of cell culture media produced from plant cells
BRPI0617006A8 (pt) * 2005-09-16 2017-01-24 Bayer Cropscience Sa Gene quimérico, vetor designado para transformação de plastídeos vegetais, célula vegetal transplastômica, planta transplastômica, partes colhíveis de planta, método de produção de proteína de interesse em célula vegetal e métodos de produção de peptídeo
EP2371964A1 (de) 2006-05-16 2011-10-05 Monsanto Technology LLC Verwendung einer nichtagrobakteriellen Spezies zur Pflanzenumwandlung
US7838729B2 (en) 2007-02-26 2010-11-23 Monsanto Technology Llc Chloroplast transit peptides for efficient targeting of DMO and uses thereof
CN105219694A (zh) 2007-03-09 2016-01-06 孟山都技术公司 用于转化的植物胚外植体的制备和用途
US8153863B2 (en) 2007-03-23 2012-04-10 New York University Transgenic plants expressing GLK1 and CCA1 having increased nitrogen assimilation capacity
WO2008150461A2 (en) 2007-06-01 2008-12-11 Sapphire Energy High throughput screening of genetically modified photosynthetic organisms
CA2698289A1 (en) * 2007-09-11 2009-03-19 Sapphire Energy, Inc. Methods of producing organic products with photosynthetic organisms and products and compositions thereof
KR20100082837A (ko) * 2007-09-11 2010-07-20 사파이어 에너지, 인크. 광합성 생물들에 의한 분자 생산
US10689633B2 (en) 2008-02-29 2020-06-23 The Trustees Of The University Of Pennsylvania Expression of β-mannanase in chloroplasts and its utilization in lignocellulosic woody biomass hydrolysis
CN103333894B (zh) 2008-04-07 2016-11-23 孟山都技术公司 植物调控元件及其应用
SG173469A1 (en) 2009-02-20 2011-09-29 Ventria Bioscience Cell culture media containing combinations of proteins
US20120277117A1 (en) 2009-02-27 2012-11-01 Adel Zayed Hydroponic apparatus and methods of use
EP2524045B1 (de) 2010-01-14 2015-12-02 Monsanto Technology LLC Pflanzenregulierungselemente und verwendungen davon
RS57012B1 (sr) 2010-08-30 2018-05-31 Dow Agrosciences Llc Pospešivač bakterijskog virusa šećerne repe (scbv) i njegova upotreba u funkcionalnoj genomici biljke
WO2012083137A1 (en) 2010-12-17 2012-06-21 Monsanto Technology Llc Methods for improving competency of plant cells
MX356792B (es) 2011-03-25 2018-06-12 Monsanto Technology Llc Elementos de regulacion de plantas y sus usos.
EA031636B1 (ru) 2011-05-13 2019-01-31 Монсанто Текнолоджи Ллс Регуляторные элементы растений и их применение
CN104411827B (zh) 2012-02-29 2018-01-02 陶氏益农公司 甘蔗杆状病毒(scbv)增强子及其在植物功能基因组学中的用途
US9663793B2 (en) 2012-04-20 2017-05-30 Monsanto Technology, Llc Plant regulatory elements and uses thereof
KR102273360B1 (ko) 2012-12-19 2021-07-06 몬산토 테크놀로지 엘엘씨 식물 조절 요소 및 그의 용도
BR122020011572B1 (pt) 2013-03-14 2021-10-13 Monsanto Technology Llc Cassete de expressão compreendendo elemento regulador de planta e método para produzir uma planta transgênica
JP2016514964A (ja) 2013-03-14 2016-05-26 モンサント テクノロジー エルエルシー 植物調節エレメントおよびその使用
US11519011B2 (en) 2015-03-26 2022-12-06 The Texas A&M University System Conversion of lignin into bioplastics and lipid fuels
US11732269B2 (en) 2015-10-02 2023-08-22 Monsanto Technology Llc Recombinant maize B chromosome sequence and uses thereof
EP3426788B1 (de) 2016-03-11 2021-09-08 Monsanto Technology LLC Pflanzenregulierungselemente und verwendungen davon
PH12018502447B1 (en) 2016-05-24 2023-08-02 Monsanto Technology Llc Plant regulatory elements and uses thereof
CA3029271A1 (en) 2016-06-28 2018-01-04 Cellectis Altering expression of gene products in plants through targeted insertion of nucleic acid sequences
EP3475427A4 (de) 2016-06-28 2019-11-06 Monsanto Technology LLC Verfahren und zusammensetzungen zur verwendung bei der genommodifizierung in pflanzen
WO2018136594A1 (en) 2017-01-19 2018-07-26 Monsanto Technology Llc Plant regulatory elements and uses thereof
WO2019139616A1 (en) 2018-01-12 2019-07-18 The Texas A&M University System Increasing plant bioproduct yield
BR112021003899A2 (pt) 2019-01-10 2021-08-03 Monsanto Technology Llc elementos reguladores de plantas e seus usos
CN110065267B (zh) 2019-04-26 2021-03-26 京东方科技集团股份有限公司 可形变材料、形变结构、Micro-LED显示装置、应变传感器
CN120718905A (zh) 2019-05-29 2025-09-30 孟山都技术公司 使用基因组编辑产生显性等位基因的方法和组合物
EP3872088A1 (de) * 2020-02-28 2021-09-01 Alganelle Verwendung von aprotinin als träger zum herstellen eines rekombinanten proteins, polypeptides oder peptids in algen
EP4314289A1 (de) 2021-03-26 2024-02-07 Flagship Pioneering Innovations VII, LLC Herstellung von zirkulären polyribonukleotiden in einem prokaryotischen system
EP4314277A1 (de) 2021-03-26 2024-02-07 Flagship Pioneering Innovations VII, LLC Zusammensetzungen und verfahren zur herstellung von zirkulären polyribonukleotiden
WO2022204464A1 (en) 2021-03-26 2022-09-29 Flagship Pioneering Innovations Vii, Llc Production of circular polyribonucleotides in a eukaryotic system
EP4426842A1 (de) 2021-11-01 2024-09-11 Flagship Pioneering Innovations VII, LLC Polynukleotide zur modifizierung von organismen
MX2024009021A (es) 2022-01-20 2024-08-06 Flagship Pioneering Innovations Vii Llc Polinucleotidos para modificar organismos.
AU2023337091A1 (en) 2022-09-09 2025-03-06 Eidgenössische Technische Hochschule Zürich Plant regulatory elements and uses thereof
EP4705477A1 (de) 2023-05-03 2026-03-11 Flagship Pioneering Innovations VII, LLC Endornavirale satelliten-rna-amplifikationssysteme für pflanzen
CN121666452A (zh) 2023-05-03 2026-03-13 旗舰创业创新第七有限责任公司 人工马特利病毒目卫星rna
WO2024229395A1 (en) 2023-05-03 2024-11-07 Flagship Pioneering Innovations Vii, Llc Partitiviral satellite rna amplification systems for plants
AR132588A1 (es) 2023-05-03 2025-07-16 Flagship Pioneering Innovations Vii Llc Arn satélite de tymovirales artificiales
WO2024229351A1 (en) 2023-05-03 2024-11-07 Flagship Pioneering Innovations Vii, Llc Artificial secoviridae satellite rnas
CN121666453A (zh) 2023-05-03 2026-03-13 旗舰创业创新第七有限责任公司 人工南方菜豆花叶病病毒科卫星rna
AR132586A1 (es) 2023-05-03 2025-07-16 Flagship Pioneering Innovations Vii Llc Arn satélite de amalgavirus artificiales
WO2024229385A1 (en) 2023-05-03 2024-11-07 Flagship Pioneering Innovations Vii, Llc Artificial ghabrivirales satellite rnas
EP4705470A1 (de) 2023-05-03 2026-03-11 Flagship Pioneering Innovations VII, LLC Künstliche tombusviridae-satelliten-rnas
US20250075226A1 (en) 2023-08-29 2025-03-06 University Of Freiburg Proteins for regulation of symbiotic infection and associated regulatory elements

