ATE510916T1 - Plastidtransformationsvektoren zur expression von proinsulin, das an die cholera toxin b- untereinheit fusioniert ist, in pflanzen - Google Patents

Plastidtransformationsvektoren zur expression von proinsulin, das an die cholera toxin b- untereinheit fusioniert ist, in pflanzen

Info

Publication number
ATE510916T1
ATE510916T1 AT08020922T AT08020922T ATE510916T1 AT E510916 T1 ATE510916 T1 AT E510916T1 AT 08020922 T AT08020922 T AT 08020922T AT 08020922 T AT08020922 T AT 08020922T AT E510916 T1 ATE510916 T1 AT E510916T1
Authority
AT
Austria
Prior art keywords
plastid
sequence
cholera toxin
flanking
subunit
Prior art date
Application number
AT08020922T
Other languages
English (en)
Inventor
Henry Daniell
Original Assignee
Univ Auburn
Univ Central Florida
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Auburn, Univ Central Florida filed Critical Univ Auburn
Application granted granted Critical
Publication of ATE510916T1 publication Critical patent/ATE510916T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/28—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Vibrionaceae (F)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/62—Insulins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/76—Albumins
    • C07K14/765—Serum albumin, e.g. HSA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8214—Plastid transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C07K2319/21—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a His-tag
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/35—Fusion polypeptide containing a fusion for enhanced stability/folding during expression, e.g. fusions with chaperones or thioredoxin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/50—Fusion polypeptide containing protease site
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/55—Fusion polypeptide containing a fusion with a toxin, e.g. diphteria toxin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
    • C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones
AT08020922T 2000-03-01 2001-02-28 Plastidtransformationsvektoren zur expression von proinsulin, das an die cholera toxin b- untereinheit fusioniert ist, in pflanzen ATE510916T1 (de)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US18598700P 2000-03-01 2000-03-01
US26342401P 2001-01-23 2001-01-23
US26347301P 2001-01-23 2001-01-23
US26366801P 2001-01-23 2001-01-23
US27068101P 2001-02-22 2001-02-22

Publications (1)

Publication Number Publication Date
ATE510916T1 true ATE510916T1 (de) 2011-06-15

Family

ID=27497660

Family Applications (1)

Application Number Title Priority Date Filing Date
AT08020922T ATE510916T1 (de) 2000-03-01 2001-02-28 Plastidtransformationsvektoren zur expression von proinsulin, das an die cholera toxin b- untereinheit fusioniert ist, in pflanzen

Country Status (6)

Country Link
US (1) US20100304476A1 (de)
EP (2) EP2080803B1 (de)
AT (1) ATE510916T1 (de)
AU (1) AU2001276813A1 (de)
CA (1) CA2402066C (de)
WO (1) WO2001072959A2 (de)

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US20100251425A9 (en) 1998-05-15 2010-09-30 University Of Central Florida Expression of human interferon in transgenic chloroplasts
WO2004005521A1 (en) 2002-07-03 2004-01-15 University Of Central Florida Expression of the human igf-1 in transgenic plastids
CA2437868A1 (en) * 2001-02-14 2002-08-22 Ventria Bioscience Expression of human milk proteins in transgenic plants
US7354760B2 (en) 2001-12-26 2008-04-08 University Of Central Florida Research Foundation, Inc. Expression of protective antigens in transgenic chloroplasts
CA2471737C (en) * 2001-12-26 2016-01-26 University Of Central Florida Expression of protective antigens in transgenic chloroplasts and the production of improved vaccines
CA2483337C (en) * 2002-04-23 2015-10-27 The Scripps Research Institute Expression of polypeptides in chloroplasts, and compositions and methods for expressing same
AU2008202053B2 (en) * 2002-04-23 2012-11-01 The Scripps Research Institute Expression of polypeptides in chloroplasts, and compositions and methods for expressing same
EP2308985B1 (de) 2002-07-03 2018-06-06 The Trustees of the University of Pennsylvania Gentechnische Manipulation von Plastiden über somatische Embryogenese
CN1816622B (zh) 2003-03-28 2011-06-22 株式会社三和化学研究所 高产重组蛋白的植物贮藏器官的生产方法以及新型重组蛋白
JP4864701B2 (ja) 2003-06-17 2012-02-01 セムバイオシス ジェネティクス インコーポレイテッド 植物におけるインスリンの製造方法
AU2003253807A1 (en) * 2003-07-03 2005-02-15 University Of Central Florida A chloroplast transgenic approach to express and purify human serum albumin, a protein highly susceptible to proteolytic degradation
EP1885173A4 (de) 2005-05-27 2009-02-18 Univ Central Florida Für die expression pharmazeutischer proteine entwickelte chloroplaste
DK2230311T3 (da) * 2005-06-28 2014-05-19 Ventria Bioscience Bestanddele af celledyrkningsmedier produceret ud fra planteceller
CN1884517B (zh) * 2006-06-08 2010-06-30 武汉禾元生物科技有限公司 利用谷物非储藏蛋白为融合载体在胚乳表达多肽的方法及应用
CN101484582A (zh) * 2006-07-07 2009-07-15 拜耳生物科学股份有限公司 表达靶向内腔蛋白质的转质体植物
AU2008232543B2 (en) * 2007-03-30 2013-01-17 University Of Central Florida Research Foundation, Inc. Chloroplasts engineered to express pharmaceutical proteins in edible plants
NZ581473A (en) 2007-06-01 2012-02-24 Scripps Research Inst Use of genetically modified organisms to generate biomass degrading enzymes
GB2463337B (en) 2007-09-11 2012-11-21 Sapphire Energy Inc Methods of producing organic products with photosynthetic organisms
US8242093B2 (en) 2008-02-07 2012-08-14 Ceregene, Inc. Rescue of photoreceptors by intravitreal administration of an expression vector encoding a therapeutic protein
US10689633B2 (en) 2008-02-29 2020-06-23 The Trustees Of The University Of Pennsylvania Expression of β-mannanase in chloroplasts and its utilization in lignocellulosic woody biomass hydrolysis
EP2303296B1 (de) * 2008-05-27 2019-11-20 The Trustees of the University of Pennsylvania Oral verabreichbarer impfstoff für yersinia pestis
CH699512A2 (de) * 2008-09-05 2010-03-15 Plantcare Ag Verfahren und Vorrichtung zur automatischen Regelung der Bewässerung von Pflanzen.
WO2010096588A2 (en) 2009-02-20 2010-08-26 Ventria Bioscience Cell culture media containing combinations of proteins
US9724400B2 (en) 2009-11-09 2017-08-08 The Trustees Of The University Of Pennsylvania Administration of plant expressed oral tolerance agents
EP2770991B9 (de) 2011-10-24 2017-01-25 The Trustees Of The University Of Pennsylvania Oral verabreichtes plastidexprimiertes choleratoxin-b-untereinheit-exendin 4 zur verwendung in der behandlung von typ-2-diabetes
RU2016100762A (ru) * 2013-06-14 2017-07-20 Джей.Ар. СИМПЛОТ КОМПАНИ Продукция белка в растениях
WO2015058214A1 (en) 2013-10-18 2015-04-23 Trustees Of The University Of Pennsylvania Oral delivery of angiotensin converting enzyme 2 (ace2) or angiotensin-(1-7) bioencapsulated in plant cells
ES2821882T3 (es) 2013-11-15 2021-04-28 Univ Pennsylvania Composiciones para la supresión de la formación de inhibidores contra el factor VIII en pacientes con hemofilia A.
EP3377622A4 (de) 2015-11-16 2019-06-19 The Trustees Of The University Of Pennsylvania Gezielte abgabe von in pflanzenzellen bioverkapselten therapeutischen proteinen an bestimmte zelltypen zur behandlung von krankheiten
WO2023130185A1 (en) * 2022-01-07 2023-07-13 Solar Grants Biotechnology Inc. Method for genetic transformation of plant organelle genome and uses thereof