Family Cites Families (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE247708T1 (de) * 1986-10-02 2003-09-15 Massachusetts Inst Technology Verfahren zur regulierung der metabolischen stabilität von proteinen
US5132213A (en) * 1986-10-02 1992-07-21 Massachusetts Institute Of Technology Method for producing proteins and polypeptides using ubiquitin fusions
JP2753274B2 (ja) * 1988-08-24 1998-05-18 株式会社三和化学研究所 モチリン様ポリペプチドの製法並びにそのための組換えｄｎａ及び発現用プラスミド
US5693507A (en) * 1988-09-26 1997-12-02 Auburn University Genetic engineering of plant chloroplasts
GB8918496D0 (en) * 1989-08-14 1989-09-20 Cambridge Advanced Tech Thylakoid targeting sequence
US5959177A (en) * 1989-10-27 1999-09-28 The Scripps Research Institute Transgenic plants expressing assembled secretory antibodies
KR910014790A (ko) * 1990-01-10 1991-08-31 한태희 랩탑 컴퓨터의 기능선택방법 및 인터페이스회로
EP0519011A1 (de) * 1990-03-05 1992-12-23 The Upjohn Company Protein-expression durch verwendung von samenspezifischen regulierungssequenzen
US5877402A (en) * 1990-05-01 1999-03-02 Rutgers, The State University Of New Jersey DNA constructs and methods for stably transforming plastids of multicellular plants and expressing recombinant proteins therein
US5773705A (en) * 1992-12-31 1998-06-30 Wisconsin Alumni Research Foundation Ubiquitin fusion protein system for protein production in plants
US5576198A (en) * 1993-12-14 1996-11-19 Calgene, Inc. Controlled expression of transgenic constructs in plant plastids
US5545818A (en) * 1994-03-11 1996-08-13 Calgene Inc. Expression of Bacillus thuringiensis cry proteins in plant plastids
US5723755A (en) * 1995-05-16 1998-03-03 Francis E. Lefaivre Large scale production of human or animal proteins using plant bioreactors
US5824870A (en) * 1995-11-06 1998-10-20 Baszczynski; Chris Commercial production of aprotinin in plants
CN1228123A (zh) * 1996-08-14 1999-09-08 诺瓦提斯公司 对植物病原真菌有抑制活性的肽
ATE299941T1 (de) * 1996-09-12 2005-08-15 Syngenta Participations Ag Cellulolytische enzyme-exprimierende transgene pflanzen
CA2188220A1 (en) * 1996-10-18 1998-04-18 Kimberley Kenward Non-food crop plant bioreactor
US6121436A (en) * 1996-12-13 2000-09-19 Monsanto Company Antifungal polypeptide and methods for controlling plant pathogenic fungi
JP2002513283A (ja) * 1997-01-17 2002-05-08 ザ スクリップス リサーチ インスティテュート 組換え分子の発現に有用なｒｎａ結合タンパク質及び結合部位
AU735383B2 (en) * 1997-06-17 2001-07-05 Monsanto Technology Llc Expression of fructose 1,6 bisphosphate aldolase in transgenic plants
WO1999010513A1 (en) * 1997-08-07 1999-03-04 Auburn University Universal chloroplast integration and expression vectors, transformed plants and products thereof