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US4616078A (en) * 1985-04-08 1986-10-07 Eli Lilly And Company Process for purifying proinsulin-like materials
US4956282A (en) * 1985-07-29 1990-09-11 Calgene, Inc. Mammalian peptide expression in plant cells
US4963665A (en) * 1986-01-07 1990-10-16 Washington University Human preproinsulin-like growth factor I
US5932479A (en) * 1988-09-26 1999-08-03 Auburn University Genetic engineering of plant chloroplasts
US6680426B2 (en) 1991-01-07 2004-01-20 Auburn University Genetic engineering of plant chloroplasts
US5693507A (en) * 1988-09-26 1997-12-02 Auburn University Genetic engineering of plant chloroplasts
US5877402A (en) * 1990-05-01 1999-03-02 Rutgers, The State University Of New Jersey DNA constructs and methods for stably transforming plastids of multicellular plants and expressing recombinant proteins therein
US6004782A (en) * 1995-04-14 1999-12-21 Bioelastics Research Ltd. Hyperexpression of bioelastic polypeptides
ES2401438T3 (es) * 1997-08-07 2013-04-19 Auburn University Vectores universales de integración y de expresión de cloroplastos, plantas transformadas y sus productos
AU1072499A (en) * 1997-10-07 1999-04-27 Loma Linda University Expression of cholera toxin b subunit in transgenic plants and efficacy thereof in oral vaccines
CA2333148A1 (en) * 1998-07-10 2000-01-20 Calgene Llc Expression of eukaryotic peptides in plant plastids
US6541682B1 (en) * 1998-11-12 2003-04-01 Calgene Llc Plastid transformation of solanaceous plants
AU2001245358A1 (en) * 2000-02-29 2001-09-12 Auburn University Production of antibodies in transgenic plastids
CA2401954A1 (en) 2000-03-02 2001-09-07 Auburn University Marker free transgenic plants: engineering the chloroplast genome without the use of antibiotic selection
US7767885B2 (en) 2001-12-26 2010-08-03 University Of Central Florida Research Foundation, Inc. Plastid genetic engineering via somatic embryogenesis
CA2471737C (en) 2001-12-26 2016-01-26 University Of Central Florida Expression of protective antigens in transgenic chloroplasts and the production of improved vaccines
AU2003253781A1 (en) 2002-07-03 2004-01-23 University Of Central Florida Expression of human interferon in transgenic chloroplasts

Also Published As

Publication number Publication date
AU2001276813A1 (en) 2001-10-08
EP1274846A2 (de) 2003-01-15
EP1274846A4 (de) 2004-09-29
WO2001072959A3 (en) 2002-05-10
CA2402066C (en) 2015-09-29
US20100304476A1 (en) 2010-12-02
EP2080803B1 (de) 2011-05-25
EP2080803A1 (de) 2009-07-22
CA2402066A1 (en) 2001-10-04
WO2001072959A2 (en) 2001-10-04

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