Also Published As

Publication number Publication date
US20030033636A1 (en) 2003-02-13
BR9912019A (pt) 2002-02-19
WO2000003012A3 (en) 2000-06-08
DE69935857T2 (de) 2008-01-10
WO2000003012A9 (en) 2000-07-20
DE69935857D1 (de) 2007-05-31
US6812379B2 (en) 2004-11-02
US20050283848A1 (en) 2005-12-22
US7259293B2 (en) 2007-08-21
CA2333148A1 (en) 2000-01-20
JP2002520019A (ja) 2002-07-09
EP1097211B1 (de) 2007-04-18
WO2000003012A2 (en) 2000-01-20
DK1097211T3 (da) 2007-06-04
ES2286886T3 (es) 2007-12-01
PT1097211E (pt) 2007-07-12
EP1097211A2 (de) 2001-05-09

Similar Documents

Publication Publication Date Title
ATE360072T1 (de) Expression eukarotischer peptide in pflanzenplastiden
Yamanaka et al. A possible interaction of thioredoxin with VDUP1 in HeLa cells detected in a yeast two-hybrid system
WO2000075350A3 (en) Nucleic acid sequences encoding proteins involved in fatty acid beta-oxidation and methods of use
Keiper et al. Protein synthesis initiation factor 4G
DE69830312D1 (de) Methode zur spezifischen integration von genen in säugetierzellen durch homologe rekombination, und vektoren zu deren durchführung
AR020331A1 (es) Construccion, y metodo para producir una proteina en una celula de planta
WO1998045456A8 (de) 2-desoxyglucose-6-phosphat (2-dog-6-p) phosphatase dna-sequenzen als selektionsmarker in pflanzen
BR9912498A (pt) Expressão de peptìdios eucarióticos em plastìdio de plantas
ATE271612T1 (de) Expressionskassette zur expression von beliebigen genen in pflanzensamen
ZA200702046B (en) Selection of host cells expressing protein at high levels
EA199900875A1 (ru) Растения с изменённым ростом
CA2672809A1 (en) Vector for expressing recombinant protein and cho cells transfected with same
DK0941318T3 (da) Håndvægtformede konstruerede ekspressionssystemer til genterapi
WO2002006457A3 (en) Novel lipase genes
EA200701023A1 (ru) Отбор клеток-хозяев, экспрессирующих белок на высоких уровнях
ATE220722T1 (de) Kontrollierte expression transgener konstrukte in pflanzenplastiden
DK0898618T3 (da) Fremgangsmåde til tilvejebringelse af hidtil ukendte DNA-sekvenser
ATE526818T1 (de) Gentechnisch hergestellte wasserlinse
DE60043428D1 (de) POLYPEPTIDE MIT VERZWEIGUNGSENZYM-AKTIVITäT UND FüR DIESE KODIERENDE NUKLEINSäUREN
WO2003076561A3 (en) Recombinase fusion protein with enhanced cellular uptake
AU7093601A (en) Nucleic acid molecules encoding plant cell cycle proteins and uses therefor
GB9810999D0 (en) Materials and methods relating to the expression of genes
WO2000028014A3 (en) Plastid transformation of solanaceous plants
RU2481399C2 (ru) Набор последовательностей для таргетинга экспрессии и контроля посттрансляционных модификаций рекомбинантного полипептида
ATE542913T1 (de) Promotorvarianten zur expression von genen in einer pilzzelle

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties